• Title/Summary/Keyword: 주사현미경

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THE ANALYSIS OF REMINERALIZATION EFFECT IN FLUORIDE VARNISH USING CONFOCAL LASER SCANNING MICROSCOPE (공초점 레이저 주사 현미경을 이용한 불소 바니쉬 재광화 효과의 분석)

  • Kwon, Ji-Hoon;Park, Ho-Won;Lee, Ju-Hyun;Seo, Hyun-Woo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.35 no.1
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    • pp.57-64
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    • 2008
  • It is well established that fluoride products play an important role in the prevention and remineralization of carious lesion. Fluoride varnish is a concentrated topical fluoride and varnishes adhere to tooth surface, permitting prolonged fluoride exposure and uptake. In this study, the artificial initial enamel caries was caused on the sound human enamel and divided 60 specimens into three groups. Group 1 and group 2 were treated with the topical application of fluoride varnish and stored in artificial saliva for 1 and 2 weeks. Group 3 was stored in artificial saliva for 2 weeks, which acted as control group. Changes in mineral contents were analysed with the confocal laser scanning microscope. The following results were obtained: 1. In group 1 and group 2, the total fluorescence of the lesion(TFL) was reduced in remineralized area compared to in demineralized area(p<0.05). 2. The total fluorescence of the lesion of remineralized area was more reduced in group 2 than in group 1(p<0.05). 3. The total fluorescence of the lesion was more reduced in group 2 than in control group(p<0.05). 4. Confocal laser scanning microscope can be used in quantitative analysis of remineralization by fluoride varnish.

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The Effects of Natural Disinfectants Naringin on Corneal Epithelium and Endothelium of Rabbit - By Scanning Electron Microscopy (천연보존제 나린진이 가토안의 각막 상피와 내피에 미치는 영향 - 주사전자현미경을 통한 고찰)

  • Kim, In-Suk;Yoo, Geun-Chang;Chae, Soo-Chul;Jeon, Chang-Jin
    • Journal of Korean Ophthalmic Optics Society
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    • v.10 no.1
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    • pp.63-69
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    • 2005
  • The effects of R products of B company and natural disinfectant naringin, well-known chemical disinfectant on Corneal endothelium and epithelium of rabbit were observed by scanning electron microscope. The main component of naringin is extract of grapefruit seed, which is one of the flavonoid widely recognized as natural antioxidants and used as preservative agent of food and cosmetics. The chemical disinfectants cytotoxicity in cell cultures were announced by MIT assay and LDH leakage assay. But there has been no study about that chemical disinfectants and natural disinfectants is inserted drops on rabbit's cornea directly. In this test R products B company and natural disinfectants has been dropped on the cornea of rabbits for one weeks and observed cytotoxiciy by light camera after rose bengal staining. Also, we analysed the damage of corneal epithelium and endothelium morphologically after enucleating the cornea by scanning electron microscope.

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IN VITRO STUDY ON APICAL SEAL AND SEM MORPHOLOGY IN CANALS FILLED WITH RESIN-BASED ROOT CANAL SEALER AND SELF-ETCHING PRIMER (SELF-ETCHING PRIMER와 레진계 근관 SEALER 사용시 근관벽의 형태와 치근단 근관밀폐에 대한 실험적 연구)

