• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.347-351
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• 1998

Plant regeneration via organogenesis from leaf and stipule explants of micropropagated shoots of strawberry (Fragaria $\times$ ananassa cv. Suhong) was achieved. Leaf and stipule explants were detached from shoot-tip cultured shoots and cultured on MS medium with various combinations of BA and NAA under light or dark condition. Shoot regeneration from leaf explant was observed after 3 weeks in culture and was good at the high ratio of BA and NAA among various combination treatments. The highest shoot regeneration frequency from leaf explants was obtained with 1.0 mg/L BA and 0.1 mg/L NAA, in which 31.1% shoot regeneration frequency(1.7 shoots per leaf explant) was yielded. In case of stipule explants, shoot regeneration was largely affected by plant growth regulators during incubation under dark condition for initial 4 weeks but not under continuous light condition. The combination treatment with 2.0 mg/L BA and 0.1 mg/L NAA showed the most excellent shoot regeneration from stipule explants, where 44.4% regeneration frequency(4.0 shoots per explants) obtained. Regenerated shoots were rooted on MS medium with 0.1 mg/L NAA after shoot elongation, and the plantlets regenerated were transferred to soil mixtures with vermiculite and perlite for acclimation.

• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.84-88
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• 2010

To establish the system of In vitro plant regeneration, the floral bud and leaf explants of Sedum rotundifolium were cultured on the MS media supplemented with different concentration of 2,4-D, NAA, and BA. The callus induction was more effective in the floral explants than the leaf explants, and was the best on MS medium containing 1.0 or 2.0 mg/L 2,4-D and 1.0 mg/L BA. The highest numbers of shoots were regenerated when callus were cultured on MS medium containing 2.0 mg/L 2,4-D and 1.0 mg/L BA for 8 weeks. The normal root formation from shoot was effective on the MS medium containing IAA alone. The regenerated plantlets were transferred to the pot and acclimatized successfully.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.3
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• pp.372-378
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• 2011

We extracted polysaccharide from the sea hare, Aplysia kurodai, purified it partially, and experimented its immune response using the human blood lymphocytes and macrophage cell lines. Aplysia kurodai polysaccharide fraction (APF) improved the growth of the T cell (Jurkat) up to 40% by treatment for 48 hours, and decreased the growth of blood cancer, Jiyoye cell line. The APF on RAW 264.7 cell also increased interleukin-12 up to 47%. In contrast, the secretion of interleukin-2 and interferon-gamma by treatment of only APF or APF and concanavalin A on Jurkat for 24 hours and 48 hours didn't influence significantly. These results suggest that the APF has possible immune regulating ability.

• Proceedings of the Korean Vacuum Society Conference
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• 2000.02a
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• pp.125-125
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• 2000

