• Title/Summary/Keyword: 중국유(中國柚)

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Evaluation of Morphological Traits in Eggplant germplasm (가지 유전자원의 형태적 특성)

  • Seungbum Lee;Kyung Jun Lee;Gyu-Taek Cho;Ik Jang;Eunae Yoo;Sookyeong Lee;SeongHoon Kim;Do Yoon Hyun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.41-41
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    • 2020
  • 가지(Solanum melongena L..)는 가지과 식물로 인도 동부가 원산지로 추정이 되며 우리나라를 포함해서 전세계적으로 널리 이용되고 있다. 가지는 다른 과채에 비해 비타민 함량은 낮으나 무기질과 폴리페놀, 식이섬유가 풍부하고 항균, 항암, 항산화활성을 가지는 것으로 보고되어 있다. 본 연구에서는 국내외에서 수집된 가지 유전자원의 형태적 특성을 조사하여 품종 육성을 위한 기초자료를 제공하고자 하였다. 본 연구에 사용된 가지 자원은 필리핀 78자원, 영국 78자원, 중국 45자원, 한국 12자원 등 16개 국가에서 수집된 459자원을 대상으로 하였다. 가지 유전자원의 생육 특성은 반직립형 437자원으로 가장 많았으며 직립형 15자원, 수평형 7자원으로 조사되었다. 하배축의 안토시아닌은 301자원이 있는 것으로 조사되었고 줄기 안토시아닌 축적은 258자원에서 조사되었다. 가시의 여부는 줄기에 31자원, 잎에 30자원이 조사되었으며 IT189682는 특이적으로 줄기에서만 가시가 조사되었다. 화색의 경우 흰색 31자원, 자주 207자원, 연자주 217자원, 흰자주 4자원으로 조사되었으며 꽃의 크기는 중간이 340자원으로 대부분을 차지했으며 큰 꽃 28자원, 작은 꽃 1자원(IT136568)으로 조사되었다. 과실의 형태는 원형 77자원, 타원형 27자원, 난형 31자원, 서양배형 57자원 곤봉형 88자원, 원통형 84자원, 긴원통형 95자원으로 다양하게 조사되었으며 과실 성숙색은 녹색 94자원, 흰색 16자원, 녹자주 67자원, 백자주 21자원, 자주 240자원, 주황 8자원, 노랑 13자원으로 조사되었다. 과실의 길이는 1.4(IT283605) ~ 40.5cm(K003875)로 평균 17.4cm였으며 두께는 0.7(IT283605) ~ 13.7cm(K162043)으로 평균 5.6cm 조사되었다. 본 실험에서 조사된 가지유전자원의 형태적 특성은 가지 선발에 있어 기초 정보로 활용 가능할 것이며 추가적으로 농업형질, 기능성 물질 분석등의 가지 육종 프로그램을 위한 다양한 평가 및 선발이 필요할 것이다.

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Evaluation of Morphological Traits in Perilla frutescens Germplasm (들깨 유전자원의 형태적 특성)

  • Do Yoon Hyun;Kyung Jun Lee;Gyu-Taek Cho;Seungbum Lee;Ik Jang;Eunae Yoo;Sookyeong Lee;SeongHoon Kim
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.40-40
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    • 2020
  • 들깨(Perilla frutescens L.)는 꿀풀과 1년생 초본으로 한국, 중국 등의 동부 아시아가 원산지로 식용, 약용 및 공업용 등 다양한 용도로 이용되고 있다 들깨는 우리나라에서 오랜시간 재배해 왔으나 체계적인 연구기간이 짧아, 다른 유료작물에 비해 육성품종이 적고 유전자원의 체계적인 수집평가가 이루어지지 않았다. 특히 외국에서는 들깨와 근연종을 이용하여 색소 및 정유성분에 대한 연구를 하는데 비해 우리나라에서는 주로 식용으로만 연구가 진행되고 있다. 들깨 육종에 있어서 중요한 것은 유전자원 확보와 이를 정리 보존하여 우량한 육종재료를 효율적으로 이용하는 것이다. 본 연구에서는 국내에서 수집한 들깨 44자원의 형태적 특성을 조사하여 품종 육성을 위한 기초자료를 제공하고자 하였다. 본 연구에 사용된 들깨 자원은 경북 수집 10자원으로 가장 많았으며 전북 8자원, 경남 7자원, 강원과 경기에서 각각 6자원, 충남 3자원, 전남과 충북에서 각각 2자원이었다. 화색은 들깨 44자원 모두 흰색이었으며 줄기색은 연녹색이 29자원, 녹색 9자원, 자주색 4자원으로 조사되었다. 잎의 앞면은 녹색 27자원, 연녹색 10자원, 진녹색 7자원이었으며 뒷면색은 녹색 26자원, 연녹색 18자원으로 조사되었다. 잎의 모양은 장타원형 21자원, 피침형 15자원, 심장형 7자원이었으며 잎의 모용은 23자원이 적음, 14자원이 보통, 6자원이 많게 조사되었다. 잎의 길이는 35자원이 15.1cm이상으로 조사되었고 잎넓이는 11.1cm이상인 자원이 37자원으로 조사되었다. 종피색은 갈색이 28자원, 회색이 6자원, 암갈색이 3자원 순으로 조사되었다. 본 실험에서 조사된 들깨 유전자원의 형태적 특성은 들깨 선발에 있어 기초 정보로 활용 가능할 것이며 추가적으로 농업형질, 기능성 물질 분석등의 들깨 육종 프로그램을 위한 다양한 평가 및 선발이 필요할 것이다.

