• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.221-225
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• 2009

Interspecific crossing was conducted to obtain hybrid lilly with better quality of adapting to the unfavorable environment using oriental Lilium spp. as a female parent. Average interspecific crossing rate between oriental and asiatic lines by cut-style pollination was as low as 29%. Although the average germination rate of zygotic hybrid embryo between oriental 'Rodolfa' ${\times}$ asiatic 'Toronto' was increased from 76.6% to 78.3%. on 114 or 1/6 strength MS medium compared to the control, the rate of zygotic embryos formation between oriental 'Farolito' ${\times}$ asiatic 'Buff Pixie' was significantly enhanced up about 86~90% on 1/2 strength MS medium. Meanwhile, germination rate of interspecific hybrid embryo between oriental 'Snow Cristal' ${\times}$ asiatic 'Royal Trinity' showed up 100%. Germination rate of interspecific hybrids slightly increased by addition of $0.5-1.0mg{\cdot}L^{-1}$ GA. Germination rate of zygotic embryos between oriental 'Farolito' ${\times}$ asiatic 'Buff Pixie' and oriental 'Belcanto' ${\times}$ L. callosum was highest on the MS medium containing $0.01mg{\cdot}L^{-1}$ NAA and $0.1mg{\cdot}L^{-1}$ kinetin, as 66.6% and 45.2% respectively.

• Korean Journal of Agricultural Science
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• v.26 no.2
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• pp.39-48
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• 1999

This study was carried out to investigate the difference and genetic similarity at the level of molecular genetics. Genomic DNA was extracted from blood of Holstein, Korean cattle, Charolais, and hybrid between Korean cattle and charolais and RAPD(random amplified polymorphic DNAs) was analyzed by PCR(polymerase chain reaction). After genetic similarity value from different breeds are analyzed, genetic similarity was estimated by UPGMA(unweighted pair-group method using average). The results obtained from this study can be summarized as follows: 1. When genomic DNA which was extracted from different breeds was subjected to electrophoresis on 1.5% agarose gel, bigger than 12.2kb was appeared. Ratio by absorbance of $A_{260}/A_{280}$ was 1.75~2.10, indicating that genomic DNA was quite pure for RAPD analysis. 2. Different band patterns by RAPD were appeared according to the breeds in cattle. The best primer used to distinguish Holstein from other breeds was 5'-GAC CGC TTG T-3'. 3. A 340bp fragment was amplified in $33.0^{\circ}C$ of annealing temperature for the Holstein and Charolais breeds, but any amplification was not occurred in this annealing temperature for Korean cattle and hybrid. In addition, a 340bp fragment was amplified in $37.5^{\circ}C$ of annealing temperature for the Holstein and Korean cattle, but any amplification was not occurred in this annealing temperature for Charolais and hybrid. For the reaction of PCR. $37.5^{\circ}C$ and $33.0^{\circ}C$ of annealing temperature was shown to be best for genetic marker identification from Holstein, Charolais, and hybrid between Korean cattle and Charolais. 4. When genetic similarity from different breeds are analyzed at the both temperature of $33.0^{\circ}C$ and $37.5^{\circ}C$, the genetic similarity value of Holstein and Korean cattle, Holstein and Charolais, Korean cattle and Charolais, and Korean cattle and hybrid were 0.666~0.777, 0.615~0.666, 0.400~0.461 and 0.857~0.888, respectively. 5. It could be concluded that different breeds are capable of distinguishing by RAPD used random primer 5'-GAC CGC TTG T-3', genetic similarity from different breeds was appeared the higher genetic similarity value of Korean cattle and Charolais than that of Holstein between Korean cattle and Charolais by UPGMA.

• The Korean Journal of Zoology
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• v.24 no.4
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• pp.201-215
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• 1981

In vitro intra- and interspecific molecular hybridizations of lactate dehydrogenase isozymes from six species were performed by freezing and thawing the isozymes in sodium chloride, resulting each of the interspecific hybridization of Fluta alba $H_4 \\times$ Ophicephalus argus $M_4$ and that of O. argus $M_4 \\times$ Parasilurus asotus isozymes showed one hybrid isozyme considered to be an $M_2H_2$ isozyme. The results fit with the suggestion that the isozyme of lower vertebrates is a dimer of homodimer as far as te assemblies of subunit M and subunit H are concerned.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.4
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• pp.288-294
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• 1994

Seedling vigor of introgressive hybrids between birdsfoot refoil 'MO-20"(Lotus corniculatus) used as the maternal parent and tetraploid(2n=4x=24)accession PI302921 narrowleaf trefoil(Lotus corniculatus L.), and parents were compared in terms of dry matter accumulation , net photosynthetic rate, dark respiration rate, and growth analysis. The hybrids showed better seedling vigor than PI302921 as they had lower dark respiration rate and higher relative growth rate (RGR) than PI302921. Hybrids grew faster than MO-20, but there was not significant difference between them. Net photosynthesis per unit area increased during three weeks after emergence without difference among the entries, then decreased sharply with leaf age and shading marking much differences among entries Dark respiration rate was very high just after emergence, after which it decreased rapidly until it reached a stable level at 1 week through 3 weeks of age, and then dropped again. In general, dark respiration, photosynthesis, and seedling growth of the hybrid was similar to those if MO-20. Although there are some reports about interspecific hybrids of Lotus species, it still remains as a new area for genetic improvement of seedling vigor of L. corniculatus.atus.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.49-49
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• 2020

