• Journal of Korean Society of Forest Science
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• v.96 no.3
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• pp.290-294
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• 2007

Interspecific hybrid seedlings by artificial crossing of Genus Quercus (Q. serrata, Q. dentata, Q. mongolica var. crispula, Q. aliena) were planted at nursery in Tottori University. Seedlings of hybrids by natural crossing(Q. fabri and $Q.{\times}mccormickii$) were selected and planted at Hiruzen district in Tottori University. Artificial interspecific hybrid $F_1$ and natural hybrid $F_1$ bloomed when they were 4 years old and 3 years old, respectively. The pollen fertility and seed viability were investigated from the bloomed individuals in 2001. The germination percentages of the pollens of artificial interspecific hybrid were more than 84% except one individual, and the extension of pollen tubes was normal. The normal seed percentages of artificial interspecific hybrid were more than 90% similar to parents. Germination percentages of normal seed of natural crossing family were more than 64%, respectively, and selfed offsprings of Q. fabri, and $Q.{\times}mccormickii$ hade high reproductive ability.

• Korean journal of applied entomology
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• v.32 no.2
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• pp.193-199
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• 1993

The morphological relationship between Drosophila melanogaster and D. simulans, and their hybrids were investigated using comparisons of internal reproductive organs. male sex comb tooth number and male genital discs. InterspecIfic hybrids of all crosses were completely sterile, and had rudImentary gonads. Dysgenic ovaries and testes were morphologically similar to those of gonadal dysgenesis stenlity associated with the PM system in D. melal1ogaster. The mean number of sex comb tooth in D. melrmogaster, D. simulans and their male hybrids were 10.73, 8.35 and 9.97, respectively. The general aspects of the genital disc of the malo hybrid were a mosaic-like structure between D. melanogaster and D. simulans.

• Korean journal of applied entomology
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• v.34 no.4
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• pp.308-313
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• 1995

Drosophila melanogaster, D. simulans, and D. sechellia are closely related species which belong to the D. melanogaster complex; the first two cosmopolita and the last one restricted to th Seychelles archipelago. The phenogenetical relationship between this complex and their hybrids were investigated by the comparison of sex-comb tooth number an genital arch of male. In interspecific hybrids of all crosses between three species four hybrid males were produced and completely sterile. Males of D. simulans (${O}_{9}$) have significantly less sex-comb teeth (mean 8.35) than either D. melanogaster (OR, mean 10.73) and D. sechellia (Ja, mean 10.60). From the analysis by the number of sex-comb tooth in interspecific hybrids we could not represent the direction of heredity nature. each species of D. melanogaster complex were characteristic in the shape of the genital arch, which readily allows these species to be distinguished. The common structure of the genital arch in the interspecific hybrids were mosaic-like structure between parental species.

• Korean Journal of Plant Tissue Culture
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• v.25 no.2
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• pp.135-139
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• 1998

Reciprocal interspecific hybrids between N. glauca(2n=24) and N. langsdorffii(2n=18) were obtained by intercrossing. One hundred percent of F$_1$ seeds was produced from intercrossing of N. glauca $\times$ N. langsdorffii, whereas the frequency of F$_1$ hybrid seed formation from N. langsdorffii $\times$ N. glauca was very low. However, all the hybrid seeds were germinated well and then grown to normal plantlets. All the plants of F$_1$ hybrids have chromosome number of interspecific hybrids (2n=21). From observation of morphological characteristic, the structure of petrol, leaf, flower, and the morphology of pollen have characteristics of F1 hybrid. Spontaneous tumors (genetic tumor) were formed from each F$_1$ hybrid; the genetic tumor arose at the reproductive phase when the maternal type of F$_1$ hybrid came from N. glauca, while the genetic tumor arose only after reproductive phase when the maternal type of F$_1$ hybrid came from N. langsdorffii. The genetic tumor actively proliferated on hormone-free medium and produced numerous teratoma shoots. In addition, normal leaf or stem explants of F$_1$ hybrid produced calli on hormone-free medium after 15 days of culture, the calli produced new numerous teratoma shoots after 30 days. The frequency of teratoma shoot formation from rnterspecific hybrid was higher in the N. glauca $\times$ N. langsdorffii than in the N. langsdorffii $\times$ N. glauca. Root development from the teratoma shoots was hardly obtained. Teratoma shoots without roots in vitro can form genetic tumor at the vegetative growth phase after tissue culture.

• Korean Journal of Ichthyology
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• v.22 no.4
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• pp.225-229
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• 2010

A intergeneric hybrid between Rhodeus uyekii and Acheilognathus signifer was collected in the Jojongcheon Bukhan River. The hybrid showed intermediate characteristics of two parental types in some key characters, such as color pattern of body, pigmentation of dorsal, anal, and caudal fins, and body spot and stripe. In addition, the hybrid had a fairly large number of lateral line scales, while it lacked a barbel, which is known as a key intergeneric character. The probable occurrence of a natural hybrid is supported by a viable $F_1$ hybrid produced with the parental species.