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Apoptotic Effects and Cell Cycle Arrest Effects of Extracts from Cnidium monnieri (L.) Cusson through Regulating Akt/mTOR/GSK-3β Signaling Pathways in HCT116 Colon Cancer Cells (HCT116 대장암세포에서 AKT/mTOR/GSK-3β 신호경로 조절을 통한 벌 사상자 추출물(CME)의 apoptosis 및 cell cycle arrest 효과)

  • Lim, Eun Gyeong;Kim, Guen Tae;Kim, Bo Min;Kim, Eun Ji;Ha, Sung Ho;Kim, Sang-Yong;Kim, Young Min
    • Journal of Life Science
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    • v.26 no.6
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    • pp.663-672
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    • 2016
  • The Cnidium monnieri (L.) Cusson is an annual plant distributed in China and Korea. The fruit of C. monnieri is used as a medicinal herb that is effective for the treatment of carbuncle and pain in female genitalia. However, the anti-cancer effects of CME have not yet been reported. In this study, we assessed the apoptotic effects and cell cycle arrest effects of ethanol extracts from C. monnieri on HCT116 colon cancer cells. The results of an MTT assay and LDH assay demonstrated a decrease in cell viability and the cytotoxic effects of CME. In addition, the number of apoptotic body and the apoptotic rate were increased in a dose-dependent manner through Hoechst 33342 staining and Annexin V-PI double staining. In addition, cell cycle arrest occurred at the G1 phase by CME. Protein kinase B (Akt) plays an important role in cancer cell survival, growth, and division. Akt down-regulates apoptosis-mediated proteins, such as mammalian target of rapamycin (mTOR), p53, and Glycogen Synthase kinase-3β (GSK-3β). CME could regulate the expression levels of p-Akt, p-mTOR, p-GSK-3β, Bcl-2 family members, caspase-3, and PARP. Furthermore, treatment with CME, LY294002 (PI3K/Akt inhibitor), BIO (GSK-3β inhibitor), and Rapamycin (mTOR inhibitor) showed that apoptotic effects occurred through the regulation of the AKT/mTOR/GSK-3β signaling pathway. Our results demonstrated CME could induce apoptosis and cell cycle arrest in HCT116 colon cancer cells.

A Study on the Aspects of Anti-Japanese and Pro-Japanese Literature Shown in Japanese Korean Literature History (일본 한국문학사에 나타난 항일문학과 친일문학 기술양상)

