• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.21-21
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• 2003

경골어류의 뇌하수체에서는 두 종류의 생식선자극호르몬 (FSH, LH)이 생산되며, 이 호르몬들은 공통적인 α 쇄와 특이적인 β 쇄를 가진다. 연어과 어종들에서, FSH는 난황형성과 정자형성의 역할을 하며, LH는 배우자의 최종성숙을 조절한다. 냉수성 고유어종인 열목어 (Brachymystax lenok)의 멸종을 방지하고 종묘생산을 원활히 하기 위하여 먼저 열목어의 GTHα, FSHβ 및 LHβ 쇄를 cloning하여 염기서열을 결정하였다. 열목어 GTHα, FSHβ 및 LHβ의 cDNA는 산천어의 해당 cDNA와 높은 상동성 (각각 84, 95, 98%)을 보였다. 다음으로 기능적인 생식선자극호르몬을 제작하기 위해서 2개의 쇄를 single-strand로 연결하여 진핵세포를 숙주로하는 시스템에서 생식선자극호르몬을 생산할 수 있는 구조체인 FSHβ-GTHα (235 amino acids) 와 LHβ-GTHα (240 amino acids)를 각각 재조합하였다. 또한 각각의 융합단백질 생산용 구조체의 3'-말단에는 단백질추출이 용이하도록 histidine×6 구조를 첨가하였다. 이상의 단일쇄 FSH와 LH 유전자재조합 산물을 포유동물 유래의 세포 (CHO-K1)에 liposome chemical을 사용하여 유전자도입 후 세포에서 분비되는 단백질을 모니터링하였다. 배지를 부분정제한 후 SDS-PACE로 조사한 결과, LH 재조합 유전자를 도입한 후 48-60 시간째에 약 25 kDa의 단백질로서 관찰되었다. 현재 FSH 재조합 유전자에 대해서도 조사중이며, 향후 이를 재료로 하여 기능형 생식선자극호르몬을 생산하고 추출하기 위한 연구가 계속적으로 수행 될 것이다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.521-524
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• 2009

Response to nerve stimulation platform for implementing measures to detect finger movement has been functioning as an important factor. This stimulated finger on the nerve and muscle responses would vary. In other words, the finger movement of the muscle response to nerve stimulation and sensing Actuator for the H/W development is needed. In addition, a low power embedded CPU based on the top was used. H/W configuration portion of the isolation power, constant current control, High impedance INA, amplifier parts, and the stimulus mode and the Micro-control the status of current, AD converter Low Data obtained through the processing system is implemented.

• The Transactions of the Korean Institute of Power Electronics
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• v.6 no.4
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• pp.325-332
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• 2001

The vestibular system located in the inner ear controls reflex body posture and movement, It has the semicircular canals sensing an angular acceleration and the otolith organs sensing a linear acceleration. With this organic signal, medical doctor decide if a person has disease or not. To obtain this data, a precision stimular system is considered. Robust control is needed to obtain eye signals induced by off-vertical axis rotation because of an unbalanced load produced by tilting the axis of the system upto 30 degrees. In this study, off-vertical axis rotatory system with visual stimulation system are developed. This system is consisted of head mounted display for generating horizontal, vertical, and three dimensional stimulus patterns. Furthermore wireless recording system using RF modem is considered for noiseless data transmission. Detailed data was described.

• Korean Journal of Acupuncture
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• v.24 no.1
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• pp.13-25
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• 2007

목 적 : 흡연에 따른 건강적 피해가 많이 알려져 있음에도 불구하고 특히 여성 및 청소년 계층의 흡연이 증가하고 있으며 폐암 사망자수는 급격히 늘어나고 있는 상황이다. 완전한 금연이 실패하는 주요 이유는 담배의 중독성에서 비롯되며 금단현상을 극복하지 못해 일어나는 것으로 알려져 있다. 흡연의 중독성은 담배 주요성분 중의 하나인 니코틴(nicotine)에 의해 유발되며 따라서 모든 금연 요법 및 금연 치료보조제 들은 이 니코틴 작용을 어떻게 효과적으로 억제 또는 대체하느냐에 초점을 맞추고 있다. 최근 금연치료 요법으로 한방 침이 주목 받고 있으며 본 논문을 통해 한방 침의 금연효과에 대한 신경학적 기전을 고찰하고자 한다. 방 법 : 금연을 돕는 전형적인 보조 치료제 및 치료법이 몇 종류 개발되어 사용 중에 있으며 대표적으로 니코틴 대체 요법(nicotine replacement therapy, NRT)이나 항우울제로 사용되는 bupropion 등을 들 수 있다. 이 치료 방법들은 뇌의 도파민계 신경전달 체계에 영향을 미쳐 금연 효과를 발휘하게 되는데 실질적인 금연성공확률은 그리 높지 않은 것으로 알려져 있다 따라서 침의 자극에 대한 도파민계 신경전달 조절효과를 중심으로 고찰함으로써 침의 금연효과에 대한 의과학적 기전을 설명하고자 하였다. 결 과 : 침자극은 우수한 금연효과를 가져올 수 있는 치료법으로 기존의 금연 치료요법 및 치료제 들을 보완할 수 있는 보다 확실한 치료요법 중의 하나이며 특히 금연 후에 오는 금단현상을 효과적으로 완화시키는 작용을 한다. 그리고 이 같은 효과는 부분적으로 도파민계를 비롯한 신경전달계를 조절함으로써 가능한 것으로 판단된다. 결 론 : 본 논문을 통해 니코틴에 의한 금단현상의 신경학적 기전과 금연과 관련된 신경전달체계에 대한 침자극의 효능에 대해 고찰하였으며 기존의 금연보조치료법을 대체할 수 있는 우수한 의학적 치료법으로써의 침치료법을 제시하였다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.2
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• pp.204-210
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• 1999