• Journal of Aquaculture
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• v.9 no.2
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• pp.133-140
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• 1996

Hybird and allotriploid between female rainbow trout (Oncorhynchus mykiss) and male coho salmon (O. kisutch) were produced by artificial fertilization and heat shocks. Hatching and survival rate of allotriploid at 2 month after hatching was $77.6\%$ and $54.5\%$ respectively, and these rates clearly exceeded those of their hybrid. Cell and nuclear sizes of the erythrocyte of hybrid were intermidiate of their parents and those of allotriploid were larger than thier hybird. The somatic chromosome number of viable hybrid was 2n = 60 and that of allotriploid was $90\~93$ with chromosomal polymorphism. Allotriploid karyotpe was constituted by two sets of rainbow trout chromosome and one set of coho salmon chromosome.

• Journal of Korean Society of Forest Science
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• v.29 no.1
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• pp.102-114
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• 1976

This experiment was conducted to investigate the percentage of fertile seed in terms of crossabilities and relationships of taxonomic affinities for the ${\times}$ P. rigitaeda of interspecific hybrid, ${\times}$ P. rigida rigitaeda and ${\times}$ P. rigitaeda rigida of backcross hybrids, $F_2$ of ${\times}$ P. rigitaeda and natural hybrid of ${\times}$ P. rigitaeda within Sub-genus Diploxylon of the Genus Pinus. The possibility of establishment of hybrid seed orchard and differentia of hybrids for the purpose of extensive program of reforestation in the future have also been investigated. And, the experimental results obtained are summarized as follows: 1. On the basis of crossabilities as well as on the taxonomic affinities according to the systems of Shaw, Pilger and Duffield, it has been proven that the parental species of those hybrids are of close affinities and range of the fertile hybrid seed production rate was as high as 67-87% in the best hybrid combination (Table 6). 2. Those hybrids seemed to be most promising in the growth perfermance exhibiting 28-80% more volume growth compared to the P. rigida with the statistic significance of 1-5% level (Table 7, 8, 9). And all hybrids exhibit cold hardiness as much as P. rigida except $F_1$ hybrid of ${\times}$ P. rigitaeda and it seems to suggest that the characteristics of cold hardiness were transmitted from the P. rigida. 3. With regard to the anatomical characteristics of needle, the hypoderm is biform in most of the hybrid pines and the characteristics of resin canals are medial in all hybrid. And, the fibrovascular bundles are intermediate of both parent in all hybrid. Therefore it was found to be possible to distinguish the hybrids pines from their parents by the needle characteristics (Table 10). 4. It has been demonstrated that the hybrids pines have a phenolic substance (No. 7) of pale yellow at Rf-0.66, same as P. rigida, but no trace of phenolic substance was observed in the P. taeda. This fact will serve as an important criteria for early identification of hybridity in progeny testing (Table 11). 5. It was found to be possible to distinguish by the starch gel electrophoretic variations banding patterns and staining densities of isoperoxidase in the needles of the hybrids pines from their parents (Fig. 1).

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.11a
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• pp.200-201
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• 2000

• Korean journal of applied entomology
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• v.35 no.3
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• pp.216-220
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• 1996

Drosophila simulans and D. mauritiana are sibling species, the former cosmopolitan and the latter restricted to the oceanic island of Mauritius. Sex comb-tooth number of male flies of D. simulans were about 9.83, while those of D. mauritiana were 12.90. Genital arch of D. simulans is large semicircular shaped expasion, while that of D. mauritiana is a narrow fingerlike expansion. We used classical genetic analysis to measure effects of genes on the X chromosome responsible for numeral and morphological differences in sex comb-tooth and genital arch between these species, respectively. For these purposes, mutant strain of D. simulans and wild type strain of D. mauritiana were hybridized and males of the FI and the backcrossed progenies were compared with two characters above mentioned. The sex comb-tooth number of F, males were about 11.79, and the genitalia of F, male were intermediate in shape between those of D. simulans and D. mauritiana. Genetic analysis of sex comb-tooth number and genital arches differing between D. simulans and D. mauritiana showed that very little diffemce was due to effect of the X chromosome.

• The Korean Journal of Zoology
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• v.35 no.3
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• pp.339-343
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• 1992

버들치, 버들개의 공서지역에서 2종 사이의 종문제를 명확히 구명하기 위해 883개체를 채집하여 형태형 질과 유전자분석을 실시하여 생식적 격 리기작 여부를 조사한 결과는 다음과 같다. 2종 사이 에 잡종개체가 약 12%( n = 104) 발견되었다. 형 태분류형 질인 측선상부린수( SAL)의 경우 버들개와 버들치가 각각 SAL = 24.25$\pm$1, 32, 16.93 $\pm$ 0.92로 뚜렷한 차이를 나타내었으나 잡종개체는 평균 SAL = 19.56$\pm$1.80으로 중간값을 나타내었다. 잡종개체 중 F2 이상의 잡종개체 비율은 약 2.7%로 Fl(9"yp)에 비해 현저히 낮고, 잡종개체의 출현률이 기 대치보다 낮은 것으로 보아(P < 0.001) 2종간에 자유교배가 되지 않으며 따라서 Predating isolating mechanism이 작용한다고 보며 또한 잡종개체의 치사율이 높은 점으로 보아 이들사이에는 Postdating isolating mechanism도 동시에 작용한다고 사료되며 따라서 이들 2종은 비교적 최근에 분화된 별종임이 재확인 되었다. 되었다.