본 연구는 호랑버들과 갯버들 교잡종의 잎 형태적 특성을 국내 자생종인 호랑버들, 갯버들과 비교하였다. 호랑버들 35개체, 갯버들 35개체, 교잡종 100개체에서 잎을 채취하여 잎의 길이와 너비, 엽저와 엽두 각도, 잎자루 길이와 너비, 탁엽의 길이와 너비, 잎 두께 등 총 17개의 잎 형태적 특성을 분석한 결과, 엽두 각도와 탁엽의 길이를 제외한 15개의 특성에서 세 분류군이 유의한 차이를 나타내었다. 교잡종의 잎 길이와 너비의 비는 갯버들, 교잡종, 호랑버들 순으로 크게 나타나 교잡종의 잎의 형태는 피침형인 갯버들과 타원형인 호랑버들 사이 중간 형태로 나타났다. 교잡종의 잎의 너비, 엽저 각도, 잎자루의 길이와 너비, 탁엽의 길이와 너비는 호랑버들과 갯버들의 중간 형태를 나타내었으며, 탁엽의 거치 개수는 교잡종이 호랑버들과 갯버들보다 많은 특성이 나타났다. 잎 뒷면 단위면적당 털의 개수 또한 교잡종이 호랑버들과 갯버들보다 많은 특성을 나타냈다. 세 분류군 간 잎 형태적 차이를 구명하기 위해 유의한 차이를 보인 15개의 변수를 이용하여 주성분분석을 실시한 결과 고유값이 1 이상인 제 1, 2, 3, 4주성분이 전체 분산의 68.2%를 설명하였다. 제 1주성분은 잎의 너비, 잎의 1/3 아랫부분 너비, 잎의 1/3 윗부분 너비, 잎자루의 길이, 잎의 길이로 잎의 크기와 관련이 있는 특성들이 높은 상관관계를 나타내었고, 제 2주성분은 엽맥의 수, 잎자루의 너비와 높은 상관관계를 나타내었다. 제 3주성분은 탁엽의 거치 개수, 탁엽의 너비로 탁엽의 특성과 관련이 있는 특성들이 높은 상관관계를 나타내었으며, 제 4주성분은 잎의 두께와 높은 상관관계를 나타내었다. 주성분분석 결과 호랑버들과 갯버들 교잡종의 잎 특성은 호랑버들보다 갯버들과 가까운 것으로 나타났다. 잎의 형태적 특성으로 세 분류군을 구별하기 위해 정량형질을 이용한 판별분석 결과 교잡종 중 대부분(93.3%)이 호랑버들, 갯버들과 구별되었다. 일부 교잡종 개체(6.7%)가 갯버들로 오분류되었지만, 잎의 정성적 특성인 잎의 거치와 측맥의 특성을 고려한 결과 모든 개체가 교잡종으로 구분되었다. 따라서 호랑버들과 갯버들의 교잡종은 잎의 형태적 특성이 부모종인 호랑버들, 갯버들과 다른 독특한 형태를 가진 것으로 나타났다.

• Korean Journal of Ichthyology
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• v.5 no.1
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• pp.113-121
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• 1993

Cytogenetic studies and hybridization experiment using two populations of Oryzias latipes from Korea were conducted in order to examine their systematic positions. In the chromosome number of specimens examined. O. sinensis had 46 with a pair of large metacentrics and O. latipes had 48 chromosomes without a pair of large metacentrics. However, the diploid chromosome number of the hybrid between O. latipes and O. sinensis was 47 and they had a large metarcentric chromosome in their karyotype. In the $F_2$ hybrids, distribution of chromosome number was variable among individuals. Hatching rates showed little difference between control and hybrids. However, hatchiabilties of $F_2$ hybrids were decreased by 83.3% as compared with $F_1$ offspring. Incidence of abnormal larvae was increased by 15.3% as compared with their parents. These results indicate that the two karyotype populations formerly regarded as two subspecies would be two distinct allopatric sibling species O. sinensis, and O. latipes.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.320-323
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• 2009

A new grafting cactus 'Yeomyeong' was developed by an interspecific crossing between Gymnocalycium marsoneri and G mihanovichii at the National Institute of Horticultural and Herbal Science, Rural Development Administration in 2005. In 2001, an interspecific crossing line '9922012' with orange color was selected from the cross of G. marsoneri ${\times}$ G. mihanovichii 'IG-112'. The line '9922012' as a female parent was crossed with G. mihanovichii 'IG-177', and the orange colored lines were obtained in 2002. The 'Wonkyo G1-159' was selected from the orange colored lines through three times of characteristic evaluation during 2003 to 2005. The 'Wonkyo G1-1 59' was named 'Yeomyeong' in the RDA in 2005. The color of both body and tubercle was orange. The shape of globe was flattened round and it had 7 to 9 ribs. The spine was erect, short sized and grayish brown color. Growth was faster, reaching 41.8-47.7 mm in diameter, than the comparison cultivar 'Hwangwol', at 10 months after planting. The number of tubercle was 6.9-12 per globe. 'Yeomyeong' was developed for export to the America and the Netherlands. Characteristics of the cultivar could be maintained by grafting on the Hylocereus trigomus.

