• The Science & Technology
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• s.507
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• pp.23-28
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• 2011

• Journal of Korean Society of Forest Science
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• v.90 no.2
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• pp.184-189
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• 2001

Multiple shoots were induced from in vitro shoot originated from winter bud of P. yedoensis from Jeju. Most explants grow in similar type among the five different media but affected by supplement of sucrose regardless of media. For mass-propagation various concentrations of BAP or $GA_3$ were treated in the medium respectively. BAP was very effective to produce multiple shoots and 3.5~9.5 shoots were formed on the explant. The shoots induced on the high levels of BAP have short internodes. No shoots were induced on the treatment of $GA_3$ but roots were induced on it. When $GA_3$ was supplemented with the medium containing BAP, multiple shoots were produced from the explants. The medium(WPM) containing with $0.5mg/{\ell}$ BAP and $4.0mg/{\ell}$ $GA_3$ was most effective to produce multiple shoots. When the explants were cultured for 8 weeks, 39.5 shoots were developed in average.

• Clinical and Experimental Reproductive Medicine
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• v.37 no.2
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• pp.99-113
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• 2010

Objective: It has been recently reported that very small stem cells with pluripotency are detected in murine and human. The purposes of this study are to confirm whether very small putative stem cells (VSPSCs), which have the proper characteristics of stem cells as well as the expression of stem cell markers, are detected in human endometrium. Methods: The endometrial cells of 5 women, which were obtained by endometrial biopsy, were cultured for 2 weeks and were confirmed for the expressions of alkaline phosphatase, OCT-4 and CXCR4 by immunochemistry. Subsequently VSPSCs were separated by percoll density gradient method and were cultured. Also VSPSCs and their derived cells were confirmed for the expressions of OCT-4 and CXCR4. Results: The colonies, which is composed with VSPSCs less than 3 ${\mu}m$ and the 5~15 ${\mu}m$ sized hyperchromatic round cells, were detected in the endometrium of all of women and showed the strong expressions of alkaline phosphatase, OCT-4 and CXCR4. In culture after the separation of VSPSCs by percoll, these cells showed the morphological and functional characteristics of stem cells; self-renewal, colony formation, embryoid body-like formation and differential plasticity. VSPSCs formed gradually the 5~15 ${\mu}m$ sized hyperchromatic round cells and the 10~20 ${\mu}m$ sized sphere-shaped cells by cell-to-cell aggregation or cell fusion. Then these cells differentiated the various cells including fibroblast-like cells, nerve-like cells and endothelium-like cells. VSPSCs and their derived cells often showed the strong expressions of OCT-4 and CXCR4. Conclusion: VSPSCs less than 3 ${\mu}m$ and their derived cells are detected in human endometrium and these cells have the proper characteristics of stem cells and the expressions of stem cell markers as alkaline phosphatase, OCT-4 and CXCR4.

• Journal of Science and Technology Studies
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• v.15 no.1
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• pp.181-214
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• 2015

This study analyzed the characteristics of the politics of technoscience and governance in South Korea, taking advantage of the policy changes on the stem cell research after Hwang's affair. In spite of generally accepted conventional wisdom that stem cell research had been suffering 'crisis' after the Hwang's affair, South Korea succeeded in developing the first and the largest stem cell product in the world. However, considering the fact that the stem cell research capabilities and technological competitiveness of Korea have been assessed as relatively low compared to the development performance, there is a need to extrapolate how such result could be achieved. To answer these questions, we analyzed changes in the R&D expenditure before and after the scandal and verified the 'crisis of stem cell research' following the reduction of financial support from government. From the analysis of literature on the policy reports and news, we described the process of discourse changes in policy and analyzed the characteristics of the politics of technoscience and governance of stem cell research. This study emphasized that the government R&D and regulation policy play the key roles in the development of stem cell research rather than in the technological competitiveness in South Korea. Furthermore, this study argued that democratic governance still does not work under the policy conditions that technocratic decision-making of stem cell research fails to learn from the Hwang's affairs.

• Journal of the KSME
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• v.50 no.11
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• pp.35-38
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• 2010

이 글에서는 줄기세포 연구의 중요성과 마이크로 미세유체칩에서 줄기세포의 거동을 연구한 선행 연구 사례들을 소개하고자한다.