The present studies were conducted to evaluate the effects of activin-A on gonadotropins (GTHs) release in testosterone-treated immature rainbow trout Oncorhpynchus mykiss. The administration of testosterone elevated pituitary level of GTH II but not of GTH I. In this study using primary cultures of dispersed pituitary cells in static incubation, dose-dependent increases in GTH II release was observed in the activin-treated group at day 3 of incubation (long-term incubation), but not at day 1 of incubation (short-term incubation). Dopamine, a potent inhibitor of gonadotropin-releasing hormone (GnRH)-stimulated GTH II release in rainbow trout, was only partially effective in decreasing actvin-induced GTH II release. Furthermore, salmon GnRH (sGnRH)-stimulated GTH II release was not potentiated by the pretreatment with activin. However, the control mechanisms of GTH I release by activin and other hormones were not observed in the all tested experiments. The results of these studies support the contention that in contrast with the usual stimulatory effects of activin on GTH release in mammals, activin exerts long-term stimulatory actions on GTH II release in rainbow trout. The control mechanism of GTH I release, however, is a question that remains to be elucidated.

• The Journal of the Korea Contents Association
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• v.12 no.4
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• pp.390-400
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• 2012

The satisfaction of customers, who are in a servicescape, will be subject to many environmental stimulations. In particularly, the servicescapes of the store and the customer characteristics are important factors that affecting the emotional commitment of consumers. In this study, the servicescape that consumers evaluated can be divided into physical and social factors. Furthermore, how these factors can affect the emotional commitment through perceived service quality and social interaction will be studied as well. In addition, the moderating effects of the individual characteristics at the perception of servicescape, such as optimum stimulation level and social appropriateness will be studied together. The results show that the physical factor of servicescape is one of the most important elements that affect "emotional commitment" and under this process; the perceived service quality is performed as a parameter. Besides, the social factor of servicescape, such as the other customer and companion affect can also affect emotional commitment through social interaction. On the other hand, through the analysis of optimum stimulation level and social appropriateness, we found that the higher the social appropriateness, the greater the effect of servicescape. Overall, in order to enhance the customer's emotional commitment, we must build high quality servicescape, meanwhile; the management of other customers and the companion are needed at the same time.

• Journal of Life Science
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• v.29 no.8
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• pp.922-940
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• 2019

Obesity, represented by abnormal fat accumulation due to an imbalance between energy intake and expenditure, is a major public health issue worldwide, leading to multiple noncommunicable diseases, including atherosclerosis, hypertension, type 2 diabetes, and cancer. Diverse solutions have been proposed to combat obesity. Attention has focused on two types of adipose tissues as a promising therapeutic target in obesity: traditional brown and beige or brite. Unlike energy-storing white adipose (endocrine) tissue, traditional brown adipose tissue and beige adipose tissue have energy-dissipating thermogenic properties. Both types of tissue are present in adult humans and inducible through external stimuli, such as cold exposure, ${\beta}3$-adrenergic receptor agonists, and phytochemicals. Among these stimuli, microbiota present in the human intestinal tract participate in multiple metabolic activities. Modulation of gut microbiota may offer a potent and possibly curative strategy against various metabolic diseases. Numerous studies have focused on the effects of established antiobesity treatments on the gut microenvironment or brown-adipose-tissue activation. In this review, we focus mainly on stimuli known to alleviate obesity, weight gain, and metabolic diseases, in addition to known and possible inter-relations between gut microbiota modulation and similar interventions and adipose tissue browning. The findings may pave the way toward new strategies against obesity.

