• 제목/요약/키워드: 제한단편

검색결과 97건 처리시간 0.02초

Studied on Amplificative Efficiency of PCR of Predigested template DNA and GC Contents for RAPD Analysis in the Silkworm, Bombyx mori (누에의 RAPD 분석을 위한 primer의 GC 함량과 사전 제한효소 처리한 주형 DNA의 PCR 증폭효율에 관한 연구)

  • 이진성;황재삼;이상몽;황석조;강현아;성승현;서동상
    • Korean journal of applied entomology
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    • 제35권1호
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    • pp.58-65
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    • 1996
  • This experiment was carried out to evaluate the effect of sublethal doses of BPMC, etofenprox, and buprofezin on N. lugens. and its predator C. lividipennis. Buprofezin was found to be the most toxic to N. lugens and the most safe to C. lividipennis among the three insecticides, based on LD50 values. Selective toxicity index calculated by dividing LDSo value of C. lividipennis by that of N. lugens indicated that buprofezin was very safe to C. lividipennis, showing selective toxicity of 2703.3. Longevity and fecundity of N. lugens treated with LDIU and LDm of buprofezin and BPMC were not significantly different with those of untreated brown planthoppers. However, egg hatchability' of N. lugens was greatly reduced when treated with LDm of buprofezin, having the highest inhibition rate of 17.7%. Hatchability of eggs from insects treated with BPMC was similar to that of control. The oviposited peak of treated hoppers appeared late as compared to the untreated which showed the peak at early part of the ovipositional period. The longevity and fecundity of C. lividipennis treated with BPMC were significantly reduced as compared with the untreated. Etofenprox also induced fecundity reduction when treated with LDlo, and LDm. However, C. lividipennis treated with sublethal doses of buprofezin showed no redution in logevity and fecundity. From these results, it may be said that buprofezin can be used to control brown planthopper without disrupting of C. lividipennis population in the rice field.

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Studies on Nitrogen-Fixing Microorganisms in Rice Rhizosphere (벼 뿌리 부근에 서식하는 질소고정미생물에 관한 연구)

  • 정건섭;민태익;변유량;유주현
    • Microbiology and Biotechnology Letters
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    • 제13권3호
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    • pp.251-255
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    • 1985
  • Nitrogen-fixing bacteria were isolated from the rice rhizosphere of various paddy fields in our country. The screening of 235 isolates for nitrogen-fixing ability resulted in the isolation of Enterobacter agglomerans NFB264 and three Klebsiella pneumoniae NFB 3, NFB 320. Plasmids of various molecular weight from 1.7 to more than 84 Mal. were detected by agarose gel electrophoresis in three out of four isolates. But, these plasmids had not any nitrogen-fixing genes. Hybridization experiments using Klebsiella pneumoniae M5al nitrogen-fixing genes, nif Q-K and nif DH, as probes revealed the presence of homologous sequences in the chromosomal DNA of all isolates. However the restriction patterns of nif genes of the isolates by various restriction endonucleases were different to those of Klebsiella pneumoniae M5al.

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Isolation and Identification of the Bacteriophage P4 ost2, Suppressing sir Mutations of Bacteriophage P2 (박테리오파아지 P2의 sir 변이를 억제하는 새로운 박테리오파아지 P4 유도체인 P4 ost2의 분리와 동정)

  • Kim, Gyeong-Jin
    • Korean Journal of Microbiology
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    • 제39권4호
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    • pp.277-282
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    • 2003
  • Bacteriophage P4 ost2 which is the P4 mutant suppressing sir mutations of Bacteriophage P2, was isolated as a plaque-former by plating P4 ash8 sid 71 kmr intS on the lawn of P2 sir3 lysogen. P4 ost2 turned out to be the P4 mutant suppressing sir mutations of P2 in one-step growth experiments.

An Evaluation Methodology of Software Industries and Its Application (소프트웨어 사업자 평가 시스템 개발 및 활용에 관한 연구)

