• Title/Summary/Keyword: 정확검정

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Enhancement of Geomorphology Generation for the Front Land of Levee Using Aerial Photograph (항공영상을 연계한 하천 제외지의 지형분석 개선 기법)

  • Lee, Geun Sang;Lee, Hyun Seok;Hwang, Eui Ho;Koh, Deuk Koo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.28 no.3D
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    • pp.407-415
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    • 2008
  • This study presents the methodology to link with aerial photos for advancing the accuracy of topographic survey data that is used to calculate water volume in urban stream. First, GIS spatial interpolation technique as Inverse Distance Weight (IDW) and Kriging was applied to construct the terrain morphology to the sand-bar and grass area using cross-sectional survey data, and also validation point data was used to estimate the accuracy of created topographic data. As the result of comparison, IDW ($d^{-2}_{ij}$, 2nd square number) in Sand-bar area and Kriging Spherical model in grass area showed more efficient results in the construction of topographic data of river boundary. But the differences among interpolation methods are very slight. Image classification method, Minimum Distance Method (MDM) was applied to extract sand-bar and grass area that are located to river boundary efficiently and the elevation value of extracted layers was allocated to the water level point value. Water volume with topographic data from aerial photos shows the advanced accuracy of 13% (in sand-bar) and 12% (in grass) compared to the water volume of original terrain data. Therefore, terrain analysis method in river linking with aerial photos is efficient to the monitoring about sand-bar and grass area that are located in the downstream of Dam in flooding season, and also it can be applied to calculate water volume efficiently.

Simultaneous determination of amphetamine-like drugs in human urine by SPE and GC/MS (고체상추출과 GC/MS를 이용한 소변 중 암페타민계 마약성분 동시분석법)

  • Cheong, Jae Chul;Kim, Jin Young;In, Moon Kyo;Cheong, Won Jo
    • Analytical Science and Technology
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    • v.21 no.1
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    • pp.41-47
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    • 2008
  • Although liquid-liquid extraction (LLE) method has been used routinely for the analysis of amphetamine-like drugs (amphetamine; AP, methamphetamine; MA, 3,4-methylenedioxyamphetamine; MDA, 3,4-methylenedioxymethamphetamine; MDMA, 3,4-methylenedioxyethylamphetamine; MDEA), a solid-phase extraction (SPE) method, which can be automated, was applied for the simultaneous determination by GC/MS in human urine. Urine samples (3 mL) and 0.1 M phosphate buffer (1 mL, pH 7.0) were extracted by an automated SPE system. The eluent was evaporated, derivatized with trifluoroacetic anhydride (TFAA), and analyzed by GC/MS. The calibration curves was linear with correlation coefficient ($r^2$) above 0.994 in the ranges of 34.0 (AP), 28.0 (MDA)~1000.0 ng/mL for AP, MDA, and 50.0~2000.0 ng/mL for MA, MDMA, and MDEA. The limits of detection ranged from 4.0 to 10.0 ng/mL, and the limits of quantitation ranged from 12.0 to 34.0 ng/mL. The relative recoveries were 93.5~107.7 %. The precisions and accuracies were 1.9~14.8 % and -8.7~14.8 %, respectively. The present method was successfully applied to identify the MA or Ecstasy (MDMA) abusers in exact as well as rapid.

The Comparative Analysis of Reservoir Capacity of Chungju Dam based on Multi Dimensional Spatial Information (다차원 공간정보 기반의 충주댐 저수용량 비교분석)

