• Journal of Applied Biological Chemistry
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• v.64 no.4
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• pp.375-382
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• 2021

Biorenovation is a microbial enzyme-based structural modification of component compounds in natural products and synthetic compounds including plant extracts with the potential benefits of improved biological activities compared with its reaction substrates. In this study, we investigated the anti-inflammatory activity of Distylium racemosum leaf extract and D. racemosum leaf biorenovation extract (DLB). As a result, DLB inhibited nitric oxide, prostaglandin E2, and inflammatory cytokines including tumor necrosis factor-α, interleukin-6, interleukin-1β at non-toxic concentrations. In addition, DLB significantly inhibited inducible nitric oxide synthase and cyclooxygenase-2 on LPS-treated RAW 264.7 macrophages. Based on these results, we suggest that the DLB could be used as a potent anti-inflammatory agents. It also suggests that the application of biological evolution has potential usefulness to increase the practical value of natural products.

• Journal of Life Science
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• v.30 no.3
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• pp.221-229
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• 2020

Distylium racemosum is an evergreen tree growing wild on Jeju Island, which has been reported to exert biological activity. Obesity is induced by genetic, metabolic, environmental, and other factors. Among these, certain bacterial and viral infections have been shown to cause obesity, which is known as infectobesity. Human adenovirus (HAdV)-36 is one of the viruses that are known to cause infectobesity in humans. Unlike extensive research on preventing obesity and developing anti-obesity drugs, little research has been conducted specifically on the prevention and treatment of infectobesity. Therefore, this study aimed to evaluate the effects of phytochemicals from D. racemosum on the replication of HAdV-36. A549 cells infected with HAdV-36 were treated with an ethyl acetate fraction of a D. racemosum leaf extract (DRE), and the virus titer was calculated based on the hemagglutination (HA) titer. The results showed a concentration-dependent inhibitory effect of DRE treatment on HA titers. DRE treatment was also found to inhibit the cytopathic effects of the virus and the expression of viral genes. Quercitrin was identified as the constituent of DRE exerting an inhibitory effect on HAdV-36 replication. This study shows that DRE can be used as a candidate substance for the development of treatment for HAdV-36 infections. In addition, this study provides a basis to further investigate DRE for the development of anti-infectobesity medication.

• Korean Journal of Clinical Laboratory Science
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• v.48 no.2
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• pp.62-67
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• 2016

Anti-oxidant activity of 600 medicinal plants from Jeju was analyzed. Extracts from the leaves of Distylium racemosum have the highest anti-oxidant activity. D. racemosum is an oriental medicinal plant belonging to the Hamamelidacea and grows in the wild in Jeju. This study was conducted to evaluate the antioxidant and cell viability of different fractions (n-hexane, methylene chloride, ethyl acetate, buthanol, DW) from D. racemosum. Anti-oxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity test and total phenolic content. Cell viability was determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) cell viability assay on HepG2 and A549 cells. Among various extracts, the ethyl acetate fraction showed the highest DPPH radical scavenging activity, reaching approximately 93% at 0.5 mg/mL, higher than that of quercetin used as a positive control. Ethyl acetate fractions showed the highest total phenolic content at 505 mg GAE/g. The phenolic content of each extract showed association with DPPH radical scavenging activity. The ethyl acetate extracts were resistant against hydrogen peroxide ($H_2O_2$) treatment in the MTT cell viability assay and showed a higher cell protective effect than other fraction extracts. These results suggest that the ethyl acetate fraction might be a source of anti-oxidants of D. racemosum.

• Journal of Bio-Environment Control
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• v.21 no.1
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• pp.66-73
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• 2012

This study was conducted to develope new indoor plants and to investigate the effects of light intensity on the growth of $Distylium$ $racemosum$, $Osmanthus$ $heterophyllus$ and $Damnacanthus$ $indicus$ under 100 lux, 1,000 lux and 2,500 lux fluorescent lighting for six months in the environment-controlled growth chambers. 1. $Distylium$ $racemosum$ : Most of $Distylium$ $racemosum$ under 100 lux light intensity were blighted in two months, whereas it was 100% of survival under 1,000 lux and 2,500 lux after six months. Plant height, number of leaf, leaf width and leaf length became higher as light intensity increased. The plants maintained under 2,500 lux showed the greatest plant height and leaf number. 2. $Osmanthus$ $heterophyllus$ : $Osmanthus$ $heterophyllus$ under 100 lux light intensity were blighted in two months, whereas it was 100% of survival under 1,000 lux and 2,500 lux after six months. However, under 1,000 lux, it paused plant height and was not increased in leaf number any more. A plant growth status showed the highest value under 2,500 lux in all conditions. 3. $Damnacanthus$ $indicus$ : $Damnacanthus$ $indicus$ was defoliated and blighted under 100 lux light intensity in two months, whereas it was grown properly with 1,000 lux or above. However, the growth under 2,500 lux of $Damnacanthus$ $indicus$ was superior to other treatments. But, $Damnacanthus$ $indicus$ under 1,000 lux after 6 months was more favorable chlorophyll contents, leaf length and leaf width than 2,500 lux. As increasing slightly of chlorophyll contents and leaf growth under 1,000 lux, $Damnacanthus$ $indicus$ could be utilized highly to the indoor ornamental plant.

