Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of plant growth regulators on plant regeneration from the Sedum rotundifolium D. Lee

둥근잎꿩의비름(Sedum rotundifolium D. Lee)의 식물체 재분화에 미치는 식물생장조절제의 영향

  • Kwon, Hye-Kyoung (Department of Biology, Colleges of nature sciences, Kongju National University) ;
  • Yoon, Eui-Soo (Department of Biology, Colleges of nature sciences, Kongju National University)
  • 권혜경 (공주대학교 자연과학대학 생명과학과) ;
  • 윤의수 (공주대학교 자연과학대학 생명과학과)
  • Received : 2010.03.12
  • Accepted : 2010.03.26
  • Published : 2010.03.31
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Abstract

To establish the system of In vitro plant regeneration, the floral bud and leaf explants of Sedum rotundifolium were cultured on the MS media supplemented with different concentration of 2,4-D, NAA, and BA. The callus induction was more effective in the floral explants than the leaf explants, and was the best on MS medium containing 1.0 or 2.0 mg/L 2,4-D and 1.0 mg/L BA. The highest numbers of shoots were regenerated when callus were cultured on MS medium containing 2.0 mg/L 2,4-D and 1.0 mg/L BA for 8 weeks. The normal root formation from shoot was effective on the MS medium containing IAA alone. The regenerated plantlets were transferred to the pot and acclimatized successfully.

둥근잎꿩의비름 (Sedum rotundifolium D. Lee)의 기내 식물체 재분화 체계를 확립하기 위하여, 생장조절물질을 다르게 조합한 MS 배지에 화뢰와 잎 절편체를 치상하여 캘러스 유도와 식물체 재분화에 미치는 영향을 조사하였다. 캘러스 유기는 화뢰절편체에서 보다 잎절편체에서 왕성하게 관찰되었으며, 1.0 ~ 2.0 mg/L NAA와 1.0 mg/L BA의 혼합첨가 배지에서는 캘러스의 형성률이 100%로 가장 높았으며, 캘러스의 증식도 매우 왕성하였다. 또한, 2.0 mg/L 2,4-D에 1.0 mg/L BA를 혼합처리 한 배지에서 유도된 캘러스를 치상하여 8주간 배양하였을 때 가장 많은 신초가 유도되었고, IAA를 단독처리 한 MS 배지에서 보다 더 정상적이며 성장이 양호한 뿌리의 분화가 관찰되었다. 재분화 식물체는 식물생장조절물질이 첨가 되지 않은 MS배지로 계대배양하여 생장시킨 후 인공토양의 화분으로 옮겨 관찰한 결과 100%의 생존률을 보이며 정상적으로 생육하여 재분화 식물체를 대량으로 획득하였다.

Keywords

References

  1. Ahn JH, Lee SY (2004) Effects of growth regulators on callus induction and plant regeneration from leaf explants of Sedum sarmentosum. Kor J Plant Biotechnol 31:25-29 https://doi.org/10.5010/JPB.2004.31.1.025
  2. Bae CH, Tohyama K, Lee SC, Lim YP, Kim HI, Song PS, Lee HY (2001) Efficient plant regeneration using mature seed-derived callus in Zoysiagrass (Zoysia japonica Steud.). Kor J Plant Tissue Cult 28:61-67
  3. Chae YA, Park SU (1993) Callus induction and somatic embryogenesis in suspension culture of Rehmannia glutinosa. Kor J Med Crop Sci 1:184-190
  4. Chen TH, Lam L, Chen SC (1985) Somatic embryogenesis and plant regeneration from cultures inflorescence of Oryza sativa L.. Plant Cell Tissue Organ Cult 21:111-117
  5. Gulati A, Jaiwal PK (1992) In vitro induction of multiple shoots and plant regeneration from shoot tips of mung bean (Vigna radiata L. Wilczek). Plant Cell Tissue Organ Cult 29:199-205 https://doi.org/10.1007/BF00034353
  6. Jeong JH (1999) Growth and flowering response of potted Sedum rotundifolium to low temperature, photoperiod and $GA_3$. J Kor Soc Hort Sci 40: 761-764
  7. Jeong JH (2001) Critical daylength for flower bud formation and development of Sedum rotundifolium. J Basic and Life Res Sci 1:219-222
  8. Jeong JH , Kwon ST (2003) Induction daylength period for flower bud formation and development of Sedum rotundifolium. J Kor Flower Res Soc 11:225-228
  9. Kang MS, Choi JD, Kim KW (1998) Effects of culture conditions on adventitious bud formation from callus of Gladiolus ‘Topaz’. J Kor Soc Hort Sci 39:338-342
  10. Kim HY, Cho HJ, Kim EY, Kim MY, Park HB (2007) Mass propagation by in vitro culture of Bupleurum latissimum Nakai. Kor J Plant Res 20:367-374
  11. Kim YH (2001) Effects of BA, NAA, 2,4-D and AgNO3 treatments on the callus induction and shoot regeneration from hypocotyls and cotyledon of Sesame (Sesamum indicum L.). J Kor Soc Hort Sci 42:70-74
  12. Koroch A, Juliani HR, Kapteyn J, Simon JE (2002) In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell Tissue Organ Cult 69:79-83 https://doi.org/10.1023/A:1015042032091
  13. Kwon ST, Jeong JH (1999) Genetic relationship among Sedum species based on morphological characteristics and RAPD analysis. Kor J Hort Sci Technol 17:490-493
  14. Moon HK, Suk GY, Kim SC (1997) Micropropagation of a rare species, Forsythia saxatillis N. through tissue culture. J Kor For Soc 86:430-434
  15. Moon HK, Suk GY, Kwon YJ, Son SH (1999) Micropropagation of a rare species, Abeliophyllum disticchum Nakai. via axillary bud culture. Kor J Plant Tissue Cult 26:133-136
  16. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:487-497
  17. Park CH, Yu CY, Kim DW, Cho HK Park KS, Seo JS, Ahn SD, Chang BH (1994) Plant regeneration of Bupleurum spp. through somatic tissue culture. Kor J Med Crop Sci 2:60-66
  18. Ryu JH, Doo HS, Kwon TH (1992) Induction of haploid plants by anther culture in sesame (Sesamum indicum L.). Kor J Plant Tissue Cult 19:171-177
  19. Yoon ES (1997) Effect of plant growth regulators on plant regeneration from lesf and stem explants cultures of Sedum erythrostichum Miq. Kor J plant Tissue Cult 24:285-289

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