• Title/Summary/Keyword: 조단백질

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Studies on the Preparation of Pheasant Meat Extracts by Protease (효소에 의한 꿩고기 가수분해물의 제조)

  • Jeong, Jae-Hong;Kim, Ki-Jun;Lee, Gyu-Hee;Lee, Seuk-Keun;Oh, Man-Jin
    • Korean Journal of Agricultural Science
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    • v.25 no.1
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    • pp.107-117
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    • 1998
  • Ths studies was carried out to investigate the processing possibility of pheasant meat extracts treated with proteases. The crude protein, aminonitrogen, degree of hydrolysis, yield and amino acid composition of pheasant meat extracts when it was treated with proteases at various temperature and reaction time were analyzed. The crude protein contents of pheasant meat extracts processed in $130^{\circ}C$ were more than when it was done in $100^{\circ}C$, but the contents of aminonitrogen were not quite different between two processing temperature. The content of crude protein and aminonitrogen when pheasant meat was hydrolyzed with protease NP and prozyme A. The yields of pheasant meat extracts, when pheasant meat were treated at $100^{\circ}C$ and $130^{\circ}C$, were from 2.24 to 7.10% and from 5.51 to 10.45%, respectively. And the yield of extraction depended on extraction temperature, kinds of enzyme, amount of enzyme, extraction time. The content of aminonitrogen in pheasant meat extracts treated with enzyme was much higher than any other treatments. And it depended on amount of enzyme, extraction time and temperature. The amount of the amino acids in pheasant meat extracts treated by protease NP were eminently higher than by heat at $100^{\circ}C$ or $130^{\circ}C$.

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Effect of Inoculation with Bradyrhizobium sp. and Lime Types on Peanut Plant at Newly Reclaimed Area II. Nutritional Content in Seed (야산개간지(野山開墾地)에서 근류균(根瘤菌) 접종(接種) 및 석회(石灰) 비종별(肥種別) 시용(施用)이 땅콩 생육(生育)에 미치는 영향(影響) II. 종실(種實)의 양분함량(養分含量))

  • Lee, Deog-Bae;Choi, Song-Yeol;Kwon, Ta-Oh;Im, Geon-Jae;So, Jae-Don
    • Korean Journal of Soil Science and Fertilizer
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    • v.24 no.4
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    • pp.286-292
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    • 1991
  • This study was carried out to investigate in influence of inoculation B. sp. and lime types on nutrient content of peanut seed, and the correlation between nutritient contents in seed and plant. 1. Inoculated B. sp., content of crude protein, phenylalanine, isoleucine, cystine, histidine, aspartic acid, serine, and alanine in seed were increased significantly, but that of crude fat was decreased. 2. The content of crude fat was the highest without lime, glutamic acid was the highest with calcium sulfate, valine, phenylalanine, isoleucine, leucine, lysine, aspartic acid, serine, glycine and alanine with slaked lime, and histidine with calcium carbonate. 3. The content of crude protein in seed was negatively correlated with that of crude fat. The contents of chlorophyll, allantoin, ammonia, free amino acid, T-N, $P_2O_5$, and CaO in leaf at 100 days after sowing were positively correlated with the content of crude protein and various kinds of amino acid in seed, but negatively with the content of crude fat. The content of nitrate in leaf at 100 days after sowing showed the result of contrary tendency.

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Changes in the Contents of Some Metabolites and Ions and in Some Enzyme Levels in Rice Plants Grown under Water-and Salt-stressed Condition (수분장애(水分障碍) 및 염장애(鹽障碍)하에서 수도체(水稻體)중 효소수준(酵素水準) 및 유기대사산물(有機代謝産物)과 무기(無機)이온 함량의 변화)