백금 스퍼터 증착시 아르곤에 산소와 같은 첨가 가스를 사용할 경우 산화막에 대한 접착력이 좋아지며 백금 박막의 우선배향성을 조절할 수 있음이 알려져 있다. 이러한 첨가 가스는 백금 박막에 상당량 포함되며 스퍼터링 후 열처리 과정에서 탈착되는 것으로 알려져 있다. 후열처리 도중 첨가 가스의 탈착 거동이 백금 박막의 미세구조, 조성 및 전기 전도도 등과 같은 제반 물성에 영향을 미칠 것이라 추정된다. 본 연구에서는 백금의 스퍼터링 시 질소를 첨가하여 질소가 포함된 백금 박막을 증착한 후 질소 탈착 거동을 연구하기 위해 실시간 타원해석기(in situ ellipsometer)를 이용하여 진공열처리(15mTorr)하면서 온도변화에 따른 유효굴절율(n)과 소광계수(k) 값을 구하였다. 또한 산소를 첨가하여 얻은 백금 박막의 결과와 비교하여 백금 박막내에 포함된 산소와 질소의 탈착 거동의 차이를 조사하였다. 산소를 이용하여 우선배향성이 (200)으로 조절된 박막의 경우 n과 k의 급격한 변화가 관찰되었으며 이로부터 55$0^{\circ}C$ 온도에서 산소가 급격히 빠져나감을 추측할 수 있었으며 열처리 후에는 백금 bulk 값에 가까운 값을 가짐을 알 수 있었다. 한편, 질소를 사용하여 (200)으로 우선배향성이 조절된 박막의 경우 n,k 값의 후열처리 도중의 변화 양상은 스퍼터링 압력에 크게 의존하는 것으로 나타났다. 22mTorr에서 스퍼터링한 박막의 경우 23$0^{\circ}C$ 부근에서 굴절률과 미세구조의 변화가 있음을 관찰할 수 있었으나, 10mTorr에서 스퍼터링한 시편의 경우 굴절률의 변화양상은 산소를 상요한 경우와 매우 유사한 거동을 나타내지만 열처리 후에는 상대적으로 낮은 n,k 값을 나타내고 있었다. 또한 열처리 시편의 미세구조 변화에 대한 분석 결과 산소 사용의 경우는 측정온도 범위내에서는 후 열처리 후에도 박막내에 hole이나 hillock 등이 관찰되지 않아 bulk 값에 가까운 n, k 값을 가지지만, 질소 사용의 경우는 hole, 표면 거칠기, 혹은 스퍼터링 중에 인입된 질소의 탈착이 완전히 이루어지지 못해 bulk 값과 다르게 나온 것으로 생각된다.

• Korean Journal of Plant Tissue Culture
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• v.27 no.3
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• pp.197-202
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• 2000

The effect of cultural media and growth regulators on multiple plant regeneration from leaf explants of Heloniopsis orientalis (Thunb.) Tanaka was evaluated. The highest percentage of shoot and root formation were 20 and 30% on MS medium treated at 3.0 mg $l^{-1}$ of zeatin, respectively. Also 67 and 33% of high shoot formation appeared on 1/2 MS and 5 culture medium treated at zeatin 1.0 and 3.0 mg $l^{-1}$ respectively. With MS treated at 0.5 mg $l^{-1}$ of 2,4-D 1/2 MS and B5 culture media treated at each 1.0 and 3.0 mg $l^{-1}$ of zeatin the highest ratios of plant produced were 100, 280 and 310 % respectively relative to the other treatments. Generally, there was highest possibility for multiple propagation of Heloniopsis orientalis (Thunb.) C. Tanaka with B5 culture media supplemented 3.0 mg $l^{-1}$ of zeatin.

• International Area Studies Review
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• v.14 no.3
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• pp.312-338
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• 2010

This paper investigates the value relevance of advertising activity over the period from 2001 to 2009 in the listed Korean stock markets(KOSPI and KOSDAQ). In addition, this paper divide all sample firm into several subsample, based on R&D intensity and export ratio in total sales. The empirical result of this paper shows following two evidence; First, advertising intensity, which proxies for advertising activity, is significantly related to firm value at the 1% level of significance. Second, advertising activity of high R&D intensity and high export ratio sample firms has more significant value relevance than for firms with low R&D intensity and low export ratio. The empirical results suggest that moderation effect of R&D intensity and export ratio are very important factor in determining the value relevance of advertising activity.

• Journal of Bio-Environment Control
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• v.13 no.4
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• pp.258-265
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• 2004

The experiment was conducted to investigate the effect of EC and pH of nutrient solution on the growth and quality of the single-stemmed rose grown in a rose factory installed with hydroponic system. The growth and quality of the single-stemmed rose were not significantly different from each other with the EC of the nutrient solutions $1.0\~3.5dS{\cdot}m^{-1}$, which resulted in concluding high concentration of the nutrient solution was not necessary. The optimum range of the EC for single-stemmed rose was $1.5\~2.0dS{\cdot}m^{-1}$ considering plant growth, photosynthetic and transpiration rates. The optimum range of the pH for good plant growth without any visible physiological disorder was $4.0\~6.0$. Therefore, to keep the pH of the nutrient solution for rose low compared to other plants was beneficial for plant growth and uptake of the mineral ions.