• The Korean Society of Law and Medicine
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• v.16 no.1
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• pp.191-219
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• 2015

The Japanese government has announced that it would invest 30 billion yen in iPS cell research for the next 10 year, and the Japan National Assembly has made an act that supports the iPS cell research. This study analyzes 1) the current trend and application field of stem cell research under Japan; 2) recent policy and regulation change in stem cell research and its application under Japan. This treatise reviews about "Act for Promoting Regenerative Medicine", "Act for Assuring Safety of Regenerative Medicine", and "the Revision of Pharmaceutical Act under Japan. This study may serve as the fundamental reference for the improvements of legal and institutional systems with regard to regulations on the stem cell research under Korea.

• Development and Reproduction
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• v.13 no.4
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• pp.271-280
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• 2009

Human placenta is abundant source of adult stem cells. Especially, amniotic epithelial cells have stem cell characteristics, expressing surface markers normally present on embryonic stem cells and germ cells. However, culturing and expanding amniotic epithelial cells in vitro without feeder cells are difficult due to endogenous characteristics of epithelial cells. In the present study, amniotic epithelial cells are isolated and proliferated in several passages by applying dithiothreitol and a Rho-associated kinase inhibitor in culture media. The cultured amniotic epithelial cells showed the epithelial and stem cell characteristics. In conclusion, human placenta-derived amniotic epithelial stem cells can be a major source of stem cells for medical treatment of various diseases without any controversial issues.

• Journal of Dental Rehabilitation and Applied Science
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• v.25 no.2
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• pp.191-200
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• 2009

Dental Pulp Stem Cells are superior to other types of adult stem cell. Because of teeth are easy to access and are extracted throughout life. A supernumerary tooth is an important clinical problem found in various populations of the world. The incidence of supernumerary teeth varies depending on the literature source. Pediatric dentists are routinely extracted them. However, no studies have been reported regarding Dental Pulp stem cells in a supernumerary tooth, and we failed to note that a valuable source of human stem cell. Herein, we tried to show that a supernumerary tooth contains cells that display the characteristic features of stem cells.

• Journal of Convergence for Information Technology
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• v.8 no.2
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• pp.61-65
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• 2018

Our experiment studied that grafted stem cells reduced behavioral deficiency in rodent animal models of clip compressive surgery inducing spinal cord infarction. Our research proved the effect of embryonic stem cells to the spinal cord infarction caused by compressing T9-10 with an aneurysm clip, focusing the application of grafted stem cells for reduction of infarction and regeneration of spinal cord nervous injury. Therefore, our research suggests manifest results that implantation of mouse embryonic stem cell could show behavioral improvement after severe spinal cord damage. Therefore, mouse embryonic stem cell (mESC) could be useful application for the method in neurological injury. Conclusively, stem cell implant therapy may enhance the effectiveness of stem cell implant for central nervous system injury.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.137-137
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• 2019

돌출줄눈은 산지하천의 만곡부의 빠른 유속을 감소시키기 위하여 활용된다. 본 연구에서는 친환경 디자인의 나무줄기 돌출줄눈(Tree Trunk Rip, TTR)과 사다리꼴 돌출줄눈(Trapezoid Rip, TR)의 흐름저항을 비교 분석하기 위하여 개수로 수리실험을 수행하였다. 실험은 길이 9m, 폭이 0.6m이며 경사가 0.0035로 고정된 개수로의 한쪽 측벽에 돌출줄눈을 설치하여 진행하였다. 사다리 꼴 돌출줄눈의 형상은 밑변 각이 $63^{\circ}$이며 무차원 설치간격 ${\lambda}_{nv}$가 6, 9, 12인 경우이다. 나무줄기 돌출줄눈의 기본 형상은 사다리꼴이고 표면은 나무껍질 무늬이며 ${\lambda}_{nv}$가 약 10이다. 나무줄기 돌출줄눈의 간격은 사다리꼴 돌출줄눈의 최적 설치 간격 9~12배 범위에 해당되고 평균 마찰계수는 사다리꼴 돌출줄눈의 9~12배의 평균 마찰계수 범위에 포함되었다. 사다리꼴 돌출줄눈의 ${\lambda}_{nv}$가 9, 12일 때의 전체 저항에 대한 형상저항의 비는 평균 $69.4{\pm}5.8%$였으며 나무줄기 돌출줄눈은 $70.2{\pm}2.1%$로 사다리꼴 돌출줄눈과 유사하다. 산지하천 흐름저항을 위한 돌출줄눈 설치에 있어 친환경적 디자인을 고려한 나무줄기가 사다리꼴 돌출줄눈보다 활용도가 클 것으로 기대된다.