• Journal of agriculture & life science
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• v.43 no.5
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• pp.39-42
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• 2009

Soybean is an important sources of plant proteins for human and animal nutrition. The use of soybean proteins has been expanded in the food industry due to their excellent nutritional benefits. But, Soybeans contain allergenic proteins that cause allergies to sensitive individuals. ${\beta}$-conglycinin(7S globulin) and glycinin(11S globulin) are the major components of storage protein in soybean. ${\beta}$-conglycinin consists of three subunits, ${\alpha}^{\prime}$, ${\alpha}$, ${\beta}$ and exhibits poorer nutritional and food processing properties than glycinin. There is a great deal of interest in the development of soybean lines with reduced amounts of ${\beta}$-conglycinin. The objective of this study was to determine the inheritance of ${\alpha}^{\prime}$-subunit protein in 7S globulin. F2 population was developed from the cross of "Jinpumkong2ho"(${\alpha}^{\prime}$-subunit presence) and PI506876(${\alpha}^{\prime}$-subunit absence) parent. Total 98 of F2 seeds were obtained and analyzed for the segregation of ${\alpha}^{\prime}$-subunit protein by SDS-PAGE. Among 98 F2 seeds, 70 F2 seeds showed ${\alpha}^{\prime}$-subunit protein and 28 F2 seeds did not show ${\alpha}^{\prime}$-subunit protein. The segregation ratios of 3 : 1 for presence and absence of ${\alpha}^{\prime}$-subunit protein were observed(${\chi}^2=0.667$, P=0.414). These data indicate that presence and absence of ${\alpha}^{\prime}$-subunit protein is controlled by a single major gene and might be useful for strain selection of 7S protein reduced soybean.

• Food Science and Preservation
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• v.25 no.1
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• pp.90-97
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• 2018

Peach seeds contain a large amount of phenolic components and exhibit excellent physiological effects in various diseases. We examined the antioxidant effects of stone and seed of three peach cultivars (Miwhang, MH; Kanoiwa hakuto, KH; and Cheonhong, CH) by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, ferric reducing activity of plasma (FRAP) assay, and cupric ion reducing antioxidant capacity (CUPRAC) reduction. The results showed that the stone extracts of CH had higher levels of total phenols and flavonoids than those of the other cultivars do, and the stone extracts of KH and CH have the potential to reduce DPPH, FRAP, and CUPRAC activities. In addition, we found that KH, MH, and CH stone extracts decreased nitric oxide generation in RAW 264.7 and BV2 cells. The total phenol and flavonoid contents had no significant correlation with anti-oxidant activities. On the other hand, the anti-inflammatory activity had a low linear correlation with anti-oxidant activities and total phenol and flavonoid contents. The present results suggest that the correlation between antioxidant and anti-inflammatory effects of stone and seed, and the appropriate combination of the stone and seed extracts could be used as an anti-inflammatory treatment and prevention material, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.1
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• pp.44-51
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• 1995

This investigation was conducted to determine the effect of the optimum planting date and place for good seed production on growth variation, grain yields, different of variety, infection of seed by fungi, and seed germination and vigor after room storage. Early maturity Korean soybean variety, Keunolkong, and introduced vegetable soybean variety, Fukura-edamame, were planted at field of YAES. and high cool land of Sajapyong in Milyang（altitude, 850m） on May 15 （early）, June 15 （mid.）, and July 15 （late） in 1991. The emergence and vegetative period gradually increased in the early planting date. The grain yield, seed weight, pod number, healthy grain yield also increased when Keunolkong was planted on early date. The healthy seed rate, Keunolkong increased in the early planting date, whereas that of Fukura also increased in the late planting date. Infection ratio of grain to phomopsis seed decay （Phomopsis spp. ' Diaporthe phaseolorum） in Fukura steadily increased in the early planting date. Infection ratio of grain to purple seed stain （Cercospora kikuchii） generally increased in the mid and late planting date. The seed germination and seedling vigor after room storage from five to six months gradually increased in the late planting date. And seed germination of Fukura rapidly decreased in the early planting date. Therefore, the optimum planting date for good seed production in early maturity vegetable soybean was June 15 in terms of harvesting time avoid a high temprature and humidity.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.6-6
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• 2018

