• Title/Summary/Keyword: 제3종

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Studies on the Hemolytic Activities of Korean Wild Mushrooms (III) - Hemolytic Characteristics and in vivo Toxicity of Hemolysin of Hebelma crustuliniforme - (한국산 야생버섯의 용혈작용에 대한 연구 - 제 3보 : 무우자갈버섯(Hebeloma crustuliniforme) 용혈독소의 용혈특성 및 in vivo 독성 -)

  • Yang, Hee-Jung;Lee, Ji-Seon;Chung, Kyeong-Soo
    • The Korean Journal of Mycology
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    • v.30 no.2
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    • pp.119-123
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    • 2002
  • In the previous studies, we surveyed 68 Korean wild mushrooms for their hemolytic activity and found that cold-water extract of Hebeloma crustuliniforme contained heat-resistant hemolysin. In this study, partially purified hemolysin of the mushroom was obtained by cold-water extraction followed by precipitation with ammonium sulfate, solubility fractionation and then dialysis. The hemolysin was found to be > 12,000 in molecular weight and its optimal hemolytic temperature was $37^{\circ}C$ and it's hemolytic activity, on washed erythrocytes and unwashed erythrocytes, respectively, was in the order of sheep > rat > human ${\geq}$ mouse > chicken and sheep > mouse > human ${\geq}$ rat > chicken. When ip injected into ICR mice at 1.38 mg/kg, it incurred prompt hemolysis as well as severe renal toxicity and hepatotoxicity. These results strongly suggest that the toxicity of Hebeloma crustuliniforme, which had been well-known as a toxic wild mushroom, may be at least partly due to its hemolysin.

Assessment of Natural Environment - I. Selection of Plant Taxa - (자연환경 평가 - I.식물군의 선정 -)

  • 김철환
    • Korean Journal of Environmental Biology
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    • v.18 no.1
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    • pp.163-198
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    • 2000
  • This study aim was to propose plant taxa as an alternative proposal for the purpose of solving many kinds of difficulties occurred in the floral item of environmental impact assessment (EIA) used to date. Plant taxa presented here for environmental assessment can probably be useful in understanding the excellence of natural environment to specific regional space as well as in determining the order of priority as to species preservation. These taxa, selected by the floristic analysis with distributional range, can be classified into five degrees: the fifth degree, characterized by the discontinuous and/or isolated distribution, includes 83 taxa, 76 genera, 41 families; the fourth degree, occurred in only one subprovince as southern and northern plants, includes 314 taxa, 217 genera, 78 families; the third degree, two subprovinces as southern and northern plants, includes 307 taxa, 223 genera, 93 families; the second degree, generally distributed more than 1,000 meter in altitude, a whole subprovince, includes 109 taxa, 92 genera, 43 families; the first degree, at least three subprovinces, includes 258 taxa, 207 genera, 91 families. The taxa mentioned here environmental assessment, therefore, summed up to 1,071 taxa, 559 genera, 142 families from the Korean flora. It is suggested that the taxa belonging to the fifth degree might structually have by far extinctive possibility in respond to environmental damage comparing with other degrees, simply because these have narrow distributional ranges. [Floristics, Korean flora, Geographical distribution, Natural environment, Environmental assessment, Plant taxa].

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Recurrent Extraventricular Neurocytoma with Malignant Glial Differentiation - Case Report - (악성신경교 분화를 보이는 재발성 뇌실외 신경세포종 - 증례보고-)

