• Korean Journal of Poultry Science
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• v.35 no.1
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• pp.71-78
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• 2008

This study was conducted to investigate the effects of dietary supplementation of herbs and plant extracts (PE) on the performance, small intestinal microflora and immune response in laying hens. A total of 1,440 Hy-Line Brown laying hens of 67 wks old were assigned to one of the following 9 dietary treatments : T1 : Control (C), T2 : C + Avilamycine 6 ppm, T3 : C + Herb $Mix^{(R)}$ 0.2%, T4 : C + Biostrong $510^{(R)}$ 0.02%, T5 : C + $APEX^{(R)}$ 0.02%, T6 : C + $Digestarom^{(R)}$ 0.02%, T7 : C + $Phellozyme^{(R)}$ 0.1%, T8 : C + $Galicin^{(R)}$ 0.05%, T9 : C + CRINA $Poultry^{(R)}$ 0.05%. Each treatment was replicated 8 times with twenty birds housed in 2 bird cages. Twenty bird units were arranged according to completely randomized block design. Feeding trial lasted 6 wks under 16 hours lighting regimen. Hen-day egg production was not significantly different among the treatments, but that of supplemented groups tended to be higher than the control. There were significant differences among treatments in feed intake and feed conversion ratio. Feed intake was higher in the supplemented groups than the control. Feed conversion ratio was higher in T8 than other treatments. Egg shell color index and egg yolk color index were significantly different among treatments. Egg shell color was affected by supplements and egg yolk color index of T9 (PE-CRINA) was significantly higher than the control. Haugh unit was not significantly different among treatments. There were significant differences in leukocytes and erythrocytes parameters. The level of serum WBC and stress index (heterophil/lymphocyte) were higher in supplemented groups than the control. The level of RBC tended to be lower in the herb or PE groups than the control. The concentration of serum IgG was not significantly different among the treatments, but all those of the supplemented groups were higher than the control. The number of Lactobacilli spp. tended to increase and that of Cl. perfrigens decrease in the supplemented groups. The number of E. coli significantly decreased in the supplemented groups. The results of this experiment showed that feeding herbs and plant extracts to laying hens tended to improve the egg production and affect positively on the level of blood parameters and small intestinal microflora.

• Korean Journal of Poultry Science
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• v.43 no.4
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• pp.197-206
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• 2016

This work was carried out to investigate effects of the freezing/thawing method on duck meat kept in a freezer for a month. The meats used were breast muscle collected from Korean native ducks (KND) that were fed for 8 weeks (2.8 kg of live weight). Forty-five samples were used after being frozen in storage for one month and were then divided into 5 treatments (3 replications/treatment, 3 samples/replication). Five treatments (CON, FFFT, FFST, SFFT and SFST) were control groups (CON) and four were experimental groups, using $2{\times}2$ complex factors with two freezing methods (fast freezing, FF, $-50^{\circ}C$ in a deep freezer; slow freezing, SF, $-20^{\circ}C$ in a common freezer) and two thawing methods (fast thawing, FT, 5 h $12^{\circ}C$ with flow water; slow thawing, ST, 24 h $5^{\circ}C$ in a refrigerator). Lightness of KND meat in FF and FT groups was lower than that of control (P<0.05). Yellowness of KND meat of the ST group was higher than that of control (P<0.05). Cooking loss (CL) and water holding capacity (WHC) of KND meat in the control were lower than those of the freezing and thawing groups (P<0.01, P<0.05), but shear force (SF) of the control was higher than that of other groups (P<0.01). Moisture content of the ST group was higher than that of the FT group (P<0.05), and protein content of the FF group was higher than that of control (P<0.05). Stearic acid (C18:0) of the SF group was higher than that of the FF group (P<0.05). Arachidonic acid (C20:4n6) of control was higher than that of the SF and ST groups (P<0.01, P<0.05). Alanine, aspartic acid, glutamic acid, serine, and tyrosine content of the control were lower than that of the freezing and thawing groups (P<0.05). These results show that freezing and thawing methods affect meat color, shear force, cooking loss, and WHC-related water content.