  • Leem Choon Seong;Seo Hyung Sik;Yoon Yong Ki;Hwang Hyun Jeong;Kim Jin Young;Heo Nam Hoon;Lee Sang Hoon
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 대한산업공학회/한국경영과학회 2002년도 춘계공동학술대회
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    • pp.50-57
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    • 2002
  • 소프트웨어 자제나 소프트웨어 관련 기업의 평가를 위한 기존의 평가 시스템들은 소프트웨어 조직의 다양성과 측정 요소들의 정성적인 특성 등으로 측정이 어려웠고 이로 인해 조직의 프로세스, 기업환경이나 제품 기능성과 같은 제품위주의 단편적인 측면만을 고려한 평가 시스템들이 주를 이루어왔다. 따라서 잠재적 고객의 소프트웨어 구매나 제휴 업체 선정 등에 있어 일부 제한적인 정보만을 이용할 수 있었고, 이로 인해 소프트웨어나 소프트웨어 업체의 결정에 있어서 합리적이며 적절하고 빠른 의사결정을 하는 데 한계가 있었다. 본 연구에서는 기존의 평가 시스템들이 안고 있는 한계를 극복하여, 기업이 가지고 있는 역량에 대한 측면과 기업이 제공하는 고객 서비스 그리고 제품에 대한 고객 만족도 측면을 동시에 고려할 수 있는 평가 시스템(SISW)을 개발하였으며. 이를 국내 유수한 소프트웨어 기업을 대상으로 적용하여 그 결과를 분석, 제시하였다.

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Didactical Contemplation on the Development Figure (전개도에 관한 교수학적 고찰)

  • Chung, Young Woo;Kim, Boo Yoon
    • School Mathematics
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    • 제16권2호
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    • pp.285-301
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    • 2014
  • Because a development figure is treated restrictively in elementary school, not only there are brought out the concept image, but also the definition of the development figure in textbook in not unique. Furthermore, since the comparison and analysis of 'definition element' between the materials in textbook are nor carried out, its educational value as well as the teaching for concepts and objectives does not give rise to public discussion. In this note, we will investigate the definition and teaching of the development figure in Korea and Japan. And through three viewpoints of its definition and related debate, we will consider the fundamental understanding and objectiveness of a development figure, and suggest its mathematical application. This study will promote teachers's critical and didactical insight for the didactical transposition.

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Genetic Diversity and Spatial Structure in Populations of Abelia tyaihyoni (줄댕강나무 (Abelia tyaihyoni) 집단의 유전다양성 및 공간구조)

  • Jeong, Ji-Hee;Kim, Kyu-Sick;Lee, Cheul-Ho;Kim, Zin-Suh
    • Journal of Korean Society of Forest Science
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    • 제96권6호
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    • pp.667-675
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    • 2007
  • The genetic diversity and the spatial structure in two populations of Abelia tyaihyoni in Yeongwol region were studied by employing I-SSR markers. In spite of the limited distribution and small population sizes of Abelia tyaihyoni, the amount of genetic diversity estimated at the individual level was comparable to other shrub species (S.I.=0.336, h=0.217). Genetic diversity at the genet level was very similar to that at individual level. (S.l.=0.339, h=0.219). About 18.7 percent of total variation was allocated between two populations, which was slightly higher or similar level as compared with other shrub species. Genotypic diversity estimated by the ratio of the number of genets ($N_G$) over the total number of individuals (N) and a modified Simpson's index ($D_G$) were also higher than those of other shrubs. The maximum diameter of a genet did not exceed 5.5 m. The high level of gene and genotypic diversity, and the relatively limited maximum diameter of a genet suggested that the clonal propagation is not the most dominant factor in determining the population structure of Abelia tyaihyoni. Spatial autocorrelation analysis revealed significant spatial genetic structure within 12 m and 18 m distances in two populations A and B, respectively. Autocorrelations among individuals at the both individual and genet levels in each population didn't show any considerable differences. As a sampling strategy for ex-situ conservation of populations showing continuous distribution, a minimum distance of 18 m between individuals was recommended. For the populations with many segments, it was considered very crucial to sample materials from as many segments as possible.

Analysis on Energy Consumption Required for Building DTLS Session Between Lightweight Devices in Internet of Things (사물인터넷에서 경량화 장치 간 DTLS 세션 설정 시 에너지 소비량 분석)

  • Kwon, Hyeokjin;Kang, Namhi
    • The Journal of Korean Institute of Communications and Information Sciences
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    • 제40권8호
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    • pp.1588-1596
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    • 2015
  • In the Internet of Things (IoT), resource-constrained devices such as sensors are capable of communicating and exchanging data over the Internet. The IETF standard group has specified an application protocol CoAP, which uses UDP as a transport protocol, allows such a lightweight device to transmit data. Also, the IETF recommended the DTLS binding for securing CoAP. However, additional features should be added to the DTLS protocol to resolve several problems such as packet loss, reordering, fragmentation and replay attack. Consequently, performance of DTLS is worse than TLS. It is highly required for lightweight devices powered by small battery to design and implement a security protocol in an energy efficient manner. This paper thus discusses about DTLS performance in the perspective of energy consumption. To analyze the performance, we implemented IEEE 802.15.4 based test network consisting of constrained sensor devices in the Cooja simulator. We measured energy consumptions required for each of DTLS client and server in the test network. This paper compares the energy consumption and amount of transmitted data of each flight of DTLS handshake, and the processing and receiving time. We present the analyzed results with regard to code size, cipher primitive and fragmentation as well.

