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Effects of Protox Herbicide Tolerance Rice Cultivation on Microbial Community in Paddy Soil (Protox 제초제저항성 벼 재배가 토양미생물 군집에 미치는 영향)

  • Oh, Sung-Dug;Ahn, Byung-Ohg;Kim, Min-Kyeong;Sohn, Soo-In;Ryu, Tae-Hun;Cho, Hyun-Suk;Kim, Chang-Gi;Back, Kyoung-Whan;Lee, Kijong
    • Korean Journal of Environmental Agriculture
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    • v.32 no.2
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    • pp.95-101
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    • 2013
  • BACKGROUND: Rice (Oryza sativa) is the most important staple food of over half the world's population. This study was conducted to evaluate the possible impact of transgenic rice cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere of GM and non-GM rice cultivation soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with GM and non-GM rice were similar to each other, and there was no significant difference between GM and non-GM rice. Dominant bacterial phyla in the rhizosphere soils cultivated with GM and non-GM rice were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in GM and non-GM rice cultivated soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed similar patterns, but didn't show significant difference to each other. DNAs were isolated from soils cultivating GM and non-GM rice and analyzed for persistence of inserted gene in the soil by using PCR. The PCR analysis revealed that there were no amplified protox gene in soil DNA. CONCLUSION(S): These data suggest that transgenic rice does not have a significant impact on soil microbial communities, although continued research may be necessary.

Effect of Insecticide and Fungicide on Phytotoxicity of Herbicide in Rice (수도(水稻)에 있어서 제초제(除草劑)의 약해(藥害)에 미치는 살충(殺蟲), 살균제(殺菌劑)의 영향(影響))

  • Chun, J.C.;Hwang, I.T.;Han, M.S.;Jang, B.C.
    • Korean Journal of Weed Science
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    • v.6 no.1
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    • pp.67-75
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    • 1986
  • This study was undertaken to investigate the interrelation between herbicide and insecticide and fungicide on rice (Oryza saliva L.) at various growth stages. Carbamate insecticide BPMC (2-sec-butylphenylmethyl-carbamate) severely inhibited germination of rice when applied alone and in combination with herbicides tested. No germination inhibition was obtained with thio- and dithiocarbamate pesticides. Post-germination growth of rice was severely inhibited by the treatments which were combined with BPMC irrespective of herbicides studied. Phytotoxicity of pendimethalin (3,4-dimethyl-2,6-dinitro-N-1-ethyl propylamine) was reduced by antagonistic effect of organophosphorus compounds. When herbicides were applied with either insecticide or fungicide, post-germination growth of rice was more greatly affected by the concentration of herbicides than that of insecticides or fungicides. Most of herbicide-insecticide or herbicide-fungicide treatments did not cause great phytotoxicity on rice when applied 5 days after transplanting. Foliage activity of phenoxy herbicide 2,4-D (2,4-dichlorophenoxy acetic acid) and MCPA [(4-chloro-o-tolyl) oxy acetic acid] increased with addition of carbamate and urea pesticides.

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Classification of Herbicidal Spectrum by Two-Dimensional Ordination Analysis in Soybean Field (대두포장(大豆圃場)에서의 Two-dimensional Ordination 분석법(分析法)에 의한 제초제(除草劑) 살초(殺草) Spectrum 분류(分類))

  • Kang, B.H.;Kim, H.S.;Kim, T.W.;Yong, P.S.;Ahn, C.W.
    • Korean Journal of Weed Science
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    • v.10 no.3
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    • pp.192-196
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    • 1990
  • Eleven herbicides were treated in soybean fields of Dukso and Yeoncheon in Gyeonggi province. These herbicides were classified by two-dimensional ordination analysis based on important values of the weed flora which were obtained after application of herbicides. Weed community types were E. crus-galli(56%)-D. adscendens (14%) -C. album (10%)-P. oleracea (8%) and P. oleracea (58%)-E. crus-galli (29%) -A. mangostanus (5%) -D. adscendens (3%), respectively. From soybean field at Dukso, 11 weed community types or 11 herbicide groups were obtained. And at Yeoncheon, 9 weed community types or 9 herbicide groups were classified. At treated blocks with clomazone and bentazon, C. amuricus and E. crus-galli dominated respectively. And at treated blocks with quizalofop, haloxifop and alloxydium, P. oleracea dominated remarkably. The herbicides classification by the two-dimensional ordination analysis could be used more effectively to selecting herbicides for reciprocal and systematic weed control than by similarity analysis.