• Korean Journal of Plant Resources
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• v.32 no.4
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• pp.296-302
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• 2019

This study was accomplished to select breeding materials among the pear germplasm 6 species 43 accessions and check out the expression of scab incidence of inter specific hybrids of 5 crosses 609 seedlings for scab resistance breeding. Fruitlets scab incidence of P. pyrifolia, P. bretschneideri and P. ussuriensis were 59.5, 73.4 and 63.0% respectively, and also fruitlets scab incidence of interspecific hybrid cultivars between P. pyrifolia and P. bretschneideri were 77.7%. However, fruitlets scab incidence of P. communis and interspecific hybrid accessions between P. pyrifolia and P. communis were significantly less than 15%. As a result of the Duncan test, scab resistance of pear accessions were classified into two groups: P. communis and their hybrid were resistance and P. bretschneideri, P. pyrifolia, P. ussuriensis, and among their hybrid were susceptible. Also, it showed the same trend in the seedlings. Scab incidence of 'Seolwon' ${\times}$ 'Chuwhangbae' and 'Supergold' ${\times}$ 'Mantienhong' in P. pyrifolia seedlings showed more than 80%, while that of interspecific hybrid between P. pyrifolia and P. communis showed about 2%. It showed scab resistance of P. communis transferred to hybrid seedlings and expressed to be dominant in their seedlings. Namely, P. communis was good breeding materials for scab resistance.

• Korean Journal of Poultry Science
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• v.41 no.1
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• pp.39-44
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• 2014

The goal of this study was to evaluate the growth performance of crossbred strains using Korean native chicken (KNC). The data were collected from 2012 through 2013 at Poultry Science Division, National Institute of Animal Science, Korea. The number of chicks analyzed in this study was 375. Crossbred were (A) $R{\times}S$, (B) $D{\times}H$, (C) $D{\times}S$, (D) $C{\times}Y$ and (E) $Y{\times}H$. The fertility rates of strains were 94.9% in crossbred A, 91.9% in crossbred B, 91.8% in crossbred C, 89.7% in crossbred D and 93.8% in crossbred E, respectively. The fertility was highest in crossbred A, but crossbred C showed the lowest (p<0.05) based on the hatchability. The crossbreds B and C exhibited a superior performance on body weight gains during growing stages but crossbred D showed the lowest (p<0.05). The feed intake was to the pattern of body weight. The feed intake of crossbred D was significantly lower than other crossbreds. Also, feed conversion ratio of crossbred D showed the lowest (p<0.05). Egg production ratio of crossbred D was significantly higher compared to the other crossbreds. These results suggest that the development of new crossbreds of commercial Korean Native Chickens should be required for better performance.

• Journal of Mushroom
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• v.17 no.4
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• pp.224-229
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• 2019

The two most common mushroom species grown in Korea are pearl oyster mushroom (Pleurotus ostreatus) and king oyster mushroom (P. eryngii). In recent years, the production of king oyster mushroom greatly increased due to the automation of the cultivation facilities, and it became a major export mushroom owing to its excellent shelf life. However, the increase in the production of king oyster mushroom led to a decline in its market price; thus, necessitating the development of new mushroom species that could replace king oyster mushroom, to diversify the mushroom market for the benefit of both, the producers and the consumers. The Mushroom division at the National Institute of Horticultural & Herbal Science (NIHHS) reported the development of a new interspecific hybrid between P. ferulae and P. tuoliensis, referred to as 'Creamy.' Two parental strains KMCC00430 (Bisan2ho, P. ferulae) and KMCC00461 (P. tuoliensis) were selected based on the results of genetic resource analysis, and their monokaryons were collected. About 1,000 Mon-Mon crosses were performed and 73 of them were selected. Following repeated cultivation tests and strain analyses, we selected strain 7773, which had a bright creamy pileus and a thick straight stipe, and named it 'Creamy.' Optimum temperature for mycelial growth of Creamy was 25-30℃, and that for fruiting body growth was 16℃. The pileus, which had a brighter creamy color, was small in size with a diameter of 61.2 mm. Although it was cultivated in suboptimal conditions, such as low temperature and high CO₂ concentration, Creamy was characterized by its straight and smooth stipe. Field production tests and further analyses indicated that the yield of Creamy was 5% higher than that of Baekhwang. It is expected that Creamy, the new interspecific hybrid with a bright creamy pileus and a pleasant flavor, will help create new opportunities for mushroom farmers and diversify the mushroom market.