• Korean Journal of Microbiology
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• v.30 no.4
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• pp.291-298
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• 1992

Srrepromycec. corlirolar produces at least 4 catalase activity bands with different electrophoretic mobilities on polyacrylamide gel which vary during development. Spores and mycelia at stationary phase produced all the activity bands(Cat1. 760 kr); Cat3-I, 170 kD: Cat3-2, 140 kD: Cat3-3. 130 kD; Cat4, 70 kD) except for Cat2 (300 kD). Mycelia at mid-logarithmic phase produced only Cat2 and Cat3-2 bands, and mycelia at late-logarithmic phase produced bands except Catl and Cat\ulcorner. Catalase-deficient mutants were screened in S. coelicalur by H201 bubbling test following NTG mutagenesis. Wc tested sevcral non-bubbling or slow-bubbling mutants for their catalase activities. The overall activities in cell extracts decreased more than 5 fold. Activity bands in native gel selectively decreased in intensity or disappeared. In all the non-bubbling mutants testcd, Cat3-2 band decreased significantly or disappeared. suggesting that Cat3-2 is the major catalase. The selective disappearance of bands in mutants suggest that each band is governed by different genes. We purified catalase activity from -:ell extracts obtained at late-logarithmic phase. Following chromatographies on Sepharose CL-4B. DEAE Sepharose CL-6B. Phcnyl Sepharose CL-4B. and hydroxylapatite columns. only the Cat3-2 activity was obtained. The native form of Cat3-2 has molecular weight of approximately 140 kD, judged by gel electrophoresis. Thc electrophoretic mobility on SDS-polyactylamide gel suggests that this enzyme contains 2 identical subunits of 67 kD.

• The Research Journal of the Costume Culture
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• v.13 no.1
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• pp.60-74
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• 2005

The purpose of this study was to systematize accurate data about the body measurements of early adolescent boys, as there was a broad individual variance between their bodies, and it's additionally attempted to identify the unique physical characteristics of different age groups. The subjects in this study were boys who were at the ages of 10 to 14 in the capital area. Their bodies were measured by anthropometric and photographic method, and data on 549 boys were analyzed. The total of 100 items(47 anthropometric items, 10 indexed items and 43 photographic items) were selected to be measured. For data analysis were performed descriptive statistics, ANOVA and Duncan test using SPSS Ver. 10. The results of the study are as follows. According to the result of comparing the body measurements of early adolescent boys, a period of active growth and a period of slow growth were repeated alternately as they aged and the age of active growth tended to get younger.

• Korean Journal of Microbiology
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• v.31 no.2
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• pp.166-174
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• 1993

Streptomyces coeUc%r (Muller) cells were treated with $100 \mu$M hydrogen peroxide for I hour and proteins synthesized during hydrogen peroxide stress were labeled with L-[$^{35}S$]-methionine. Total cellular proteins were extracted and analyzed by two-dimensional polyacrylamide gel electrophoresis. In exponential growth phase, synthesis of about 100 proteins was increased by hydrogen peroxide treatment. These proteins were named as Pin (£eroxide-inducib]e) proteins and classified into 4 subgroups according to their induction time after hydrogen peroxide treatment. About 60 of them were found to be induced within 20 minutes and maintained throughout I hour of treatment. In stationary growth phase. synthesis of 62 proteins was increased by hydrogen peroxide and 21 of them were the same Pins found in exponential growth phase. Proteins from the mutants which are resistant to hydrogen peroxide were obtained in exponential growth phase and compared with those from the wild type on two-dimensional gel. The three mutants, N7, N9. and N24, were found to have higher constitutive leve]s of ]5, 17, and 15 Pin proteins respectively, than the wild type. 9 of these Pin proteins (D74.7a, E76.0c, E23.3. F50.7, F47.2a. F25.5, G39.6b, G24.0, H39.6a) increased in two of the three mutants and 3 proteins (F39.7, H6I.7. 120.8) increased in all of the three mutants. These proteins might play important roles in the response of S. coelic%r to hydrogen peroxide.