백두대간은 백두산에서 시작된 큰 산줄기로 금강산, 설악산, 덕유산 등을 거쳐 지리산까지 이어지는 우리나라 중심 산줄기이다. 백두대간은 우리 민족 고유의 사회 문화적 가치는 물론 자연 생태적 측면에서 매우 중요한 지역이다. 백두산에서 지리산까지 약 1,400km, 남한 지역은 약 700km가 남북으로 길게 연결되어 우리나라 주요 산들을 연결하는 한반도 중심축을 형성하고 있다. 백두대간은 생물다양성의 보고이자 주요강의 발원지로 중요한 역할을 하고 있다. 이러한 주요 지역에 위치함 국립백두대간수목원은 수목원 정원의 조성 및 진흥에 관한 법률에 근거하여 기후대 및 식생권역별 국가수목원의 확충 계획에 따라 온대 북부 권역에 조성되었다. 백두대간 산림생물 다양성 유지와 산림생물자원의 보전 및 활용을 위한 다양한 연구를 수행하고 있다. 국립백두대간수목원의 시드볼트는 생물다양성 보존과 산림생물유전자원의 지속가능한 활용을 위하여 종자보존 협력 네트워크를 구축 하고 있다. 또한 아시아를 포함한 전세계 야생 종자를 수집하기 위한 기후대 생태권역별 종합계획 수립과 표준화 모델 개발을 통해 체계적인 협력체를 구축 중이다. 종자의 안정적인 장기 보존을 위한 형태학적, 분자생물학적 연구를 통해 종자의 저장특성 연구 및 수명연구를 통해 예측 모델을 연구하며 이를 기반으로 생태계 복원을 위한 종자 활용 연구를 수행하고 있다. 국립백두대간수목원은 백두대간 생물종의 조사, 생태계 보전 복원연구를 위한 현지 조사, 서식지외 보전 기관 역할, 유용자원 발굴 및 연구 등 백두대간 생물다양성 보전을 위한 다양한 역할을 하고 있다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.24-24
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• 2023

본 실험은 한반도 극지 고산식물인 진퍼리꽃나무[Chamaedaphne calyculata (L.) Moench] 종자의 형태적 특성을 조사하고 변온조건과 저온처리 및 GA3 처리가 발아에 미치는 영향을 구명하고자 수행하였다. 진퍼리꽃나무 종자는 국립수목원 전시원에서 채종 후 4℃에 저온건조 저장하였으며 전자현미경(DVM6, Leica Microsystems GmbH, Wetzlar, Germany)을 이용하여 종자와 배의 길이를 측정하였다. 2022년 3월에 growth chamber 내에서 32일간 15/6℃, 25/15℃ 변온조건하에 배양하였으며, 저온층적처리(0, 40일) 및 GA3 처리(0/500/1,000mg·L^-1) 처리에 따른 발아 특성을 구명하였다. 진퍼리꽃나무 종자의 최종발아율(FGP)은 25/15℃ GA3 1000mg·L^-1 처리에서 42.2%로 가장 높게 조사되었다. 평균발아일수(MGT)는 15/6℃ 저온층적 처리에서 22.3일로 가장 높았으며, 대부분 15/6℃ 변온조건에서 25/15℃ 변온조건보다 6∼8일 높았다. 최종발아일의 평균은 약 12일로 확인되었다. 저온 층적처리 결과, 최종발아율(FGP)은 온도조건 25/15℃, 15/6℃에서 각각 32.2%와 11.1%를 보여 무처리구에서 조사된 24.4%와 10.0%보다 각각 7.8%, 1.1% 높게 나타났다. 호르몬처리 결과, 동일한 발아조건에서 GA3 500mg·L^-1 처리보다 1000mg·L^-1 처리에서 더 높은 발아율을 보였다. GA3 1000mg·L^-1 처리에서의 최종발아율(FGP)DKSH은 25/15℃, 15/6℃ 각각 42.2%와 30.0%를 보여 무처리구 보다 각각 17.8%, 20% 높게 나타났다.

• Journal of Bio-Environment Control
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• v.15 no.2
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• pp.177-183
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• 2006

The summer production of sweet pepper in Korea which cultured in the highland area such as Hapchon is essential for a year-round export, but has the problem, fruits lose easily those marketability. This study was carried out to find changes of fruit quality in response to storage on sweet pepper cultivars in summer. Seven cultivars of sweet peppers, 'Jubilee', 'Plenty', 'Goal', 'Romeca', 'Derby', 'Midas' and 'Maximalia' were obtained iron growers in Hapchon which was highland of 600-800m from sea level and stored at $10^{\circ}C$ with box packing. Fruit quality was evaluated every 4days up to 4weeks. The weight loss of 'Jubilee' was the lowest, that of 'Romeca' was the highest. The firmness of 'Goal' was maintained highly for 4weeks and the red cultivars was higher than those of other color cultivars. The lightness of 'Jubilee' was sensitively affected by storage duration. Seeds of 'Plenty' and 'Derby' were plentifully browned after 4weeks storage. The occurrence of fruit rotting was after 16-204ay storage and those of 'Plenty' and 'Maximalia' were the earliest. The marketability of 'Jubilee' and 'Romeca' maintained by 70% for 16 days was the highest, but most cultivars lost those by 20% after 24days storage. It may be suggested that the marketability of sweet pepper producted in summer can be stored at $10^{\circ}C$ for 2weeks to maintain that of 80%.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.158-162
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• 2000