• Journal of Bio-Environment Control
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• v.16 no.3
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• pp.269-273
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• 2007

Freezing hardiness of winter bud and branch according to dormancy level and low temperature, in persimmon (Diospyros kaki) cultivars, was investigated by electrolyte leaching rate, triphenyltetrazolium chloride (TTC) test, and sprouting. Electrolyte leaching rate was lowest in branch of 20th January and was highest in the 20th March. The electrolyte leaching rate of 'Fuyu' and 'Cheongdobansi' was high in the 20th January and was low in the 20th February, but 'Uenishiwase' and 'Nishimurawase' was opposed to that. 'Hachiva' was the middle level in the cultivars. Absence rate by TTC test was highest in the 20th January and was low in the others. The 20th March had a great decrease in $-10^{\circ}C$ treatment. The absence rate of 'Fuyu' and 'Uenishiwase' was low in the 20th January and March and was high in the 20th February. 'Nishimurawase' and 'Hachiya' had a high level irrespective of dormancy level. Sprouting was highest in the 20th February and was lowest in 20th March. Most cultivars were not sprout in $-20^{\circ}C$ treatment and 'Fuyu', 'Nishimurawase' and 'Cheongdobansi' was a little high level irrespective of dormancy level. 'Hachiya' was only high in the 20th January. Thus, freezing hardiness of persimmon was very weak low temperature after dormancy breaking and was not different between astringent and non-astringent persimmon.

• Journal of Korean Society of Steel Construction
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• v.27 no.1
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• pp.31-42
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• 2015

The plastic stress distribution method and the strain compatibility method are the two representative methods to calculate the P-M interaction strength of RCFT (rectangular concrete filled tube) columns. The plastic stress distribution method is approximate while the stress compatibility method should approach the exact solution if accurate constitutive relations of the materials involved are used. Recent study by the authors pointed out that, because of lack of accurate constitutive model for the concrete confined by the rectangular steel tube, no strain compatibility method according to the current structural provisions provides a satisfactory prediction of the P-M interaction strength of RCFT columns under various material combinations. An empirical constitutive model which can capture the stress-strain characteristics of the confined concrete of RCFT columns is proposed based on analyzing extensive exisitng test database. The key idea was to define the concrete crushing strain as a function of steel-to-concrete strength ratio and width-to-thickness ratio of steel tube. It was shown that the proposed model leads to more accurate and more consistent prediction of the P-M interaction strength of RCFT columns under general design conditions.

• Journal of dental hygiene science
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• v.15 no.4
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• pp.413-418
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• 2015

Recent cross-sectional studies indicate that obesity is a risk factor for periodontal disease. This study was aimed to investigate whether the four-week weight control program including caloric restriction and exercise training could have an effect on periodontal health. Forty-one obese (body mass index [BMI] ${\geq}25.0$) and five overweight ($23.0{\leq}BMI<25.0$) students participated in the weight control program. Anthropometric data and oral examination data were collected at the baseline and at the 27th day. BMI, waist hip ratio (WHR), and percent of body fat (PBF) of the subjects decreased significantly, but gingival index, sites with bleeding on probing (BOP), and sites with shallow pocket depth didn't show the significant changes in paired t-test. There was no difference in the outcomes according to smoking, drinking alcohol, and sex. Nevertheless, PBF and sites with BOP (r=0.777) and WHR and sites with shallow pocket depth (r=0.444) showed positive correlations. PBF accounted for 58.9% of the variance in sites of BOP in regression analysis. We suggested that obesity might relate with periodontal health, although it was not clear whether weight control could influence on periodontal health directly.