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Verification of Anti-Inflammatory Efficacy of Apple Mango (Mangifera indica L.) Peel in LPS-Activated Macrophage (LPS에 의해 활성화된 대식세포에서 애플망고 껍질(Mangifera indica L. Peel)의 항염증 효능 검증)

  • Hyo-Min Kim;Dan-Hee Yoo;In-Chul Lee
    • Microbiology and Biotechnology Letters
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    • v.50 no.3
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    • pp.337-346
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    • 2022
  • The purpose of this study was to investigate the antioxidant and anti-inflammatory activities of hot water (AMPW) and 70% ethanol (AMPE) extracts of apple mango (Mangifera indica L.) peel. The antioxidant activities were measured using a total polyphenol, electron-donating, 2,2'-azinobis [3-ethylbenzothiazoline6-sulfonic acid] (ABTS) radical scavenging assay. The total polyphenol content of AMPW and AMPE was 66.08 ± 0.62 mg TAE/100 g and 100.13 ± 0.23 mg TAE/100 g, respectively. As a result of measuring the electrondonating ability, at a concentration of 1,000 ㎍/ml, AMPW and AMPE showed an effectiveness of 86% and 94%, respectively. The ABTS assay showed 80% and 98% respective radical scavenging activity for AMPW and AMPE, at a concentration of 1,000 ㎍/ml. The cell viability on macrophage cells was performed using a 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay, and the results showed more than 90% cell viability at a 100 ㎍/ml concentration. Anti-inflammatory activity was verified by confirming nitric oxide (NO) production inhibitory activity, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) protein and mRNA expression inhibitory activity from lipopolysaccharide (LPS)-treated RAW 264.7 cells. The NO production inhibitory effects were measured using the Griess assay, which confirmed 45% and 40% inhibition after treatment with AMPW and AMPE, respectively. Moreover, the protein and mRNA expression of inflammatory-related factors iNOS and COX-2, decreased in a concentrationdependent manner. In conclusion, this study showed antioxidant and anti-inflammatory effects of Mangifera indica L. peel and revealed its promising potential for application as an antioxidant and anti-inflammatory agent.

Study of Spatiotemporal Variations and Origin of Nitrogen Content in Gyeongan Stream ( 경안천 내 질소 함량의 시공간적 변화와 기원 연구)

  • Jonghoon Park;Sinyoung Kim;Soomin Seo;Hyun A Lee;Nam C. Woo
    • Economic and Environmental Geology
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    • v.56 no.2
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    • pp.139-153
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    • 2023
  • This study aimed to understand the spatiotemporal variations in nitrogen content in the Gyeongan stream along the main stream and at the discharge points of the sub-basins, and to identify the origin of the nitrogen. Field surveys and laboratory analyses, including chemical compositions and isotope ratios of nitrate and boron, were performed from November 2021 to November 2022. Based on the flow duration curve (FDC) derived for the Gyeongan stream, the dry season (mid-December 2021 to mid-June 2022) and wet season (mid-June to early November 2022) were established. In the dry season, most samples had the highest total nitrogen(T-N) concentrations, specifically in January and February, and the concentrations continued to decrease until May and June. However, after the flood season from July to September, the uppermost subbasin points (Group 1: MS-0, OS-0, GS-0) where T-N concentrations continually decreased were separated from the main stream and lower sub-basin points (Group 2: MS-1~8, OS-1, GS-1) where concentrations increased. Along the main stream, the T-N concentration showed an increasing trend from the upper to the lower reaches. However, it was affected by those of the Osan-cheon and Gonjiamcheon, the tributaries that flow into the main stream, resulting in respective increases or decreases in T-N concentration in the main stream. The nitrate and boron isotope ratios indicated that the nitrogen in all samples originated from manure. Mechanisms for nitrogen inflow from manure-related sources to the stream were suggested, including (1) manure from livestock wastes and rainfall runoff, (2) inflow through the discharge of wastewater treatment plants, and (3) inflow through the groundwater discharge (baseflow) of accumulated nitrogen during agricultural activities. Ultimately, water quality management of the Gyeongan stream basin requires pollution source management at the sub-basin level, including its tributaries, from a regional context. To manage the pollution load effectively, it is necessary to separate the hydrological components of the stream discharge and establish a monitoring system to track the flow and water quality of each component.

