• Parasites, Hosts and Diseases
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• v.30 no.4
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• pp.283-288
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• 1992

To observe the transmission patterns of karyotype of Pneumocystis carinii (Pc) by rat colonies, three strains of rats, Sprague-Dawlcy(SD), Wistar(W) and Fisher (F) from various animal vendors, were suppressed of their immunity by injection of methyl prednisolone. They were kept for 5 to 13 weeks in 3 different animal rooms, A, B, and C. The purified organisms were prepared in low melting point agarose gel by embedded Iysis method for pulsed field gel electrophoresis. Field inversion gel electrophoresis showed 2 patterns of the kart·otype of Pc. The rooms A and C contained SD rats from the source p, and also the room A was used for F and W rats. However, Pc from all of the SD and F rats in the room A showed same karyotypes, the pattern I. The SD rats from difFerent vendors, M and 5, were reared in the room B, and shared the same Pc karyotypes, the pattern II . The rats of W strain were from the vendor M, and immune-suppressed in the animal room A. Five weeks after the expe- riment, the Pc showed the karyotype pattern II but the pattern became mixed with the type I after 7 to 8 weeks. The Bindings revealed that the animals born and reared in the same animal quarter harbored Pc with same karyotypes. If the animals were kept under immune-suppression in the same room with heavily infected hosts, they could be infected by Pc from their neighbors. The present experimental findings suggest that Pc is transmitted among rats through the air.

• Korean Journal of Remote Sensing
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• v.29 no.5
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• pp.555-568
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• 2013

Atmospheric optical thickness during nighttime was estimated in this study using analysis on the images of the moon taken from commercial digital camera. Basically the Langely Regression method was applied to the observations of the moon for the cloudless and optically stable sky conditions. The spectral response functions for the red(R), green(G), and blue(B) channels were employed to derive effective wavelength centers of each channel for the observations of the moon, and the correspondent Rayleigh optical thickness were also calculated. Aerosol optical thickness (AOT) was calculated by subtracting Rayleigh optical thickness from the atmospheric optical thickness derived from the Langley regression method. As there are only handful of nighttime AOT observations, the AOT from the moon observations was compared with the AOT from sun-photometers and the MODIS satellite sensor, which was taken several hours before the moon observations of this study. As a result, the values of AOT from moon observations agree with those from sun-photometers and MODIS within 0.1 for the R, G, B channels of the digital camera. On the other hand, ${\AA}$ngstr$\ddot{o}$m Exponent seems to be subject to larger errors due to its sensitiveness to the spectral errors of AOT. Nevertheless, the results of this study indicate that the method reported in this study is promising as it can provide nighttime AOT relatively easily with a low cost instrument like digital camera. More observations and analyses are warranted to attain improved nighttime AOT observations in the future.

• 한국HCI학회:학술대회논문집
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• 2006.02a
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• pp.1031-1036
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• 2006

햅틱 분야는 디스플레이 되는 콘텐츠를 만질 수 있게 촉감을 제공함으로써 의학, 교육, 군사, 방송 분야 등에서 널리 연구되고 있다. 이미 의학 분야에서는 Reachin 사(社)의 복강경 수술 훈련 소프트웨어와 같이 실제 수술 할 때와 같은 힘을 느끼면서 수술 과정을 훈련할 수 있는 제품이 상용화 되어 있다. 그러나 햅틱 분야가 사용자에게 시청각 정보와 더불어 추가적인 촉감을 제공함으로써 보다 실감 있고 자연스러운 상호작용을 제공하는 장점을 가진 것에 비해 아직은 일반 사용자들에게 생소한 분야다. 그 이유 중 하나로 촉감 상호작용이 가능한 콘텐츠의 부재를 들 수 있다. 일반적으로 촉감 콘텐츠는 컴퓨터 그래픽스 모델로 이루어져 있어 일반 그래픽 모델러를 사용하여 콘텐츠를 생성하나 촉감과 관련된 정보는 콘텐츠를 생성하고 나서 파일에 수작업으로 넣어주거나 각각의 어플리케이션마다 직접 프로그램을 해주어야 한다. 이는 그래픽 모델링과 촉감 모델링이 동시에 진행되지 않기 때문에 발생하는 문제로 촉감 콘텐츠를 만드는데 시간이 많이 소요되고 촉감 정보를 추가하는 작업이 직관적이지 못하다. 그래픽 모델링의 경우 눈으로 보면서 콘텐츠를 손으로 조작할 수 있으나 촉감 모델링의 경우 손으로 촉감을 느끼면서 동시에 조작도 해야 하기 때문에 이에 따른 인터페이스가 필요하다. 본 논문에서는 촉감 상호작용이 가능한 촉감 콘텐츠를 직관적으로 생성하고 조작할 수 있게 하는 촉감 모델러를 기술한다. 촉감 모델러에서 사용자는 3 자유도 촉감 장치를 사용하여 3 차원의 콘텐츠를 실시간으로 만져보면서 생성, 조작할 수 있고 촉감 사용자 인터페이스를 통해서 콘텐츠의 표면 촉감 특성을 직관적으로 편집할 수 있다. 촉감 사용자 인터페이스는 마우스로 조작하는 기존의 2차원 그래픽 사용자 인터페이스와는 다르게 3 차원으로 구성되어 있고 촉감 장치로 조작할 수 있는 버튼, 라디오 버튼, 슬라이더, 조이스틱의 구성요소로 이루어져 있다. 사용자는 각각의 구성요소를 조작하여 콘텐츠의 표면 촉감 특성 값을 바꾸고 촉감 사용자 인터페이스의 한 부분을 만져 그 촉감을 실시간으로 느껴봄으로써 직관적으로 특성 값을 정할 수 있다. 또한, XML 기반의 파일 포맷을 제공함으로써 생성된 콘텐츠를 저장할 수 있고 저장된 콘텐츠를 불러오거나 다른 콘텐츠에 추가할 수 있다.