  • Chang, In-Bok;Park, Se-Hyuck;Hwang, Hyung-Sik;Kim, Duck-Hwan;Nam, Eun Sook;Cho, Byung-Moon;Shin, Dong-Ik;Oh, Sae-Moon
    • Journal of Korean Neurosurgical Society
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    • v.30 no.4
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    • pp.522-527
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    • 2001
  • We present a case of recurrent extraventricular neurocytoma with malignant glial differentiation in left temporoparietal area. A 37-year-old man with presentation of generalized seizure had undergone biopsy of brain tumor in left parietal area in 1987, which revealed extraventricular neurocytoma and radiotherapy was followed. Postoperative course was uneventful until eleven years after biopsy, when he became gradually aphasic and right hemiplegic. Brain CT and MRI revealed enlargement of tumor with peritumoral edema and calcifications. He underwent subtotal tumor removal in 1998. Microscopic examination of second biopsy specimen revealed presence of large areas composed of anaplastic glial cells with frequent mitosis, nuclear pleomorphism, large eosinophilic cytoplasm and eccentric nuclei, resembling gemistocytes, which were strongly immunoreactive to glial fibrillary acidic protein(GFAP) but not to synaptophysin(SNP). Also focal areas of neuronal cells were found, which were immunoreactive to SNP but not to GFAP. These histologic findings imply that this recurred tumor was a high grade, mixed tumor with divergent differentiation of neuronal and astrocyte lineage. We report a rare case of extraventricular cerebral neurocytoma with malignant glial differentiation with review of the literature.

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An Empirical Analysis of the Regulation Effects on Webboard Games using VECM (벡터오차수정모형을 활용한 웹보드게임 규제영향에 대한 탐색적 연구)

  • Yoo, Byungjoon;Jeon, Seongmin
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.9 no.6
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    • pp.109-115
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    • 2014
  • Sales of online games companies that began from the small and medium-sized ventures, have grown to billions or hundreds of million dollars to target the global market. The issues related with industrial policies and regulations for game industry gain attentions. In particular, the Korean government has strengthened the relevant regulations of the webboard game service across many departments within the government such as Media Rating Commission, Game Products Administrative Committee, Ministry of Culture, Tourism and Sports, and Ministry of Female and Family. In this study, we analyze the effects of government regulation on webboard games using a VECM(Vector Error Correction Model). We have acquired the Gametrix time-series data during a year since July 2013. Having the co-integration estimated in the analysis process, we attempt to identify the long-term equilibrium relationship within webboard game industry and predict use time in near future. The results show that the use time has decreased to a third to a fourth comparing to the initial value at the beginning point in 2013. Two representative webboard games are exposed to the significant risks to have less or no use time. Additionally, we discuss the issues of the overall game industry influenced by the changes of webboard games.

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Comparison of Seed Germinating Vigor, Early Germination Characteristics, Germination Speed and Germination Peak Time in New Varieties of The Third Generation of Creeping Bentgrass Under Different Growing Conditions (생육환경에 따른 제3세대 크리핑 벤트그래스 신품종의 종자 발아력, 초기 발아 특성, 발아세 및 발아 피크 기간 비교)

  • Kim, Kyoung-Nam;Jung, Ki-Wan
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.11 no.5
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    • pp.79-91
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    • 2008
  • Research was initiated to investigate germination characteristics of creeping bentgrass (CB, Agrostis palustris Huds.). Seven varieties of CB were evaluated with different experiments. An alternative environment condition requiring for a CB germination test by International Seed Testing Association (ISTA) was applied in the Experiment I, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$(ISTA conditions). Experiment II was conducted under a room temperature condition of 5 to $25^{\circ}C$(natural conditions). In each experiment, data such as seed germinating vigor, early germination characteristics, germination speed and germination peak time were measured. Significant differences were observed in seed germinating vigor, early germination characteristics, germination speed and germination peak time. Seed germinating vigor was variable with different environments and varieties. It was 61.50 to 98.25% under ISTA conditions and 55.00 to 98.50% under natural conditions. There were considerable variations in early germination characteristics among CB varieties according to different conditions. Early germination characteristics indicated that all varieties were 1 to 4 days faster in germination under ISTA conditions, when compared with natural conditions. The germination speed, measured as days to seed germination of 70% and 90%, was much faster with Penn A-1, Penn A-4 and Penncross under ISTA conditions. But it was even faster with L-93 and Penncross when grown under natural conditions. Differences were also observed in germination peak time with varieties and growing conditions. It was 0.57 to 2.86 days under ISTA conditions and 0.74 to 1.74 days under natural conditions. Regardless of the environment conditions, the shortest variety was L-93 and the longest one T-1. Considering germinating vigor, early germination characteristics, germination speed and germination peak time, Penn A-1, Penn A-4 and Penncross were regarded as excellent varieties under ISTA conditions in terms of early establishment characteristics, while L-93, Penn A-1 and Penncross under natural conditions. These results suggest that an intensive germination test be needed prior to planting, for the early germinating vigor, germination speed and germination peak time. Also, a proper variety selection and comprehensive site analysis for the growing environmental conditions should be done before golf course construction.

