• Korean Journal of Environment and Ecology
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• v.27 no.4
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• pp.487-497
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• 2013

This study was conducted to suggest vegetation management plan for Gugok landscape maintenance and improvement by deducing the vegetation landscape factors inherent in Yongha Gugok and understanding vegetation structure through the investigate of existing vegetation and plant community structure of Yongha valley in Woraksan National Park. There were broad and flat rocks, natural layered stones, clear water, light stones, stream, valleys, waterfalls, Pinus densiflora and Acer pseudosieboldianum as a result of deducing natural factors on poetry. There were P. densiflora and A. pseudosieboldianum appeared as one of main vegetation landscape elements. The actual vegetation analysis results were as followed. The natural vegetation occupied 67.5% and it was classified as P. densiflora community, Quercus variabilis community, Q. variabilis-P. densiflora community, Q. variabilis-Q. serrata community, Q. serrata community, Q. mongolica community, Q. mongolica-P. densiflora community, Deciduous broad-leaved tree community. The artificial vegetation(18.7%) was classified as Q. serrata community-Larix kaempferi community, Q. mongolica- Castanea crenata community, L. kaempferi community, L. kaempferi-C. crenata community, fruticeta, L. kaempferi-Q. mongolica community. The grassland area(2.0%) was classified as Miscanthus sinensis community, Phragmites communis community, and other areas were classified as landscape tree planting area, farm, orchard, residential area. The representative vegetation were P. densiflora community, Q. variabilis-Q. serrata community, L. kaempferi community, Deciduous broad-leaved tree community in Yongha Gugok. The species diversity index of Shannon was 0.6274~0.9908 on the whole. Yongha Gugok, as a symbol of succession on confucianism and reverence for nature, should be preserved natural valley landscape being clean and wijungchuksa at the end of Joseon Dynasty and Japanese Colonial era. In this historical and cultural Gugok, vegetation landscape management plan is needed to landscape maintenance with P. densiflora community, density control with L. kaempferi community. And it is considered when natural disasters and artificial damages happened, P. densiflora-oriented vegetation restoration plan should be applied in order to restore.

• Journal of Life Science
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• v.22 no.9
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• pp.1152-1158
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• 2012

Development of transgenic plant increasing crop yield or disease resistance is good way to solve the world food shortage. However, the persistence of marker genes in crops leads to serious public concerns about the safety of transgenic crops. In the present paper, we developed marker-free transgenic rice inserted high molecular-weight glutenin subunit (HMW-GS) gene ($D{\times}5$) from the Korean wheat cultivar 'Jokyeong' using Agrobacterium-mediated co-transformation method. Two expression cassettes comprised of separate DNA fragments containing only the $D{\times}5$ and hygromycin resistance (HPTII) genes were introduced separately into Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring $D{\times}5$ or HPTII was infected into rice calli at a 3: 1 ratio of EHA105 with $D{\times}5$ gene and EHA105 with HPTII gene expressing cassette. Then, among 66 hygromycin-resistant transformants, we obtained two transgenic lines inserted with both the $D{\times}5$ and HPTII genes into the rice genome. We reconfirmed integration of the $D{\times}5$ and HPTII genes into the rice genome by Southern blot analysis. Wheat $D{\times}5$ transcripts in $T_1$ rice seeds were examined with semi-quantitative RT-PCR. Finally, the marker-free plants containing only the $D{\times}5$ gene were successfully screened at the $T_1$ generation. These results show that a co-infection system with two expression cassettes could be an efficient strategy to generate marker-free transgenic rice plants.

• Journal of Life Science
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• v.23 no.11
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• pp.1317-1324
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• 2013

High-molecular weight glutenin subunits (HMW-GS) have been shown to play a crucial role in determining the processing properties of the wheat grain. We have produced marker-free transgenic rice plants containing a wheat Glu-1Bx7 gene encoding the HMG-GS from the Korean wheat cultivar 'Jokyeong' using the Agrobacterium-mediated co-transformation method. The Glu-1Bx7-own promoter was inserted into a binary vector for seed-specific expression of the Glu-1Bx7 gene. Two expression cassettes comprised of separate DNA fragments containing only Glu-1Bx7 and hygromycin phosphotransferase II (HPTII) resistance genes were introduced separately to the Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Glu-1Bx7 or HPTII was infected to rice calli at a 3:1 ratio of Glu-1Bx7 and HPTII, respectively. Then, among 216 hygromycin-resistant $T_0$ plants, we obtained 24 transgenic lines with both Glu-1Bx7 and HPTII genes inserted into the rice genome. We reconfirmed integration of the Glu-1Bx7 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the wheat Glu-1Bx7 were stably expressed in the rice $T_1$ seeds. Finally, the marker-free plants harboring only the Glu-1Bx7 gene were successfully screened at the $T_1$ generation.

