• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.581-589
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• 2021

Using visible-near infrared(Vis-NIR) spectra combined with machine learning methods, the feasibility of quick and non-destructive classification of Convolvulaceae species was studied. The main aim of this study is to classify six Convolvulaceae species in the field in different geographical regions of South Korea using a handheld spectrometer. Spectra were taken at 1.5 nm intervals from the adaxial side of the leaves in the Vis-NIR spectral region between 400 and 1,075 nm. The obtained spectra were preprocessed with three different preprocessing methods to find the best preprocessing approach with the highest classification accuracy. Preprocessed spectra of the six Convolvulaceae sp. were provided as input for the machine learning analysis. After cross-validation, the classification accuracy of various combinations of preprocessing and modeling ranged between 43.4% and 98.6%. The combination of Savitzky-Golay and Support vector machine methods showed the highest classification accuracy of 98.6% for the discrimination of Convolvulaceae sp. The growth stage of the plants, different measuring locations, and the scanning position of leaves on the plant were some of the crucial factors that affected the outcomes in this investigation. We conclude that Vis-NIR spectroscopy, coupled with suitable preprocessing and machine learning approaches, can be used in the field to effectively discriminate Convolvulaceae sp. for effective weed monitoring and management.

• Korean Journal of Environment and Ecology
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• v.28 no.5
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• pp.523-530
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• 2014

One juvenile raptor which was not able to be identified due to its head damage was discovered on a roadside in Janggok-ri, Jori-eup, Paju on 28th June, 2011. The species was identified by DNA barcoding. After polymerase chain reaction (PCR) of the mitochondrial cytochrome c oxidase subunit I gene (COI), we obtained 695 bp sequences. We analyzed the obtained COI sequence with similar sequences from the BOLD systems and BLAST of the NCBI Genbank, and discovered that its sequence showed 100 % similarity values with the one of the five gray-faced buzzards which were previously researched. In addition, it was confirmed to be a female through sex determination using DNA. Such results are important information as it confirms the breeding of the gray-faced buzzards for the first time in 43 years since its breeding was last recorded in 1968, in Paju. Wildlife rescue center needs to work with adjacent consigned registration and preservation institutions when carcass of wild animals is collected or DNA samples are obtained for more accurate both species and sex identification through a systematic management system in the future. Furthermore, the obtained DNA sample of the gray-faced buzzard and COI gene, DNA barcode, could be used as reference standards for similar researches in the future.

• Food Science of Animal Resources
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• v.24 no.4
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• pp.355-360
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• 2004

In Korean beef market, one of the major problems is mislabeling or fraudulent distribution of Holstein dairy meat or imported beef as domestic Hanwoo meat. Therefore, there has been a great need for a development of technology to identify beef breeds in meat and meat products. This study was carried out to develop the accurate and reliable method for the identification of beef breed using PCR-RFLP marker of MC1R, MGF and TYRPl genes affecting coat colors in cattle. A single base substitution (G\longrightarrowT transition) at the codon for amino acid position 104 of MC1R gene was identified between Hanwoo and Holstein and Angus breeds. The change at this position creates Msp I restriction site in Holstein and Angus, but not in Hanwoo. When the DNA amplified products (537 bp) was digested with Msp I, Hanwoo meat showed a single band of 537bp, while two fragments of 329bp and 208 bp were observed in Holstein meat and Angus breed, respectively. Thus, breed-specific RFLP marker in the MC1R gene can be used to distinguish between Hanwoo meat and Holstein and Angus meats. In the RFLP genotype of MGF gene, the frequency of r/r type was 75％ in Manwoo, whereas the frequency of R/R was 80％ in Hereford breed. Holstein and Angus breeds showed 100％ for R/r type. Therefore, Hanwoo meat showed significant difference in the MGF genotype frequencies compared with those of Holstein meat and imported beef cattle breeds. However, TYRP1 gene showed the same genotype in all breeds examined. Thus, this TYRP1 gene can not be used as a molecular marker for breed identification. As a consequence, we suggest that RFLP markers of the MC1R and MGF coat color genes could be used as DNA marker for identification of Hanwoo meat from Holstein and imported meats.

• Journal of Life Science
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• v.29 no.5
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• pp.524-529
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• 2019

Medicinal plants resources are becoming important assets since their usages have been expanded to the development of functional foods for human health, cosmetics and pharmaceutical industries. However, their phylogenetic origins and names are different from each country and quite often they are mixed each other resulting in the confusion for consumers. Particularly when they are very similar based on their morphological characteristics and distributed, it is extremely difficult to differentiate their origins even by specialists. Therefore, identification of each plant species is important for standardizing herbal medicine. Thistle is a medicinal and perennial plant. Obtaining information about the genetic diversity of plant populations is highly important for conservation and germplasm utilization. Although thistle is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish from other similar species from different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from chloroplast genomic sequences to identify distinct Korean-specific thistle species via high resolution melting (HRM) curve analyses. We performed molecular authentication of four different kinds of thistle species from different regions using DNA sequences in the trnL-F and matK chloroplast intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific thistle species from different country.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2006.05a
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• pp.361-362
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• 2006

