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http://dx.doi.org/10.3746/jkfn.2011.40.3.372

Immune Regulating Effect of Polysaccharide Fraction from Sea Hare (Aplysia kurodai)  

Park, Si-Hyang (Dept. of Seafood Science and Technology/ Institute of Marine Industry, Gyeongsang National University)
Choung, Se-Young (Dept. of Hygienic Chemistry, College of Pharmacy, Kyung Hee University)
Choi, Yeung-Joon (Dept. of Seafood Science and Technology/ Institute of Marine Industry, Gyeongsang National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.40, no.3, 2011 , pp. 372-378 More about this Journal
Abstract
We extracted polysaccharide from the sea hare, Aplysia kurodai, purified it partially, and experimented its immune response using the human blood lymphocytes and macrophage cell lines. Aplysia kurodai polysaccharide fraction (APF) improved the growth of the T cell (Jurkat) up to 40% by treatment for 48 hours, and decreased the growth of blood cancer, Jiyoye cell line. The APF on RAW 264.7 cell also increased interleukin-12 up to 47%. In contrast, the secretion of interleukin-2 and interferon-gamma by treatment of only APF or APF and concanavalin A on Jurkat for 24 hours and 48 hours didn't influence significantly. These results suggest that the APF has possible immune regulating ability.
Keywords
sea hare; Aplysia kurodai; polysaccharide; immune regulating;
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1 Kim CH, Kwon MC, Kim HS, Bae GJ, Ahn JH, Choi GP,Choi YB, Ko JR, Lee HY. 2007. Enhancement of immune activities of Kadsura japonica Dunal. through conventional fermentation process. Korean J Medicinal Crop Sci 15:162-169.   과학기술학회마을
2 Wang YP, Cheng ML, Zhang BF, Mu M, Zhou MY, WuJ, Li CX. 2010. Effect of blueberry on hepatic and immunological functions in mice. Hepatobiliary Pancreat Disint 9:164-168.
3 Schupp PJ, Kohlert-Schupp C, Whitefield S, Engemann A,Rohde S, Hemscheidt T, Pezzuto JM, Kondratyuk TP, ParkEJ, Marler L, Rostama B, Wright AD. 2009. Cancer chemopreventive and anticancer evaluation of extracts and fractions from marine macro- and micro-organisms collected from twilight zone waters around guam. Nat Prod Commun4: 1717-1728.
4 Suh SH, Suh YS. 2007. Function of cytokines in immunological memory. http://bric.postech.ac.kr/myboard/read.php?Board=review0&id=1650. ISSN 1598-8767.
5 Chae SY, Kim MJ, Kim DS, Park JE, Jo SK, Yee ST. 2007.Effect of Asterina pectinifera extracts on the activation of immune cells. J Korean Soc Food Sci Nutr 36: 269-275.   과학기술학회마을   DOI   ScienceOn
6 Kwon MC, Han JG, Jeong HS, Qadir SA, Choi YB, Ko JR,Lee HY. 2008. Enhancement of immune activities of Opuntia ficus-indica L. var. Miller by ultrasonification extraction process. Korean J Medicinal Crop Sci 16: 1-8.
7 Pzaki L, Leonard WJ. 2002. Cytokine and cytokine receptor pleiotropy and redundancy. J Biol Chem 277: 29355-29358.   DOI
8 Kim DW, Cho SB, Yun CH, Jeong HY, Chung WT, ChoiCW, Lee HJ, Nam IS, Suh GH, Lee SS, Lee BS. 2007.Induction of cytokines and nitric oxide in murine macrophages stimulated with enzymatically digested Lactobacillus strains. J Microbiol 45: 373-378.