Comparison of Image Quality among Different Computed Tomography Algorithms for Metal Artifact Reduction (금속 인공물 감소를 위한 CT 알고리즘 적용에 따른 영상 화질 비교)

  • Gui-Chul Lee;Young-Joon Park;Joo-Wan Hong
    • Journal of the Korean Society of Radiology
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    • v.17 no.4
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    • pp.541-549
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    • 2023
  • The aim of this study wasto conduct a quantitative analysis of CT image quality according to an algorithm designed to reduce metal artifacts induced by metal components. Ten baseline images were obtained with the standard filtered back-projection algorithm using spectral detector-based CT and CT ACR 464 phantom, and ten images were also obtained on the identical phantom with the standard filtered back-projection algorithm after inducing metal artifacts. After applying the to raw data from images with metal artifacts, ten additional images for each were obtained by applying the virtual monoenergetic algorithm. Regions of interest were set for polyethylene, bone, acrylic, air, and water located in the CT ACR 464 phantom module 1 to conduct compare the Hounsfield units for each algorithm. The algorithms were individually analyzed using root mean square error, mean absolute error, signal-to-noise ratio, peak signal-to-noise ratio, and structural similarity index to assess the overall image quality. When the Hounsfield units of each algorithm were compared, a significant difference was found between the images with different algorithms (p < .05), and large changes were observed in images using the virtual monoenergetic algorithm in all regions of interest except acrylic. Image quality analysis indices revealed that images with the metal artifact reduction algorithm had the highest resolution, but the structural similarity index was highest for images with the metal artifact reduction algorithm followed by an additional virtual monoenergetic algorithm. In terms of CT images, the metal artifact reduction algorithm was shown to be more effective than the monoenergetic algorithm at reducing metal artifacts, but to obtain quality CT images, it will be important to ascertain the advantages and differences in image qualities of the algorithms, and to apply them effectively.

Daesoon Jinrihoe Yeoju Headquarters Temple Complex as Viewed within Feng-Shui Theory (풍수지리로 본 대순진리회 여주본부도장)

  • Shin, Young-dae
    • Journal of the Daesoon Academy of Sciences
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    • v.33
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    • pp.91-145
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    • 2019
  • This study aims to reveal that Daesoon Jinrihoe Yeoju Headquarters Temple Complex is a sacred place of Gaebyeokgongsa (the Reordering Works of the Great Opening) through the logic of the energy of form in Feng-Shui studies. The Headquarters Temple Complex can illuminate the lamp of coexistence, emerge as a place for cultivation, and support the era of human nobility with Gucheonsangje (the Supreme God of the Ninth Heaven) as an object of faith. Virtuous Concordance of Yin and Yang, Harmonious Union between Divine Beings and Human Beings, the Resolution of Grievances for Mutual Beneficence, and Perfected Unification with Dao are the mission statements of this great site. For this purpose, it is necessary to investigate the headquarters according to integral Feng-Shui Theory. Doing so can provide proof that the geographic location, landscape, yin-yang harmonizing, and flowing veins of terrestrial energy at Headquarters Temple Complex are all profoundly auspicious. At the same time, this data also allows further study into the interactions of dragon-veins, energy hubs, surrounding mountains, and watercourses, which reveal how Daesoon Jinrihoe Yeoju Headquarters Temple Complex promotes the basic works of propagation, edification, and cultivation and three societal works of charity aid, social welfare, and education for the purpose of global propagation, saving beings, and building an earthly paradise by reforming humanity and engaging in spiritual civilization. This must be done on site with proper Feng-Shui in order to open up the era of human nobility upon the Great Opening of the Later World. As the center of the religious order, Daesoon Jinrihoe, Yeoju Headquarter Temple Complex has the general Feng-Shui characteristic of Baesanimsu (a back supported by a mountain and a front facing water). Through discussing the Feng-Shui of Daesoon Jinrihoe's Yeoju Headquarters Temple Complex as the center of humankind's resolution of grievances for mutual beneficence, this study would explore growth-supporting land that delivers future rewards through Feng-Shui symbolism and the ethical practice of grateful reciprocation of favors for mutual beneficence. This exploration will reveal how the geographical features and conditions of the Yeoju Headquarters Temple Complex make it a place fit for spiritual cultivation. It is a miraculous luminous court surrounded by mountains, where auspicious signs in eight directions gather. Its veins of terrestrial energy harmonize with clean water energy as it is affectionately situated within its natural environment. Its location corresponds with the Feng-Shui theory of dragon-veins, energy hubs, surrounding mountains, and watercourses. Thus, with regards to the Feng-Shui of Daesoon Jinrihoe's Yeoju Headquarters Temple Complex, this study examines the flows of mountains and waters and focuses on how the site is based on the logic of Feng-Shui. More generally, the geographical features of the surrounding mountains are likewise examined. An analysis of the relationship between Poguk (布局) of Sasinsa (animal symbols of the four directions, four gods, including blue dragon of the east, red phoenix of the south, white tiger of the west, and black tortoise of the north) and the location will be provided while focusing on the Yeoju Headquarters Temple Complex. This study supports the feasibility of further Feng-Shui studies of the Yeoju Headquarters Temple Complex based on traditional geomancy books that focusing on Hyeonggi (Energy of Form) Theory.