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Effects of Several Herbicides on Control of Creeping Bentgrass in the Kentucky Bluegrass and Its Recovery (켄터키블루그래스 포장에 침입한 크리핑벤트그래스의 제초제를 이용한 방제와 회복)

  • Kim, Young-Sun;Jo, Kap-Jun;Lee, Hyo-Sun;Kang, Young-Nam;Lim, Hye-Jung;Lee, Kyu-Seong
    • Weed & Turfgrass Science
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    • v.2 no.4
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    • pp.395-401
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    • 2013
  • This study was conducted to evaluate the selective control of creeping bentgrass invaded in Kentucky bluegrass by applying several herbicides and recovery of Kentucky bluegrass by sand injection seeding method. Selective herbicides such as mecoprop, triclopyr-TEA, imazaquin, bentazone and penosulam pyrazosulfuro-ethyl and non-selective herbicides such as glyphosate, paraquat dichloride and glyphsate ammonium oxyflorfen were used. Selective suppression of creeping bentgrass in Kentucky bluegrass was evaluated by turf color, chlorophyll and visual control indexes. Control of creeping bentgrass was most effective with the double dose application of triclopyr-TEA (2 Tri-T) in the selective herbicides and the 1 / 5 dose application of glyphosate ammonium oxyflorfen (1 / 5 GAO) in the non-selective herbicides. Visual control indexes by 2 Tri-T in selective herbicides and 1 / 5 GAOin non-selective herbicides were investigated 6.0 and 7.4, respectively. Treated sites were covered completely in 50 days after seeding Kentucky bluegrass by sand injection method.

The Study of Chemical Weed Control Systems in Orchard (과수원(果樹園)의 잡초방제(雜草防除) 체계확립(體系確立)에 관한 연구(硏究))

  • Kim, K.Y.;Kim, J.K.;Cho, M.D.;Kim, S.B.
    • Korean Journal of Weed Science
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    • v.4 no.2
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    • pp.211-218
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    • 1984
  • In order to establish the chemical weed control systems in orchard, field experiments were conducted to evaluate the effects of spray methods and mixing of herbicides. The mixtured dosage of glyphosate 100cc/10a and 2,4-D 75㏄/10a showed higher control effects than mono-treatment of glyphosate and paraquat on Artemisia vulgaris var. indica and Polygonum hydropiper L. And oxyfluorfen was more effective in reducing the cover rate and dry weight of weeds than simazine and alachlor on 75th day after treatment. In the mixing experiment of land and foliar appling herbicides. The effect of glyphosate on May 17 followed by oxyfluorfen on June 12 showed the best control effect.

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Weed Control with Herbicides in Protected Semi - irrigated Rice Seedbed (보온절충(保溫折衷) 못자리에 있어서 제초제(除草劑)에 의한 잡초방제(雜草防除)에 관한 연구(硏究))