  • Son, Jiyoun
    • Cross-Cultural Studies
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    • v.52
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    • pp.133-164
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    • 2018
  • This purpose of this paper is to focus on anti-Japanese literature and pro-Japanese literature skills among Korean literary history written in Japan, and to observe the differences between Korean and Japanese perception surrounding anti-Japanese and pro-Japanese literature. Analyzed texts are "Taste Korean Literature" by Saegusa Dosikatsu and "The Footsteps of Modern Literature of Chosun" by Shirakawa Yutaka, the earnest modern Korean literary historians written from the perspective of Japanese writers, and though there's no overall written history of literature, they were seen through with the perspective of Omura Masuo, at the forefront of Japanese researchers in modern and contemporary Korean literature. The main results of the review are as follow: First, In Korean literary history by Japan, the frame "pro-Japanese literature" is clearly embedded. It is clearly distinctive from the aspect of China or North Korea, and though it follows the narration system of South Korean literature, it also forms the breaking (turning) point of anti-Japanese and pro-Japanese literature relative to anti-Japanese and pro-Japanese literature. Second, even if it follows the narration system of South Korean literature, that question was constantly raised on existing Korean academic evaluation of anti-Japanese and pro-Japanese literature, and different interpretations of reading were practiced. For example, Korean academic circles highly regard literature of writers such as Kim, Jong han or Lee, Seok hoon, while Korean academics do not place much importance on Lee, Gwang Soo's pro-Japanese elements that are important. The third point is that generous marks are credited to writers with outstanding Japanese or to Japanese creative writing. As a result, they dissolve internal logic in different pro-Japanese collaborators such as Chang, Hyuk Ju, Kim, Sa Ryang, Lee, Seok hoon, or Kim, Yong Jae by melting the same "Japanese literature" in a cage. The last point is reading different inner thoughts of Kim, Jong-han or Lee, Seok-hoon unlike outspoken pro-Japanese collaborators such as Lee, Gwang soo, Jang, Hyuk Joo or Kim, Yong je. These points require more in-depth analysis, and will be continued in follow-up tasks.

A Comparative Study on Buddhist Painting, MokWooDo (牧牛圖: PA Comparative Study on Buddhist Painting, MokWooDo (牧牛圖: Painting of Bull Keeping) and Confucian/Taoist Painting, SipMaDo (十馬圖: Painting of Ten Horses) - Focused on SimBeop (心法: Mind Control Rule) of the Three Schools: Confucianism, Buddhism and Taoism -nd Control Rule) of the Three Schools: Confucianism, Buddhism and Taoism - (불가(佛家) 목우도(牧牛圖)와 유·도(儒·道) 십마도(十馬圖) 비교 연구 - 유불도(儒佛道) 삼가(三家)의 심법(心法)을 중심으로 -)