  • Park, Ro-Dong
    • Applied Biological Chemistry
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    • v.25 no.3
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    • pp.135-141
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    • 1982
  • Two rice cultivars, Jinju and Iri 348, were used to compare the changes in the contents of some organic metabolites and ions and in some enzyme levels under water-and salt-stressed conditions. The water loss and proline accumulation under water and salt stresses were accelerated more in the salt-sensitive cultivar Iri 348 than in the salt-tolerant Jinju. The contents of crude protein, total free amino acids, proline and polyphenols increased under water-or salt-stressed rice, but that of reducing sugar increased under water stress only. The water-and salt-stresses induced the high ratio of low molecular organic solutes to crude protein in Jinju but not in Iri 348. The ratio of total free amino acids to crude protein increased under the stressed conditions was likely due to high protease activity. The contents of $Na^+$ and $Cl^-$ were higher in Iri 348 than in Jinju. Iri 348 had higher values of $Na^+/Ca^{2+}$ and monovalent/divalent of cations, but lower of $K^+/Na^+$ than Jinju Rice. The further studies should emphasize to set the correlations between these ratios and tolerance to water and salt stresses among rice cultivars.

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Studies on the Quality and Productivity Improvement by Mixed Sowing of Oat-Hairy Vetch (연맥-헤어리베치 혼파에 의한 사료가치 및 생산성 향상 연구)

  • Kim, J.G.;Chung, E.S.;Yoon, S.H.;Seo, S.;Seo, J.H.;Park, G.J.;Kim, C.K.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.1
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    • pp.31-36
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    • 2002
  • The yield and quality of winter forage crops such as oats could increase by mixed sowing with a winter legume such as hairy vetch(Vicia villosa Roth). The purpose of this study was to improve the forage quality and productivity using Oat-Hairy vetch mixture in winter season for three years. The results of this experiments were summarized as follows. In mix seeding of oat and hairy vetch, the dry matter(DM) content of herbage was 15∼16% in fall-harvest and 19∼20% in spring-harvest. Crude protein(CP) content of hairy vetch was the highest as 30.1% and the mixture was higher than that of oat. Acid detergent fiber(ADF) and neutral detergent fiber(NDF) content of hairy vetch was lower, but in vitro dry matter digestibility(IVDMD) and total dry matter digestibility(TDN) content was higher than that of oat. Although oat showed the low yield by 3.7∼4.4 DM t/ha, the Fall-seeded-Spring-harvest hairy vetch plot showed highly production by 9.5 DM t/ha. Crude protein and TDN yield of Fall-seeded-Spring-harvest plots were higher than those of others. According to this study, the highest dry matter yield could be obtained by Fall-seeded-Spring-harvest. It would be recommend in cropping field for oat.

Effects of Feeding Total Mixed Rations Containing Different Winter Forage Crop Silages on Feed Intake, Nutrient Digestibility and Blood Characteristics in Korean Black Goats (동계사료작물 사일리지를 첨가한 TMR 급여가 흑염소의 사료섭취량, 영양소 소화율 및 혈액성상에 미치는 영향)

  • Jung, Gi-Woung;Jo, Ik-Hwan;HwangBo, Soon;Lee, Sung-Hoon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.29 no.4
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    • pp.389-398
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    • 2009
  • This study was conducted to determine the feed value of total mixed rations (TMR) containing different winter forage crop silages on feed intakes, daily gain, nutrient digestibility, and nitrogen retention in Korean black goats. A total of 12 male goats were used in this study and each goat was housed in individual metabolism crates. The treatments were four diets, consisting of whole crop barley silage (T1), rape silage (T2), rye silage (T3), and Italian ryegrass silage (T4). The experiment was designed as a $4{\times}4$ Latin square arrangement in three replicates. Daily intakes for dietary dry matter (DM), organic matter (OM), crude protein (CP) per head were highest in rape silage treatment, and lowest in whole barley silage (p<0.05). Digestible intakes for CP and acid detergent fiber were highest in rape silage treatment. The daily gain of rape, rye, and Italian ryegrass silages were significantly (p<0.05) higher than that of whole crop barley silage. Digestibility of DM. CP, fiber were highest in whole crop barley silage, and lowest in rape silage (p<0.05). Nitrogen (N) intake and N retention were significantly highest in rape silage and lowest in whole crop barley silage (p<0.05). The results showed that, among different winter forage crop silages, rape silage incorporation into TMR had the best performances indicating increases of feed intakes and N retention in Korean black goats.