• Journal of Chest Surgery
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• v.29 no.8
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• pp.836-843
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• 1996

Histologic, and scanning electron microscopic observa ions were made of 12 biopsy specimens from polytetrdiluoroethylene (PTFE) grafts that had been Implanted as systemic-pulmonAry shunt for improving oxygenation of cyanotic congenital heart disease and harvested near the end of pulmonary artery side at the time of redo shunt or tonal correction between 1985 and 1992. The types of shunt operation are modified Blalock-Taussig shunt in 10 cases, Waterston-Cooley shunt in 1 And Potts shunt in 1 case. The 5 mm PTFE graft was used In 10, 4mm in 1 and 6mm in 1 case. The mean duration of implantalion was 0 $\pm$ 14.1 months(rl.on 12 months to 55 months). The plAtelets were aggregated between gaps'of Coretex surface and intimal thickening was noted about 10 to 20 months after implantation. Endothelial cells were found in the 40-months patent PTFE graft by light and scanning electron microscopy. In the specimen of poor flow or zero flow graft, severe intimal hyperplasia and thrombi which was made of platelets were noted . Based on this experience, we think that the patency will be maintained well if the connective tissue could be Hxed firmly over the Inner layer of the Goretex and the endothelial cell layer sllould form over the con- nective tissue and platelet aggregation should be prevented.

• Journal of Life Science
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• v.8 no.6
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• pp.627-637
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• 1998

In order to utilize tuna pyloric caeca among fish intestines wasted when treated raw fish in fish processing manufactory, a crude enzyme with high proteolytic activity was extracted and its optimum condition were investigated. An immobilized enzymes also were prepared by adsorption method to enhance thermostability of the crude proteinase. The yield of the crude proteinase was approximately 2.7% on dry basis. The proteolytic activity for casein was 0.54 U/mg protein, for BTEE 1.10 U/mg protein, and for BAEE 2.69 U/mg protein. It was almost similar to that of the commercial trypsin purified. Optimum hydrolysis activity of the crude proteinase was about 80%, as the degree of hydrolysis for casein, at pH 10.0 and 45$^{\circ}C$ for 12 hrs. Also, when the crude proteinase was immobilized on DEAE-Cellulose and chitin, the residual activities remained after 7 days of pre-incubation time were maintained about 90% or more and their thermostabilities were enhanced by about 50%, compared with the native enzyme.

• Microbiology and Biotechnology Letters
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• v.20 no.6
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• pp.625-629
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• 1992

This work was carried out to study physiological characteristics of Rhodosporidium toru[oides cells having two different mating types. The mating type A produces internal. cell wall-bound, and external invertases while type a produces only two invertases except external invertase. Comparing their characteristics after partial purification of internal invertases from both mating type cells, invertase from type a has decreased 15% of invertase activity only by $Mn^{2+}$ I while invertase from type A has been increased 11% of invertase activity by $Zn^{2+}$ and decreased 15% of invertase activity by $Mn^{2+}$ On the effect of enzyme inhibitor, invertase of type a was inhibited from 12% to 57% by 2-mercaptoethanol, sodium dodecyl sulfate, phenol. but invertase of type A was slightly inhibited only by phenol. The thermal stability of both invertases has showed steep inactivation at above $80^{\circ}C$ and their optimal temperatures were similar at $60^{\circ}C$ . Invertase from type A showed stability only on condition of acid from pH 3 to 6 and its opimal pH was 5.0, while invertase from type a showed stability at the wide range of pH 3-10 and its optimal pH was 4.0. And the $K_m$ values of invertases from type A and type a were $2.5{\times}10^3$M and$3.4{\times}10^3$M, respectively.