• FLOWER RESEARCH JOURNAL
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• v.19 no.4
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• pp.187-191
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• 2011

Rising soil temperature was effectively controled by shading net of 50% and cooled water to maintain average $18.8^{\circ}C$ (maximum $23^{\circ}C$), then shading net of 50% and ground water to maintain average $23.2^{\circ}C$ (max. $28.5^{\circ}C$), shading paint of 30% and shading net (50%) to maintain average $24^{\circ}C$ (max. $30^{\circ}C$) in that order. Alstroemeria 'Modena' most affected by shading and cooling water was better in shoot length as 95.9 cm and fresh weight as 67 g than those of other treatments. The production of cut flower was increased more 121% with treatment 50% shading net and cooling water, 59% with 50% shading net and ground water, and 65% with 30% shading paint than that of 50% shading net, respectively. Soil temperature was higher $8^{\circ}C$ with the plot of circulating warm water than untreated control plot. Alstroemeria 'Aspen', 'Modena', and 'Chanel' increased more plant growth such as plant height, fresh weight in warm water than in untreated control plot, but Alstroemeria 'Bordeaux' decreased plant height. Because of increasing plant growth, flower quality such as peduncle length, peduncle diameter, floret number and flower weight of 4 all cultivar was better in the plot of circulating warm water than untreated control plot. Also, the production of cut flower was increased the most in Alstroemeria 'Modena' by 38%, 'Aspen', 'Bordeaux', and 'Chanel' in that order.

• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.238-243
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• 2010

This study was conducted to investigate effects of nutrient solution pH control agent and foliar spray of ethephon on the reduction of stem inside-cavity and improvement of flower quality in chrysanthemum 'Baekma'. Changes of pH in nutrient solution as affected by the kind of pH control agent showed more settled pattern in the plot of KOH treatment than in others. Plant growth and development such as cut flower length, leaf numbers, cut flower weight and petal number were the greatest in the plot of KOH. However, the size and area of stem cavity was large in KOH rather than $KHCO_3$. As changes in the absorbing patterns of mineral elements as affected by nutrient solution pH control agent in closed system, contents of total nitrate, phosphorus, potassium and calcium by $KHCO_3$ was absorbed into the plant less than KOH, so that it remained a lot of mineral element residues rather than KOH in closed system. Plant growth as affected by the foliar spray of ethephon showed growth retardation effect in the plot of solution diluted to 1 : 500 and growth promotion effect in the plot of solution diluted to 1 : 1,000 or 1 : 2,000. The number of petals was the best in the plot of foliar spray of ethephon solution diluted to 1 : 1,000 before flowering at 45 days showing 331 petals compared to control showing 302 petals. The size and area of stem cavity as affected the foliar spray of ethephon was smaller 1 mm and 7%, respectively, in the plot of solution diluted to 1 : 2,000 before flowering at 30 days than in control. Therefore, treating pH stabilization using KOH after floral initiation stage with the foliar spray of ethephon solution diluted to 1 : 2,000 before flowering at 30 days would help to reduce stem cavity size and improve flower quality in hydroponically grown chrysanthemum 'Baekma'.

• Korean Journal of Environment and Ecology
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• v.33 no.2
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• pp.216-227
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• 2019

The purpose of this study is to propose the rational mountain stream management strategy considering the natural conditions and social needs of the Republic of Korea. We reviewed domestic and overseas studies related to mountain streams, identified the study areas by text mining and co-word analysis using the VOSviewer program, and then analyzed the spatial and temporal study trends and topics of each study area. The results showed that domestic studies on mountain streams are still in an initial stage compared to overseas studies. Overseas studies on mountain streams can be classified into four groups: (i) habitat and species composition of fish and invertebrates, (ii) hydrological phenomena and nutrient migration, (iii) transport of sediment and organic materials and the relevant morphological changes by runoff flows, and (iv) plant species composition in mountain streams. Of these study subjects, domestic studies belonging to the (i) group mainly focused on macroinvertebrates while domestic studies belonging to the (iii) group regarded transport of sediment and organic materials as not the ecological disturbance but the source of sediment-related disasters. We then analyzed the rate of each research group to all papers by period and country. The results showed that the overseas studies belonging to (iii) and (iv) groups have increased with time, and the increase was mostly due to the studies in the United States, Brazil, Canada, and China. On the other hand, domestic studies belonging to (i) and (iii) groups increased somewhat with time, but there was a slight lack of correlation between the two subjects. Therefore, the hybridity studies to complement the shortage is necessary for the future.