• Korean Journal of Cognitive Science
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• v.21 no.1
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• pp.177-197
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• 2010

Three experiments compared two hypotheses on visual selection; perceptual load hypothesis and dilution hypothesis. The perceptual load hypothesis predicts that perceptual load of task-relevant processing determines the level of task-irrelevant processing whereas the dilution hypothesis predicts that competition for limited-capacity attentional resource, not perceptual load, determines the locus of selection. To compare the two hypotheses, we investigated the influence of perceptual load in visual search on response interference by a distractor. Experiment 1 and 2 manipulated perceptual load by the set size of a search array and the colors of a target and non-targets, respectively. As a result, distractor interference decreased with a set size regardless of perceptual load. In order to further test the set size effect, Experiment 3 manipulated the perceptual categories of non-targets and a distractor. The results showed that distractor interference decreased only when non-targets belonged to the same category as a distractor. Overall, the current findings support the dilution hypothesis, but not the perceptual load hypothesis, and provide the evidence that visual selection is constrained by capacity-limited, category-specific attentional resources.

• Journal of Periodontal and Implant Science
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• v.37 no.sup2
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• pp.371-384
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• 2007

연구 목적 : 면역 반응에서 B-1 세포의 정확한 역할은 아직 명확히 규명되지 않았으나 최근 B-1 세포가 면역의 내성을 야기하고 유지하는데 필요한 특성들을 가지고 있음이 밝혀지고 있다. 이에 본 연구에서는 B-1 세포 유래 단클론 항체의 특성과 항원 자극에 대한 B 세포의 동시자극자 (MHC Class, B7-2, 7-2) 발현을 평가하여 B 세포의 면역조절 기능을 알아보고자 한다. 연구 대상 및 방법 : B-1 세포 유래 단클론 항체를 형성하는 잡종세포주를 이용하여 다양한 내, 외인성 항원에 대한 단클론 항체의 반응 양상을 평가하였다. 여러 내, 외인성 항원으로 면역한 쥐의 복강과 비장 B 세포의 동시자극자의 발현을 Fluorescence Activated Cell Sorting (FACS)을 이용하여 평가하였다. 결과 : 최종적으로 단클론을 형성하는 2개의 클론을 형성하였고, 이 B-1 세포 유래 단클론 항체는 dose-saturable pattern을 띄는 다중 반응성을 나타내었다. FACS를 이용한 동시자극자의 발현 검사에서는 MHC 발현은 복강과 비장의 B 세포가 유사하였으나, B7-1과 7-2는 복강의 B 세포에서 더 뚜렷한 발현을 보여주었다. 결론 : B-1 세포 유래 단클론 항체는 다양한 내, 외인성 항원 자극에 대해 dose-saturable한 다중반응성을 나타낸다. 복강과 비장의 B세포는 내, 외인성 항원의 면역에 있어서 동시자극자 발현이 명확히 다른 양상을 나타냄을 확인할 수 있었다.

• Journal of Korean Ophthalmic Optics Society
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• v.19 no.3
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• pp.345-351
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• 2014

Purpose: The accommodative response and the near horizontal phoria were examined with additional spherical power to analyze the stimulus and response AC/A ratios that suggest reference data for the binocular vision. Methods: The open-field autorefractometer (Nvision-K 5001, Shin nippon) and modified thorington method (MIM card; Muscle Imbalance Measure card, Bernell) at 40 cm were utilized to measure the accommodative response and the near horizontal phoria for 81 persons ($20.89{\pm}1.92$ years old) with additional spherical power. The stimulus and the response AC/A ratios were calculated by gradient AC/A method. Results: The exophoria group showed the highest accommodative response ($1.92{\pm}0.26D$) at 40 cm, followed by orthophoria group and esophoria group($1.72{\pm}0.26D$ and $1.62{\pm}0.42D$, respectively) Meanwhile, the esophoria group showed the biggest ocular deviation for the near ($23.24{\Delta}$) followed by the orthophoria group and exophoria group ($19.76{\Delta}$ and $15.14{\Delta}$, respectively). The biggest difference of the stimulus and the response AC/A ratios was $1.72{\Delta}$ for the exophoria group with -2.00 D, while the one was $3.43{\Delta}$ for the esophoria group with +1.00 D. There was a significant difference between AC/A ratios for the exophoria group with -2.00 D, -1.00 D and the esophoria group with +3.00 D, +2.00D, +1.00D and -1.00D. Conclusions: The difference between stimulus and response AC/A was greater when increased minus spherical power for the exophoria group, while it was greater when increased plus spherical power for the esophoria group. Furthermore, the difference for the esophoria group was a greater than the one for the exophoria group.