• Clinics in Shoulder and Elbow
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• v.12 no.1
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• pp.94-97
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• 2009

Purpose: Hibernoma is a very rare benign soft tissue tumor of the hypervascularized brown fat tissue with no malignant potential. Materials and Methods: However, it is difficult to differentiate a hibernoma from other malignant tumors, such as liposarcoma using computed tomography and magnetic resonance imaging, and a surgical resection with histological confirmation is the treatment of choice. Results: Histopathologically, hibernoma is composed of brown adipose cells that are polygonal and multivacuolated with a centrally located nucleus and granular cytoplasm, unlike white adipose cells. Conclusion: This article describes a patient with a histologically-confirmed hibernoma of the right shoulder.

• Journal of Chest Surgery
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• v.35 no.2
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• pp.94-101
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• 2002

All kinds of tissue valves must be pretreated for the inactivation of immunologic properties and the strengthening of tissue before implantation. However, the tissue valves are gradually denatured with the calcification process and they eventually lose their functions. Recent reports have shown the existence of specific calcium binding non collagenous proteins in the calcified area of implanted biomaterials. This experiment was intended to confirm the effect of pretreatment with RGDS(Arg-Gly-Asp-Ser) tetrapeptide on the calcification of subcutaneously implanted bovine pericardium in rats. RGDS tetrapeptide has the same amino acid sequence of attachment site of specific calcium binding non collagenous proteins. Material and Method: All bovine pericardial pieces were fixed with 0.6% glutaraldehyde. The pretreatments were done using 5 different methods, groupI, with normal saline for 60 minutes, groupII, with 0.5% GRSD(Gly-Arg-Scr-Asp) tetrapeptide solution for 60 minutes, group III : with 0.5% RGDS(Arg-Gly-Asp-Ser) tctrapeptide for 30 minutes, group IV ; with 0.5% RGDS for 60 minutes, and group V : with 0.5% RGDS for 120 minutes. The pretreated bovine pericardial pieces were implanted subcutaneously at the abdominal sites of rats. 30 days after the implantation, the implanted bovine pericardial tissue were examined radiologically, biochemically, and histologically to measure the severity of calcification. Result: On the radiological examination, group I ; 68.42$\pm$3.06, group II , 64.25$\pm$5.58 showed significant difference with group III: 48.00$\pm$3.57, group IV; 43.67$\pm$2.31, and group V ; 2.58$\pm$2.47(p<0.05). There was no difference between group I and II(p=0.105). On the biochemical examination, the amount of calcium in group I was , 33.09$\pm$6.59 mg, in group II ; 28.12$\pm$5.50mg, in group III ; 25.42$\pm$7.67mg, in group Ⅵ ; 20.51$\pm$5.11mg, and in group V : 15.43$\pm$4.25mg.

• Journal of Aquaculture
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• v.17 no.1
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• pp.24-29
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• 2004

The high mortality of cultured turbot, Scophthalmus maximus was occurred from Gochang in June, 2003. Infect fish with iridovirus showed a lower feed intake and lethargic. These fish exhibited pale body color, extended abdominal and exophthalmus. Histopathological studies showed basophilic enlarged cells from kidney, spleen, gill, heart, muscle, stomach, intestine, liver and pancreas of these fish. In PCR amplification with red sea bream iridovirus (RSIV) specific primer set of a DNA fragment of 286 bp was obtained from infect turbot with the virus. The strain showed the high homologies with RSIV, LBIV (largemouth bass iridovirus), GSIV (giant seaperch iridovirus) and SBIV (sea bass iridovirus).

• Proceedings of the Materials Research Society of Korea Conference
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• 2009.05a
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• pp.46.2-46.2
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• 2009

In vitro 상에서 골조직을 원활하게 재생하기 위해서는 3차원 지지체를 이용한 세포 배양과 세포 배양 시 세포의 형태와 기능을 유지/향상시키기 위한 인체 내 미세 환경 재현은 필수적이다. 따라서 본 연구에서는 뼈 성분과 유사한 생체 활성 물질인 hydroxyapatite (HA)와 생분해성 고분자인 poly $\varepsilon$-caprolactone (PCL)를 복합재료로 이용하여 내부 연결성이 우수한 골조직 재생용 3차원 지지체를 제작하였으며, 골 재생 능력 향상을 위하여 인체내 골조직의 기계적 미세 환경을 체외에서 구현한 새로운 형태의 perfusion bioreactor system을 개발/적용하였다. 또한 본 연구에서 개발된 perfusion bioreactor system의 생물학적 평가를 위해 MG63 (osteoblast like cell, 한국 세포주 은행)과 New Zealand White Rabbit에서 분리한 중간엽 줄기세포를 골조직 재생용 3차원 지지체에 파종하였다. 48시간 동안 안정화 후 perfusion bioreactor system을 이용하여 기계적 자극을 파종된 세포에 인가하였으며, 배양 기간 동안 세포의 증식 확인 및 형태학적 관찰을 실시하였다. 본 연구 결과, perfusion bioreactor system을 이용하여 기계적 자극을 인가한 실험군에서 세포의 증식 및 활성도가 대조군에 비해 우수함을 확인 할 수 있었다. 따라서, perfusion bioreactor를 이용한 세포 배양은 세포의 활성 향상 및 골조직 재생에 도움이 될 것으로 사료된다. 차후 perfusion bioreactor를 이용한 다양한 패턴의 자극이 골재생 능력 및 중간엽 줄기세포의 골 분화능에 미치는 영향에 대한 연구가 필요할 것으로 사료된다.