• Korean Journal of Environmental Biology
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• v.37 no.1
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• pp.42-47
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• 2019

This experiment was conducted to investigate the effects of pH on the mineral nutrient uptake and growth of the four Chinese chives species. The Chinese chives species used in the experiment were the cultivated species grown in the farm (cultivated Allium tuberosum) and three wild species of wild Allium tuberosum, A. thunbergii and A. senescens. The pH levels of soil medium were set to be 4.5, 6.5, and 7.5. Fresh weight(FW) of cultivated A. tuberosum was highest at all pH levels. The increase of soil pH increased the FW of the wild A. tuberosum and A. thunbergii, but no difference was noted for the A. tuberosum and A. senescens. Plant height was higher in the order of wild A. tuberosum, A. thunbergii, and cultivated A. tuberosum and A. thunbergii. Notably plant height of the wild A. tuberosum increased significantly by the pH increase. The Zn content of the wild A. tuberosum was shown to be significantly higher than that of the other species and increased with the increase of soil pH. This indicates that there is a close relationship between the plant height and Zn content in Chinese chives plant. Principal component analysis for characterizing closely related A. species using the factors of plant growth and amounts of nutrients uptake showed that the cultivated A. and wild A. tuberosum were in the $4^{th}$ quadrant of the graph which are classified as the same species, while A. senescens and thunbergii was in $1^{st}$ and $3^{rd}$ quadrant indicating different species, respectively.

• Journal of agriculture & life science
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• v.51 no.1
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• pp.57-67
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• 2017

We conducted this study to investigate vegetation structure and soil conditions of six Viburnum erosum Thunb.(V. erosum) populations which showed high dominance. A total of 68 woody plants were observed to distribute: 13 in tree layer; 38 in subtree layer; and 48 in shrub layer. Based on the Mean Importance Value(MIV), the dominant tree species in the V. erosum populations were as follows in order of: Acer pseudosieboldianum(Pax) Kom., Callicarpa japonica Thunb., Indigofera kirilowii Maxim. ex Palib., Lindera obtusiloba Blume, Prunus sargentii Rehder, Quercus variabilis Blume, Rhododendron mucronulatum Turcz. and Vaccinium oldhamii Miq. In paticular, the species observed in all of the study sites were Quercus serrata Murray and Styrax japonicus Siebold & Zucc. It was found that the presence of V. erosum was positively correlated with that of Albizia julibrissin Durazz. and Symplocos chinensis f. pilosa (Nakai) Ohwi, while negatively correlated with that of Platycarya strobilacea Siebold & Zucc. According to the result, means of species diversity(H'), maxim species diversity(H' max), evenness(J') and dominance(1-J') were measured at 0.887, 1.102, 0.803, and 0.196, respectively. The soil conditions properties of pH value, organic matter content, total nitrogen content, and the amount of available phosphorous indices in the study sites were 4.3, 10.6%, 0.34% and $0.68mg{\cdot}kg^{-1}$, respectively. Meanwhile, the total amount of exchangeable cations of $Ca^{2+}$, $K^+$, and $Mg^{2+}$ were $16.84cmol_c{\cdot}kg^{-1}$, $6.41cmol_c{\cdot}kg^{-1}$, and $4.26cmol_c{\cdot}kg^{-1}$, respectively. Overall, Viburnum erosum Thunb. populations tend to grow in the strongly acid soil, which has great abundance of soil organic matters, a lot of exchangeable cations, and has higher amount available phosphorous than that of nitrogen.

• Korean Journal of Environment and Ecology
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• v.35 no.6
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• pp.621-631
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• 2021

The purpose of this study is to survey and analyze acorn production and characteristics of the Quercus acuta Thunb. according to the need for information on seed supply and seedling cultivation during the restoration of warm broad-leaved forests. For the survey, a total of 30 seed traps with a surface area of 1 m² were set up, 3 in each of 10 quadrats (8 in Wando, 1 in Haenam, and 1 in Jindo). The acorns that fell in the seed trap at the end of each month were collected from August to December each year between 2013 to 2016. The collected acorns were then classified into sound, damaged, decayed, or empty grade, and the number of acorns produced was calculated. In the case of sound acorns, acorn traits, such as length, diameter and weight of acorns without cupule, were measured. Duncan's multiple tests of acorn production and characteristics were conducted for comparative analysis of the annual average values with the values by year, stand, month, and treatment plot. The annual number of acorn dropped into the seed traps in each quadrat from 2013 to 2016 was 5-350 acorns/3 m² in 2013, 17-551 acorns/3 m² in 2014, 5-454 acorns/3 m² in 2015, and 14-705 acorns/3 m² in 2016. There was a large difference in acorn production between the quadrats, presumably attributed to the difference in the amount of light received due to the density of trees in the square. Annual acorn production per area was 335,000 acorns/ha in 2013, 932,000 acorns/ha in 2014, 556,000 acorns/ha in 2015, and 1,037,000 acorns/ha in 2016. That was a sharp variation of acorn production in the two-year cycle. As the fluctuation in the production of Q. acuta showed simultaneity between stands, it is judged that Quercus acuta Thunb. had a clear cycle of fruitfulness and fruitiness between forest objects. September showed the biggest amount of fallen acorns and largest damage from insect pests, indicating that preventing early fall of acorns could increase the fruiting period and enable mass production of sound acorns. There was no significant difference between annual average acorn length in each region. In the case of the acorn diameter and weight, the average values of acorns from Haenam were significantly higher than those from Wando and Jindo. There was no significant difference in the average annual acorn characteristics by month, and the average annual acorn length, diameter, and weight in November were 19.72mm, 12.23mm, and 1.64g, respectively, the highest between August and November.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.32 no.3
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• pp.32-45
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• 2014

