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Molecular Cloning and Analysis of the Genes in the Vicinity of Streptomyces griseus Trypsin (SGT) Gene from Streptomyces griseus ATCC10137  

Chi Won-Jae (Department of Biological Science, Myongji University)
Kim Mi-Soon (Department of Biological Science, Myongji University)
Kim Jong-Hee (Department of Biological Science, Myongji University)
Kang Dae-Kyung (Resources Institute, Easy Bio System Inc.)
Hong Soon-Kwang (Department of Biological Science, Myongji University)
Publication Information
Korean Journal of Microbiology / v.41, no.4, 2005 , pp. 255-261 More about this Journal
Abstract
A 6.7kb DNA fragment containing the sprT gene encoding Streptomyces griseus trypsin (SGT) was cloned from Streptomyces griseus ATCC 10137, and the complete nucleotide sequence was determined. Nucleotide sequence and deduced amino acid or the EcoRI-HindIII fragment revealed the presence or the six complete ORFs containing the sprT gene and one incomplete ORF, which were named ORF1, SGT, ORF2, ORF3, ORF4, ORF5, and ORF6, respectively. ORF1 has homology with the oxidoreductases from several organisms. ORF2 and ORF3 show similarity with unknown proteins and transcription regulator that belongs to the ArsR family, respectively. ORF4 and ORF5 show homology with the peptidoglycan bound protein with LPXTG motif from Listeria monocytogenes and the membrane protein with transmembrane helix from several organisms, respectively. The last ORF, ORF6, shows homology with the lipoprotein from Streptomyces avermitilis.
Keywords
ArsR; oxidoreductase; lipoprotein; peptidoglycan bound protein; SGT; transmembrane protein;
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