• Title/Summary/Keyword: 제 4기 후기

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Characterization and Culture Optimization of an Glucosidase Inhibitor-producing Bacteria, Gluconobactor oxydans CK-2165 (α-Glucosidase 저해제 생산 균주, Gluconobacter oxydans CK-2165의 특성 및 배양 최적화)

  • Kim, Byoung-Kook;Suh, Min-Jung;Park, Ji-Su;Park, Jang-Woo;Suh, Jung-Woo;Kim, Jin-Yong;Lee, Sun-Young;Choi, Jongkeun;Suh, Joo-Won;Lee, In-Ae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.11
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    • pp.5179-5186
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    • 2012
  • Miglitol, a well-known therapeutic intervention agents for diabetes, exhibits competitive inhibitory activity against ${\alpha}$-glucosidase and it is usually produced through three sequential steps including chemical and bioconversion processes. Gluconobactor oxydans (G. oxydans) belonging to acetic acid bacteria biologically, converts 1-deoxy-1-(2-hydroxyethylamino)-D-glucitol (P1) into a key intermidiate, 6-(2-hydroxyetyl) amino-6-deoxy-${\alpha}$-L-sorbofuranose (P2) by incomplete oxidation. In this study, we identified and optimized fermentation conditions of CK-2165, that was selected in soil samples by comparing the bioconversion yield. CK-2165 strain was found to be closely related to G. oxydans based on the result of phylogenetic analysis using 16S rDNA sequence. Utilization of API 20 kits revealed that this strain could use glucose, mannose, inositol, sorbitol, rhamnose, sucrose, melibiose, amygdalin and arabinose as carbon sources. The culture conditions were optimized for industrial production and several important factors affecting bioconversion rate were also tested using mycelial cake. Cell harvested at the late-stationary phase showed the highest bioconversion yield and $MgSO_4$ was critically required for the catalytic activity.

Weed Occurrence and Yield of Rice in Transplanting Rice Culture with Paper Mulching (종이멀칭 이앙재배에서 잡초발생과 벼의 생육 및 수량)

  • Lee, B.W.;Cui, R.X.;Lee, H.L.
    • Korean Journal of Weed Science
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    • v.17 no.4
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    • pp.368-374
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    • 1997
  • Investigated ware the decomposition of mulch paper, weed occurrence, and rice growth and yield in transplanting rice culture with paper mulching in 1996 and 1997. The mulch paper were developed from domestic old corrugated container. In the experiment of 1996 where 25 percent of nitrogen fertilizer was applied as tillering fertilizer at 15 days after transplanting, it took about 28 to 40 days for the mulch paper to reach 50 percent decomposition according to types of mulch paper and fertilizer amount. The decomposition was faster in 18kg N/10a fertilization plot than in 12kg N/10a fertilization plot, and slower in the mulch paper treated with polyamidepolyamine epichlorohydrin resin(PPE) for the reinforcement of wet strength than in the mulch paper without PPE. In the experiment of 1997 where nitrogen fertilizer was not applied at tillering stage and instead 70 percent of nitrogen fertilizer was supplied as basal fertilizer, it took more than 42 days for the mulch paper(PPF treated) to reach 50 percent decomposition. Paper mulching was found to be very effective in controlling the paddy weeds at early stage of rice growth, but the efficacy, of weed control decreased especially in the mulch paper without PPE at later stage of rite growth due to the occurrence of Ludwigia prostrata in 1996. Few weeds occurred even in non-mulched plot without herbicide in 1997, rending difficulties in evaluating the efficacy of mulch paper in weed control, However, it could be deduced from the durability of mulch paper that paper mulching would have had sufficient efficacy of weed control. Rice yield was only 185kg/10a in the plot without mulching and weed control, but 657kg/10a and 771kg/10a in the paper(PPE treated) mulching plot with 12kgN/10a and 18kgN/10a, respectively, in 1996. The paper mulching plot showed rice yield similar to the weed control plot with herbicide in 1997. It would be concluded that paper mulching could ensure effective weed control and as high yield as the conventional rice cultivation with herbicidal weed control if the corresponding amount of basal nitrogen fertilizer is increased instead of omitting the nitrogen fertilizer at tillering stage.

