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Effect of Various β-1,3-glucan Supplements on the Performance, Blood Parameter, Small Intestinal Microflora and Immune Response in Laying Hens

β-Glucan 제제들이 산란계의 생산성, 혈액 성상과 소장내 미생물 균총 및 면역 체계에 미치는 영향

  • Park, K.W. (Department of Animal Science and Technology, College of Industrial Science, Chung-Ang University) ;
  • Rhee, A.R. (Department of Animal Science and Technology, College of Industrial Science, Chung-Ang University) ;
  • Lee, I.Y. (DMJ Biotech Corp.) ;
  • Kim, M.K. (DMJ Biotech Corp.) ;
  • Paik, I.K. (Department of Animal Science and Technology, College of Industrial Science, Chung-Ang University)
  • 박광월 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 이아름 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 이인영 (㈜ 더멋진바이오텍) ;
  • 김미경 (㈜ 더멋진바이오텍) ;
  • 백인기 (중앙대학교 산업과학대학 동물자원과학과)
  • Published : 2008.06.30

Abstract

This study was conducted to investigate the effect of feeding diets supplemented with ${\beta}-glucan$ products on the performance, small intestinal microflora and immune response in laying hens. The ${\beta}-glucan$ products used in the experiment were $BetaPolo^{(R)}$ ; soluble ${\beta}-glucan$ of microbial cell wall origin, $HiGlu^{(R)}$ ; microbial cell wall origin, $OGlu^{(R)}$ ; oat origin, $BGlu^{(R)}$ ; barley origin. A total of 720 Hy-Line Brown laying hens of 40wks old were divided into 5 dietary treatments : T1 ; Control( C), T2 ; $BetaPolo^{(R)}$, T3 ; $HiGlu^{(R)}$, T4 ; $OGlu^{(R)}$, T5 ; $BGlu^{(R)}$. Each treatment was replicated 4 times with 36 birds/replicate housed in 2 bird cages, and arranged according to completely randomized block design. Feeding trial lasted 40ds under 16 h lighting regimens. There were significant differences among treatments in hen-house egg production feed intake and feed conversion. HiGlu treatment was significantly higher than OGlu treatments in hen-house egg production. ${\beta}-glucan$ supplemented treatments were lower than the control in feed intake and feed conversion ratio. All ${\beta}-glucan$ supplemented treatments were significantly higher than the control in eggshell strength. Eggshell color and Haugh unit tended to be lower in the supplemented group than the control. IgY concentration was not significantly affected by treatments. At $5^{th}$ week of experiment, however, IgY concentration tended to increase in the supplemented groups. Among the leucocytes parameters, WBC, heterophil, lymphocytes, monocyte and eosinophil concentration were lower in the supplemented groups than those of the control. Among erythrocytes, HCT(hematocrit) and MCV(mean corpuscular volume) were significantly affected by treatment. MCV of supplemented groups were higher than that of the control. Immunoglobulin concentrations in the birds were not significantly different among treatments. However, IgA concentration tended to be low in the supplemented groups than the control. The cfu of small intestinal microflora were not significantly different among treatments, but that of Cl. perfringens tended to be lower than the control. The result of this experiment indicateted that feeding ${\beta}-glucan$ to laying hens improve feed conversion ratio and eggshell strength. Also intestinal microflora and immune responses are modified.

본 실험은 Beta-glucan 제제들이 산란계의 생산성, 혈액성상, 소장내 미생물 균총 및 면역 체계에 미치는 영향을 측정하기 위해 실시하였다. 사양 실험은 40주령의 산란계(Hy-Line Brown) 720수를 선별하여 A형 2단 케이지에 대조구 포함 총 5처리구로 구성하여 처리당 4반복 반복당 36수씩 randomized block design으로 배치하였다. 시험 기간 동안 물과 사료는 자유 섭취하게 하였으며 일반적인 점등 관리(자연일조+조명:16 hr)를 실시하였다. 시험구는 대조구, $BetaPolo^{(R)}$ (미생물 발효 ${\beta}-glucan$, 수용성)구, $HiGlu^{(R)}$(미생물 발효 ${\beta}-glucan$)구, $OGlu^{(R)}$(Oat ${\beta}-glucan$)구 그리고 $BGlu^{(R)}$(Barley ${\beta}-glucan$)구 등 5처리구로 두었다. 생산성에 있어서 일계 산란율은 처리간에 유의한 차이가 없었으나 hen-housed egg production에 있어서 OGlu구가 HiGlu 구에 비해 유의하게 낮았다. 사료 섭취량과 사료 요구율은 처리간에 유의한 차이가 있었는데 모든 ${\beta}-glucan$처리구들이 대조구에 비해 낮았다. 계란 품질에 있어서 난각 강도는 대조구에 비해 모든 ${\beta}-glucan$ 처리구들이 유의하게 높았고 그 중에서 BGlu구가 가장 높았다. 난각색과 Haugh unit는 ${\beta}-glucan$ 처리구들이 대조구보다 낮았는데 특히 OGlu구가 가장 낮았다. 혈액성상에 있어 leucocytes는 전반적으로 ${\beta}-glucan$ 처리구들이 대조구보다 낮았다. Erythrocytes 중 적혈구 용적율(HCT)과 평균적혈구용적(MCV)은 처리간에 유의한 차이가 있었는데 MCV는 ${\beta}-glucan$ 처리구들이 대조구에 비해 높은 수준을 보였다. 특히 ${\beta}-glucan$ 처리구중에서 OGlu구가 leucocytes와 erythrocytes 수준이 가장 높았다. 소장내 미생물 균총도 처리간 유의적 차이가 없었으나 Cl. perfringens 의 경우 모든 ${\beta}-glucan$ 처리구에서 대조구에 비하여 감소하는 경향을 보였다. 난황내 IgY 함량에 있어서는 전체적으로 유의적인 차이는 없으나 시험 마지막 5기에 ${\beta}-glucan$ 처리구 들에서 증가하는 경향을 보였다. 혈액내 IgG, IgA 함량에 있어서는 유의적 차이가 없었다. 이상의 결과를 종합해보면 산란계에 ${\beta}-glucan$ 제제들의 급여가 사료 요구율과 난각 강도를 유의하게 개선시켰는데 혈액성상, 장내 미생물 균총, 면역 기능 등에 순기능적 개선에 의한 결과라고 사료된다.

