• Korean Journal of Plant Taxonomy
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• v.49 no.2
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• pp.145-178
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• 2019

The genus Pseudostellaria (Caryophyllaceae) in Korea was investigated in order to clarify the taxonomic positions and scientific names among the species in this genus based on morphological characteristics. P. ${\times}$ bohyeonsanensis and P. ${\times}$ seoraksanensis share several characteristics (trichome on the sepal margin and midvein, five petals, and stem reclining to the ground after flowering) with P. davidii. P. ${\times}$ bohyeonsanensis, resulting from natural hybridization of P. davidii and P. okamotoi var. longipedicellata, has sterile fruit and a long pedicel (2.2-6.0 cm). P. ${\times}$ seoraksanensis, a natural hybrid of P. davidii and P. palibiniana var. palibiniana, has sterile fruit and a short pedicel (1.1-1.8 cm). P. okamotoi var. longipedicellata shares several characteristics (long pedicels of chasmogamic flowers that elongate to the ground when bearing fruit and cleistogamic flowers with a long pedicel) with the related taxa P. okamotoi var. okamotoi. P. okamotoi var. longipedicellata has slender root tubers, glabrous pedicels, and 5-9 petals. P. palibiniana var. gageodoensis is described from Gageodo Island. It is similar to P. palibiniana var. palibiniana having chasmogamic flowers at the stem terminal, 5-7 petals, and a short pedicel, but P. palibiniana var. gageodoensis is distinguished by 1-2 rows of trichomes on the pedicel, a large sepal size, a large petal size, and a branched stem at its base. P. ${\times}$ biseulsanensis, from a natural hybrid of P. heterophylla and P. okamotoi var. longipedicellata, has a solitary aerial stem, a chasmogamic flower at the stem terminal, a shallow marginate or bitten petal apex, one row of trichomes on the pedicel, and sterile fruit. P. ${\times}$ segeolsanensis, from a natural hybrid of P. palibiniana var. palibiniana and P. okamotoi var. okamotoi, is described from Segeolsan Mt. It has numerous aerial stems, a chasmogamic flower at the stem terminal, 1-2 rows of trichomes on the pedicel, and fertile fruit. Consequently, the genus Pseudostellaria in Korea has been classified into eight species, four hybrids, two varieties, and five forms.

• Journal of the Korean Society of International Agriculture
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• v.30 no.4
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• pp.347-356
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• 2018

Genetically modified (GM) crops have been increased continuously over the world and concerns about the potential risks of GM crops have also been increasing. Even though GM crops have not been cultivated commercially in Korea, it should be necessary to develop the safety assesment technology for GM crops. In this study, we investigated the influence of heading date difference on gene flow from GM to non-GM rice. In the experimental plot design, The PAC GM rice was placed in the center as a pollen donor and non-GM rice were placed in eight directions as pollen receivers. Five pollen receiver rice cultivars were Unkawng, Daebo, Saegyejinmi, Nakdong-byeo, and Ilmi which had different flowering times. A total of 266,436, 300,237, 305,223, 273,373, and 290,759 seeds were collected from Unkawng, Daebo, Saegyejinmi, Nakdong, and Ilmi, respectively, which were planted around PAC GM rice. The GM${\times}$non-GM hybrids were detected by repeated spraying of herbicide and PAT immunostrip assay. Finally, the hybrids were confirmed by PCR analysis using PAC gene specific primer. The hybrids were found in Nakdong-byeo which had the same heading date with PAC GM rice. The hybridization rate was 0.0007% at Nakdong-byeo plot. All of GM${\times}$non-GM hybrids were located within 2 m distance from the PAC GM rice zone. The physiological elements including rice heading date were found to be important factors to determine GM?rice out crossing rate with GM rice. Consideration should be taken into for many factors like the physiological elements of field heading date of rice cultivars to set up the safety management guideline for prevention of GM rice gene flow.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.346-361
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• 2021

The range of D. spathulata identified in this survey was between N 35° 24' 58" ~ N 35° 26' 35", E 129° 05' 43" ~ E 129° 07' 04". It is located at an altitude of 98~592 m. The soil pH was strongly acidic in the range of 4.2~4.9, with a canopy openness of 18.56% and a chlorophyll index of 36.74 ± 2.80. As a result of the TWINSPAN analysis, 20 plots of 100 m² each were divided in 4 communities: Pinus densiflora community, Quercus monglica-Diabelia spathulata community, Quercus serrata-Diabelia spathulata community and Carpinus tschonoskii subassociation. The result of species diversity was 0.7615, and evenness and dominance were found to be 0.6077 and 0.3923, respectively. The height of D. spathulata is up to 3.4 m, and the average height is 1.1 m, with most of the species distributed as shrubbery and herbaceous. The average population density of the 20 plots was 1.635 individuals/m², the height range of flowering was 1.0 ~ 1.8 (aver. 1.39 m) and the rate of flowering was 27.37%. It's propagation pattern was mainly formed by extending the rhizome to the side, creating a colony of ground stems.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.1
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• pp.18-28
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• 2021