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지화학적 방법을 이용한 울릉분지 코어퇴적물의 층서와 퇴적환경 연구

  • 박명호;김지훈;류병재;이성숙;한상근
    • 한국석유지질학회:학술대회논문집
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    • 2005.09a
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    • pp.94-100
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    • 2005
  • 동해 울릉분지 북서부해역에서 채취한 코어퇴적물을 이용하여 울릉분지의 제4기 후기 층서와 퇴적환경을 연구하였다. 이를 위하여 테프라층서, 탄소연대측정, 퇴적상, 퇴적물의 암질, 퇴적물과 공극수의 원소성분 등을 분석하였다. 퇴적상을 기준으로 코어퇴적물은 Unit 1, Unit II 및 Unit III으로 나누어지며, Unit의 경계부근에서는 주로 화산력으로 구성된 2매의 향암질 또는 조면암질 테프라층과 주로 화산재로 구성된 1매의 유문암질 테프라층이 협재되어 나타난다. 이들 테프라층에는 울릉도에서 기원한 울릉-오키층(Unit I/II 경계, 약 8.1 cal. kyr BC)과 울릉-야마토층(약 30.7 cal. kyr BC), 일본에서 기원한 아이라-탄자와층(Unit I/II경계, 약 22.8 cal. kyr BC)이 있다. Unit II의 중간부근에서 발견되는 DLM(Dark Laminated Mud) 층에는 망간보다는 철이 상대적으로 풍부하고, 철은 주로 구상집적체의 황화철로 발견되는데, 이들 광물은 정체환경 동안 물의 순환이 약화된 시기에 무산소환경에서 형성되었다. 퇴적물 내 유기물은 해양성 기원으로, 열적 성숙단계가 대부분 미성숙단계에 놓여 있다. Termination I 전에 급격하게 총유기탄소 (TOC)의 함량이 증가함을 보여주는데, 이는 빙기에서 간빙기로 변하는 시기에 해수면 변동과 관련이 있는 것으로 보인다. 공극수와 공기층 가스의 분석에 의하면, 황산염($SO_4^{2-}$) 농도는 매몰심도와 함께 증가하고, 반대로 공기층 가스의 주성분인 메탄($CH_4$)의 농도는 감소한다. 이는 미생물학적인 활동과 관련된 황산염 환원작용이 코어 상부에서 일어났으며, 점차 메탄생성단계 (methanogenesis)로 전이가 일어났음을 의미한다.측해본 결과 침몰선체의 강도는 초기강도에 비해 약 2/5정도 감소하였음을 알 수 있었다.할 수 있었다. 이 연구 사례를 통하여 3차원 GPR 탐사 또한 국부적인 이상대의 규명뿐만 아니라 광역적인 고고학 조사에도 다른 물리탐사와 마찬가지로 쉽게 활용될 수 있다는 결론을 얻을 수 있었다. 3차원 GPR 탐사가 향후 국내의 문화재 조사에 표준화된 탐사과정 중의 하나로써 적극 활용되길 기대한다.larity가 높은 oil에서는 약 $70 {\~} 90\%$의 phenoxyethanol이 유상에 존재하였다. 또한, 미생물에 대한 항균력도 phenoxyethanol이 수상에 많이 존재할수록 증가하는 경향을 나타내었다. 따라서, 제형 내 oil tomposition을 변화시킴으로써 phenoxyethanol의 사용량을 줄일 수 있을 뿐만 아니라, 피부 투과를 감소시켜 보다 피부 자극이 적은 저자극 방부시스템 개발이 가능하리라 보여 진다. 첨가하여 제조한 curd yoghurt는 저장성과 관능적인 면에서 우수한 상품적 가치가 인정되는 새로운 기능성 신제품의 개발에 기여할 수 있을 것으로 사료되었다. 여자의 경우 0.8이상이 되어서 심혈관계 질환의 위험 범위에 속하는 수준이었다. 삼두근의 두겹 두께는 남녀 각각 $20.2\pm8.58cm,\;22.2\pm4.40mm$으로 남녀간에 유의한 차이는 없었다. 조사대상자의 식습관 상태는 전체 대상자의 $84.4\%$가 대부분이 하루 세끼 식사를 규칙적으로 하고 있었으며 식사속도는 허겁지겁 빨리 섭취하는 경우가 남자는 $31.0\%$, 여자는 $21.4\%$로 나타났고 이들을 제외한 나머지 사람들은 보통 속도 혹은 충분한 시간을 가지고 식사를 하였다. 평소 식사량은 조금 적게