• Korean Journal of Poultry Science
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• v.41 no.2
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• pp.135-142
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• 2014

This study was conducted to evaluate dietary effects of Marigold extract on laying performance, egg quality, oxidative stability of egg yolk and lutein transfer into chicken eggs. A total of one-hundred eighty nine 55-wk-old Hy-Line Brown layers were divided into seven groups and fed control diet or each experimental diet containing 0.1, 0.3, 0.5, 1.0, 1.5 or 2.0% Marigold extract. Egg production, egg weight and daily egg mass were not affected by dietary treatments. The yolk colors in groups fed diets containing Marigold extract were significantly higher than that of control. The Haugh unit were tended to be improved by feeding of diets containing Marigold extract although there were no significant difference in egg shell strength and thickness. The MDA (malondialdehyde) contents in groups fed diets containing Marigold extract above 0.5% were significantly reduced than that of control. After 14d of storage, the Haugh unit values in groups feed diets containing 0.3 and 1.0% Marigold extract were significantly higher than that of control (p<0.05). The concentration of lutein in egg yolk increased by feeding of Marigold extract. When 2% Marigold extract was supplemented to the diet, lutein content of egg was increased as much as 1.71 mg/60 g. These results indicated that the use of Marigold extract in layer diets was effective in egg quality and for the production of lutein fortified eggs.

• Journal of Animal Science and Technology
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• v.52 no.5
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• pp.389-398
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• 2010

The objective of this study was to investigate the effects of three strains of Bacillus subtilis (B. subtilis) supplemented to diets on egg production, egg quality, egg yolk cholesterol levels, the profile of cecal microflora, and tibia characteristics in laying hens. One hundred sixty 76-week-old Hy-Line Brown layers were randomly divided into 4 groups with 4 replicates per group (10 birds per replicate). Birds in the control group were fed a corn-soybean meal based diet. The remaining three treated groups were fed the control diet containing either 0.05% B. subtilis Ch3 (T1), 0.05% B. subtilis Ch3 + B. subtilis W1 (T2) or 0.05% B. subtilis commercial product (T3) for 6 weeks, respectively. There were no differences in feed intake, egg weight, egg production and egg mass among the groups. The dietary supplementation of B. subtilis improved eggshell strength and Haugh units compared to those of control (P<0.05). The activities of GOT and GPT in serum were not also affected by the dietary treatments. The population of total microbes and lactic acid bacteria in cecum were significantly increased by the dietary B. subtilis (P<0.05), but not the coliforms. The cholesterol concentration in egg yolk and serum in the treated groups were significantly decreased compared to those of control (P<0.05). Also, The levels of phospholipids in serum were significantly decreased compared to those of control (P<0.05). The supplementation of three strains of B. subtilis to diets significantly increased the contents of tibia ash compared to that of control (P<0.05). Thus, this study showed significant improvements in egg quality, such as eggshell strength and Haugh unit, by dietary B. subtilis strains. The B. subtilis strains added to the diets modulated the profiles of cecal microflora, reflecting beneficial effects in laying hens.

• Korean Journal of Poultry Science
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• v.35 no.2
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• pp.183-190
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• 2008

This study was conducted to investigate the effect of feeding diets supplemented with ${\beta}-glucan$ products on the performance, small intestinal microflora and immune response in laying hens. The ${\beta}-glucan$ products used in the experiment were $BetaPolo^{(R)}$ ; soluble ${\beta}-glucan$ of microbial cell wall origin, $HiGlu^{(R)}$ ; microbial cell wall origin, $OGlu^{(R)}$ ; oat origin, $BGlu^{(R)}$ ; barley origin. A total of 720 Hy-Line Brown laying hens of 40wks old were divided into 5 dietary treatments : T1 ; Control( C), T2 ; $BetaPolo^{(R)}$, T3 ; $HiGlu^{(R)}$, T4 ; $OGlu^{(R)}$, T5 ; $BGlu^{(R)}$. Each treatment was replicated 4 times with 36 birds/replicate housed in 2 bird cages, and arranged according to completely randomized block design. Feeding trial lasted 40ds under 16 h lighting regimens. There were significant differences among treatments in hen-house egg production feed intake and feed conversion. HiGlu treatment was significantly higher than OGlu treatments in hen-house egg production. ${\beta}-glucan$ supplemented treatments were lower than the control in feed intake and feed conversion ratio. All ${\beta}-glucan$ supplemented treatments were significantly higher than the control in eggshell strength. Eggshell color and Haugh unit tended to be lower in the supplemented group than the control. IgY concentration was not significantly affected by treatments. At $5^{th}$ week of experiment, however, IgY concentration tended to increase in the supplemented groups. Among the leucocytes parameters, WBC, heterophil, lymphocytes, monocyte and eosinophil concentration were lower in the supplemented groups than those of the control. Among erythrocytes, HCT(hematocrit) and MCV(mean corpuscular volume) were significantly affected by treatment. MCV of supplemented groups were higher than that of the control. Immunoglobulin concentrations in the birds were not significantly different among treatments. However, IgA concentration tended to be low in the supplemented groups than the control. The cfu of small intestinal microflora were not significantly different among treatments, but that of Cl. perfringens tended to be lower than the control. The result of this experiment indicateted that feeding ${\beta}-glucan$ to laying hens improve feed conversion ratio and eggshell strength. Also intestinal microflora and immune responses are modified.