Genetic Differentiation between Up- and Downstream Populations of Tribolodon hakonensis (Pieces: Cyprinidae) (삼척오십천 상.하류에 분포하는 황어, Tribolodon hakonensis (잉어과) 집단의 유전적 분화)

  • Lee, Sihn-Ae;Lee, Wan-Ok;Suk, Ho-Young
    • Korean Journal of Environment and Ecology
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    • 제26권4호
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    • pp.475-483
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    • 2012
  • Tribolodon hakonensis(Cypriniformes; Leuciscinae) is anadromous; they are born in freshwater, migrate back to the ocean, then return to their home stream for spawning from mid-March to early-June. Here, five microsatellites were used to assess the level of gene flow among T. hakonensis populations from the Samcheok-Oship Stream, South Korea. The frequencies of dominant alleles across several loci differed between down-and upstream populations divided by several weirs, and pairwise multilocus $F_{ST}$ estimate was significantly high(0.083). However, there were no signs of any loss of genetic variation in the upstream population. Assignment tests of individuals in admixture model(K=2) to a set of baseline samples showed fairly correct assignment to each cluster; all of upstream individuals sere included in the first cluster, while the majority of downstream individuals(65%) comprise the second cluster. These results indicate reduced gene flow between up- and downstream populations but allowing passive downstream drift. It is likely that man-made structures might at least partially be a factor for creating and consolidating the current distribution patterns of genetic variation among T. hakonensis populations in the Samcheok-Oship Stream. This information will assist governing agencies in making informed decisions regarding conservation of anadromous fishes in Korean drainage systems.

Molecular Cloning and Analysis of the Genes in the Vicinity of Streptomyces griseus Trypsin (SGT) Gene from Streptomyces griseus ATCC10137 (Streptomyces griseus ATCC10137에서 Trypsin 유전자 sprT의 주변 유전자군 분석)

  • Chi Won-Jae;Kim Mi-Soon;Kim Jong-Hee;Kang Dae-Kyung;Hong Soon-Kwang
    • Korean Journal of Microbiology
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    • 제41권4호
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    • pp.255-261
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    • 2005
  • A 6.7kb DNA fragment containing the sprT gene encoding Streptomyces griseus trypsin (SGT) was cloned from Streptomyces griseus ATCC 10137, and the complete nucleotide sequence was determined. Nucleotide sequence and deduced amino acid or the EcoRI-HindIII fragment revealed the presence or the six complete ORFs containing the sprT gene and one incomplete ORF, which were named ORF1, SGT, ORF2, ORF3, ORF4, ORF5, and ORF6, respectively. ORF1 has homology with the oxidoreductases from several organisms. ORF2 and ORF3 show similarity with unknown proteins and transcription regulator that belongs to the ArsR family, respectively. ORF4 and ORF5 show homology with the peptidoglycan bound protein with LPXTG motif from Listeria monocytogenes and the membrane protein with transmembrane helix from several organisms, respectively. The last ORF, ORF6, shows homology with the lipoprotein from Streptomyces avermitilis.

An Efficient Dissemination Protocol for Remote Update in 6LoWPAN Sensor Network (6LoWPAN상에서 원격 업데이트를 위한 효율적인 코드 전파 기법)

  • Kim, Il-Hyu;Cha, Jung-Woo;Kim, Chang-Hoon;Nam, In-Gil;Lee, Chae-Wook
    • Journal of the Institute of Convergence Signal Processing
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    • 제12권2호
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    • pp.133-138
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    • 2011
  • In IP-based wireless sensor networks (WSNs), it might be necessary to distribute application updates to the sensor nodes in order to fix bugs or add new functionality. However, physical access to nodes is in many cases extremely limited following deployment. Therefore, network reprogramming protocols have recently emerged as a way to distribute application updates without requiring physical access to sensor nodes. In order to solve the network reprogramming problem over the air interface, this thesis presents a new scheme for new update code propagation using fragmentation scheme and network coding. The proposed code propagation method roughly shows reduced performance improvement in terms of the number of data exchange compared with the previously proposed pipelining scheme. Further, It is shows enhanced reliability for update code propagation and reduced overhead in terms of the number of data exchange. As a result, we can efficiently perform the software update from the viewpoint of speed, energy, and network congestion when the proposed code propagation system is applied. In addition, the proposed system solves overhearing problems of network coding such as the loss of original messages and decoding error using the predefined message. Therefore, our system allows a software update system to exchange reliable data in wireless sensor networks.