  • Lee, Geun Sang
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.5D
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    • pp.533-540
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    • 2010
  • Dam is very important facility in water supply and flood control. Therefore study needs to analyze reservoir capacity accurately to manage Dam efficiently. This study compared time series reservoir capacity using multi-dimensional spatial information to Chungju Dam reservoir and major conclusions are as follows. First, LiDAR and multi beam echo sounder survey were carried out in land zone and water zone of Dam reservoir area. And calibration process was performed to enhance the accuracy of survey data and it could be constructed that multi dimensional spatial information which was clearly satisfied with the standard of tolerance error by validation with ground control points. Reservoir capacity by water level was calculated using triangle irregular network from detailed topographic data that was constructed by linked with airborne LiDAR and multi beam echo sounder data, and curve equation of reservoir capacity was developed through regression analysis in 2008. In the comparison of the reservoir capacity of 2008 with those of 1986 and 1996, the higher water level goes, total reservoir capacity of 2008 showed decrease because of the increase of sediment in reservoir. Also, erosion and sediment area could be analyzed through calculating the reservoir capacity by the range of water level. Especially the range of water level as 130.0~135.0 which is the upper part of average water level, showed the highest erosion characteristics during 1986~2008 and 1996~2008 and it is considered that the erosion of reservoir slant by heavy rainfall is major reason.

Some Comments on the Preparation of the CSK Standard Chemical Solutions (CSK Standard Chemical Solution 과 그에 대한 몇가지 의견)

  • Won, Chong Hun
    • 한국해양학회지
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    • v.4 no.2
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    • pp.83-86
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    • 1969
  • 해양관측의 세계적인 공통성으로 해서 염분검정법이 이미 오래 전부터 통일화 되었지만 근래에 와서는 용존산소량 정량법도 국제적인 Intercalibration을 하는 등규격화에의 기운이 나고 있다. 다시 Kuroshio 합동조사에서는 영양염의 정량에 있어 공통된 표준용액을 사용하므로써 조작상의 편리와 측정치의 신뢰성을 더욱 향상시키자는 의도에서 일본이 국제적인 영양염 표준용액의 조제 및 배포에 관한 안을 내어 1965년 Manila 회의를 거쳐 일본 상모중앙화학연구소의 관원씨가 이를 맡아 1966년부터 시작하여 1967년까지에 요오드산칼리움, 아질산염, 인산염, 규산염의 표준용액을 만들어 1968년 봄부터 시험적으로 일본국내와 동남아 수개국에 나누어 사용해 왔던 것이다. 다시 1968년 9월의 SCOR 의 영양염에 관한 Working Group 회의에서 CSK Std. Solution을 사용하여 세계각국에서 현재 사용하고 있는 영양염 분석방법의 Intercalibration을 하자는 회의가 있었고, 이것을 권고사항으로 SCOR에 보고하여 1968년 11월에 ICES가 승인하므로써 Intercalibration에 관한 원칙이 정해졌다. 동시에 Finland의 Koroleff씨와 Palmork씨가 organizer로 정해졌던 것이다. 이 보다 약간 앞서 본인이 상모중연에 가 있을 때 Std. Solution으로서 아질산염용액 만으로 각종무기질소화합물의 표준용액으로 대용한다는 것은 비합리적이므로 질산염과 암모늄염의 표준용액이 있어야 한다고 주장하여 우선 질산염용액을 추가로 만들기로 하여 1968년 11월부터 표준물질의 정제부터 시작 되었다. 1969년 1월에 Intercalibration 에 관한 구체적인 회의를 위해 Scripps 해양연구소에 관원, Wooster Rakestraw, Cieskes씨등이 모여 우선 일본상모중연에서 만들고 있는 인산염, 질산염, 아질산염, 규산염의 CSK 표준용액을 표준시료로하여 SCOR 과 ICES의 해양화학분과에서 선정한 세계 100개처에 나누어 현재 각자가 사용하고 있는 방법의 정밀도와 정확도를 check하는 소위 International intercalibration을 1969년 9월부터 시작하기로 확정을 보았고, 동시에 구체적인 지시가 있었던 것이다. 이시료를 받는 사람에게는 다만 그것의 농도범위만 알려주고 정확한 농도는 Koreleff와 관원씨만이 알고 있기로 하여 측정에 분석자의 주관이 개입되지 못하도록 했고, 분석치는 SCOR가 모아 해석하되 번호제로 하여 어떤 나라의 누구가 했다는 것은 밝히지 않기로 되어 있다. 이같은 내력으로 CSK Std. Solution이 국제적인 Intercalibration용의 표준시료로서 시험적으로 사용되기 까지는 되었으나, CSK Std. Solution 그자체에 관해서는 아직도 해결해야 할 점, 개량을 요하는 점이 많다. 이하에서는 주로 개량을 요하는 점에 관해 몇가지 언급하고자 한다.