Change of Dry Matter Yield and Feed Values According to Different Growth Stages of Italian Ryegrass and Triticale Cultivated in the Central Northern Region (중북부 지역에서 생육단계에 따른 이탈리안 라이그라스와 트리티케일의 건물수량과 사료가치)

  • Lee, Hong-Ju;Byeon, Ji-Eun;Hwang, Sun-Goo;Ryoo, Jong-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.1
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    • pp.50-56
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    • 2020
  • The field experiment was carried out to evaluate changes of dry matter productivity and feed value of Italian ryegrass and triticale at different growth stages in Wonju from 2018 to 2019. Italian ryegrass and triticale forages were harvested at an interval of seven days from May to June. The dry matter yield of Italian ryegrass with 7,870 kg/ha harvested on May 16 was significantly higher than that harvested on May 3 and May 9. The dry matter yield of triticale with 12,050 kg/ha harvested on June 7 was significantly higher than that harvested from May 3 to May 16. The crude protein yields harvested on May 16 were 890 kg/ha for Italian ryegrass and 1,103 kg/ha for triticale and were significantly higher than those harvested on May 3 and May 9. The total digestible nutrient yield(TDN) of Italian ryegrass with 5,223 kg/ha harvested on May 16 was significantly higher than that harvested on May 3 and May 9. The total digestible nutrient yield of triticale with 8,277 kg/ha harvested on June 7 was significantly higher than that harvested during May, but not different from that harvested after June 7. Considering the dry matter yield, crude protein yield and total digestible nutrient yield, it is suggested that the optimal harvesting times for Italian ryegrass and triticale seem to be May 16 and June 7, respectively.

Studies on the Nitrogen Fixation of Legumes I. Effects of nitrogen fertilization level on nitrogen fixation and growth of ladino clover (두과목초의 질소고정에 관한 연구 I. 질소시비수준이 라디노 클로버의 질소고정 및 생장에 미치는 영향)

  • Lee, Hyo Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.13 no.1
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    • pp.16-22
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    • 1993
  • Ladino clover stolen growing in grazed sward transplanted in 1/5,000a Wagner pot with two rows and were grown in green house with 12h lighting. Fifty days after transplanting the first cutting was made, the secound and 3rd cutting was made 20 days after each harvest. Treatment were nitrogen fertilization level of 2.5, 5.0 and 7.5 kg N/10a. Nitrogen was top dressed after transplanting and each cutting. Growing point, above and under ground DM yield, nitrogen fixation, crude protein of stolon, root and change of acetylene reduction activity after cutting were investigated. Result are as follows. 1. Growing point, DM production were heighst in 5 kg N at the first and 2nd harvest while 2.5 kg at 3rd cutting. Plants with 7.5 kg N/10a were dead at 3rd harvest. There were significant differences in growing point between treatment. 2 Nitrogen fixation of ladino clover were the heightest in 2.5 kg N pot at 1st cutting and as cutting and N fertilization were contiuned nitrogen fixation activity was lowered. 3. Leaf have more crude protein than that of stolen and root while plant crude protein increased by 2nd cutting as plant got more nitrogen fertilizer and again decreased in 3rd cutting. 4. Acetylene reduction activity were lowest at 3 week and recovered normal level at 5 week after cutting, also DM root was same trend.

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The Effects of Dietary Protein Level on Laying Performance and Egg Quality in Japanese Quail (메추리에 있어서 산란 사료 내 단백질 수준이 산란 성적과 난질에 미치는 영향)