  • Park, So-Hyun;Lee, Jung-Han
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.40 no.4
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    • pp.67-80
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    • 2022
  • SipWooDo (十牛圖: Painting of Ten Bulls), a Buddhist painting, is a kind of Zen Sect Buddhism painting, which is shown as a mural in many of main halls of Korean Buddhist temples. MokWooDo has been painted since Song Dynasty of China. It paints a cow, a metaphor of mind and a shepherd boy who controls the cow. It comes also with many other types of works such as poetry called GyeSong, HwaWoonSi and etc. That is, it appeared as a pan-cultural phenomenon beyond ideology and nation not limited to Chinese Buddhist ideology of an era. This study, therefore, selects MokWooDo chants that represent Confucianism, Buddhism and Taoism to compare the writing purposes, mind discipline methods and ultimate goals of such chant literatures in order to integrate and comprehend the ideologies of such three schools in the ideologically cultural aspect, which was not fully dealt with in the existing studies. In particular, the study results are: First, the SipWooDo of Buddhist School is classified generally into Bo Myoung's MokWooDo and Kwak Ahm's SimWooDo (尋牛圖: Painting of Searching out a Bull). Zen Sect Buddhism goes toward nirvana through enlightenment. Both MokWooDo and SimWooDo of Buddhist School are the discipline method of JeomSu (漸修: Discipline by Steps). They were made for SuSimJeungDo (修心證道: Enlightenment of Truth by Mind Discipline), which appears different in HwaJe (畫題: Titles on Painting) and GyeSong (偈頌: Poetry Type of Buddhist Chant) between Zen Sect Buddhism and Doctrine Study Based Buddhism, which are different from each other in viewpoints. Second, Bo Myoung's MokWooDo introduces the discipline processes from MiMok (未牧: Before Tamed) to JinGongMyoYu (眞空妙有: True Vacancy is not Separately Existing) of SsangMin (雙泯: the Level where Only Core Image Appears with Every Other Thing Faded out) that lie on the method called BangHalGiYong (棒喝機用: a Way of Using Rod to Scold). On the other side, however, it puts its ultimate goal onto the way to overcome even such core image of SsangMin. Third, Kwak Ahm's SimWooDo shows the discipline processes of JeomSu from SimWoo (尋牛: Searching out a Bull) to IpJeonSuSu (入鄽垂手: Entering into a Place to Exhibit Tools). That is, it puts its ultimate goal onto HwaGwangDongJin (和光同塵: Harmonized with Others not Showing your own Wisdom) where you are going together with ordinary people by going up to the level of 'SangGuBori (上求菩提: Discipline to Go Up to Gain Truth) and HaHwaJungSaeng (下化衆生: Discipline to Go Down to Be with Ordinary People)' through SaGyoIpSeon (捨敎入禪: Entering into Zen Sect Buddhism after Completing a Certain Volume of Doctrine Study), which are working for leading the ordinary people of all to finding out their Buddhist Nature. Fourth, Shimiz Shunryu (清水春流)'s painting YuGaSipMaDo (儒家十馬圖: Painting of Ten Horses of Confucian School) borrowed Bo Myoung's MokWooDo. That is, it borrowed the terms and pictures of Buddhist School. However, it features 'WonBulIpYu (援佛入儒: Enlightenment of Buddhist Nature by Confucianism)', which is based on the process of becoming a greatly wise person through Confucian study to go back to the original good nature. From here, it puts its goal onto becoming a greatly wise person, GunJa who is completely harmonized with truth, through the study of HamYang (涵養: Mind Discipline by Widening Learning and Intelligence) that controls outside mind to make the mind peaceful. Its ultimate goal is in accord with "SangCheonJiJae, MuSeongMuChee (上天之載, 無聲無臭: Heaven Exists in the Sky Upward; It is Difficult to Get the Truth of Nature, which has neither sound nor smell)' words from Zhōngyōng. Fifth, WonMyeongNhoYin (圓明老人)'s painting SangSeungSuJinSamYo (上乘修真三要: Painting of Three Essential Things to Discipline toward Truth) borrowed Bo Myoung's MokWooDo while it consists of totally 13 sheets of picture to preach the painter's will and preference. That is, it features 'WonBulIpDo (援佛入道: Following Buddha to Enter into Truth)' to preach the painter's doctrine of Taoism by borrowing the pictures and poetry type chants of Buddhist School. Taoism aims to become a miraculously powerful Taoist hermit who never dies by Taoist healthcare methods. Therefore, Taoists take the mind discipline called BanHwanSimSeong (返還心性: Returning Back to Original Mind Nature), which makes Taoists go ultimately toward JaGeumSeon (紫金仙) that is the original origin by changing into a saint body that is newly conceived with the vital force of TaeGeuk abandoning the existing mind and body fully. This is a unique feature of Taoism, which puts its ultimate goal onto the way of BeopShinCheongJeong (法身淸淨: Pure and Clean Nature of Buddha) that is in accord with JiDoHoiHong (至道恢弘: Getting to Wide and Big Truth).

A Study on the Peaceful Uses of Outer Space and International Law (우주의 평화적 이용에 관한 국제법 연구)

  • Kim, Han Taek
    • The Korean Journal of Air & Space Law and Policy
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    • v.30 no.1
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    • pp.273-302
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    • 2015
  • The term "peaceful uses of outer space" in the 1967 Outer Space Treaty appears in official government statements and multilateral outer space related treaties. However, the examination of the state practice leads to the conclusion that this term is still without an authoritative definition. As far as the meaning of 'peaceful use' in international law is concerned the same phrases in the UN Charter, the 1963 Treaty of Banning Nuclear Weapons Tests in the Atmosphere in Outer Space and Under Water, the 1956 Statute of IAEA, the 1959 Antarctic Treaty, the 1982 UN Convention on the Law of the Sea, the 1968 Nuclear Non-Proliferation Treaty and the 1972 United Nations Conference of the Human Environment were analysed As far as the meaning of 'peaceful uses of outer space' is concerned the same phrases the 1967 Outer Space Treaty, the 1979 Moon Treaty and the 1977 Convention on the Prohibition of Military or Any Other Hostile Use of Environmental Modification Techniques(ENMOD) were studied. According to Article IV of the 1967 Outer Space treaty, states shall not place in orbit around the earth any objects carrying nuclear weapons or any other kind of weapons of mass destruction, install such weapons on celestial bodies, or station such weapons in outer space in any other manner. The 1979 Agreement Governing the Activities of States on the Moon and Other Celestial Bodies repeats in Article III much of the Outer Space Treaty. This article prohibits the threat or use of force or any other hostile act on the moon and the use of the moon to commit such an act in relation to the earth or to space objects. This adds IN principle nothing to the provisions of the Outer Space Treaty relating to military space activities. The 1977 ENMOD refers to peaceful purposes in the preamble and in Article III. As far as the UN Resolutions are concerned, the 1963 Declaration of Legal Principles Governing the Activities of States in the Exp1oration and Use of Outer Space, the 1992 Principles Relevant to the Use of Nuclear Power Sources in Outer Space(NPS) were studied. And as far the Soft Laws are concerned the 2008 Draft Treaty on the Prevention of the Placement of Weapon in Outer Space and of the Threat or Use of Force against Outer Space Objects(PPWT), the 2002 Hague Code of Conduct against Ballistic Missile Prolifiration(HCoC) and 2012 Draft International Code of Conduct for Outer Space Activities(ICoC) were studied.

