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Inhibitory Effects of Proanthocyanidin Extracted from Distylium racemosum on ${\alpha}-Amylase$ and ${\alpha}-Glucosidase$ Activities  

Ahn, Jin-Kwon (Div. Biotechnology, Korea Forest Research Institute)
Park, Young-Ki (Div. Biotechnology, Korea Forest Research Institute)
Park, So-Young (Div. Biotechnology, Korea Forest Research Institute)
Kim, Yong-Mu (Division of Biotechnology, Kangwon National University)
Rhee, Hae-Ik (Division of Biotechnology, Kangwon National University)
Lee, Wi-Young (Div. Biotechnology, Korea Forest Research Institute)
Publication Information
Korean Journal of Pharmacognosy / v.35, no.4, 2004 , pp. 271-275 More about this Journal
Abstract
Distylium racemosum Sieb. Et Zucc contains some compounds inhibit -amylase activity in experimental conditions. The inhibitory test showed that 50% acetone extracts from the bark and leaves of the plant strongly inhibited salivary -amylase activity. Proanthocyanidin(PA) which has strong inhibitory activity was extracted from the leaves by chromatography on Sephadex LH-20. The inhibitory activities and the inhibition kinetics of the PA were studied against three kinds of enzymes: human salivary ${\alpha}-Amylase$ (SAA), pork pancreatin ${\alpha}-Amylase$ (PAA) and yeast ${\alpha}-Glucosidase$ (AG). Then the activities of PA against SAA, PAA and AG were compared with those of acarbose, a commercial agent. The inhibitory activities of PA were stronger than those of acarbose. Inhibition kinetics of the PA showed competitive inhibition for SAA and PAA, and non competitive inhibition for GA.
Keywords
Distylium racemosum; proanthocyanidin; ${\alpha}-Amylase$; ${\alpha}-Glucosidase$; ${\alpha}-Amylase$ inhibitor;
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