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메기 Silurus asotus 발생난의 핵분열

  • 임재현;박인석;허준욱;정지혜;김동수
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.30-30
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    • 2003
  • 자성발생(gynogenesis)은 대상 어류의 성 결정기작이 암컷 동형접합성(female homogamety)인 경우 전 암컷 단성집단을 생산할 수 있으며, 단기간 내에 우수한 품종의 순계 획득과 유전적으로 동일한 clone 집단을 만들 수 있다(Thorgaard, 1986; 정 등 1996). 이에 제 1 난할 억제에 의한 메기 S. asotus에서의 체세포분열 억제성(mitotic) 자성발생 2배체가 유도된 바 있다(임 등, 2002), 염색체공학 기법 중 웅성발생 2배체(androgentic diploid)는 난자 핵을 불활성 시킨 후 정자 핵에 의해 제 1 난할억제에 의한 유전자 배가로 유도 될 수 있다. 본 연구는 염색체공학에 의한 메기 4배체 생산, 메기체세포분열 억제성 자성발생성 2배체의 효과적인 유도 조건 검정 및 메기 웅성발생성 2배체 생산을 위한 연구의 일환으로, 메기에서의 핵분열(korykinesis)에 관하여 조사하였다. 메기에서의 핵분열 수온 25$\pm$0.5$^{\circ}C$ 조건에서 수정 후 31분에 가장 현저하였다. 본 연구 결과를 Park and Im (2001)의 수온 24$^{\circ}C$에서의 제 1 난할 후기가 50분이며 mitotic interval이 18.5$\pm$1.2분인 결과와 비교시, 본 연구 결과의 수온 24$\pm$0.5$^{\circ}C$ 조건에서 핵분열이 수정 후 31분인 것은 핵분열과 세포질분열(cytokinesis)이라는 관점에서 정확히 일치한다. 염색체 수의 배가시, 핵분열 억제는 세포질 분열 억제에 비해 더욱 효과적으로서(Cherfas et al., 1993; Nam et al., 1999), 본 연구 결과의 핵분열 시간(수온 24$^{\circ}C$ 조건에서의 수정후 31분)을 기반으로 하여 차후, 4배체 메기와 체세포분열 억제성 자성발생 2배체 메기 및 웅성발생성 2배체 메기 유도 및 그의 생산성 향상에 관한 연구가 기대된다.

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Pathogenic Races of Pyricularia oryzae Isolated from Various Rice Cultivars on the Blast Nursery and Paddy Field in Different Locations (벼 품종 및 지역에 따른 도열병균 레이스 분리와 도열병 발병정도와의 관계)

  • Shim, Hongsik;Yeh, Wan-Hae;Yoo, Boung-Joo;Myung, Inn-Shik;Hong, Sung-Kee;Lee, Seungdon
    • Research in Plant Disease
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    • v.18 no.4
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    • pp.324-330
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    • 2012
  • This study was conducted to investigate the association of race distribution of Pyricularia oryzae with rice cultivar and location. Races present in a given location were different in cultivars, and the races from a given cultivars were different by location. For precise study of race distribution, it needs to identify isolates by considering cultivation ratio and collection area. There are great differences between the resistant degree in the upland blast nursery and on the resistant degree of the leaf blast and panical blast in the paddy field. The number of virulent races in a rice cultivar was not related with the resistant degree in blast nursery or in paddy field.

