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Antioxidative Activities by Water-Soluble Extracts of Morus alba and Cudrania tricuspidata (뽕나무(Morus alba)와 꾸지뽕나무(Cudrania tricuspidata)의 수용성 추출물에 의한 항산화 활성)

  • Kim, Hyun-Jung;Cha, Jae-Young;Choi, Myung-Lack;Cho, Young-Su
    • Applied Biological Chemistry
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    • v.43 no.2
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    • pp.148-152
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    • 2000
  • The antioxidative activities of water-soluble extracts from leaves and stem bark of Morus alba and Cudrania tricuspidata were compared in vitro experimental models. Antioxidative activities were measured by inhibition activity against lipid peroxidation of mouse liver microsome, and they were showed in the following order; stem bark of C. tricuspidata(53%)>stem bark of M. alba(43%)>leaves of C. tricuspidata(38%)>leaves of M. alba(43%). In antioxidative activities determined by thiocyanate method and TBA method, the water-soluble extract of stem bark of C. tricuspidata showed the highest antioxidative activity. The water-soluble extracts of leaves were slightly stronger than other extracts in DPPH$({\alpha},{\alpha}'-diphenyl-{\beta}-picrylhydrazyl)$ method. The concentrations of total polyphenolic compound from water-soluble extracts of leaves and stem bark of M. alba and C. tricuspidata were 1.32%, 1.28%, 1.34% and 1.30% respectively. In these results, the water-soluble extract of stem bark from Cudrania tricuspidata showed the highest antioxidative activity.

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Identification and Biosynthetic Pathway of Brassinosteroids in Seedling Shoots of Zea mays L. (옥수수 유식물 신초에서 Brassinosteroid류의 동정 및 생합성 경로 추정)

  • Kang, Min-Wook;Kim, Young-Soo;Kim, Seong-Ki
    • Journal of Plant Biotechnology
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    • v.30 no.4
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    • pp.411-419
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    • 2003
  • The potent biosynthetic precursors, 24$\alpha$-methylcholesterol and 24$\alpha$-methylcholestanol, and the endogenous brassinosteroids (BRs), castasterone (CS) and 6-deoxocastasterone (6-deoxoCS), were identified from shoots of maize seedlings. In addition, the presence for activities of several enzymes involved in the late C6-oxida-lion pathway from 24$\alpha$-methylcholestanol to CS was demonstrated in the plants. However, activity for brassinolide (BL) synthase which catalyze the conversion of CS to BL, the last step of the late C6-oxidation pathway, was not detected in the enzyme solution obtained from the maize shoots. Together with the fact that BL was not identified from the maize shoots, these results strongly suggested that BRs in the maize shoots are biosynthesized during seedling growth and the active BR in the shoots is not BL but CS.

Food Components of Different Parts of Cheonnyuncho (Opuntia humifusa) Harvested from Yeosu, Jeonnam in Korea (전남 여수 돌산지역에서 재배되는 천년초의 부위별 식품성분 분석)

  • Jung, Bok-Mi;Han, Kyung-Ah;Shin, Tai-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.9
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    • pp.1271-1278
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    • 2011
  • This study was carried out to investigate the food components of the fruit, cladodes, and flowers of freezedried Cheonnyuncho harvested from Yeosu, Jeonnam in Korea. The major components of freeze-dried Cheonnyuncho in proximate composition were carbohydrates and crude ash. Ca, K, and Mg were the predominant minerals in Cheonnyuncho. Calcium content was higher in the fruit and cladodes than in the flowers. Two major amino acids, glutamic acid and aspartic acid, made up over 25% of the total amino acids in Cheonnyuncho. Palmitic acid and stearic acid were most abundant out of all the saturated fatty acids in Cheonnyuncho. The saturated fatty acid content of the fruit was higher than that of the flowers and cladodes. The major unsaturated fatty acid of Cheonnyuncho was oleic acid. The cladodes contained unusually high amounts of linoleic acid compared to the fruit and flowers. The major free sugar in the fruit was sucrose, whereas that of the cladodes and flowers was fructose. The total free sugar content was the highest in fruit, followed by cladodes. The most abundant organic acid in the fruits and cladodes was malic acid, while that of the flowers was citric acid. Vitamin A concentration was highest in the flowers whereas vitamin C concentration was highest in the fruit.

