• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.200-201
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• 2005

• 한국약용작물학회:학술대회논문집
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• 2012.05a
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• pp.61-62
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• 2012

• Journal of Korean Society of Forest Science
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• v.108 no.3
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• pp.290-295
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• 2019

This study was carried out to investigate the possibility of establishing a reproductive system for the seed of Pedicularis hallaisanensis, which is in the endangered wild species II class in Korea. The seed of P. hallaisanensis is egg-shaped, and the seed coat is dark brown. The embryo was identified as a dwarf type by the seed section. The seed length was $0.47{\pm}0.07mm$, width $0.16{\pm}0.006mm$, and thickness $0.12{\pm}0.01mm$. The weight of one seed was $0.0003{\pm}0.0001mg$, and 1000 seeds weighed $4.59{\pm}0.02mg$. The degree of seed viability was 75.33% by the tetrazolium (TZ) assay. The highest germination rate of P. hallaisanensis seed was 71% after 4 weeks of storage at $4^{\circ}C$. However, the germination rate tended to decrease gradually over a longer storage period. The germination rates after 6 or 8 weeks of storage at $4^{\circ}C$ were 64% and 60%, respectively. We used two host plants, Artemisia princeps and Dendranthema zawadskii, to determine the effect of host plants on P. hallaisanensis seed germination. The germination of P. hallaisanensis mixed with A. princeps or D. zawadskii started at 53.5 and 62.5 days after sowing, respectively. We did not find any germination 164 days postsowing with both host plants. When A. princeps and D. zawadskii were used as host plants for P. hallaisanensis seed germination, P. hallaisanensis seed germination rates were 45.5% and 19.5%, respectively. The average time to germination was 70.2 days for A. princeps, and 46.8 days for D. zawadskii.

• Journal of Bio-Environment Control
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• v.22 no.3
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• pp.262-269
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• 2013

This study was carried out in order to examine the germination characteristics of Ligularia fischeri seeds, and it was to develop the more efficient pre-treatment and production system of the seeds. It was performed by two ways - temperature control (10, 15, 20 and $25^{\circ}C$) and shading treatment (Full sunlight, 35%, 50%, 75% and 95% of full sunlight). Seed pre-treatment before the each experiment was carried out by temperature (with low temperature and wetting treatment (LTW) for 0, 15, 30, 45 and 60days) and shading treatment (with drying at room temperature (DRT), drying at low temperature (DLT) and water soaking (WS) for 2 days). Seeds of L. fischeri were, regardless of seed pre-treatment, germinated well at $10^{\circ}C$ and the more temperature went up, the more germination rate went down. As a result of surveying shading treatment, 75% shading with DRT was the highest germination rate (68.1%) and 95% shading with WS was the lowest germination rate (48.6%). It was showed over-growth under 95% shading treatment experiment and withered in the full sunlight. As a result of surveying the whole experiment, L. fischeri seeds pre-treated with LTW for 15 days germinated well at $10^{\circ}C$ and under 50~75% shading treatment.

• Korean Journal of Weed Science
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• v.7 no.1
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• pp.19-28
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• 1987

These experiments were conducted to recognize the germination change in relation to storage period, using 25 kinds of field-collected weed seeds stored under the dry and low temperature ($4{\pm}1^{\circ}C$), and to establish a simple germination induction methods in some kinds of them. No or weak dormancy was observed in Lepidium virginicum, Rumex japonicus, Cirsium japonicum var. ussuriense, Aeschnomene indica and Ludwigia prostrate. The germination of Digltaria sanguinalis, Portulaca oleracea, Amaranthus spp., Echinochloa crux-galli, Polygonum persicaria, Cyperus iria, and Aeschynnmene indica was enhanced by $H_2SO_4$ treatment for 20, 2, 2, 50, 40, 3 or 5 minutes, respectively. Favorable germination in Polygonum persicaria and Echinochloa crus-galli was obtained by soaking in water for 25 days and 50 days, respectively. Moisture treatment for 21 days and 14 days caused the enhancement of germination in Cyperus difformis and Aneilema keisak, respectively. Combination of various treatments in Cyperus iria was most effective when the seeds treated with $H_2SO_4$ for 3 minutes incubated in alternating temp. after soaking in 0.2% $KNO_3$, but their effect on Digitaria sanguinnlis was little.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.4
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• pp.382-388
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• 1994

For the comparision of the differences in dry matter weight and the chemical composition of soybean seeds in relation to utilization styles and storage durations, the seed coats and cotyledons including embryo axis separated from the seeds of 3 recommended cultivars stored for 3 years and of 5 recommended cultivars and 2 local varieties with black seed coat and green cotyledons were observed in dry weight, germination percentage, protein, oil, soluble sugar, oilgosaccharide, and starch. In the seeds stored for 3 years, dry weight and germination rate were decreased distinctively. This decrease of seed dry matter resulted from the decrease of the cotyledon weight, and the ratio of seed coat to whole seed was increased. For the change of chemical compositions by the decrement of seed dry weight during the storage, protein, oil, and carbohydrate contents of the seed coat were decreased, and protein content of the cotyledon was markedly decreased in the seeds stored for 3 years. The ratio of seed coat to whole seed was higher in small seed than large seed cultivars. In the local varieties, protein and oil contents were 43.59% and 18.25% in the cotyledons, respectively, which were lower than those of recommended cultivars. But starch and sugar contents were 5.70% and 11.58%, respectively, which were higher than those of recommended cultivars. Sucrose, raffinose and stachyose contents were also higher in the cotyledon of the local varieties.