HUE05-1는 Bjerkandera adusta와 87.7% 정도만이 유사도를 갖고 있는 다른 새로운 종의 색도제거능력이 있는 균주로 판별되었으며, RO 16, RB 19, RB 49, RY 145, AO 10, AV 43, AB 350, DB 106등의 8가지 염료에 대하여 우수한 색도제거효과를 보였다.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1992.10a
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• pp.223-227
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• 1992

산업의 발전으로 정밀가공기술 및 측정기술 및 측정기술의 개발에 대한 요구가 급증하고 있으며, 특히 가공전 공작물의 장착정도, 팔레트 교환 위치정도와 가공후 가공정도 및 공작기계 자체의 정도 판별은 생산품의 정밀도와 직접 관계되는 중요한 요인이기 때문에 정확히 규명할 필요가 있다. 본 연구에서는 공작물의 장착오차와 기계오차가 존재하는 경우 Denavit-Hartenberg 접근법에 의하여 레퍼런스에 대한 공간상 linkage의 기하학적 표현을 Matrix Multiplication을 사용하여 Cutting Tool 과 Workpiece에 대한 공간상의 관계를 규명하였으며, 가공에 미치는 체적오차를 규명하고자 한다.

• Communications of the Korean Institute of Information Scientists and Engineers
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• v.5 no.2
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• pp.22-27
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• 1987

The generating method of syntax analyzer using production language (PL) developed by Evans [1] is efficient method, but it's very difficult to construct PL. So, this paper deals with algorithm generating PL from given BNF ALGOL grammar.

• The Korean Journal of Mycology
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• v.44 no.2
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• pp.103-107
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• 2016

To establish a rapid and accurate detection of Phytophthora pinifolia, which is a quarantine pathogenic fungus in Korea, a species-specific primer was developed based on the ras-related protein (Ypt1) gene. Species-specific primer based on the DNA sequences of Ypt1 gene amplified 193 bp polymerase chain reaction (PCR) product for P. pinifolia. The primer pair yielded the predicted PCR product size exactly in testing with target pathogen DNAs, but not from the other 10 species of Phytophthora and 14 species of other phytopathogenic fungi. The primer pair also showed only the species-specific amplification curve on realtime PCR on target pathogen DNA. The detection sensitivity of real time PCR using species-specific primer pair was 10 to 100 times higher than conventional PCR, with 1 to $10pg/{\mu}L$.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.6
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• pp.1042-1049
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• 2000

Irradiation-induced volatile flavor compounds in irradiated (1, 3, 5, 10 kGy) beef were analyzed by liquid liquid continuous extraction (LLCE) and gas chromatography/mass spectrometry (GC/MS) methods. One hundred fifty volatile compounds were detected in irradiated beef. These compounds were composed mainly of 71 hydrocarbons, 35 aromatic compounds, 15 aldehydes, 7 ketones, 4 acids, 6 esters and 12 miscellaneous compounds. Among these, only 6 volatile compounds including (E) -2-hexenal, nonene, 2-nonenal, cyclodecene, dodecene and cyclododecene were detected as irradiation-induced volatile flavor compounds, comparing with unirradiated beef meat. However, 4 volatile compounds, such as cyclodecene (r=0.88), (E)-2-hexenal (r=0.85), nonene (r=0.74) and 2-nonenal (r=0.70), having a positive correlation coefficient with the increment of irradiation dose, were considered as marker compounds for detecting irradiation dosages in irradiated beef.

• Asia Marketing Journal
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• v.1 no.2
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• pp.56-76
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• 1999

금융시장에서의 경쟁이 치열해지면서 이제 국내 소비자금융기관들에게 수익성위주의 내실경영은 피할 수 없는 지상과제로 부상하고 있다. 이러한 목표를 성취하기 위해서는 우량고객을 위주로 한 기반강화와 철저한 사후관리를 통한 수익성향상이 이루어져야 한다. 특히, 자금운용처로 부상하고있는 개인고객들을 대상으로 하는 효과적인 대출마케팅의 수행은 소매금융기관들의 수익성제고에 절대적이라고 할 수 있다. 즉, 수익성을 지향하기 위해서는 고객관리를 보다 더 철저하게 하여야 하며 이를 위해서는 신용 및 수익성에 근거해서 산출된 평점에 따라 개인별 관리를 차별화하는데 있다고 할 수 있다. 본 연구에서는 우량고객들을 대상으로 대출마케팅을 활성화시키기 위한 고객평점모형의 효과성에 대해서 고찰해 보고자 하였다. 이를 위해서 신용평점모형에 대해서 자세히 알아보고 이어서 수익성에 근거한 평점모형에 대해서도 이론적으로 살펴보았다. 그리고 두 모형의 효과성을 비교하기 위해서 판별분석을 사용하여 우량 및 불량고객에 대한 예측력을 분석해 보았다. 분석결과 제1종오차에 대해서는 신용평점모형이, 제2종 오차에 대해서는 수익성평점모형이 보다 정교한 예측력을 나타냈다. 결론적으로 두 모형의 사용이 병행되는 통합적인 고객평점모형의 적용이 제안되어 졌다.