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A Study of School Science Textbooks which was used from 1906 to 1915 in Korea (대한제국 후기부터 일제 식민지 초기(1906-1915년)까지 사용되었던 과학교과용 도서의 조사 분석)

  • Park, Jong-Seok;Chung, Byung-Hoon;Pak, Sung-Jae
    • Journal of The Korean Association For Science Education
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    • v.18 no.1
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    • pp.93-108
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    • 1998
  • This study investigated science textbooks used from 1906 to 1915 in Korea, which is often neglected in previous researches. By doing so, more practical and specific understanding of the science education during that period can be made. It was based on the historical achieves "School Textbook List"(Vol. $4{\sim}9$) which was published by the Ministry of Education and the Government General of Chosen and other sources. The results and conclusions of the research are as follow; (1) The science textbooks which have been used from 1906 to 1915 in Korea can be categorized into three different tapes of books, either written in Chinese, or in Japanese, or in Korean. The names of science textbooks were Nature Study, Physics, Chemistry, Natural History, Hygienics, Physiology, Zoology, Botany, Astronomy, Physiography, Mineralogy, Geology. These names were much broader than those in curriculum settled by the government and rather correspond to the names of science textbooks in curriculum which settled by private schools. Therefore those science textbooks had been mainly used in private schools. Moreover almost all of the science textbooks published in Korea have started to appear after 1906. Since then many schools were newly opened and the number of students increased. It is possible to say that substantial science education in Korea established after 1906. (2) Science textbooks from 1906 to 1915 printed in Korea were controlled in their use by the Ministry of Education and the Government General of Chosen. They were the main means of government regulations, supported by Private School Ordinance, Regulations for Official Examination of textbooks in 1908 and Law of Publication in 1909. According to the result of official examination, as the time went by under the Japanese ruling of Korea, the increasing number of science textbooks were getting banned. While the science textbooks had enjoyed more freedom than the other textbooks from the control by inspection of the Government General of Chosen, the situation has been significantly changed as Japan started to intensity the control of all kinds of textbooks in Korea. Although there were a lot of copied science textbooks, 62 science textbooks printed in Korea, 72 Japanese science textbooks were used in Korea, and 40 Korean were to be engaged in science education compiling and copying science textbooks. There developments in science textbooks alone suggest that there were enormous amount of potentials in Korean science education at that time. However, all of these effects and progresses were destroyed when the sovereign authority of Korea was lost to Japan in 1910.

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Effects of Complex Direct-Fed Microbial Supplementation on Growth Performance and Nutrient Digestibility for Broilers (복합생균제의 급여가 육계의 성장능력과 영양소 소화율에 미치는 영향)

  • 김인호;손경승;홍종욱;권오석;민병준;이원백;박용하;이인선;한영근
    • Korean Journal of Poultry Science
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    • v.31 no.2
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    • pp.85-91
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    • 2004
  • This study was conducted to investigate the effects of dietary direct-fed microbials(DFM) on the growth performance and nutrient digestibility in broiler chickens. A total of two hundred eighty eight broiler chickens were randomly allocated into six treaments with four replications and fed for five weeks. Dietary treatments included 1) CON (basal diet), 2) DFM-1(basal diet + 0.2% Enterococcus sp. & Lactobacillus reuteri), 3) DFM-2(basal diet + 0.2% Enterococcus sp. & Lactobacillus plantarum), 4) DFM-3(basal diet + 0.2% Enterococcus sp. & Lactobacillus reuteri & Lactobacillus plantarum), 5) DFM-4(basal diet + 0.2% Enterococcus sp. & Lactobacillus reuteri & Lactobacillus plantarum & Yeast), 6) DFM-5(basal diet + 0.2% Enterococcus sp. & Lactobacillus reuteri & Lactobacillus plantarum & Bacillus subtilis). During the period of 1∼3weeks, average daily gain (ADG) and Feed/Gain were not significant different among treatments. In the later(3∼5weeks) and overall period(l∼ 5weeks), ADG and Feed/Gain tended to be improved in DFM treatments compared to the control. However, there were no significant differences among DFM complexes and between control and DFM treatments. The broilers fed DFM-3 diet was showd poor growth performance compared to the broilers fed DFM-l diet and similar to the broilers fed DFM-2 diet but there was no significant differences. The treatments of DFM-4 diet added Yeast and DFM-5 added Bacillus subtilis were improved in ADG and Feed/gain but no significant differences were found and also there was no specific DFM treatments in experiment period. In digestibility, the broiler chicks fed DFM treatments tended to improve N digestibility compared to control treatment. However, they were not significantly different among the treatments. In conclusion, DFM tended to improve growth performance in later stage of broiler.