• Journal of Plant Biology
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• v.35 no.1
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• pp.53-60
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• 1992

This study has been carried out to investigate the ultrastructural changes in the associated with the disintegration of the storage materials in endosperm cell of ginseng (Panax ginseng C.A. Meyer) seed during after-ripening with light and electron microscope. The protein body of endosperm cells near the umbiliform layer showed various degenerative patterns, and so electron density of proteinaceous matrix was gradualJy decreased during afterripening. These results indicate that the decomposition of endosperm was already initiated during after-ripening. As the degeneration of endosperm was more progressed after the dehiscence of seed, non-decomposed part of protein body appeared amorphously with high electron density. Decomposed protein bodies were vacuolized with the loss of their matrix and gradually expanded by fusion. Also, spherosomes were gradually dissolved with the lowered electron density during the degeneration of endosperm. The vesicles of dictyosomes near the cell wall are observed in endosperm contacting with umbiliform layer and are fused with plasma membrane. Umbiliform layer which was the complex of the decomposed remnants of lysis and materials has strong stainability for toluidine blue and basic fuchsin.

• Food Science and Preservation
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• v.16 no.6
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• pp.971-976
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• 2009

The consumption of raw sprouts has increased in popularity worldwide because the food is natural and healthy. However, in Korea, nothing is known on the safety standards of sprout producers or changes in the microbial populations of sprouts during sprouting. We evaluated the microbial safety and quality of sprouts during each step in the sprouting process. Bacteriological analysis showed that seeds had a Total Plate Count (TPC) ranging from 3.04 - 5.21 log CFU/g and coliform counts ranging from 1.80 - 3.86 log CFU/g. TPC and coliform counts increased rapidly during the sprouting process to attain values of 6.99 - 8.26 and 3.70 - 7.15 log CFU/g, respectively, regardless of decontamination of seeds with commercial sanitizer. TPC and coliform counts were on high level after sprouts were washed. Escherichia coli was detected in samples of domestic radish sprouts at all stages from seed to storage, rape sprouts in the stages from soaked seed to storage, and red radish sprouts during sprouting, and no sanitizer was used in any of these processes. Untreated red radish sprouts were also positive for Bacillus cereus at all processing steps and Listeria monocytogenes after germination. However, pathogens were not detected at any sprouting stage of seeds treated with sanitizer. It is necessary to carefully control commercial sprouting, and to develop HACCP guidelines applicable to all sprouting processes, commencing at the first step in raw seed production.

• Food Science and Preservation
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• v.16 no.2
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• pp.186-191
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• 2009

An electron beam or gamma-rays(0-5 kGy) were applied to two popular commercial sprout seeds, those of radish and red cabbage, and both physiological and microbiological qualities during germination were examined. Total bacterial counts of radish and red cabbage seeds were 5.02 and 2.86 log CFU/g, respectively, and were reduced by 2-3 logs after irradiation, but nonirradiated and irradiated samples showed similar microbial populations on day 5 after germination, although day 3 levels of irradiated samples were $23{\pm}2%$ lower. Coliforms were absent in both seed types. However, they were detected from the first day of germination, and rose to 4-5 log CFU/g on the fifth day. Coliforms were reduced by about 1-2 log CFU/g in 1 kGy-irradiated samples and were absent in the samples irradiated at 3 kGy. Irradiation at 1 and 3 kGy decreased germination by about 7% and 18%, respectively. Sprout yield and length were also significantly affected by irradiation, with no apparent difference between samples treated with the electron beam and gamma-rays.

• Food Science and Preservation
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• v.14 no.5
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• pp.487-491
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• 2007

This study was conducted to investigate the effect of treatment with squeous chlorine dioxide and hot water on the germination of radish (Raphanus sativus L.) seeds, and reduction of microbial load on the seeds. Increases in treatment and the concentration of aqueous chlorine dioxide in water resulted in increasing reductions in the counts of total aerobic microbes. Seeds treated with aqueous chlorine dioxide (100 ppm/20min, 200ppm/20min) showed about a 10-fold decrease in microbial loads. Germination of seeds was not adversely affected by any treatment tested, although the germination rate of seeds in the group treated at $55^{\circ}C$ for 20 min was reduced by 10% compared to that of control. Combined treatment with hot water and aqueous chlorine dioxide yielded better out comes in both microbial reduction and seed germination rate than did single treatments. A combined treatment with 100 ppm aqueous chlorine dioxide and hot water($45^{\circ}C$ or $50^{\circ}C$) resulted in about a 100-fold decrease in microbial load whereas germination rate showed only a slight increase to $97.0{\sim}97.7%$. Total aerobic microbial counts in radish seeds were decreased by aqueous chlorine dioxide and hot water treatment in the order. aqueous $CIO_2$+ hot water > aqueous $CIO_2$ > chlorinated water > hot water > control.