  • Oh, Sung-Taek;Kim, Je-Hun;Park, Seung-Jae;Yun, Jeong-Geun;Zheng, Lan;An, Byoung-Ki;Kang, Chang-Won
    • Korean Journal of Poultry Science
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    • v.39 no.3
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    • pp.207-213
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    • 2012
  • This experiment was conducted to investigate the effects of various levels of dietary protein on laying performance and egg quality in laying quail. A total of six hundred forty 7-week-old laying type Japanese quail were divided into four groups with eight replicates per group (20 birds per replicate) and fed four diets differing in dietary protein levels (18, 20, 22 and 24%) with isocaloric corn-soybean meal-based diets for 8 weeks. The results showed no significant differences in feed intake and egg weight among the groups. With an increase in dietary protein levels, the egg production and daily egg mass were linearly increased (P<0.05). However, there were no significant differences in eggshell thickness and Haugh unit. The dietary protein did not affect the blood levels of GOT, GPT, BUN, albumin, and creatinine. The ammonia concentration of intestinal digesta was not affected by the dietary treatments. The contents of hepatic triacylglycerol in the groups fed diets containing 20% protein or more were significantly reduced as compared with that of 18% CP group (P<0.05). Overall, this study showed that relatively high levels of dietary CP are needed to sustain the higher egg production and daily egg mass and to avoid the hepatic triacylglycerol accumulation in Japanese quail.

Preparation of Yeast Extract from Waste Brewer's Yeast using Various Enzymes (각종 효소를 이용한 맥주 폐효모로부터 효모추출물 제조)

  • Lee, Ok-Hwan;Rhee, Seong-Kap;Son, Jong-Youn;Kim, Kyung-Im;Kim, Hyun-Duk;Lee, Boo-Yong
    • Korean Journal of Food Science and Technology
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    • v.34 no.5
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    • pp.867-872
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    • 2002
  • This study was performed to investigate the optimum process conditions for manufacturing yeast extract from waste brewer's yeast using various enzymes. Contents of IMP, GMP, free amino acids, and crude protein of yeast extracts were measured by enzymes treatment. Crude protein contents of yeast extracts subjected to cell wall digestion enzyme treatment were 21.1, 33.6, and 28.0% for the control grouup, glucanase (0.5%, 12 h), and tunicase (1%, 18 h), respectively. Crude protein contents of yeast extracts subjected to protease treatment were 22.0, 30.8, and 29.8% for control group, bromelin (1%, 3 h), and protamex (1%, 3 h), respectively. Crude protein content of yeast extract subjected to glucanase and protamex mixed treatment was 34.4%. The total contents of IMP and GMP of yeast extracts subjected to G+P+A (glucanase+phosphodiesterase+adenyldeminase) and G+Pro+P+A (glucanase+protamex+phosphodiesterase+adenyldeaminase) treatments were 1,066 and 1,047 mg/100 g, respectively. The content of free amino acids of yeast extract was the highest (2,302 mg/100 g) in G+Pro+P+A treatment. Optimum concentration and process condition of enzyme treatment to obtain yeast extract with high IMP, GMP, and free amino acid content were in the order of glucanase (0.5%, 12 h), protamex (1%, 3h), phosphodiesterase (0.1%, 3 h) and adenyldeaminase (1%, 1.5 h) treatments.

Effects of Glycine soja Supplementation on the Quality of Corn Silage (야생콩(Glycine soja)을 이용한 옥수수 사일리지의 품질향상)

  • Yang, Bung-Mo;Heo, Jung-Min;Park, Kee Woong;Lee, Hyung-Suk;Lee, Soo-Kee
    • Weed & Turfgrass Science
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    • v.5 no.4
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    • pp.241-246
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    • 2016
  • This study was conducted to examine the effects of Glycine soja (GS) supplementation on the quality of corn silage. Corn silage was used in a $3{\times}2$ factorial arrangements with respective factors being addition of GS (0, 10, and 20%) and without or with Lacobacillus plantarum as lactic acid bacteria, and were stored for 40 days at a room temperature ($20-25^{\circ}C$). Corn silage with supplemented 20% GS increased (p<0.05) crude protein contents compared with that in corn silage with supplemented 0 and 10% GS, however crude fat, NDF, and starch concentrations was not affected (p<0.05) by addition of GS. Furthermore, the interaction was found (p<0.05) between corn silage with supplemented 20% GS and the addition of lactic acid in butyric acid. Silage pH was increased while GS supplementation increased. Corn silage with supplemented 20% GS increased (p<0.05) glucose and fructose concentrations. The results of current study indicate that corn silage with supplemented 20% GS could be used as a useful strategy to improve corn silage with increased crude protein contents along with sucrose, glucose and fructose concentrations.