Late Quaternary Depositional Processes in the Korea Plateau and Ulleung Interplain Gap, East Sea (동해 한국대지 및 울릉 분지간통로의 제4기 후기 해저퇴적작용)

  • 윤석훈;박장준;한상준
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.2
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    • pp.187-198
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    • 2003
  • High-resolution (Chirp, 3-11 kHz) echo facies and sedimentary facies of piston-core sediments were analyzed to reveal the late Quaternary depositional processes in the Korea Plateau and Ulleung Interplain Gap. The Korea Plateau is an Isolated topographic high with a very restricted input of terrigenous sediments, and its slope is characterized by a thin sediment cover and various-scale submarine canyons and valleys. Echo and sedimentary facies suggest that the plateau has been moulded mainly by persistent (hemi) pelagic sedimentation and intermittent settling of volcanic ashes. Sediments on the plateau slope and steep margins of ridges and seamounts were reworked by earthquake-induced, large-scale slope failures accompanied by slides, slumps and debris flows. As major fraction of the reworked sediments consists of (hemi) pelagic clay particles, large amounts of sediments released from mass flows were easily suspended to form turbid nepheloid layers rather than bottom-hugging turbidity currents, which flowed further downslope through the submarine canyons and spreaded over the Ulleung Basin plain. In the Ulleung Interplain Gap, sediments were introduced mainly by (hemi) pelagic settling and subordinate episodic mass flows (turbidity currents and debris flows) along the submarine channels from the slopes of the Oki Bank and Dok Island. The sediments in the Ulleung Interplain Channel and its margin were actively eroded and reworked by the deep water flow from the Japan Basin.

Residues of Benomyl and Bitertanol in Apples Treated as Postharvest Fungicides under Different Storage Conditions (수확후 처리제로서 Benomyl과 Bitertanol이 처리된 사과의 저장조건에 따른 잔류량)

  • Lee, Eun-Joo;Kim, Jang-Eok
    • Korean Journal of Environmental Agriculture
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    • v.15 no.4
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    • pp.434-441
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    • 1996
  • This study was conducted to clarify the degradation pattern, safety evaluation and penetration ratio of benomyl and bitertanol to apple used as postharvest fungicides during CA(controlled atmosphere) and cold storage. In CA storage, the degradation of benomyl and bitertanol in stored apple was slow at the early stage, while that in cold storage was, on the contrary, faster at the early stage. The initial concentrations of benomyl and bitertanol in apples applied at the standard application concentrations were 2.24 and 1.54mg/kg, respectively, and their residual amounts were below the maximum residue limits, 1mg/kg at 135 and 96 days in CA storage, 115 and 70 days in cold storage, respectively. The half-lives of benomyl and bitertanol in stored apples were 124 and 130 days in CA storage, 101 and 111 days in the cold storage, respectively, indicating that the degradation was faster in cold storage than in CA storage. The residual amount of benomyl and bitertanol in stored apples was retained more in peels than in pulps of apples.

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Geochemical characteristics of sediment, pore water, and headspace gas in the Ulleung Basin (울릉분지 퇴적물, 공극수 및 공기층 가스의 지화학적 특징)