The growth period and productivity of cropping system of winter wheat-rice, winter wheat-bean and winter wheat-grain corn for 4 years from 2015 to 2018 were compared at the experimental field of National Institute of Crop Science in Miryang city. The harvest period of winter wheat was in mid-June, and summer crops were sown (transplanted) in late June. In transplanting of rice in late June, there was no difficulty in securing the heading of panicle and the yield of rice, but there was a lot of trouble in sowing wheat in proper time because the harvest time of rice was delayed to early November due to late maturity of rice, particularly in the mid-late maturing cultivar. There was no problem in soybean planting after winter wheat because the proper period of soybean planting is late-June. In addition, there was no problem in winter wheat sowng after soybean because the maturity period of soybean was mid-October. Selection of grain maize in double cropping with winter wheat in terms of growing periods, was desirable because grain maize had the fastest maturity among summer crops. In double cropping of winter wheat-summer crops, wheats combined with soybean and grain maize showed stable yields during three years, but there was a risk of yield declines in the wheat combined with rice in heavy rainfall year. It was possible to secure high yields in three summer crops as yields of rice, soybean, and corn were 600, 350, and 800 kg/10a, respectively. Summer crops with medium maturity was recommended because of no significant difference in yield between medium maturity and medium-late maturity cultivar. Soil physical properties were improved in soils cultivated with soybean and grain maize. Therefore, It was thought that double cropping systems of winter wheat with soybean and grain maize were superior to that of winter wheat with rice in terms of connecting period between winter wheat - summer crops and improvement of soil physical properties, and total income, particularly in soybean.

• Korean Journal of Plant Resources
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• v.35 no.5
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• pp.641-651
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• 2022

The biodiversity research of barley, a functional food, is proceeding to conserve germplasms and develop new cultivar of barley to improve its functional effects. In this study, with 25,104 barley germplasms in the National Agrobiodiversity Center, South Korea, the biodiversity index of species was much lower (1.17) than the origins (24.73) because of the presence of a biased species, Hordeum vulgare subsp. vulgare, but the species and origin of germplasms were significantly different with regard to genetic traits. In the clustering analysis based on genetic traits, we found that 97% barley germplasms could mostly be distributed between 1~7 clusters out of a total of 15 clusters; 'normal and uzu type', 'lodging', and 'loose smut' were commonly represented in the 1~7 clusters and some clusters showed specific differences in five genetic traits including 'growth habit'. In correlation of each genetic trait, the infection of 'barley yellow mosaic virus' was highly correlated to 'number of grains per spike'. '1000 grain weight' was weakly correlated with seven genetic traits including 'number of grains per spike'. Our analysis for barley's biodiversity can provide a useful guide to the species' phenotypes that need to be collected to conserve biodiversity and to breed new barley varieties.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.5
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• pp.514-520
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• 1996

To clarify the proper nitrogen application level for rasing rice infant seedling under different nursery soil, Dongjinbyeo was raised at seedling box with different basal nitrogen level. The results are as follows. The higher the nitrogen level was, the lower emergence rate and the lower establishment rate. The establishment rate was less than 90%, when the nitrogen was more than 2g/box in hill soil and more than 1g/box in paddy soil. The more the nitrogen level was, the higher the seedling height in hill soil, but was higher in the order of N-2, 3, 1 and 0g /box. Leaf number wasn't significantly different between nusery soils and among nitrogen levels when seedling was raised more than 6 days. The endosperm survival rate was decreased as the increased nitrogen level, but wasn't different between the nursery soils. Amount of root was decreased as the increased nitrogen level in paddy soil, but was heavier in the order of N-1, 0, 2 and 3g /box in hill soil. Mat formation was better as the nitrogen level was decreased in all nursery soils. Considering the emergence rate, seedling charactors and mat formation, the proper nitrogen levels seemed to be 2g /box for hill soil and 1g /box for paddy soil.

• Korean Journal of Plant Taxonomy
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• v.52 no.1
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• pp.54-63
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• 2022

Prunus subg. Cerasus is the most planted street and landscaping tree in South Korea, but it is difficult to identify species according to their macro-morphologies, leading to problems when attempting properly to manage species quantities. The purpose of this study is to understand the current status of plant types and species compositions in Bundang Central Park in Bundang-gu of Seongnam City and to discuss the necessity of the management of landscaping tree planting. In April of 2021, during the cherry blossom season, a total of 5,866 planted cherry trees were investigated within an area of 6 km² of Bundang-gu in Seongnam City. As a result, 5,744 trees were sorted into eleven taxa, but the remaining 122 trees were not determined due to their complex morphologies. Prunus ×yedoensis Matsum. accounted for the highest proportion (52.1%), followed by P. serrulata Lindl. var. pubescens Nakai and P. jamasakura Siebold ex Koidz. P. ×nudiflora (Koehne) Koidz., a plant native to Jejudo Island, was not found in this survey. In order to help identify cherry trees based on micro-morphologies, an identification key was presented for the eleven taxa planted as major landscaping trees. It is known that cherry trees frequently form interspecific hybrids in nature. In order to prevent a loss of the genetic originality of native species due to hybridization and gene introgression from foreign cherry trees, it is necessary to manage planting species near the habitats of native taxa and track their origins.