Histochemical distribution and varietal variation of schizandrin and oil compounds, and the changes of their extraction yield were investigated in fruits of collected Schizandra chinensis including Inje cultivar. In histochemical analysis on the distribution of schizandrin and oil in fruits of Inje cultivar, higher concentrations of them were found in the seed (1.01% and 27.6%, respectively) than in the epicarp and mesocarp of the whole fruit. Average contents of schizandrin in fruits and oil in seeds of collected lines were 0.84% and 27.9%, respectively. The mean composition of fatty acids in seeds oil was 3.6% of palmitic acid, 0.6% of stearic acid, 19.7% of oleic acid, 73.0% of linoleic acid, and 3.1% of linolenic acid, showing high composition(95.8%) of total unsaturated fatty acid. Oil extracted from seeds of Inje cultivar contained 4.29% of schizandrin, indicating that seed oil contained much schizandrin, a bioactive lipid-soluble compound. Compared with 80% methanol extraction in fruits and seeds, yields of schizandrin and oil were lower showing 23.8% and 17.3%, respectively in boiling water extraction of the fruits and seeds without grinding. The seeds soaked with water during four months contained 1.18% of schizandrin and 25.2% of oil, whose contents were similar to those of the seeds stored at room temperature. These results demonstrated that the seed in the whole fruit could be utilized as a source to extract its functional oil and bioactive lipid-soluble compounds like schizandrin, especially after using Schizandra fruits for the beverage manufacture.

• Korean Journal of Plant Resources
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• v.30 no.4
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• pp.372-377
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• 2017

This research was performed to develop mass propagation method of Alpine modest primrose (Primula modesta var. hannasanensis T.Yamaz.) for improving the conservation and utilization of the species. Seeds were collected on August 2011 in Jeju-city and well-selected seeds were dry-stored at $4{\pm}1.0^{\circ}C$. Seed size ranged $0.44{\pm}0.07{\times}0.61{\pm}0.04mm$, and weight of 1,000 seeds was $51.78{\pm}0.021mg$. Thus the seed was classified as 'dwarf seeds'. As the result of dormancy characteristics, moisture content of freshly matured seeds increased rapidly by water-soaking treatment and seeds did not germinate at $20^{\circ}C$ for 4 weeks under the light condition. Therefore, seeds were estimated to have physiological dormancy. Germination conditions of dry-stored seeds were found to be $20^{\circ}C$ and light condition, and seeds did not germinated under dark condition regardless of all temperature regimes. Therefore, Alpine modest primrose seed was considered as photoblastic type. Percent germination (PG) and germination energy (GE) were greatly improved by soaking the seeds in $GA_3$ and kinetin for 24 hours. Especially, 200~500 mg/L $GA_3$ treatment resulted in the highest PG (95.5%) and GE (98.3%). So, chemical treatment such as $GA_3$ was thought to be a useful method for raising seedling uniformly.

• Korean Journal of Plant Resources
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• v.36 no.4
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• pp.390-401
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• 2023

The purpose of this study was to identify the propagation characteristics of Diabelia spathulata (Siebold & Zucc.) Landrein through seeding and cutting methods. The seed germination rate was meager (0.22~3.56%). In hardwood cutting, the highest rooting rate was 36.1% in sand + perlite (2:1) and 37.8% in 1000 PPM of IBA pre-treatment. Softwood cutting showed the highest rooting rate when the cutting diameter was 3 to 5 mm and the cutting length was 7 to 8 cm. In particular, the highest rooting rate of 80% was observed in the sand among the 8 cultivation media treatments. In the case of root cuttings, the rooting rate was 83.3~96.7% in all cultivation media. The highest rooting rate was observed when the diameter of the cutting was 7 to 8 mm, and the length of the cutting was about 10 to 11 cm. Among cutting methods, root cutting showed the highest rooting rate. Division showed a 100% rooting rate regardless of size or growth status. For Diabelia spathulata (Siebold & Zucc.) Landrein, propagation by cuttings was more efficient than seeding. However, Further research on the morphophysiological dormancy and dormancy break of seeds is needed for seed propagation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.3
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• pp.278-284
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• 1994

The sulfur amino acid composition in soybean (Glycine max L.) seeds may be an essential characteristic of new cultivars for some animal diets. Variation in seed storage protein among genotypes might make it possible to improve the quality of seed protein by genetically altering seed storage protein composition through plant breeding. This study was carried out to determine if mutant strains have potential for improving seed protein quality in soybean. Ten mutant strains had a distinct characteristic of seed storage protein subunits. Among the mutant strains, the sulfur amino acid compositions(methionine plus cystein) of Keburi(P.I.417016), Keburi(P.I.506817), and P.I.54608-1 were relatively higher than those of the others and were 1.9, 2.1, and 1.8%, repectively, which might be due to low levels of ${\alpha}$, ${\alpha}$', and ${\beta}$ subunits of 7S protein. Therefore, it is concluded that the mutant strains, Keburi(P.I.417016), Keburi(P.I.506817), and P.I.54608-1 appear to be potential materials for a breeding program for improving sulfur amino acid composition, and the others also seem to be possible breeding materials for other uses.