Effects of the moisturizing cream containing yellowish natural colorants on the skin improvement (황색계 천연색소 추출물이 함유된 보습크림의 피부개선 효과)

  • Suh, Hwa-Jin;Kim, Yeon-Soon;Park, Sanggyu;Park, Shin
    • Journal of Applied Biological Chemistry
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    • v.60 no.2
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    • pp.155-159
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    • 2017
  • This study was to evaluate the effect of the moisturizing cream containing five kinds of plants extracts for human facial improvements such as hydrating ability, transepidermal water loss and erythema reaction. The antioxidant activities of yellowish natural colorants, extracted from five kinds of plants (Ceramium kondoi, Phellodendron amurense, Carthamus tinctorius, Gardenia jasminoides for. grandiflora and Aphis chinensis) were tested. Twenty-one volunteers were subjected to analyze the effects, and each result was determined by treating the complex cream containing plants extracts on their skin in three times. The moisture content on skin was significantly increased more than 50% by treating the cream containing five kinds of plants extracts. The transepidermal water loss was reduced and the level of the skin erythema reaction was improved after treatment of the complex cream. Survey analysis showed that the volunteers had highly satisfaction on the tested creams. The results suggest that the complex cream containing five plants extracts had an improved effect on facial skin, and therefore can be used as moisturizing ingredients in the related industry.

Studies on the Thermal Properties of Foods -I. Thermal Properties of Some Korean Foods- (식품(食品)의 열특성(熱特性)에 관(關)한 연구(硏究) -제(第) 1 보(報) : 한국고유식품(韓國固有食品)의 열특성(熱特性)-)

  • Chang, Kyu-Seob;Chun, Jae-Kun
    • Korean Journal of Food Science and Technology
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    • v.14 no.2
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    • pp.112-121
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    • 1982
  • Thermal properties of 12 Korean foods were determined by using an apparatus constructed by authors. Specific heat of acorn mook showed the highest value $(0.856\;kcal/kg{\cdot}^{\circ}C)$ and that of Chungkookjang the lowest value $(0.606\;kcal/kg{\cdot}^{\circ}C)$ among 12 foods investigated. Thermal conductivity of the salted wild sesame leaf at the initial temperature of $20^{\circ}C$ was found to be the highest $(0.451\;kcal/m{\cdot}h{\cdot}^{\circ}C)$ among the foods investigated and that of mungbean mook the lowest$0.304\;kcal/m{\cdot}h{\cdot}^{\circ}C$. Thermal conductivity appeared to get higher as the initial temperature increased from $20^{\circ}C\;to\;60^{\circ}C$. Thermal diffusivity of Kochujang was the highest $(0.0877\;cm^2/min)$ and the mungbean mook the lowest($(0.0518\;cm^2/min)$ among the foods investigated. Thermal conductivity of foods also appeared to increase as the initial temperature increased. The difference between the calculated values and the measured values was very small (1.655 residual percent in the statistical analysis).

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Epidemiological Observation on Recent Outbreaks of Canine Distemper in Korea (최근 국내발병 개 디스템퍼에 대한 역학적 조사)