Correlation and Combining Ability of Plant Spreading Chracteristics in F1 Hybrids by Diallel Cross in Petunia hybrida (페튜니아 일대 잡종에서 덩굴성 관련형질의 상호관계 및 조합능력)

  • Song, Cheon Young
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.3
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    • pp.179-184
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    • 2009
  • The correlation and combining abilities related with plant spreading characteristics of plant height, plant width, stem length, internode length, number of stem, number of leaves, and number of flower were studied in the $F_1s$ of 10 crosses from the partial five-parent diallel cross in Petunia hybrida. The plant width showed highly positive correlation with internode length, and number of flower. The mean squares of general combining ability(GCA) and specific combining ability(SCA) were highly significant for plant width, number of stem, number of leaves, and number of flowers. The mean square values of GCA were greater than those of SCA for all the characters, showing preponderance of additive gene actions for these characters. The lines of D and I for plant width and length of stem showed relatively high GCA effects. The crosses of $D{\times}I$, $F{\times}I$, and $G{\times}I$ exhibited high SCA effects on plant width, especially $G{\times}I$ effected in internode length and number of flower. The broad sense heritability was generally high compared to narrow sense one. Plant width and stem length, and internode length related to spreading growth showed the higher heritability than the other characters in the broad and narrow sense.

Mesenchymal Stem Cell-derived Exosomes: Applications in Cell-free Therapy (중간엽줄기세포유래 엑소좀: 비세포치료제로서의 활용)

  • Heo, June Seok;Kim, Jinkwan
    • Korean Journal of Clinical Laboratory Science
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    • v.50 no.4
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    • pp.391-398
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    • 2018
  • Mesenchymal stem cells (MSCs) are an attractive resource for refractory patients because of their anti-inflammatory/immunomodulatory capability and multi-lineage differentiation potential. The transplantation of MSCs has led to positive results in preclinical and clinical application to various diseases, including autoimmune disease, cardiovascular disease, cancer, liver cirrhosis, and ischemic stroke. On the other hand, studies have shown that paracrine factors, not direct cell replacement for damaged cells or tissue, are the main contributors in MSC-based therapy. More recently, evidence has indicated that MSC-derived exosomes play crucial roles in regulating the paracrine factors that can mediate tissue regeneration via transferring nucleic acids, proteins, and lipids to the local microenvironment and cell-to-cell communication. The use of these exosomes is likely to be beneficial for the therapeutic application of MSCs because their use can avoid harmful effects, such as tumor formation involved in cell transplantation. Therefore, therapeutic applications using MSC-derived exosomes might be safe and efficient strategies for regenerative medicine and tissue engineering. This review summarizes the recent advances and provides a comprehensive understanding of the role of MSC-derived exosomes as a therapeutic agent.

Experiment of Response with Voltage for Stem Cell Regeneration Treatment (줄기세포재생 치료를 위한 배지의 전압 반응 실험)

  • Yoon, Ki-Cheol;Kim, Jung-Tae;Kim, Beom-Su;Lee, Jun;Lee, Jong-Chul
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.41 no.7
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    • pp.809-815
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    • 2016
  • In this paper, the electrical values of resistance, current, and voltage condition of reactivity is analyzed by applying the direct current (DC) voltage in medium for stem cell regeneration treatment. The voltage response by medium is related to electrical stimulation in the process of induction of differentiation for stem cell and the differentiation condition can be checked depending on the response of voltage condition. If the voltage level is lower in reacting response of a medium, the stem cell stimulation condition is stable, and if the voltage changing level is higher, the stem cell stimulation condition is unstable and a considerable loss will be resulted in the differentiation process. In this research, the optimization of electrical stimulation condition is expected for possible stem cell regeneration treatment.