A Study on the Fengshui Shapes of the Four Propitious Sites Recorded in The Canonical Scripture (『전경』에 기록된 사명당(四明堂)의 풍수 물형(物形) 연구)

  • Shin Young-dae
    • Journal of the Daesoon Academy of Sciences
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    • v.41
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    • pp.133-178
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    • 2022
  • This study investigates if four propitious sites (四明堂, sa myeongdang) identified in Daesoon Jinrihoe's The Canonical Scripture correspond with the orthodox descriptions of dragon, energy hub, sand, and water (龍穴砂水, yong hyeol sa su) which are held by Fengshui as conditions necessary for that specific designation. In this study, these conditions, based on the shape of the site-formations, were observed via through on-site surveys and the application of the theories presented by traditional books on Fengshui. First, the dragon veins (龍脈, yongmaek) of the energy hub of the Five Immortals Playing Baduk (五仙圍碁穴 oseonwigi- hyeol) on Mount Hoemun in Sunchang consists of solid soil, is like a spiderweb, rises and lays prone, winds in every direction, and looks almost disconnected while actually remaining connected. Second, the Fengshui characteristics of the energy hub of Worship Held by Buddhist Monks from Abroad (胡僧禮佛穴, hoseungyebul- hyeol) on Mount Seungdal in Muan is that the branching feet (枝脚 jigak) support the mountain range by forming a valley to the left and right of the dragon veins that stretch from the peak of Mount Seungdal. Also, the direction-changing helm (橈棹 yodo) supports the mountain range solidly can be said to be well-developed. It is likewise noted that there is an excellent change in dragon veins in that exhibit curvature that spans being high, low, rising, and lying. This makes it appear as though the dragon is wriggling back and forth. Third, the state of the energy hub of Celestial Maidens Weaving Silk (仙女織錦穴, seonnyojikgeum-hyeol) on Sonryong Ridge in Jangseong County demonstrates overall harmony between mountain and water as it is near Mount Ju and Mount An and has a solid water outlet to which it is tightly fastened such that its energy does not leak out. Meanwhile, the positioning of its blue dragon of the east, red phoenix of the south, white tiger of the west, and black tortoise of the north is so intimate that its long flow can be said to be spinning. The Songryong Ridge area where energy hub was formed between soil and bedrock is the right land for a great favored location as it corresponds with Fengshui logic in an exemplary manner. Fourth, the Fengshui characteristics of the energy hub of Subjects Receiving the Imperial Command (群臣奉詔穴, gunshinbongjo-hyeol) at Baerye-jeon Field in Taein can be described as embracing the village snuggly as it centers around Mount Wangja, and its blue dragon and white tiger respond to each other. Additionally, a clear distinction between host and guest is noticeable in the positions of Mount Ju and Mount An. The flowing body of water in front of the village wraps around that village as a Horizontal Water Formation (橫水局, hoengsuguk), and vigorous vapor from the earth draws breath as the yin-yang energy of the landscape courses through the earth. As dragon veins, the range of the mountain, are like the blood vessels within the human body and the dragon is compared to the limbs, the energy hub of Subjects Receiving the Imperial Command at Baerye-jeon Field in Taein can be identified as a favored location that was formed directly by the sky and earth.

Growth Characteristics and Yields of Native Summer Forage Crops as Affected by Seeding Rate in No-Tillage Paddy Fields (무경운 논에서 하계 자생사료작물의 파종량이 생육특성 및 수량에 미치는 영향 )