Evaluations of the Accuracy and Reliability of Measurements Made on White Light Scanner-based Dental Digital Models (백색광 스캐너로 채득된 치과용 디지털모형의 정확도와 신뢰도 평가)

  • Kim, Ki-Baek;Kim, Jae-Hong
    • The Journal of the Korea Contents Association
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    • v.12 no.10
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    • pp.357-364
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    • 2012
  • Dental scanner-based dental digital models have been developed that have the potential to replace conventional stone model. The aim of this study was examine the accuracy and reliability of measurements made on digital models. A master model with the prepared upper full arch tooth was used. Stone model(N=10) were produced from master model, and on the other hands, digital models were made with the white light scanner(Identica, korea). One examiner individually measured 6 parameters on the conventional model and the digital models on two occasions. The student's t-test for paired samples and intra-class correlation coefficient(ICC) were used for statistical analysis. At the intra-examiner reliability of measurement, ICC at the stone and digital models were 0.75 and 0.87. The mean difference between measurements made directly on the stone models and those made on the digital models was 0.11-0.23mm, and was statistically significant(P<0.05). These in vitro studies show that accuracy of the digital model is similar to that of the stone model. These results will have to be confirmed in further clinical studies.

Accuracy Improvements of DAM-Reservoir Storage Volume Estimation based on GIS (GIS기반에서 댐 저수량 산정의 정확도 향상)

  • Jeong, InJu;Moon, DooYoul;Jung, BeomSeok
    • Journal of the Korean Association of Geographic Information Studies
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    • v.8 no.2
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    • pp.136-144
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    • 2005
  • GIS analyzes various topographic feature in three dimensions using triangulated irregular network (TIN). This paper proposed a method that can analyze topographic features effectively in three dimensions. Method developed in this study can calculate earth-volume, the inundation volume, storage volume etc, effectively. We could reduce error than existing volume calculation methods in computing volume in addition to water level's change about each. Also, the develop method can improve accuracy in measuring the storage of reservoirs. Main result of this paper id to develop a efficient calculation method using contour and elevation data on digital map.

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Development and of Diagnostic System for Detection of Cowpea chlorotic mottle virus using by Nested PCR (Nested PCR을 이용한 Cowpea chlorotic mottle virus 정밀 진단 시스템 개발)

  • Min, Byung-Dae;Kim, Young-Suk;Lee, Siwon;Lee, Su-Heon
    • Korean Journal of Agricultural Science
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    • v.41 no.4
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    • pp.335-339
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    • 2014
  • Cowper chlorotic mottle virus (CCMV) is the 'controlled' quarantine virus as plant pathogenic virus that are classed as group VI (+) ssRNA virus that belongs to the genus Bromovirus and family Bromoviridae, When plants that are Phaseolus vulgaris, Clitoria ternatea, Nicotiana tabaccum, Glycine max, Vigna unguiculata and Vigna siensis, and Arachis hypogaea is imported in domestic. In this study, inspection system is implemented to analyze CCMV accurately and rapidly by developing RT-PCR, nested PCR, and gene insertion positive control. It is expected that the method developed in this study will contribute to the plant quarantine to be consistently utilized in the field.

Determination of the Cr2O7 Ion by a New Absorption Spectrometric Method Coupled with a Technique of Flow Injection Analysis (흐름주입분석기법을 접목한 새로운 흡수분광분석법에 의한 Cr2O7 이온의 정량)

  • Hwang, Hoon
    • Journal of the Korean Chemical Society
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    • v.44 no.4
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    • pp.322-327
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    • 2000
  • A new absorption spectrometric method for the determination of the Cr$_2O_7^{2-}$ ionin acidic media has been developed. The new method is based on the oxidation-reduction reaction of the HCr$O_4^-$ ion with H$_2$O$_2$forming a deep blue CrO(O$_2$) $_2$ andis coupled with a technique of flow injection analysis(FIA). The new method provides a linear calibration curve which accurately follows the Beer's law over a wide range of the analytical concentrations(2.0 ${\times}$ $10^{-6}$M~8.0 ${\times}$ $10^{-3}$M) of K$_2$Cr$_2$O$_2$. The sensitivity of the new method is approximately two times greater than the current method and the effects of the interfering substances such as V, Co, Ni, Fe, and Mn are almost negligible except Cu.

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