Effects of Different Dietary Energy and Protein Levels on Milk Production in Saanen Dairy Goats in Mid Lactation (에너지 및 단백질 공급 수준에 따른 비유 중기 유산양의 유생산성에 미치는 영향)

  • Park, Joong-Kook;Kim, Jong-Duk;Kim, Ji-Ae;Yoo, Jung-Suk;Bae, Gui-Seck;Kim, Chang-Hyun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.30 no.1
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    • pp.35-42
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    • 2010
  • The study was conducted to investigate the effect of dietary energy and protein levels on milk production in Saanen (Capra hircus) dairy goats fed the diets with the different ratio of forage and concentrate. Four lactating goats in mid lactation ($57.5{\pm}10.8\;kg$ BW) were used in a $4{\times}4$ Latin square with 21-d periods. Animals were randomly allocated to one of the four groups; 10.59 MJ ME/kg and 11.90% CP (TJ), 10.14 MJ ME/kg and 12.73% CP (T2), 9.45 MJ ME/kg and 15.19% CP (T3), 8.75 MJ ME/kg and 16.60% CP DM (T4). Also ratio of forage to concentrate was 49: 51 (Tl), 59: 41 (T2), 74: 26 (T3) and 90: 10 (T4). Intakes of forage and concentrate were linearly increased (p<0.05) with their increasing energy levels in the diets. No significant differences were found in total dry matter intake and body weight between treatments. Milk yield in T3 was the highest among treatments (p<0.05). Relative to other treatments, T4 significantly (p

Apolipoprotein E2 & E4 Alleles Influence on the Distribution of the Human Plasma Lipid Profiles in Mormolipidemic Korean Women (아포리포 단백질 E 유전자의 E2와 E4 변이형이 정상 한국여성의 혈중 지질 수준 분포에 미치는 영향)

  • 이명숙
    • Journal of Nutrition and Health
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    • v.29 no.6
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    • pp.642-650
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    • 1996
  • Apo E polymorphism (e2, e3, e4) was among the first reported genetic polymorphism that explained part of the normal variation in plasma cholesterol concentrations. Both alleles E2 and E4 are significantly more frequent in patients with mixed forms of hyperlipidemia and contribute on the observed differences in CHD risk among different populations. Effects of apo E polymorphism on the distribution of plasma lipid profiles were studied in 105 normolipidemic healthy women. The relative frequencies of common alleles for gene locus of apo E in this study were that E3 allele was 0.848, E4 allels was 0.087, and E2 allele was 0.067. SBP and DBP were slightly more elevated in E2 allele than those in E3 and E4. The pulsation was also significantly (p<0.016) increased by E2 allele with excess body fat % in E2 allele. There were no differences in total-, total HDL-, VLDL+LDL-, VLDL- and LDL cholesterol among the apo E alleles. However, apo E2 allele subject had lower level of total HDL and HDL2 cholesterol (P<0.047) and significantly higher lev디 of HDL3 cholesterol (P<0.05) than those in apo E3 and E4 allele subject. The conclusion is that first, it seems that apo E4-mediated alteration through LDL B/E receptors or E receptors in cholesterol metabolism results in lower plasma TG or remanate particles and in higher levels of VLDL+LDL or LDL. Second, apo E2 allele shows reciprocal effects of E4 on the plasma lipid metabolism, respecitvely. Third, apo E2 allele was more atherogenic than apo E4 because the higher levels of HDL3/HDL2 ratio and atherogenic index[(TC-HDL)/HDL]were criticized.