• Journal of agriculture & life science
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• v.50 no.2
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• pp.73-82
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• 2016

Best drought tolerance index was determined through statistics analysis and growth appearance of drought tolerant plants was determined by cultivation in pot and sloping land. For determination of best drought tolerant indicators, RD(Resistant dry days), LD(Leaf area), UTR(Unit transpiration), RWC(Relative water content), RWL(Relative water loss), LA(Leaf area), SN(Stoma unmber) and SA(Stoma area) were carried out by correlation and PCA analysis. RWL and UTR were affected on plant drought tolerance according to comparison among six indices for resistant dry days. The PCs axes separated SA, LA, RD and RWC and SN. UTR was negatively correlated with SA, RWL were also negatively correlated with RWC and SN. RWL and UTR were proved best selection indicator for the selection of drought tolerant species. Ulmus parvifolia, Bidens bipinnata, Patrinia villosa, Kummerowia striata, Arundinella hirta, Artemisia gmelini etc. were selected drought tolerant plants. Shoot growth appearance of drought resistant plants was differed pot and sloping land. Shoot growth and leaf number was no significant differences between the pot and sloping land. However, root growth of drought tolerant plants was all the difference between two cultivation. T/R ratio of drought tolerant plants was also found a big difference. T/R ratio of drought tolerant plants in sloping land was lower than that of pot. These results will be served efficiently plant breeding.

• Journal of Life Science
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• v.33 no.11
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• pp.897-904
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• 2023

This study was done to develope genetic markers with the unique characteristics of genes according to the genomic information of Bacillus velezensis K10. B. velezensis K10 maintained a total of 4,159,835 bps, which was found to encode 5,136 open reading frames (orfs). B. velezensis K10 was found to have much more gene migration due to external factors overall compared to standard strain B. velezensis JS25R. In order to discover genetic selection markers, orfs on the genome to be easily induced to gene mutation were surveyed such as recombinase, integrase, transposase, and phage-related genes. As a result of the investigation, 9 candidate markers were isolated with high possibility as genetic selection markers. Although a part in the various origin's areas showed specificities in comparison with homology, the selected markers were all existed in phage-related areas because they were relatively lower homologies in phage-related genes. PCR analysis was done on B. licheniformis K12, B. velezensis K10, B. subtilis, and B. cereus to establish them as inter-species candidate selection markers. As a result, it was confirmed that B. velezensis K10-specific PCR products were formed in a total of 6 primer sets such as BV3 and BV5 to 9. On the other hand, analysis at the subspecies level observed the formation of B. velezensis K10-specific PCR products in 4 primer sets such as BV3, 5, 8, and 9. Among them, since BV5 and BV8 were detected by very specific results, we suggest that BV5 and 8 can be used as B. velezensis K10 gene selection markers at the species and sub-species level.

• Korean Journal of Environmental Biology
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• v.41 no.3
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• pp.229-246
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• 2023

The bitterling (Cyprinidae, Acheilongnathinae) is a temperate freshwater fish with a unique spawning symbiosis with host mussels. Female bitterlings use their extended ovipositors to lay eggs on the gills of mussels through the mussel's exhalant siphon. In the present study, in April of 2020, we investigated spawning frequencies and patterns of three bitterling fish species in host mussel species in the Nakdong River basin (Hoecheon). During field surveys, a total of four bitterling and three mussel species were found. We observed bitterling's spawning eggs/larvae in the three mussel species: Anodonta arcaeformis(proportion spawned: 45.5%), Corbicula fluminea(12.1%), and Nodularia douglasiae (45.2%). The number of bitterlings' eggs/larvae per mussel ranged from 1 to 58. Using our developed genetic markers, we identified the eggs/larvae of each bitterling species in each mussel species (except for A. macropterus): A. arcaeformis (spawned by Acheilognathus yamatsutae), C. fluminea (A. yamatsutae and Tanakia latimarginata), and N. douglasiae (A. yamatsutae, Rhodeus uyekii, and T. latimarginata). Approximately 57.6% of N. douglasiae mussel individuals had eggs/larvae of more than one bitterling species, suggesting that interspecific competition for occupying spawning grounds is intense. This is the first report on bitterling's spawning events in the Asian clam C. fluminea from Korea; however, it should be ascertained whether bitterling's embryo undergoes successful development inside the small mussel and leaves as a free-swimming juvenile. In addition, the importance of its conservation as a new host mussel species for bitterling fishes needs to be studied further.