  • Ryang, Hwan-Seung;Yim, Ju-Hyuck
    • Korean Journal of Weed Science
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    • v.4 no.2
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    • pp.169-178
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    • 1984
  • This experiment was conducted to find out selective herbicides which are safe to the rice seedlings and to provide effective weed control method in protected semi-irrigated rice seedbed. There was no crop injury in rice with benzophenap [2-(4-2, 4-dichloro-3-methylbenzoyl)-1, 3-dimethyl-pyrazol-5-yl-oxy)-4'-methyl acetophenone] (240g), pyrazoxyfene [1,3-dimethyl-4-(2,4-dichlorobenzoyl)-Sphenacyloxy pyrazole] (200g), chlormethoxynil [2,4-dichlorophenyl-4-nitro-3-methoxyphenyl ether] (180g), dimepiperate [S-(1-methyl-1-phenethyl)-piperidine-l-carbathioate] (210g), dimepiperate + probenazol [3-allyloxy-1,2-benzisothiazol-1,1-dioxide] (210 + 120g) mixture and dimepiperate + probenazol + molinate [S-ethyl-N,N-hexamethylene-thiol-carbamate] (120 + 120 + 120g) mixture at times of application studied. Butachlor [2-chloro-2',6'-diethyl-N-buthoxymethyl acetanilide] + pyrazolate [4-(2,4-dichlorbenzoyl)-1,3dimethyl-pyrazol-5-yl-p-toluene-sulfonate] (70 + 120g) mixture and butachlor + chlormethoxynil (60 + 120g) mixture caused root length to shorten and root viability to decrease. However, the crop injury was recovered at 25 days after seeding. Benzophenap, pyraxoxyfene and butachlor + pyrazolate mixture were effective for weed control when applied at 2 days before seeding. Chlormethoxynil and butachlor + chlormethoxynil mixture was very effective for controlling annual weed. Dimepiperate, dimepiperate + probenazol mixture and dimepiperate + probenazol + molinate mixture did not control most weeds except for Echinochloa crus-galli (L.) Beauv.

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Alternative Herbicides to Control Herbicide-Resistant and Troublesome Weeds in Paddy Fields (제초제 저항성 및 난방제 잡초 방제를 위한 제초제 선발)

  • Park, Tae-Seon;Lee, In-Yong;Seong, Ki-Yeong;Cho, Hyun-Suk;Kim, Mi-Hyang;Yang, Woon-Ho;Seo, Myung-Chul;Kang, Hang-Won
    • Weed & Turfgrass Science
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    • v.2 no.3
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    • pp.248-253
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    • 2013
  • This study was conducted to select alternative herbicides to control herbicide-resistant and - troublesome weeds in rice fields, Korea. The resistant Echinochloa oryzicola to ACCase inhibitor was tested by herbicides registered to control Echinochloa oryzicola. And the resistant and troublesome weeds to sulfonylurea (SU) herbicides, ALS inhibitors, were tested by widely using herbicides to control the resistant weeds in Korea. The oxadiazon, pyrazolate, pretilachlor and benzobicyclone+thiobencarb effectively controlled resistant Echinochloa oryzicola to ACCase and ALS inhibitors at 0.5 leaf stage of the, Herbicides containing mefenacet or fentrazamide controlled effectively by the 2 leaf stage. In controlling other SU resistant weeds including Sagittaria trifolia and Ludwigia prostrate, benzobicyclon and mesotrione performed well all tested weeds, and carfentrazone and pytazolate were effective to broadleaf weeds. Sagittaria trifolia and Ludwigia prostrate, which were suspected to be resistance to SU herbicides, were well controlled by carfentrazone and pytazolate.

Occurrence Trends of SU-Herbicide Resistant Weeds in Paddy Fields in Korea (설포닐우레아계 제초제 저항성 논잡초 발생동향)

  • Lee, In-Yong;Won, Tae-Jin;Seo, Young-Ho;Kim, Eun-Jung;Yun, Yeo-Tack;Cho, Seng-Hyun;Kwon, Oh-Do;Kim, Sang-Kuk;Chung, Wan-Gyu;Park, Tae-Seon;Kim, Chang-Seok;Lee, Jeongran;Moon, Byung-Chul;Park, Jae-Eup
    • Weed & Turfgrass Science
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    • v.2 no.3
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    • pp.318-321
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    • 2013
  • National Academy of Agricultural Science and eight province Agricultural Research & Extension Services investigated the occurring area of herbicide resistant weeds in paddy field of Korea. In order to estimate the occurring areas of herbicide resistant weeds, we collected paddy soils randomly from 3,200 sites in 2011 and 2012, and treated 30 kg $ha^{-1}$ of pyrazosulfuron-ethyl+pyriminobac-methyl GR to the paddy soil. 176,870 ha, approximately 22.1% of cultivated area excluding organic and eco-friendly cultivated area, was estimated to be infested by SUherbicide resistant paddy field weeds. Several species of resistant weeds were occurred at Jeonranam-do with 44.3%, followed by 30.5% at Chungchungnam-do, 27.8% at Chungchungbuk-do, and 24.5% at Jeonrabuk-do, respectively. Monochoria vaginalis showed the highest distribution with 57,018 ha, 32.2% followed by Scirpus juncoides, and Lindernia procumbens, respectively.