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Comparison of Pakistani and Chinese Ephedra Herba-Containing Gangjihwan in the Improvement Effects of Nonalcoholic Fatty Liver Disease in a High Fat Diet-Fed NAFLD Mouse Model (고지방식이 비만마우스 모델에서 파키스탄산 및 중국산 마황으로 조성된 강지환(降脂丸)의 비알콜성 지방간질환 개선효과 비교)

  • Jo, Ju Heum;Jang, Du Hyon;Jung, Yang Sam;Kim, Jong Hoon;Kim, Byeong Chul;Seok, Hoa Jun;Yoo, Jae Sang;Ku, Ja Ryong;Yoon, Ki Hyeon;Roh, Jong Seong;Ahn, Ye Ji;Lee, Won Kyung;Yoon, Michung;Shin, Soon Shik
    • Herbal Formula Science
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    • v.22 no.1
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    • pp.113-122
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    • 2014
  • Objectives : This study investigated the improvement effects of Pakistani (DF-a) and Chinese Ephedra herba-containing Gangjihwan (DF-b) on nonalcoholic fatty liver disease in a high fat diet-induced obese mouse model. Methods : Eight-week-old C57BL/6N mice were divided into five groups: a normal lean group given a standard diet, an obese control group given a high fat diet, and atorvastatin, DF-a, and DF-b groups given a high fat diet with atorvastatin (10 mg/kg), DF-a (80 mg/kg), and DF-b (80 mg/kg), respectively. After 8 weeks of treatment, body weight gain, blood lipid markers, ALT concentrations, liver weight and histology were examined. Results : 1. Body weight gain was significantly decreased in DF-a, DF-b, and atorvastatin groups compared with control. The extent of decreases was eminent in DF-a group. 2. Circulating concentrations of total cholesterol and LDL-cholesterol were significantly decreased in DF-a, DF-b, and atorvastatin groups compared with control. The decreases were most effective in atorvastatin group. 3. Liver weights were decreased in DF-a, DF-b, and atorvastatin groups compared with control. In particular, liver weight was significantly reduced in DF-b group. 4. Hepatic lipid accumulation was significantly decreased in DF-a, DF-b, and atorvastatin groups compared with control, and the magnitude of which was most effective in DF-b group. 5. Circulating ALT concentrations were decreased in DF-a, DF-b, and atorvastatin groups compared with control, but ALS levels were significantly reduced only in DF-b group. Conclusions : In conclusion, these results suggest that DF-a and DF-b decrease body weight gain, improve blood lipid metabolism, and reduce liver weight and hepatic lipid accumulation, contributing to the improvement of nonalcoholic fatty liver disease. In addition, these effects were similar between Pakistani and Chinese Ephedra herba-containing Gangjihwan.

Study on a pair of small spot of nonsexual gland hind of the larvae in Bombyx mori L. (I) (유충의 생식후원선이 아닌 한 쌍의 소반점에 관한 조사연구 (1))

  • 윤종관
    • Journal of Sericultural and Entomological Science
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    • no.11
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    • pp.51-54
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    • 1970
  • External features of silkworms are not evident during stage of larvae and pupae like in the stage of imagines. In general, therefore, sexing of larvae has been depended on Herold's gland of males or Ishiwata' sexual spots (Anterior sexual spots and posterior sexual spots) of a female 2-3 days after of the 5th age since it is relatively easy to classify in this period. Sometimes. however, we made mistake of sexing since a pair of small spot around the abdomen of 9th abdominal of males and posterior sexual spot of females was found at the same position. This study has been conducted in order to obtain essential points of classifing the above it will be summarized as follows: A. Existence of the males small spots on 9th abdominal segment were varied by races. Through observation races by races the more larvae which had the small spots were found in chinese races than the Japanese, and 57 percent of the Chinese orgin larvae of the small spot were picked among total counted males. B. The small spots were more or less roundly shaped just as posterior sexual spots of females, and these spots were colored slightly even the colors were somewhat different by races. Milky color was seen in the case of the posterior sexual spots. The small spots were observed smaller than the posterior sexual spots. It is difficult classify the sexual spots during stage of mature larvae, but it was easily classified small spots by the naked eye even in the same stage. The small spots were found at the same site of the posterior sexual spots. C. After sexing by based on the small spots and Herold's gland, the sexing through purpae bodies were carried out again to confirm its correctness. Average 5. 2 percent of more opposite sex were found in the latter. This figures supposed to be caused the location of the small spots coincide with the posterior sexual spots. D. The moths, no disorder were found in daily activities and their generative functions depend on the existence of the small spot and thickness of its color. E. It is presumed that existence of the small spots were caused by races, heredity nature, voltinism, moulting, and etc.

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