  • Jeong Sung Jung;Bo Ram Choi;Se Young Lee;Mirae Oh;Hyung Soo Park;Ki Choon Choi
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.1
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    • pp.28-34
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    • 2023
  • This experiment was carried out to determine effects of seed rate of native grasses, called native summer forage crops (NSFC), on their growth characteristics and yields in no-tillage paddy fields in central provinces. NSFC cultivation was performed according to the agricultural guidelines of the Rural Development Administration (RDA) during 2020 to 2022. Echinochloa crusgalli 'Jeju native', Setaria viridis 'Korean native type', Digitaria sanguinalis 'Korean native type') were sown by various seed rate in the experimental field (3m × 4m plot) of Department of Animal Resources Development, Seonghwan-eup, Cheonan, Korea in late-May or early-June for 3 years and harvested in middle-September. Dry matter yields (DMY) in Echinochloa crusgalli and Setaria viridis increased as increasing seed rate. DMY in 40 kg ha-1 of Echinochloa crusgalli was the highest and DMY in 60 kg ha-1 of Setaria viridis was the highest. However, All treatments of Digitaria sanguinalis showed similar DMY. Yields of NSFC were the order of Echinochloa crusgalli (9,681~11,092 kg ha-1) > Digitaria sanguinalis (8,311~9,023 kg ha-1) >etaria viridis (3,145~4,749 kg ha-1). The contents of crude protein in NSFC were the order of Digitaria sanguinalis > Echinochloa crusgalli > Setaria viridis. The contents of ADF and NDF in NSFC were the order of Echinochloa crusgalli > Digitaria sanguinalis > Setaria viridis. As shown in above results, we suggest that introduction of NSFC is one of the most important skills in order to stably increase yields of forage crops for utilization of the double cropping system in no-tillage paddy fields.

Study on Forage Cropping System Using Summer Forage Crops with Italian Ryegrass at Paddy Land of Gangjin Region in South Korea (남부지역 논에서 이탈리안 라이그라스와 하계 사료작물을 연계한 작부체계에 관한 연구)

  • Jeong Sung Jung;Se Young Lee;Mirae Oh;Hyung Soo Park;Ana Yun;Ki Choon Choi
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.1
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    • pp.35-41
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    • 2023
  • This experiment was carried out to examine double cropping system using Italian ryegrass (IRG) 'Kowinearly', whole crop rice 'Yeongwoo' and barnyard millet 'Jeju native' during the Oct. 2020 to Oct. 2022. Three kinds of forage crops were cultivated at paddy field in Livestock Institute, Jeollanamdo Agricultural Research and Extension Services, Jeollanamdo, South Korea. Whole crop rice (WCR) was sown in late May and barnyard millet (BM) sown early June each year after harvesting IRG. We examined dry matter yield and feed value of forage crops depending on harvest time of forage crops during the experimental period. The plant height in heading stage of IRG ranged from 108 to 112cm and dry matter yield ranged from 6,783 to 11,530 kg ha-1. The crude protein (CP) of IRG ranged from 6.0 to 8.44%, acid detergent fiber (ADF) ranged from 55.6 to 60.2% and neutral detergent fiber (NDF) ranged from 32.58 to 36.7%, The dry matter yield of WCR increased as the harvest stage was delayed (14,310 kg ha-1 in milk, 16,167 kg ha-1 in yellow ripen, and 18,891 kg ha-1 in mature). Similar to results of dry matter yield of WCR, dry matter yield of BM increased as the harvest stage was delayed (11,194 kg ha-1 in late heading. and 14,308 kg ha-1 in mature), However nutrient content of WCR and BM showed a decreasing trend. As shown in above results, the productivity of WCR after harvesting IRG was shown to be high at paddy field in the southern region. However, BM also was appeared to have potential as summer forage crops.

Correlation Analysis among Peer Evaluation, Self-evaluation and Instructor Evaluation on Argumentation Writing in the 'Biology Logic and Essay' ('생물교과논리 및 논술' 에서의 논증글쓰기에 대한 동료평가-자기평가-교수자 평가 간 상관관계 분석)

  • Kyunghee Kang
    • Journal of Science Education
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    • v.47 no.1
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    • pp.75-87
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    • 2023
  • This study analyzed correlation among peer evaluation, self-evaluation, and instructor evaluation of argumentation writing conducted in the 'Biology logic and essay' class. To this end, 14 pre-service biology teachers wrote argumentation four times, and peer evaluation, self-evaluation, and instructor evaluation were conducted on these results. The peer evaluation for argumentation writing averaged 2.70 points, the self-evaluation average was 2.68 points, and the instructor evaluation average was 2.71. Looking at the average of each sub-evaluation area, scientific knowledge was the highest in peer evaluation, and the composition area was the highest in self-evaluation. In addition, the area of scientific knowledge was the highest in the instructor evaluation. As a result of examining the standard deviation, the standard deviation of self-evaluation was the largest. As a result of analyzing the correlation between evaluation types, there was a high correlation between peer evaluation and instructor evaluation, and there was a moderate correlation between self-evaluation and instructor evaluation. On the other hand, there was no significant correlation between peer evaluation and self-evaluation. As a result of analyzing the correlation by evaluation area, there was no significant correlation between peer evaluation and self-evaluation in all four areas of scientific knowledge, scientific argumentation, composition, and expression. On the other hand, in the analysis between peer evaluation and instructor evaluation, there was a significant correlation in the areas of scientific knowledge, scientific argumentation, and composition. In addition, there was a significant correlation between self-evaluation and instructor evaluation only in the area of expression. The results of this study show that peer evaluation is highly likely to be applied in the 'Subject logic and essay' class. It also suggests that it is necessary to subdivide evaluation criteria according to the type of evaluation in order to use peer evaluation and self-evaluation.