  • Kang, Sam-Hee;Park, Dong-Sung;You, Heyon-Mee;Oh, Tae-Seok
    • Restorative Dentistry and Endodontics
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    • v.25 no.4
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    • pp.543-549
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    • 2000
  • Gutta-percha와 근관sealer를 사용한 근관 충전은 가장 많이 이용되고 성공률이 높은 방법이지만, 현재 사용되는 모든 근관sealer는 미세누출을 나타낸다. 따라서 더 효과적인 근관 폐쇄를 이루기 위한 재료 및 방법들이 연구되어왔다. 이중 상아질 접착제는 근관 충전재와 함께 사용되어 미세누출을 감소시키지만, 근관 내에 사용하기에 술식이 복잡하고 기술이 요구된다. 본 연구의 목적은 gutta-percha와 레진계 근관 sealer로 충전한 근관에서 self-etching primer를 미리 도포한 경우와 도포하지 않은 경우의 치근단 미세누출을 비교하고, self-etching primer를 도포함에 있어 근관 내에 적용하는 방법에 따른 미세누출을 비교하는 것이다. 또한 근관sealer와 상아질 계면을 주사전자현미경으로 관찰하여, 이 결과를 미세누출과 관련시켜보고자 하였다. 36개의 발거된 사람의 단근치에서 치관부를 절단, 제거하고 ProFile로 근관 형성한 후, 무작위로 선택하여 4개의 군으로 분류하였다. 1군에서는 주사기와 30게이지 주사 바늘로 self-etching primer를 근관 내에 적용하였고, 2군에서는 self-etching primer를 paper point에 적셔 근관 내에 적용하였다. 3군에서는 self-etching primer를 적용하지 않았다. 1, 2, 3군의 치아를 gutta-percha와 AH26 sealer를 사용하여 continuous wave 충전법으로 충전한 후 치근단공 주위 3mm를 제외한 치근변에 nail polish를 2회 도포하였고, 4군(음성 대조군)은 치근면 전체에 도포하였다. 1군과 2군에서 각각 2개의 치아는 주사전자현미경적 관찰을 위해 준비하였다. 모든 치아를 Methylene Blue 수용액에 48시간동안 침적, 수세한 후 치아 장축에 평행하게 양분하여 10배의 실물확대현미경 하에서 치근단 색소 침투를 관찰하였다. Self-etching primer를 도포한 군과 도포하지 않은 군 사이에는 평균 미세누출량에 유의한 차이가 없었다. 주사 바늘로 적용한 군과 paper point로 적용한 군 사이에도 평균 미세누출량에 유의한 차이가 없었다. 상아질과 근관 sealer계면의 주사전자현미경 관찰 결과 일부분에서 긴밀한 접착 관계를 나타내었고, 다른 부분에서는 간극을 나타내었다.

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Two Unrecorded Speces of Hemicycliophora minora and Pratylenchoides ivanovae and Additional SEM Description of Pratylenchoides utahensis and Rotylenchus feroxcis from Korea (한국 미기록 선충 Hemicycliophora minora, Pratylenchoides ivanovae 2종 보고 및 Pratylenchoides utahensis와 Rotylenchus feroxcis의 주사전자현미경 관찰)

  • Kim Dong-Geun;Lee Joong-Hwan;Kim Seung-Han;Choi Jae-Sin
    • Korean journal of applied entomology
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    • v.44 no.1 s.138
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    • pp.5-11
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    • 2005
  • Hemicycliophora minora Wu, 1966 and Pratylenchoides ivanovae Ryss, 1980 are newly collected from Korea. Five species of Hemicycliophora and 3 species of Pratylenchoides were reported in Korea and key is given. Two preciously described species, Pratylenchoides utahenis Baldwin, Luc & Bell and Rotylenchus feroxcis Eroshenko were observed under the scanning electron microscope.

A Flexure Guided Planar Scanner for Scanning Probe Microscope ; Part 2. Evaluation of Static and Dynamic Properties (주사 현미경용 평면 스캐너 Part 2 : 정 · 동 특성 평가)

  • Lee, Moo-Yeon;Gweon, Dae-Gab;Lee, Dong-Yeon
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.15 no.11 s.104
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    • pp.1295-1302
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    • 2005
  • This paper shows experimental evaluation results of the nano-positioning planar scanner used in the scanning probe microscope. The planar scanner is composed of flexure guides, piezoelectric actuators and feedback sensors as like explained in detail in Ref. (5). First, the fabrication methods were explained. Second, as the static Properties of the Planar scanner. we evaluated the maximum travel range & crosstalk. Also, we presented the correcting method for crosstalk using electric circuits finally. as the dynamic properties of the planar scanner, we evaluated the first resonant frequency. Also, we presented the actual AFM(atomic force microscope) imaging results with up to 2Hz imaging scan rate. Experimental results show that properties of the proposed planar scanner are well enough to be used in SPM applications like AFM.

Comparison of Skin Condition Before and After Use of Scrub Cosmetics and Microscopic Characteristics of Microbeads (스크럽 화장품의 사용 전과 후의 피부상태 비교와 이에 첨가된 마이크로비드의 현미경적 특성)

  • Kim, Hoon;Chang, Byung-Soo
    • Journal of Convergence for Information Technology
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    • v.9 no.6
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    • pp.211-217
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    • 2019
  • In this study, the change of skin condition was analyzed by skin analysis equipment using scrub cosmetics, and the morphological characteristics of microbead were observed by dermascope and scanning electron microscope. In the dermascope observation, during the process of cleansing, the microbeads attached to the skin existed in close contact with each other or dispersed. The skin after scrubbing was clean and smooth and the fine wrinkles between epidermal keratinocytes were reduced. In the scanning electron microscopic observation, the microbead surface did not have severe bending or rough surface. The skin moisture and oil content were higher than the scrub skin before the scrub, and there was no significant difference in the pH.