9 Smith KA, Lachman LB, Oppenheim JJ, Favata MF. 1980.The functional relationship of the interleukins. J Exp Med 151: 1551-1556.   DOI
10 Wang SY, Hsu ML, Hsu HC, Tzeng CH, Lee SS, ShianoMS, Ho CK. 1997. The anti-tumor effect of Ganoderma lucidum is mediated by cytokine released from activated macrophages and T lymphocytes. Intl J Cancer 70: 699-705.   DOI
11 Kim GY, Lee JY, Lee JO, Ryu CH, Choi BT, Jeong YK,Lee KW, Jeong SC, Choi YH. 2006. Partial characterization and immunostimulatory effect of a novel polysaccharideprotein complex extracted from Phellinuslinteus. Biosci Biotechnol Biochem 70: 1218-1226.   DOI
12 Schuchter LM, Esa AH, May WS, Laulis MK, Pettit GR,Hess AD. 1991. Successful treatment of murine melanoma with bryostatin 1. Cancer Res 51: 682-687.
13 Kim DH, Park JH, Kim JH, Kim CH, You JH, Kwon MC,Lee YY. 2005. Enhancement of immune activities of Ephedrae herba and Rubi fructus at low temperature extraction.Korean J Medicinal Crop Sci 13: 81-86.   과학기술학회마을
14 Kim CH, Seo HJ, Hwang EY, Kim EJ, Go HJ, Kim IH, SeoJK, Moon JM, Huh MD, Park NG. 2001. Purification of myomodulin A and myomodulin E from the central nervous system of the sea hare, Aplysia kurodai. J Korean Soc Fish34: 279-284.   과학기술학회마을
15 Antonov I, Kandel ER, Hawkins RD. 2010. Presynaptic and postsynaptic mechanisms of synaptic plasticity and metaplasticity during intermediate-term memory formation in Aplysia. J Neurosci 30: 5781-5791.   DOI
16 Specic K, Kauferstein S, Mebs D, Turk T. 2010. Biological activities of aqueous and organic extracts from tropical marine sponges. Mar Drugs 8: 1550-1566.   DOI
17 Wu D, Meydani SN, Meydant M, Hayek MG, Huth P,Nicolsi RJ. 1996. Immunologic effects of marine- and plantderived n-3 polyunsaturated fatty acids in nonhuman primates. Am J Clin Nutr 63: 273-280.   DOI
18 Mohammad RM, Varterasian ML, Almatchy VP, HannoudiGN, Pettit GR, Al-Katib A. 1998. Successful treatment of human chronic lymphocytic leukemia xenografts with combination biological agents auristatin PE and bryostatin 1. Clin Cancer Res 4: 1337-1343.
19 Yoon BY, Choi BD, Choi YJ. 2010. Extraction of glycosaminoglycan from sea hare, Aplysia kurodai, and its functional properties 1. optimum extraction of polysaccharide and purification of glycosaminoglycan. J Korean Soc FoodSci Nutr 39: 1640-1646.   과학기술학회마을   DOI
20 Yoon BY, Choi BD, Bae DW, Choi YJ. 2010. Extraction of glycosaminoglycan from sea hare, Aplysia kurodai and its functional properties 2. structural properties of purified glycosaminoglycan. J Korean Soc Food Sci Nutr 39: 1647-1653.   과학기술학회마을   DOI
21 Kwon OK, Min DK, Lee JR, Lee JS, Je JG, Choe BL. 2001.Korean mollusks with color illustration. Hangul press,Busan, Korea.
22 Kim WS. 2008. The study on anticancer activity from sea hare (Aplysia kurodai) eggs. PhD Dissertation. Jeju National University, Jeju, Korea.
23 Choi YJ, Han SY. 1985. Protein and amino acid compositionsin echiurid and sea hare muscles. Korean Soc Fisheries Aquatic Sci 18: 550-556.   과학기술학회마을
24 Shin MO. 2010. The antioxidative and antimicrobial effects of internal organs of Aplysia kurodai fractions. J KoreanSoc Food Sci Nutr 39: 1433-1438.   과학기술학회마을   DOI