• Journal of Korean Tunnelling and Underground Space Association
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• v.20 no.3
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• pp.640-655
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• 2018

Recently, the construction of the urban area has been rapidly increasing, and the excavation work of the ground has been frequently performed at the upper part of the existing underground structures. Especially, when the structure is constructed after the excavation of the ground, the loading and unloading process in the ground under the excavation basement can affect the existing underground structures. Therefore, in order to maintain the stability of the existing underground structure due to the excavation of the ground, it is necessary to accurately grasp the influence of the excavation and the structure load in the adjoining part. In this study, the effect of the excavation of the ground and the new structure load on the existing tunnel was experimentally implemented and the influence of the adjacent construction on the existing tunnel was investigated. For this purpose a large testing model with 1/5 scale of the actual size was manufactured. The influence of ground excavation, width of the load due to new structure, and distance between centers of tunnel and of excavation on the existing tunnel was investigated. In this study, it was confirmed that the influence on the existing tunnel gets larger, as the excavation depth get deeper. At the same distance, it was confirmed that the tunnel displacement increased up to three times according to the increase of the building load width. That is, the load width influences the existing tunnel larger than the excavation depth. As the impact of the distance between centers of tunnel and of excavation, it was confirmed that tunnel crown displacement decreased by 48%. The result showed that a tunnel is located in the range of 1D (D: tunnel diameter) from the center of excavation, the effect of excavation is the largest.

• Microbiology and Biotechnology Letters
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• v.10 no.4
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• pp.267-273
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• 1982

Among 12 kinds of ginsenosides in ginseng saponin, ginsenoside-Rb$_1$was contained the most abundantly. But ginsenoside-Rd which is similar to ginsenoside-Rb$_1$in structure, was known to be superior to ginsenoside-Rb$_1$pharmaceutically. In order to convert ginsenoside-Rb$_1$into ginsenoside-Rd by microbial enzyme treatment, a Rhizopus sp. was selected among various strais of molds found in rotten ginseng roots. Enzyme was prepared from the extract of wheat bran koji culture by ammonium sulfate precipitation (1.0 sat'd) and succeeding ammonium sulfate fractionation method (0.6-0.9 sat'd). For the purpose of use as substrate, saponins were purified by the several purification steps from alcohol extract of red ginseng roots. We obtained the total saponin which was composed of 36.5% of ginsenoside Rb$_1$, 12.2% of ginsenoside-Rd and other ginsenosides. For increase of ginsenoside-Rb$_1$ component ratio, we also obtained further purified ginsenoside-Rb group saponin containing 54.5% of ginsenoside-Rb$_1$, 1.1% of ginsenoside- Rd and other ginsenosides from purified the total saponin. In the enzymatic reaction system including the total saponin or the ginsenoside-Rb group saponin, we confirmed the specific conversion of ginsenoside-Rb$_1$to ginsenoside-Rd proportionally and no change of any other ginsenoside patterns by thin layer chromatography and high performance liquid chromatography.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.2
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• pp.153-157
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• 2000

Hydrolysate which inhibit the Angiotensin I-converting enzyme (ACE) was prepared from mackerel muscle by pretense. The ACE inhibitory activity of mackerel muscle hvdrolysate (MMH) was $967 {\mu}g of IC_(50)$. ACE inhibitory peptides were isolated by ultrafiltration, gel permeation column chromatography (GPC), reversed phase column chromatography(RPC), reversed phasehigh performance liquid chromatography (RP-HPLC), and gel permeation high performance liquid chromatography (GP-HPLC) from the MMH. The amino acid sequence of the ACE inhibitory peptides was Tyr-Val-Ala. The $IC_(50)$ of this peptide for ACE from rabbit lung was $1.4 {\mu}M$.

• The Journal of the Acoustical Society of Korea
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• v.18 no.5
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• pp.68-77
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• 1999

The paper looked into the effects of the spectral properties (waveform) of the high intensity focused ultrasound on suppression of the ultrasonic cavitation. Three different types of ultrasound were considered in the study, which were sinusoidal (1 MHz, 5 MPa), frequency modulated (from 1 MHz to 6 MHz for 10 ㎲, 5 MPa), asymmetrically shocked (fundamental frequency 1 MHz, peak positive pressure 12 MPa, peak negative pressure -4 MPa). The temporal response of an air bubble in water initially 1 ㎛ in radius to each type of the ultrasound was predicted using Gilmore bubble dynamic model and Church's rectified gas diffusion equation. It was shown that the radially pulsating amplitude of the bubble was greatly reduced for the frequency modulated wave and was little decreased for the shock wave, compared to the case that the bubble was exposed to the sinusoidal wave. It is interesting that the bubble response to the frequency modulated wave remains similar when the frequency component of the modulated ultrasound is beyond the bubble resonant frequency 3 MHz. This implies that, although the ultrasound is modulated up to 3MHz rather than up to the present 6 MHz, it is likely to produce similar cavitation suppression effects. In practice, it means that a typical narrow band ultrasonic transducer can be taken to generate an appropriate frequency modulated ultrasound to reduce cavitation activity. The present study indicates that ultrasonic cavitation may be suppressed to some extent by a proper spectral modification of high intensity ultrasound.