Shoot Regeneration of Mature Nodal Segments in Poplar (Populus deltoides) (미류나무 성숙 절간조직으로부터 식물체 증식)

  • Yang Hee-Sun;Kang Hoduck
    • Korean Journal of Plant Resources
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    • v.18 no.3
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    • pp.403-409
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    • 2005
  • Mature nodal segments of 2-year-old greenhouse stock plant were cultured on Murashige and Skoog basal medium supplemented with the different kinds and various concentrations of cytokinins to produce multiple shoots in in vitro condition. The most adventitious shoots were produced from excised ends of nodal segments. The highest average number $(24.6\;{\pm}\;4.6)$ of shoots was produced with the combination of BA 1.0mg/L and TDZ 0.1mg/L in MS medium. In addition, several shoots were formed from lenticels of bark cambium with the same treatment. These concentrations promoted high shooting capability upto $94.6\%$ and NAA was the best cytokinin among five different PGR sources.

An Anatomical Comparison of the Secondary Xylem in the Branch, Stem and Root of Salix glandulosa and Quercus variavilis (왕버들과 굴참나무의 뿌리, 줄기 및 가지에 있어서 이기목부(二期木部)의 해부학적(解剖學的) 비교(比較))

  • Ryu, Ha Yong;Soh, Woong Young
    • Journal of Korean Society of Forest Science
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    • v.77 no.3
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    • pp.283-293
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    • 1988
  • Anatomical features of the secondary xylem were described in the branches, stems, and roots of Salix glandulosa and Quercus variavilis. In Quercus variavilis, the stem shows ring-porous wood, while the root shows diffuse-porous wood. In both species, it is found that the boundaries between the growth rings are not well defined in the roots. Both vessel elements and fibers are the longest in the roots followed by the sterns and the branches in turn and diameter of vessel elements shows the same trend. In cross section, the number of vessels per unit area are the longest in the branches followed by the stems and the roots in turn as well. Perforation plates are simple in both species and their angles are wide in the root, intermediate in the stems, and narrow in the branches. In tangential section, height of rays is taller in the roots than in the stem and branches.

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Isolation of main component and antioxidant activities on the stem and root of Rosa rugosa (해당화 줄기 및 뿌리의 주성분 분리 및 항산화활성)

  • Park, byoung-Jae
    • Korean Journal of Plant Resources
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    • v.21 no.5
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    • pp.402-407
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    • 2008
  • Total phenol content was obtained from the stem extract of 7.8 (g/100g, D.W.) and root extract of 10.5 (g/100g, D.W.) on Rosa rugosa. S-4 and R-2 compounds were isolated from the stem and root of R. rugosa. The structure of S-4 and R-2 compounds was assigned as catechin by $^{1}H,\;^{13}C$ CNMR signal and TOF-MS. The tannin content of stem extract was 752.5 (mg/100g, D.W.) as 6.3 of gallic acid, 61.5 of epicatechin and 684.8 of catechin. The tannin content of root extract was higher about 2 times (1676.0) as 6.8 of gallic acid, 160.3 of epicatechin and 1508.5 of catechin than that of stem. It was shown that catechin was main compound in the stem and root extracts of R. rugosa. Antioxidant activities were stronger low concentration, and were not different of stem and root extracts. As the results, stem and root of R. rugosa were evaluated, as natural antioxidant resource.

Co-author network for convergent research pattern analysis in stem cell sector (줄기세포분야 융합연구형태 분석을 위한 공저자 네트워크)

  • Jang, Hae-Lan
    • Journal of the Korea Convergence Society
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    • v.8 no.9
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    • pp.199-209
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    • 2017
  • This study was carried out to confirm a convergent research pattern and researchers' role in stem cell sector by social network analysis. Articles were extracted from 1996 to 2012 in PubMed, 515 authors of 270 embryonic stem cell and induced pluripotent stem cell articles and 1,515 authors of 580 adult stem cell and mesenchymal stem cell articles. Degree(D) and betweenness(B) centrality was measured and co-author network was generated for researcher's role. As a result, Core researcher and Intermediary researcher was identified in co-author network. Core researcher had high D. centrality, otherwise high B. centrality or not. Intermediary researcher for convergent research had high B. centrality and low D. centrality. Conclusively, co-author network will be used as objective data not only to find core researchers in subject area for improving achievement but also to select experts for research project evaluation.