Keywords

References

  1. Anadon A, Martinez-Larranaga MR 1999 Residues of antimicrobial drugs and feed additives in animal products. Rgulatory aspects. Livest Prod Sci 59:183-198 https://doi.org/10.1016/S0301-6226(99)00026-3
  2. Bohn JA, Bemiller JN 1995 (1$\rightarrow$3)-$\beta$-D-glucan as biolgical response modifier: A review of structure-functional activity relationships. Carbohydrate Polymers 28:3-14 https://doi.org/10.1016/0144-8617(95)00076-3
  3. Cassell GH 1995 ASM Task force urges broad program on antimicrobial resistance. ASM News 61:116-120
  4. Eisen EJ, Bohren BB, McKean HE 1962 The haugh unit as a measure of egg albumen quality. Poultry Sci 41: 1461-1468 https://doi.org/10.3382/ps.0411461
  5. Hatta H, Kim M, Yamamoto T 1990 A novel isolation method for hen egg yolk antibody, 'IgY'. Agric Biol Chem 54:2531 https://doi.org/10.1271/bbb1961.54.2531
  6. Hatta H, Sim JS, Nakai S 1988 Separation of phospholipids from egg yolk and recovery of water-soluble proteins. J Food Sci 53:425 https://doi.org/10.1111/j.1365-2621.1988.tb07721.x
  7. Jung KA, Chang YK 1995 Effects of cereal on lipid concentration of liver and serum in rats. Korean J Nutr 28:5-14
  8. Kim MK, Ryu KE, Choi WA, Rhee YH, Lee IY 2003 Enhanced production of (1$\rightarrow$3)-$\beta$-D-glucan by a mutant strain of Agrobacterium species. Biochem Eng J 16:163-168 https://doi.org/10.1016/S1369-703X(03)00032-9
  9. Kunin CM 1993 Resistance to antimicrobial drugs: A worldwide calamity Ann Intern Med 118:557-561 https://doi.org/10.7326/0003-4819-118-7-199304010-00011
  10. Lowry VK, Farnell MB, Ferro PJ, Swaggerty CL, Bahl A, Kogut MH 2005 Purified $\beta$-glucan as an biotic feed additive upregulates the innate immune response in immature chickens against Salmonella enterica serovar. enteritidis. Int J Food Microbiol 98:309 https://doi.org/10.1016/j.ijfoodmicro.2004.06.008
  11. Mancini G, Carbonara AO, Heremans JF 1965 Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2:235-254 https://doi.org/10.1016/0019-2791(65)90004-2
  12. Marlett JA 1991 Dietary fiber content and effect of processing on two barley varieties. Cereal Foods World 36:576
  13. McKillop N, MacIsaac J, Rathgeber B 2006 Feeding White Leghorn hens yeast beta-glucans to influence egg quality. Page 101 In : PSA 2006 annual meeting(abstract 197), Edmonton, Canada
  14. Melvin JS 1984 Physiological properties & cellular & chemical constituents of blood, Page 359 In: Duke's Physiology of Domestic Animals (10th Ed.). Comstock Publishing Co., NY
  15. NRC 1998 Nutrient requirements of poultry. National Research Council National Academy of Science Washington DC
  16. SAS institute 1996 SAS/$STAT^{\circledR}$ users guide release 6.12 Edition SAS Institute Inc Cary Nc USA
  17. Shim JH, Choi WA, Sang BC, Yoon DY 2002 Immune stimulating efficacy of insoluble $\beta$-1,3-glucan from Agrobacterium sp. R259 KCTC 1019BP. Yakhak Hoeji 46:459-465
  18. Vanbelle M 1989 The European perspective on the use of animal feed additives : A world without antibiotics, anabolic agents or growth hormones? Page191 In : Proceeding of Alltech's 5th Annual Symposium AlltechTech. LyonsTP(ed) Biotechnology in the Feed Industry, Publ, Nicholasville, KY
  19. Yoo BW, Choi SI, Kim SH, Yang SJ, Koo HC, Seo SH, Park BK, Yoo HS, Park YH 2001 Immunostimulatory effects of anionic alkali mineral complex solution Barodon in porcinel ymphocytes. J Vet Sci 2:15-24

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