Selection of Appropriate Herbicides for Establishment of Weed Control System in Adzukibean and Mungbean (팥과 녹두 잡초방제체계(雜草防除體系) 수립(樹立)을 위한 제초제(除草劑) 병발(迸拔)에 관한 연구)

  • Hong, E.H.;Lee, Y.H.;Kim, S.D.;Hwang, Y.H.;Moon, Y.H.
    • Korean Journal of Weed Science
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    • v.3 no.2
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    • pp.199-207
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    • 1983
  • To select appropriate herbicides for adzukibean and mungbean, a series of experiments was carried out in both field and pot from 1980 to 1983. Tolerance to the herbicides tested was highest in soybeans and followed by mungbean and adzukibean in the order. Pre-emergence herbicides showed relatively low phytotoxicity were chlorambem, linuron, and metribuzin for adzukibean and alachlor and butachlor for mungbean. Post-emergence herbicides showed no external phytotoxicity for adzukibean and mungbean were alloxydim, Dowco 453, fluazifop, etc.. For mungbean, terbutryn (1.5 kg, a.i./ha) which is pre-emergence herbicide showed the best weeding control efficacy but some possible phytotoxicity. Among post-emergence herbicides, acifluorfen (300 g, a.i./ha) showed the best weeding efficacy with no yield reduction though some phytotoxicity which recovered within 20 days. Compared to single herbicide application, the mixture or systemic treatments of herbicides showed much higher weeding control efficacy and seed yields: the systemic treatments of linuron (500 g, a.i./ha) / Dowco 453 (180 g, a.i./ha) or linuron (500 g, a.i./ha) / fluazufop (260 g, a.i./ha) for adzukibean and the mixture treatment of alachlor (1.31 kg, a.i./ha) / acifluorfen (150 g, a.i./ha) for mungbean, respectively.

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Effective Weed Control in Paddy Field Simultaneously Dominated by Herbicide-Resistant Weeds, Echinochloa oryzoicola, Monochoria vaginalis and Scirpus juncoides (제초제 저항성 잡초 강피, 물달개비, 올챙이고랭이 동시 우점한 논에서 효과적인 제초관리)

  • Park, Tae Seon;Cho, Hyeoun Suk;Hwang, Jae Bok;Ku, Bon il;Kim, Hag Sin;Seo, Myung Chul;Park, Hong Kyu;Lee, Keon Hui
    • Weed & Turfgrass Science
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    • v.4 no.3
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    • pp.151-158
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    • 2015
  • This study was conducted to establish the effective weed management methods in rice field simultaneously dominated by the herbicide resistant Echinochloa oryzicola, Monochoria vaginalis and Scirpus juncoides. Herbicides registered for use before transplanting, oxadiazon 12% EC, pyrazolate 36% SC, pretilachlor 14% EC and thiobencarb 50% EC were effective until 0.5 leaf stage of herbicide resistant Echinochloa oryzoides. Herbicides registered for use after transplanting, fentrazamide 1% GR and mefenacet 18% SC were effective until 2 leaf stage of herbicide resistant Echinochloa oryzicola and triafamone 0.98% SC was possible to control up to 4 leaf stage. HPPD inhibitors, benzobicyclon, mesotrione and tefuryltrione SC, were simultaneously effective to SU herbicide-resistant Monochoria vaginalis and Scirpus juncoides. Herbicides registered for use before transplanting, benzobicyclon + oxadiargyl EC out of the tested herbicide was most effective in rice field simultaneously dominated by the herbicide resistant Echinochloa oryzicola, Monochoria vaginalis and Scirpus juncoides. Its effectiveness rises in proportion to flooding duration. Mazosulfuron GR, a herbicides registered for use after transplanting was most effective without phytotoxicity until 60 days after transplanting in rice field simultaneously dominated by the herbicide resistant Echinochloa oryzicola.