  • Doo, Kim;Ji-young, Park;So-jeo, Ahn;Seok-young, Jeoung;Son-il, Pak
    • Journal of Veterinary Clinics
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    • v.21 no.3
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    • pp.229-235
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    • 2004
  • To characterize the recent outbreaks of canine distemper (CD) in Korea, we carried out epidemiological investigations by clinical observations, serum neutralizing (SN) antibody titer determination and RT-PCR on the 315 dogs which were clinically suspected as canine distemper virus (CDV) infection. One hundred and sixty two of 315 dogs were infected with CDV. Breed or gender did not seem to have effects on the prevalence of CD. The major part of dogs were in young age from 6 weeks to 18 weeks of age, and were not vaccinated or incompletely vaccinated. Clinical signs of dogs with CD were multi systemic and extremely variable. Dogs died from CD had significantly more ocular signs and neurologic signs than those of dogs survived (p<0.05). The SN titers against CDV of 157 (96.9%) dogs were under 1:16, which is less than protective level. One possible explanation for recent outbreaks of CD in Korea might be low antibody titers against CDV because of vaccination failure. Therefore, to reduce the impact of virulent infection in the dog population, dogs should be vaccinated adequately and prophylactic measures should include isolation of young dogs from the dog population until vaccination can be expected to provide protection.

Studies on Weed Control in Transplanted Bed of Pinus koraiensis S. et. Z and Larix leptolepis Gordon (잣나무와 일본잎갈나무 상체상(床替床)에 있어서 잡초방제(雜草防除)에 관(關)한 연구(硏究))

  • Ahn, Y.H.;Chung, J.C.;Han, S.S.
    • Korean Journal of Weed Science
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    • v.5 no.2
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    • pp.211-218
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    • 1985
  • To establish the weed control in transplanted bed of Pinus koraiensis and Larix leptolepis, alachlor, simazine, terbutryn, pendimethalin, oxyfluorfen and amitrol/2,4-D/methabenthiazuron (ustinex) were used by soil treatment and foliage application at 7days after transplantion of seedling. From the of soil treatment, grasses such as Dimeria ornithopoda, Alopecurus aequalis, Agropyron tsukushiense and Setaria verticillata were effectively controVed by 85 to 90% at the respective recommended rate of oxyfluorfen, alachlor and terbutryn. At the application rate of recommentation simazine, oxyfluorfen, pendimethalin and terbutryn respectively controlled 70 to 80% of broad-leaved weeds such as Erigeron annuus, Portulaca olearcea, Cerastium arvense, Capsella bursa-pastoris, Commelina commuis, Chenopodium hybridum and Stellaria alsine. Ustinex and oxyfluorfen were very effective for the control of perennials such as Artemisia princeps and Calystegia japonica. Initial sympton of phytotoxicity and decrease of growth in P. koreaiensis and L. leptolepis were not found by soil treatment and those in P. koraiensis was not shown by foliage application of all tested herbicides. But L. leptolepis foliage-applied with ustinex, oxyfluorfen, terbutryn and pendimethalin was great in early phytotoxicity and severe in growth inhibition.

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Allelopathic Potential Evaluation of Rice Varieties on Echinochloa crus-galli (돌피(Echinochloa crus-galli)에 대한 벼품종의 알레로파시 잠재성 평가)

  • Chung, Ill-Min;Kim, Kwang-Ho;Ahn, Joung-Kuk;Ju, Ho-Jong
    • Korean Journal of Weed Science
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    • v.17 no.1
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    • pp.52-58
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    • 1997
  • Rice (Oryza sativa L.) contains water-soluble substances that inhibit the germination of Echinochloa crus-galli. This study investigated the allelopathic potential of rice in which rice had been grown with E. crus-galli in the field. Extracts of forty-seven rice varieties were screened for allelopathic potential in the laboratory. Double distilled water was used as a control. Based on the germination percentage, the varieties may be classified in the following order of decreasing inhibition : Namweon(36%) and Gyehwa(38%) hulls extracts and Sambaeg(43%) and Seoan(46%) leaves extracts. In the comparison of allelopathic activity test between leaves and hulls extracts, hulls extracts contain more allelopathic toxic substances. In the effect of concentration treatment on the six levels of extract solutions(3, 6, 12, 25, 50, 100%, v/v) germination percentage was significantly inhibited as the extract concentration increased. The highest concentration(100%) caused the greatest reduction in E. crus-galli. Allelopathic potential effects were significantly different among the various varieties and between '94 and '95 year extracts. The results indicate that there are genetic differences among varieties for allelopathic potentiality on E. crus-galli. Allelopathic potential in the ecological aspects can be decided through variety selection.

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