• Progress in Medical Physics
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• v.12 no.1
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• pp.79-94
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• 2001

The fluorescence emanating from a biological tissue contains information about scattering, absorption and the intrinsic fluorescence (fluorescence only due to fluorophores). Becaue fluorescence spectra of biological tissue are often significantly affected by the presence of tissue absorbers and scatterers, the measured tissue fluorescence cannot be interpreted as a linear combination of intrinsic fluorescence spectra of different tissue biochemical. We conducted experiments to examine the influence of scattering and absorption on the experimentally measured fluorescence of a turbid medium such as biological tissue. Therefore, we acquired fluorescence and reflectance spectra of tissue phantoms with a wide range of scatterer and absorber concentrations. By applying a photon migration model, which uses the scattering and absorption information contained in reflectance spectra to remove their distortion also present in fluorescence spectra, we extract the intrinsic fluorescence of these tissue models. We achieved excellent agreement between modeled and actual intrinsic fluorescence spectra. The motivation for this research is that intrinsic fluorescence spectra are expected to change with progression of disease in human tissue, due to changes in the tissue biochemical composition. It is not possible to distinguish the two tissue types by using only the measured fluorescence, however clear separation can be achieved with the intrinsic fluorescence in real time optical biopsy.

• Journal of Periodontal and Implant Science
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• v.28 no.4
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• pp.577-604
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• 1998

chitosan은 골치유증진 및 골세포의 분화를 촉진하는 것으로 알려진 천연의 생분해성 고분자이다. 이연구에서는 chitosan 및 chitosan/tricalcium phosphate(TCP) 다공성 기질을 제조하여 골이식재 및 조직공학적 골형성을 위한 3차원적 세포배양 지지체로서의 가능성을 평가하고자 하였다. chitosan 용액 및 TCP가 포함된 chitosan 용액을 동결건조함으로써 소공의 크기가 $100-200{\mu}m$인 스폰지형태의 chitosan 및 chitosan/TCP 다공성 기질을 제작하였다. 골이식재로서의 효과를 평가하기 위하여 백서의 두개골 결손부에 제작된 chiosan 및 chitosan/TCP 다공성 기질을 각각 이식하고 2주 및 4주 후에 동물을 희생하여 조직학적으로 치유양상을 관찰하였다. 조직공학적 골형성을 위한 세포배양 지지체로서의 가능성을 평가하기 위하여 백서 태자의 두개골에서 분리된 골아세포를 chitosan 및 chitosan/TCP 다공성 기질에 각각 접종하고 56일간 배양하면서 각 기간 별로 세포수, 염기성 인산효소 활성, 축적된 calcium의 양을 측정하였고 배양된 세포-기질 혼합체를 광학현미경 및 주사전자현 미경하에서 조직학적 관찰을 시행하였다. 백서 두개골결손부에 이식된 chitosan 및 chiosan/TCP 다공성 기질은 별다른 이물반응 없이 자연 분해되면서 신생골조직 내에 매립되었으며 이식하지 않은 대조군에 비해 유의하게 높은 신생골형성 효과를 나타내어 우수한 골전도성이 있음이 확인되었다. 신생골형성 양상이나 형성된 양에 있어서 두 가지 기질간의 유의한 차이는 없었다. 골아세포-기질 혼합체의 배양결과, 접종후 배양 28일 경과 시까지 골아세포수는 지속적으로 증가하다가 이후에는 5 8일까지 성장정도가 둔화되었다. 염기성 인산효소의 활성 및 calcium 축적량은 접종후 배양시간경과에 따라 56일까지 지속적으로 증가하였다. 세포수 및 염기성 인산효소의 활성에서 두 기질간의 유의한 차이는 없었고, calcium 축적량에 있어서는 chitosan/TCP 기질에서 유의하게 높았고 증가속도도 컸다. 배양된 골아세포가 접종된 다공성 기질의 조직학적 관찰결과, 골아세포는 다공성 기질에 잘 부착하여 중층의 형태로 성장하면서 광화된 골기질을 형성함이 관찰되었다. 배양 14일부터 작은 골편형태의 골형성이 기질 표면에 부착되어 관찰되었고, 배양기간이 길어짐에 따라 성장하여 배양 56일째에는 상당한 양의 광화된 골질이 형성됨이 관찰되었다. 배양 56일 경과후의 광화된 골질의 양은 chitosan/TCP 기질에서 더 많았다. 이 연구의 결과, chitosan 및 chitosan/TCP 다공성 기질이 골이식재로서 뿐만 아니라, 조직공학적 골형성에 적용되는 골아세포의 배양을 위한 3차원구조의 세포지지체로 이용되어 골재생술식에 유용한 생체재료로 활용될 수 있음이 확인되었다.