• Korean Journal of Ecology and Environment
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• v.33 no.4 s.92
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• pp.395-404
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• 2000

The gonadosomatic index (GSI), hepatosomatic Index (HSI), egg size distribution, gonad development and reproductive characteristics of venus fish, Aphyocypris chinensis, were examined during March 1997- March 1998 in agricultural waterways of the Sedo-myun, Puyo county, Chungnam Province, Korea. Annual reproductive cycle was classified into the following five successive phases by monthly changes in GSI and the characteristics of ovary: quiescent phase (January- February), secondary growing and mature phase (March-May), ripe and spawning phase (June-July), degenerating and resting phase (August-September) and primary growing phase (October-December). The hepatosomatic index (HSI) showed clear seasonal pattern with two separate peaks. However, it exhibited a negative correlation to changes of GSI values. The regression analysis suggested that fecundity showed a strong positive linear relationship ($r^2\;=\;0.91$, n = 34) with body weight. The sex ratio of female to male was 1.4 : 1 in the natural population during the study. The minimum reproductive size of female and male venus fish was 38 mm and 33 mm in fork length, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.3
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• pp.429-435
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• 1994

The effect of methionine (Met) supplementation on glutathione peroxidase(GSHPx) activity in young and 14 month-old rat and mice was investigated. GSHPx activity was more enhanced by methionine supplementation in young rats when selenium (Se) was given as selenite than given in the form of selenomethione (Se-Met). However, GSHPx activity was not influenced by Met supplementation in the old rats. When diets were low in Se, the biopotency of ht eenzyme by Met was facilitated. No significant differences in GSHPx activity was observed with Met supplement in growing mice when Met was given 0.3% and 0.8% iin the diet at high levels of Se (2 ppm). The peak GSHPx in liver and kidney occurred at day 18, thereafter it decreased. Particularly, the liver GSHPx at day 18 increased 4.2 times than that at day 4 by 0.5% Met supplementation, while the unsupplemented group remained only 2.5 times increase. It is considered that in some tissues Met requirement may be met by Se-Met when rats were fed a diet suboptimal in Met. In addition, at lower levels of Se the utilization of Se is more enhanced by Met than at higher levels of dietary Se. Therefore, GSHPx activity may be influenced greatly by Met status along with dietary Se.

• Korean Journal of Culture and Social Issue
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• v.16 no.2
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• pp.125-146
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• 2010

A total of 181 college students(61 males 121 females) with at least 5 years of living abroad (Returnee Group) and another group of 181 students (92 males and 93 females) without extended period of living abroad (Comparison Group) participated in the study by completing a questionnaire consisting of Acculturation Index, Multidimensional Acculturation Scale, Student Adaptation to College Questionnaire, Revised UCLA Loneliness Scale, CES-D, and WHOQOL. The results indicated that the Returnee Group, compared to the Comparison Group, reported as good adjustment toward college life in Korea and positive attitude toward the Korean identity, but a higher level of loneliness. When the Returnee Group were divided into 4 different groups on the basis of acculturation pattern, the Integration and Assimilation Type reported a better adjustment to college life, lower depression and loneliness and better quality of life than the Marginalization Type. The Mariginalization Type appears to be the most vulnerable group, experiencing difficulties in all areas of adjustment, and is clearly in need of interventions. Limitations of the present study and suggestions for future research were discussed.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.15 no.4
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• pp.109-120
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• 2020

This study examines how the government's fairness in entrepreneurship support affects satisfaction and management performance, and examines whether these influences are different for each growth stage of start-up companies. For this study, data were collected for start-up companies that received government support for start-up within the past 5 years. Total 611 copies of the data were used in this study. The collected data were analyzed using SPSS and AMOS. The fairness used in this study was divided into three types, procedural fairness, interactive fairness, and distributed fairness. The effect of the three fairness on the satisfaction of start-up support project was analyzed. In addition, the effect of business support satisfaction on business performance was analyzed, where the business performance was evaluated by the questioner's satisfaction with their business. The start-up phase was divided into the start phase, early growth phase, stagnant phase, and high-level growth phase, and the moderating effect between the fairness and satisfaction of the government-supported projects by start-up phase was analyzed. As a result, it was found that every concept of fairness had a positive (+) effect on the satisfaction of the entrepreneurship support project, and the satisfaction of the entrepreneurship support project had a positive (+) effect on the management performance. The concept of procedural fairness in the start phase, procedural fairness in the early growth phase, interactive fairness, and the concept of all fairness in the stagnant phase influenced the satisfaction of the start-up support project. In this study, the fairness and effect of government-supported projects affecting the management performance of start-ups were identified by growth phase of start-ups. The results of these studies will help build a systematic system for entrepreneurship support and for start-ups, it will also greatly contribute to finding differentiated growth plans by growth stages of start-up companies.