• Korean Journal of Pharmacognosy
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• v.35 no.4 s.139
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• pp.271-275
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• 2004

Distylium racemosum Sieb. Et Zucc contains some compounds inhibit -amylase activity in experimental conditions. The inhibitory test showed that 50% acetone extracts from the bark and leaves of the plant strongly inhibited salivary -amylase activity. Proanthocyanidin(PA) which has strong inhibitory activity was extracted from the leaves by chromatography on Sephadex LH-20. The inhibitory activities and the inhibition kinetics of the PA were studied against three kinds of enzymes: human salivary ${\alpha}-Amylase$ (SAA), pork pancreatin ${\alpha}-Amylase$ (PAA) and yeast ${\alpha}-Glucosidase$ (AG). Then the activities of PA against SAA, PAA and AG were compared with those of acarbose, a commercial agent. The inhibitory activities of PA were stronger than those of acarbose. Inhibition kinetics of the PA showed competitive inhibition for SAA and PAA, and non competitive inhibition for GA.

• Korean Journal of Environment and Ecology
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• v.21 no.2
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• pp.141-148
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• 2007

To investigate the forest community structure ranging from 200 meters to 1,350 meters above sea level at Donnaeko valley of Mt. Hallasan in 2006, 15 plots were surveyed. According to the classification analysis by TWINSPAN, the plant communities were divided into five groups of Castanopsis sieboidii community, Castanopsis sieboldii - Quercus acuta community, mixed forest, Carpinus laxiflora - Quercus serrata community and deciduous broad-leaved forest. 22 species of evergreen broad-leaved trees such as Castanopsis sieboldii, Quercus acuta, Distylium racemosum, Camellia japonica, Eurya japonica, Ligustrum lucidum, Ilex crenata, Daphnipyllum macropodum, etc. were growing at Donnaeko valley. According to the attitudinal distribution of evergreen broad-leaved trees, Castanopsis sieboidii was a dominant species distributed from 200 meters to 350 meters above sea level, Castanopsis sieboldii and Quercus acuta were dominant species distributed from 400 meters to 600 meters above sea level and Quercus acuta was a dominant species distributed from 660 meters to 700 meters above sea level. Ilex crenata, Daphniphyllum macropodum, Elaeagnus glabra were distributed up to 1,350 meters above sea level in Donnaeko.

• Korean Journal of Plant Resources
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• v.22 no.1
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• pp.71-77
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• 2009

Antioxidative activity and ${\alpha}$-amylase inhibitory activity were investigated in the methanol extracts of 289 species of angiosperm plants native to Jeju Island in order to select the plant species containing bioactive materials for functional foods or medicines. The antioxidative activity, estimated by the DPPH radical scavenging capacity, was high in the galla of Rhus chinensis, the fruit of Platycarya strobilacea, and the stem of Cedrela sinensis. Particularly, the antioxidative activity of Rhus chinensis galla (94.2${\pm}$0.8%) was very high even though compared to those of BHA (61.7${\pm}$0.6%) and ascorbic acid (53.6${\pm}$0.7%). The ${\alpha}$-amylase inhibitory activity was 80% or above in 9 species including Cornus macrophylla (stem, leaf), Distylium racemosum (leaf) and Vaccinium oldhami (leaf). These results suggest that these plants could be potentially utilizable to develop bioactive materials for functional foods or medicines.

• Journal of agriculture & life science
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• v.46 no.3
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• pp.27-36
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• 2012

The purpose of this study was to investigate the structure of the vegetation on the trail of Hallyeo Marine & Coastal National Park. Twenty six $10{\times}10m$ plots were established along the Jisimdo trail. The vascular plants were surveyed from March 2011 to October 2011. Camellia japonica was a major woody species in the study area, while Pinus thunbergii and Zanthoxylum ailanthoides were co-dominant tree species. There were positive correlations between Distylium racemosum and Ligustrum japonicum var. japonicum; Callicarpa japonica var. luxurians and Eurya japonical; Actinodaphne lancifolia and Cinnamomum japonicum; A. lancifolia and Machilus japonica. Positive correlations were found between A. lancifolia and P. thunbergii; Z. ailanthoides and C. japonicum. Species diversity (H') was ranged from 0.51907 to 1.0217, and was relatively low compared to those of same national parks. 10 populations of Milletia japonica which are rare and endemic species, were recorded from the list of rare and endemic species of the Ministry of Environment near by ammo dumps, this study recommend the long term habitat monitoring of the species.