The study attempts to interpret original location and landscape composition of Yowol-pavilion Garden under the premise that tablet and poetry are important criteria for inference of unique location and landscape composition of a pavilion garden. The study raises the meaning, status, and value of Yowol Pavilion Garden as a cultural asset. The results of the study are as follows. First, Yowol-pavilion Garden was a place where famous Confucius scholars in Joseon Dynasty in 16th Century, including Kim, Kyung-Woo, the owner of the garden, used to share the taste for the arts and poetries with their colleagues. Along with a main characteristic of Yowol Pavilion Garden as a hideout for the Confucius scholars who stayed away from a political turmoil, the new place characteristic of the garden, a bridgehead for the formation of regional identity, was discovered in the record of "Joseon-Hwanyeo-Seungram Honam-Eupji JangSeong-Eupji", As described in "The first creative poetry of Yowol-pavilion", the intention for the creation of Yowol-pavilion Garden and the motive for its landscape composition is interpreted as a space of rivalry where the world, reality and ideals are mixed up. Second, related to outstanding scenic factors and natural phenomena when taking a view from the pavilion, the name of the house 'Yowol', which means 'Greeting the moon rising on the Mt. Wolbong' is the provision of nature and taste for the arts, and is directly connected to the image of leaving the worldly. In other words, the name was identified to be the one that reflected the intention for landscape composition to follow the provision of nature separating from joy and sorrow of the mundane world. Third, as for the location, it was confirmed through "YeongGwang-Soksu-Yeoji-Seungram" that Yowol-pavilion Garden was a place where the person who made the pavilion prepared for relaxation after stepping down from a government post, and literature and various poetry show that it was also a place of outstanding scenic where Yellow-dragon River meandered facing Mt. Wolbong. Especially, according to an interview with a keeper, the visual perception frequency of the nightscape of Yowol-pavilion Garden is the highest when viewing by considering the east, the direction of Yellow-dragon River, as Suksigak[normal angle's view], towards Yowel-pavilion from the keeper's house. In addition, he said that the most beautiful landscape with high perception strength is when the moon came up from the left side of Yowol-pavilion, cuts across the Lagerstroemia india heal in front of Yowol-pavilion, and crosses the meridian between Mt. Wolbong peaks facing Yowol-pavilion. Currently, the exposure of Yowol-pavilion Garden is $SE\;141.2^{\circ}$, which is almost facing southeast. It is assumed that the exposure of Yowol-pavilion Garden was determined considering the optimized direction for appreciating the trace of the moon and the intention of securing the visibility as well as topographic conditions. Furthermore, it is presumed that the exposure of Yowol-pavilion Garden was determined so that the moon is reflected on the water of Yellow-dragon River and the moon and its reflection form a symmetry. Fourth, currently, Yowol-pavilion Garden is divided into 'inner garden sphere' composed of Yowol-pavilion, meeting place of the clan and administration building, and 'outer garden sphere' which is inclusive of entrance space, Crape Myrtle Community Garden and Pine Tree Forest in the back. Further, Yowol-pavilion Garden has been deteriorated as the edge was expanded to 'Small lake[Yong-so] and Gardens of aquatic plants sphere' and recently-created 'Yellow-dragon Pavilion and park sphere'. Fifth, at the time it was first made, Yowol-pavilion Garden was borderless gardens consisting of mountains and water taking a method of occupying a specific space of nearby nature centering around pavilion by embracing landscape viewed from the pavilion, but interpreted current complex landscapes are identified to be entirely different from landscapes of the original due to 'Different Changes', 'Fragmentation' and 'Apart piece' in many parts. Lastly, considering that Yowol-pavilion Garden belongs to the Cultural Properties Protection Zone, though not the restoration to the landscapes of the original described in tablet and literature record, at least taking a measure from the aspect of land use for minimizing adverse effect on landscape and visual damage is required.