• Korean Journal of Poultry Science
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• v.34 no.4
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• pp.245-251
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• 2007

This study was conducted to investigate the effects of modification of a herbal recipe(Herb $Mix^{(R)}$) on the growth of pullet and laying performance of hens. The formula of Herb $Mix^{(R)}$, a mixture of Rehmannia glutinosa, Angelica gigas, Discorea japonica, Glycyrrhiza uralensis, Schisandra chinensis and Ligusticum jeholense, was modified in mixing ratio. A total of 1,120 pullets(Hy-Line Brown) of 14 wks old were assigned to seven treatments; control, Herb $Mix^{(R)}$(HM), R. glutinosa fortified HM, A. gigas fortified HM, D. japonica fortified HM, G. uralensis fortified HM, S. chinensis fortified HM, L. jeholense fortified HM and Flavomycin supplemented diet. Each treatment had 8 replicates of 20 birds each housed in 2 birds cages. Body weight at 10% egg production was significantly(P<0.05) influenced by treatments. Birds fed A. gigas fortified HM diet were heaviest followed by L. jeholense fortified HM, HM-original and D. japonica fortified HM, Flavomycin supplemented diet and R. glutinosa while those fed control diet were lightest. Also, age reaching 50% egg production and peak production was earliest in A. gigas fortified HM and latest in the control. Egg production, feed intake, feed conversion and egg weight were significantly influenced by treatments. Significant improvement in egg production and feed intake was shown in A. gigas fortified HM treatment. Feed conversion ratio was lowest in antibiotic(Flavomycin) treatment and egg weight was heaviest in L. jeholense fortified HM treatment. There were no significant differences among treatments in intestinal microflora but cfu of Cl. perfringnes and E. coli tended to be lower in HM treatments than the control. Among the leucocytes of blood, the HM treatments were lower than the control in counts of white blood cell and heterophils. It was concluded that modification of Herb $Mix^{(R)}$ fortifying with A. gigas, D. japonica and L. jeholense significantly influence growth and laying performance of birds.

• Korean Journal of Poultry Science
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• v.32 no.1
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• pp.15-21
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• 2005

This Study was conducted to investigate the effects of microbial phytase in low phosphirus and calcium level diet on the performance and nutrient digestibility in laying hens. One hundred ninety two, 50 wks old, ISA brown commerical layers were used for 12 weeks feeding trial after 7-d adjustment period. Four dietary treatments included CON(control; Co.), P2 ($0.06\%$ Natuphos, BASF) and P3 ($0.06\%$ PHOSMAX, GENOFOCUS). Ca and available P concentrations of P1, P2 and P3 were 90 and $50\%$ of NRC recommecdations to accentuate difference in response to phytase availability. In whole period, egg production was not affected by treatments. At 12 weeks, egg weight was significantly increased in adding phytase treatments (P<0.05). Egg shell thickness was increased in P1, P2 and P3 treatments compared with control (P<0.05) at 9 weeks. Ca concentration of serum tended to decrease in P1 treatment without significant difference (P>0.05). Ca and P concentrations of tibia were higher in layers fed dietary phyrase than those fed control diet without significant difference (P>0.05). Digestibilities of DM, N and ash were improved in P1 treatment compared with P2 and P3 treatments (P<0.05). Ca and P digestibilities were the highest in P2 treatment (P>0.05), but was not significant difference between control and P1 treatments.