  • Kim, Ji-Hoon;Park, Myong-Ho;Ryu, Byong-Jae;Lee, Young-Joo;Jin, Young-Keun
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.06a
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    • pp.373-376
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    • 2006
  • 본 연구의 목적은 동해 울릉분지의 제4기 후기 퇴적물 내의 유기물, 공극수와 메탄의 특징 및 상호작용을 규명하는데 있다. 연구지역에서 채취한 코어퇴적물을 원소 분석한 결과 C/N 및 C/S 비(wt. %)는 퇴적물 내 유기물이 주로 해양조류 기원을 가지고, 일반적인 해양 또는 정체 환경에서 퇴적되어Tdam을 지시한다. 그러나 Rock-Eval 열분석 결과는 유기물 기원이 육상식물(Type III)이고, 열적 성숙단계가 미성숙단계임을 보여준다. 이러한 원소분석과 열분석간의 상반된 결과는 유기물이 침강하는 동안 또는 퇴적 후 이루어진 강한 산화작용에 기인한 것으로 추정된다. 퇴적물 내 공극수의 황산염 농도가 퇴적물의 심도가 증가할수록 감소하며, 감소하는 경향은 크게 두 가지 (적선성, concave down)로 나누어진다. 이는 모든 코어에서 황산엽 환원작용이 일어나고 있음을 지시한다. 또한 직선선의 황산엽농도 구배는 무산소 메탄 산화작용(AMO)의 전형적인 특징이다. 황산염 농도의 수직적 구배를 이용하여 SMI(sulfate-methane interface) 심도를 계산하면, 남부울릉분지의 코어 (03GHP-01, 03GHP-02; <3.5mbsf)가 북부울릉분지 코어(01GHP-05, 01GHP-07, 03GHP-03, 03GHP-04, 03GHP-05; > 6mbsf)보다 낮은 값을 갖는다. 위와 같은 SMI 심도차는 메탄의 상부 분산량과 밀접한 관련있는 것으로 추정된다. 메탄가스의 탄소 안정동위원소 $({\delta}^{13}C)$ 분석값들은 -83.5%o에서 -69.5%o의 범위를 가지고 있고, 이산화탄소 환원작용($CO)_2$ reduction)에 의한 생물 (biogenic) 기원임을 지시한다.

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The Formative Processes and Ages of Paleo-coastal Sediments in Dangjeong-ri, Seocheon-gun in the Western Coast, South Korea (II): Complementation of Chronological Data and a Developmental Model of Coastal Geomorphology over the Past 200,000 Years (서해안 서천군 당정리 일대에 분포하는 육상 고해안 퇴적물의 형성 과정과 형성 시기(II): 추가 연대 자료 및 제4기 후기 연안 지형 발달 모델)

  • Shin, Jae-Ryul;Hong, Yeong-Min;Ryu, Hui-Gyeong;Hong, Seongchan
    • Journal of The Geomorphological Association of Korea
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    • v.28 no.3
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    • pp.51-61
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    • 2021
  • Following the previous study, we report a complementary dating data on the silty layers deposited in paleo-tidal conditions of the study area, Dangjeong-ri, Seocheon-gun and suggest coastal terrain development processes over the past 200,000 years. Based on the dating results, the silty layers distributed up to 25 m above mean sea level were deposited between 171 and 183 ka, and the gravel layer deposited in a fluvial environment of a paleo-Dangjeong stream was found to have formed between 78 and 83 ka. Considering relative altitudes of distribution, an uplift rate of the study area in the western coast is judged to be relatively 0.5~0.7 low to that of Pohang area in the eastern coast. Compared to Busan and Sacheon areas in the southern coast, it is assumed that an uplift rate of the study area shows a similar level with those during the late Quaternary.

Quality Characteristics of Kimchi according to Garlic Content during Fermentation (마늘 첨가량에 따른 김치의 발효 중 품질 특성 변화)

  • Choi, Yun-Jeong;Hwang, Ye-Seul;Hong, Sung Wook;Lee, Mi-Ai
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.11
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    • pp.1638-1648
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    • 2016
  • This study was conducted to analyze the quality characteristics of kimchi in terms of garlic content (0~4.5%). Kimchi was made at $4^{\circ}C$ for 8 weeks, and pH, acidity, organic acid content, free sugar content, microbial counts, flavor pattern, and sensory characteristics were measured. The results show that kimchi containing garlic had a higher pH and lower acidity during fermentation than control kimchi without garlic. Principal component analysis enabled differentiation of the flavor pattern of kimchi according to fermentation period and garlic content. Addition of garlic to kimchi significantly decreased the numbers of total bacteria and lactic acid bacteria for 2 weeks after production. The numbers of total bacteria and lactic acid bacteria increased rapidly up to 2 weeks during fermentation and thereafter decreased gradually. Coliform counts were higher in the control than in kimchi containing garlic, whereas there was no detection after 4 weeks. Yeast and mold counts decreased significantly with increasing garlic content during the initial fermentation stage. Counts could not be detected in kimchi containing garlic. After 4 weeks, counts could not be detected in kimchi without garlic. Among kimchi with different garlic contents, fermentation was slower in kimchi with high garlic content; scores for off-odor and off-note taste were lower as well.