• Microbiology and Biotechnology Letters
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• v.45 no.2
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• pp.149-154
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• 2017

To increase the efficiency of esterase production by Bacillus, high cell-density culture of recombinant Escherichia coli through fed batch fermentation was tested. Cells were cultured to $OD_{600}$ of 76 (35.8 g/l DCW) with dissolved oxygen level controlled to least above 30% air saturation by supplying pure oxygen. Cells were cultured to an $OD_{600}$ of 90 (42.4 g/l DCW) with glucose feeding controlled to at least 1 g/l. However, the cells reached stationary phase at the late stage of culture, despite glucose being supplied. Cells were cultured to an $OD_{600}$ of 185 (87.3 g/l DCW) by supplying additional medium with fortified yeast extract. To increase the productivity of the recombinant protein, cell growth and esterase productivity based on induction time were evaluated. Late exponential phase induction for esterase production in fed batch fermentation resulted in maximum optical density $OD_{600}$ of 190 (89 g/l DCW) and maximum esterase activity of 1745 U/l, corresponding to a 5.8-fold enhancement in esterase production, compared to the early exponential phase induction. In this study, we established fermentation methods for achieving maximum production of Bacillus-derived esterase by optimizing IPTG induction time in high-cell density culture by supplying pure oxygen and a nitrogen source.

• Journal of the Economic Geographical Society of Korea
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• v.21 no.4
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• pp.303-320
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• 2018

This study analyzes the characteristics of the evolution process of the Gumi National Industrial Complex as well as its external and internal drivers based on the cluster adaptation cycle model. The Gumi National Industrial Complex has made remarkable progress through expansion in spatial and industrial realm and has become a representative IT industry cluster in Korea. It evolved during a growth period from the 1990s, a maturity period from the mid-2000s, and a mature stagnation period from the mid-2010s. But it has now entered a period of decline. While external drivers at the international and national level greatly influenced the Gumi National Industrial Complex in its evolution from foundation-building to maturity, internal drivers such as the outflow of large firms as well as a lack of SME research capacity and institutional base have added to the management difficulties of SMEs in the mature stagnation period. Therefore, in order for the Gumi National Industrial Complex to move into a revitalization period that strengthens resilience against external shocks, it is necessary to enhance the capacity of SMEs by expanding the roles of the central government, local government, and support agencies. In addition, it is necessary to create and embed strong medium enterprises within the Gumi National Industrial Complex, so that the Complex can be reborn as a sustainable innovation ecosystem.

• Korean Journal of Environmental Biology
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• v.21 no.2
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• pp.194-202
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• 2003

To isolate alga-lytic bacteria, a number of samples were collected from Lake of Sukchon and Pal'tang reservoir where cyanobacteria blooming occurred. HY0210-AK1, which exhibited high alga-lytic activity, was isolated using Anabaena cylindrica lawn. The morphological and biochemical characteristics of the isolate HY0210-AK1 were very similar to that of the genus Rhizobium. Taxonomic identification including 16S rDNA base sequencing and phylogenetic analysis indicated that the isolate Hy0210-AK1 had a 99.1% homology in its 16S rDNA babe sequence with Sphingobium herbicidovorans. A. cylindrica NIES-19 was susceptible to the alga-lytic bacterial attack. The growth-inhibiting offset of the bacterium was not different on A. cylindrica NIES-19 when Sphingobium herbicidovorans HY0210-AK1 was in the lag, exponential, and stationary growth phase, although the alga-Iytic effect of S. herbici-dovorans HY0210-AK1 that in stationary growth phase was somewhat pronounced at the first time of inoculation. When S. herbicidovorans HY0210-AK1 was inoculated was inoculated with $1\times 10^{8}$ CFU $ml^{-1}$ together with A cylindrica NIES-19, the bacterium proliferated and caused algal lysis. A. cylindrica NIES-19 died when S. herbicidovorans HY0210 AKl was added to the algal culture but not when duly the filtrates from the bacterial culture was added. This suggests that extracellular substances are not responsible for inhibition of A. cylindrica NIES-19 and that algal Iysis largely attributed to direct interaction between S. herbicidovorans HY0210-AK1 and A. cylindrica NIES-19. The alga-lytic bacterium HY0210-AK1 caused cell lysis and death of three strain of Micro-cystis aeruginosa, but revealed no alga-Iytic effects on the Stephanodiscus hantzschii.