• Korean Journal of Poultry Science
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• v.16 no.3
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• pp.149-156
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• 1989

Two broiler experiments were carried out to determine whether supplementation of zeolite in the diet would affect broiler performance and economical efficiency. Day old male broiler chickens of Abor Acres strain were used. In the first experiment,60 birds were distributed into f groups of 10 birds each. Three groups of chickens were randomly placed on one of the following single-stage (0-6wks) experimental diets； control diet (non-supplemented) or 2% zeolite supplemented diet (supplemented to the control). In the second experiment,216 birds were distributed into 24 groups of 9 birds each. Eight groups of chickens were randomly placed on one of the 3 two-stage diet (starter；0-4wks, finisher；5-7wks)；control diet ($T_1$ , non-supplemented), 2% zeolite diet ($T_2$：isocaloric and isonitrogenous as $T_1$ ) and 2% zeolite supplemented diet ($T_3$, supplemented to the control). Birds were housed in the batteries, and feeds and water were fed ad libitum. The results of Experiment 1 showed that birds fed control diet were heavier than those fed 2% zeolite supplemented diet up to 4th wks of age. However, birds fed the latter grew more at 5th and 6th wk. Overall weight gain, feed intake and feed efficiency were not significantly different. The results of Experiment 2 showed that there were no significant differences in weight gains, feed intakes and feed efficiencies among treatments during starter, finisher to overall period. The moisture levels in excreta of the birds were significantly (P＜0.01) less when zeolite was included in the diets and those in excreta significantly (P ＜ 0.01) increased as the ambient temperature increased. Economical efficiency expressed in fed cost (won) required per kg weight gin were 346.55 (T$_3$),348.34 ($T_1$ ) and 359.85 ($T_2$).

• Korean Journal of Poultry Science
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• v.37 no.4
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• pp.383-388
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• 2010

The chicken eye can discriminate light color, and different light wavelengths may affect reproduction ability. This study was carried out to identify effect of monochromatic light using light emitting diode (LED) in laying hens. Chickens were exposed to white light (WL), blue light (BL), yellow light (YL), green light (GL) and red light (RL) made by using LED as well as incandescent light (IL) (control). All light sources were equalized to a light intensity of 20 lux. The results indicated that the age of first egg laying and 50 % egg laying in laying hens treated under RL is significantly younger than under other lights (P<0.05). And the ovary weight of laying hens reared under RL is significantly heavier than under other at from 16 to 20 wks (P<0.05). The largest number of eggs production was produced in a group with treated with RL by 59 wks of age compared with any other group. The egg weight of YL was greater than other treatment groups from 26 to 45 wks (P<0.05). The egg shell from hens treated with RL was the strongest and thickest at 20 wk (P<0.05). These results suggest that the egg quality of laying hens reared in different spectrum of LED can be different and RL may enhance the laying performance.

• Korean Journal of Poultry Science
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• v.32 no.3
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• pp.171-177
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• 2005

Two experiments were carried out to investigate the effects of level and feeding period of dietary Monascus culture on the performance and cholesterol content of serum and meat in broilers and growing ducks.0.0(C), 0.5(T1), 1.0(T2) and $1.5\%(T3)$ of iwonascus culture which contained 0.5% monacolin-k was added to commercial broiler diets, respectively, and ffd during 2~6 weeks of age in Experiment 1. In Experilnent 2, commercial broiler diet added $1.0\%$ of Monascus culture was fed during $6\~6(C),\;4\~6(T1),\;2\~6(T2)\;and\;0\~6(T3)$ weeks of age, respectively. Three replicates of 16 day-old broilers and 10 day-old growing ducks each were randomly assigned to floor pen, respectively. In Experiment 1, as the level of dietary Monascus culture increased, body weight and feed intake of broilers significantly decreased (P<0.05), whereas those of growing ducks tended to increase without significant differences among treatments. Feed/gain ratio both in broilers and growing ducks showed a trend to increase. Cholesterol contents of serum both in broilers and growing ducks significantly decreased(P<0.05), and those of breast and thigh meat tended to decrease as the level of dietary Monascus culture increased. In Experiment 2, as the feeding period of dietary Monascus culture increased, body weight gain(P<0.05) and feed intake of broilers tended to decrease whereas those of growing ducks increased without significant differences anions treatments. Feed/gain ratio also increased without significant differences. Cholesterol contents of serum both in broilers and growing ducks significantly decreased(P<0.05), and those of breast and thigh meat tended to decrease as the feeding period of dietary Monascus culture increased without significant differences.