Current status of cherry trees (Prunus subg. Cerasus) planted in Korea: A case study of Bundang Central Park and adjacent area (국내 벚나무류(Prunus subg. Cerasus) 식재 현황: 분당중앙공원 일대 사례연구)

  • HAN, Byungwoo;JUNG, Jongduk;NA, Hye Ryun;KANG, Kyoungsuk;CHANG, Hany;KIM, Seryoung;KIM, Youme;KWON, Heejeong;HYUN, Jin-Oh
    • Korean Journal of Plant Taxonomy
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    • v.52 no.1
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    • pp.54-63
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    • 2022
  • Prunus subg. Cerasus is the most planted street and landscaping tree in South Korea, but it is difficult to identify species according to their macro-morphologies, leading to problems when attempting properly to manage species quantities. The purpose of this study is to understand the current status of plant types and species compositions in Bundang Central Park in Bundang-gu of Seongnam City and to discuss the necessity of the management of landscaping tree planting. In April of 2021, during the cherry blossom season, a total of 5,866 planted cherry trees were investigated within an area of 6 km2 of Bundang-gu in Seongnam City. As a result, 5,744 trees were sorted into eleven taxa, but the remaining 122 trees were not determined due to their complex morphologies. Prunus ×yedoensis Matsum. accounted for the highest proportion (52.1%), followed by P. serrulata Lindl. var. pubescens Nakai and P. jamasakura Siebold ex Koidz. P. ×nudiflora (Koehne) Koidz., a plant native to Jejudo Island, was not found in this survey. In order to help identify cherry trees based on micro-morphologies, an identification key was presented for the eleven taxa planted as major landscaping trees. It is known that cherry trees frequently form interspecific hybrids in nature. In order to prevent a loss of the genetic originality of native species due to hybridization and gene introgression from foreign cherry trees, it is necessary to manage planting species near the habitats of native taxa and track their origins.

Analysis of Pedigree Structure and Inbreeding Coefficient for Performance Tested Holstein Cows in Korea (우리나라 Holstein 능력검정 젖소 집단의 혈통구조 및 근교계수 분석)

  • Won, J.I.;Dang, C.G.;Lim, H.J.;Jung, Y.S.;Im, S.K.;Lee, J.K.;Kim, J.B.;Cho, M.R.;Min, H.L.;Yoon, H.B.
    • Journal of agriculture & life science
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    • v.50 no.2
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    • pp.107-116
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    • 2016
  • The study was aimed to analyze pedigree structure and inbreeding coefficients for performance tested Holstein cows in Korea. A total of 400,029 Holstein cows data which born between 2002 and 2012 were obtained from Dairy Cattle Improvement Center of National Agricultural Cooperative Federation(NACF). Their related pedigrees, as obtained from Korean Animal Improvement Association(KAIA), consisted of 509,740 animals. Pedigree depth of the cows were traced back to 3 generations earlier. The percentage of cows with fully identified ancestors in various provinces of Korea were 55.18%(Gyeonggi-do), 23.49%(Gangwon-do), 47.83%(Chungcheongnam-do), 53.62%(Chungcheongbuk-do), 56.38%(Gyeongsangbuk-do), 51.35% (Gyeongsangnam-do), 26.58%(Jeollanam-do), 49.41%(Jeollabuk-do), and 56.90%(Jeju-do), whereas, it was about 63.20% as a whole in Korea. The average inbreeding coefficients showed increment across the consecutive years of birth such as, 0.43(2002), 0.44(2003), 0.58(2004), 0.64(2005), 0.78(2006), 0.93(2007), 1.08(2008), 1.23(2009), 1.46(2010), 1.77(2011), and 2.03 (2012). However, this coefficient was 0.93 in overall Korean population. An average generation interval for sire to daughter genetic path was 8.15 years; which was about 4.20 years considering dam to daughter genetic path. The estimated effective population sizes (Ne) were 56.5, 51.3, and 32.2 animals born in 2004, 2009, and 2012, respectively. These results indicated that an increased rate of inbreeding has led to a significant reduction in the Ne over the decade.