• Applied Microscopy
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• v.36 no.3
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• pp.183-194
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• 2006

The threadlike structures of fascia were examined by tight and electron microscopy. In order to distinguish its tissue organization, we used staining methods including hematoxylin-eosin, Masson's trichrome, Van Gieson's collagen fiber stain and Kluver-Barrera's luxol fast blue for nerve stain. Under the light microscope, the threadlike structures were composed of many collagen fibers and nerve. In higher magnification, they looked like as the bundle of tubular structures. Many myoid cell-spindle nuclei were observed in the tissue, which were taken from the fascia. It was identical with Bonghan duct known as one of meridian network theory. In the early 1960's the North Korean Bong-Han Kim showed the anatomical structures of the acupuncture points, and explained the meridian system as the concrete duct network system. According to Bonghan theory the Bonghan ducts spread throughout the body Because it is believed that the duct could have the role of signal pathway, the theory was reinvestigated in these days. All of the threadlike structures isolated from fascia shows the abundance of collagen fibers. The electron microscope examination (TEM) could confirm the well arranged collagen fiber and nerve. This investigation reveals that superficial Bohghan duct are nerve fiber parallel running with collagen fibers. We conjectured that the intermingled structure of collagen fiber, blood vessel and nerve fiber might have the role of meridian system. And the more, regardless of histological research, the study on collagen fiber as response transmitter in acupuncture treatment are in need.

• Journal of Veterinary Clinics
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• v.29 no.2
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• pp.173-176
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• 2012

A 6-year-old male Shih tzu dog was presented for evaluation of abdominal distention. Abdominal radiography and ultrasonography revealed a soft tissue density mass containing large amount of fluid. Ultrasonography-guided fine needle aspiration of the mass was performed and cytologic impression was granulation tissue with hematoma and fibroplasias. On exploratory laparotomy a mass was identified at the root of mesentery adhered to distal jejunum. Because the mass could not be separated from the attached jejuna loops, the mass and the adhered sites were surgically removed all together and enteroanastamosis was performed. Histologically a low grade myxosarcoma was diagnosed. Tumor cells were positive with alcian blue stain and Ki67 index by immunohistochemistry was 2.5. The dog recovered from surgery uneventfully, and has been in good condition without any signs of recurrence or metastasis for about 30 months after surgery.

• Ocean and Polar Research
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• v.36 no.2
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• pp.121-134
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• 2014

Sessile marine bivalves including mussels, oysters and clams are often used as a sentinel species in coastal environmental monitoring since changes in the environmental quality are often well preserved in their tissues and shells. In this study, we investigated overall health condition of the Mediterranean Mussel, Mytilus galloprovincialis on the south coast using histology. Reproductive condition as gonad index (GI), condition index (CI) as a ratio of the tissue weight to the shell weight, digestive gland atrophy (DGA), types of parasites, and pathologic conditions including erosion, necrosis, hemocyte infiltration, and neoplasia were examined from each histological preparation. GI decreased from March to July then increased from July to September and spawning mussel could be observed as early as in April and the activity continued until September. CI also followed the monthly changes in GI, indicating that decrease in CI was associated with the weight loss due to spawning. DGA increased from March to June, decreased in July and increased from July to September. High DGA values observed in June and September were coincided with spawning and high water temperature. Histology also showed high prevalence of erosion in the digestive gland in June (36.0%) and September (56.4%), suggesting that high water temperature and spawning acted as environmental stressors. No parasitic organism was identified during the survey, although some symbiotic copepods were observed. Histology was found to be useful and affordable technique in monitoring the overall health of mussel, providing useful pathologic information of the cells and tissues.

• Proceedings of the Korean Nuclear Society Conference
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• 1998.05b
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• pp.697-702
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• 1998

방사선에 의해 유발되는 난소내 난포의 폐쇄가 apoptosis를 매개로 하여 일어나는지 조사하고자 면역조직화학적 방법을 이용하여 실험을 수행하였다. 미성숙 생쥐 (3주, ICR)에 감마선을 조사하였으며, 조직절편을 제작한 후 TUNEL 방법에 의한 in situ 3'end labelling 면역조직화학 염색을 실시하였다. 전반적인 난소의 상태를 파악하고자, 일반적인 hematoxylin-eosin(HE) 염색을 실시하여 대조하였다. 면역조직화학 염색은 apoptosis가 일어난 난포를 시각적으로 구분해낼 수 있는 효과적인 방법임이 실험적으로 확인되었다. ApopTeg/HE 비율을 볼 때, R군은 6h에서 12h 에 높은 값을 보였고, 8d군에서 다시 증가하는 양상을 보였으며 이같은 결과는 실험동물의 난소내 과립세포의 apoptosis가 방사선 조사후 6시간부터 일어나기 시작하여 점차 증가된다는 사실을 나타낸 것이다. 결론적으로 방사선에 의한 난포의 폐쇄는 과립세포의 apoptosis를 매개로 하여 일어남을 알았다.