• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.79-79
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• 2003

세포에 대한 산화스트레스는 세포의 대사와 기능저하의 원인이 되고 있으며 이를 줄이기 위한 항산화 물질의 첨가가 연구되고 있다. 본 연구는 비특이면역증가제이며 다목적 고기능성 알칼리용액 조성물인 Barodon의 항산화 효과를 돼지정자를 이용하여 조사하여, 돼지 액상정액의 보존성 향상을 위한 Barodon의 이용성을 알기 위하여 시도하였다. 실험구로서 무첨가구와 활성산소 인위발생구(xanthine+xanthine oxidase, X-XO), X-XO구에 superoxide dismutase, cataiase, Barodon(2종류)의 단독처리구 및 X-XO구에 이들 항산산제의 복합처리구로 나누어 항산화제 처리효과에 따른 정자운동특성을 CASA로 분석하였다. 또한 돼지 액상정액에서 Barodon의 항산화 효과를 무첨가구, catalase구 및 Barodon구로 나누어 정액의 보존기간별 정액성상 변화(정자활력, 생존성, 첨체이상)를 조사하였다.

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.27-27
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• 2002

본 연구는 돼지 인공수정 시 1 회 주입하는 정자농도가 번식성적에 미치는 영향을 구명하기 위하여 활력이 70%이상인 정자수 기준으로 80㎖ 병당 30.0×10/sup 8/80㎖, 25.0, 20.0, 17.5, 15.0, 12.5, 10.0 및 7.5로 농도를 각각 조절하여 12시간 간격으로 2회 인공수정하고 분만율 및 산자수를 조사하였다. 본 실험에 사용된 액상정액은 축산기술연구소 돼지 인공수정 센타의 종모돈중 통일품종 정액을 BTS(Beltsville thawing solution) 보존액으로 혼합하여 5개 농장의 암돼지에 인공수정 하였으며, 정자농도는 광전비색계(Spectronic-20, USA)를 이용하여 측정하였다. 분만율은 72.3%-89.3%로 농도별로 통계적인 유의차는 인정되지 않았다. (중략)

• Korean Journal of Animal Reproduction
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• v.23 no.2
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• pp.113-118
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• 1999

This study was carried out to investigate on the improvement of fertilizing ability of in vitro matured oocytes from sperm density and motility by intracytoplasmic sperm injection(ICSI) into the porcine oocytes. 1. The in vitro fertilization and cleavage rates of oocytes from 1.0, 2.0, 3.0, 5.0 ($\times$10$^{6}$ $m\ell$) sperm concentration by IVF and ICSI of porcine oocytes were 46.7%~75.0%, 60.0%~85.7% and 10.6%~25.0%, 20.0%~64.3%, respectively. 2. The in vitro fertilization and cleavage rates of oocytes from 20, 40, 60, 80% of sperm mortilty by IVF and ICSI of porcine oocytes were 46.4%~71.4%, 67.9%~85.7% and 7.1%~21.4%, 28.6%~60.7%, respectively. 3. The in vitro fertilization and developmental rates of oocytes by IVF and ICSI methods were 55.6%~60.0%, 77.8%~80.0% and 17.8%~24.0%, 42.2%~56.0%, respectively. This ICSI method was improved high fertilization rates of porcine oocytes.

• Korean Journal of Animal Reproduction
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• v.23 no.2
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• pp.165-174
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• 1999

Boar semen can be frozen successfully. However, there is a large variability in the extent of damage boar semen samples experiences during cryopreservation. This experiment was undertaken to find out factors that affect a post-thaw viability of boar spermatozoa. For this purpose, cryodiluents(BF5, LEY, Soejima and M-Soejima), cryoprotectants(glycerol. ethylene glycol, and propylene glycol), pre-freezing method(dryice-pellet, dryice-straw and L$N_2$vapour-st-raw) and total time required for freezing(2. 5, and 7 h) were compared as a factors. To investigate quality of semen during freezing process, motility(%), normal apical ridges(%, NAR), and proportion of living sperm(%) by flow cytometic analysis were assessed after collection, cooled, pre-frozen and post-thawing. Post-thaw motility of semen diluted with M-Soejima was 52.0%, respectively. When heparin, caffeine or heparin＋caffeine was added to 2nd cryodiluent of M-Soejima during freezing process, the highest motility after thawing was shown at the addition of caffeine (2mM), with 61.7$\pm$2.9% of motility. M-Soejima with heparin or caffeine was significantly higher than that of controI(p＜0.05). The result using glycerol(Gly), ethylene glycol(EG), propylene glycol(PG), and their mixture (Gly＋EG and Gly＋PG) as cryoprotectants, the highest motility was shown at the mixture treatment with Gly plus PG. However, the highest proportion of live spermatozoa was shown at Gly＋EG, there was no significantly difference among treatments(p＞0.05). When semen was pre-frozen with three manners(dryice-pellet, dryice-straw, and L$N_2$ vapor-straw), motility(%) of post-thaw spermatozoa was the highest in the L$N_2$ vapor-straw pre-freezing method of M-Soejima cryodiluent with 57.5% of motility, For a simple, economical and timesaving approach to freezing boar semen, total time required for freezing were 2, 5, and 7 hours, post-thaw motility were 43.8, 45.0 and 38.8%, NAR were 19.5, 22.7 and 28.5%, and viability were 20.8, 19.9 and 22.1%, respectively. This data suggests that boar semen diluted with M-Soejima cryodiluent contained caffeine, using mixture of glycerol and propylene glycol or ethylene glycol as cryoprotectants, frozen with 2 hours, can be taken better motility, NAR, and proportion of live spermatozoa.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2004.10a
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• pp.61-61
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• 2004

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.10
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• pp.181-186
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• 2020

This study examined the artificial fertilization efficiency of crossbred goats from a farmhouse using frozen semen. Electrostimulation was used to ejaculate and collect semen to assess the artificial fertilization efficiency of crossbred goats. The sperm concentration, vitality, and vitality after melting were investigated. The sperm volume was within 2.5~3 ml, and the concentration was 21~25 × 10⁸/ml for each male crossbred goat. The melted semen had high vitality (≥90%). An IDEXX Rapid Visual Pregnancy Test kit was used for an earlier diagnosis of the pregnancy and to determine the pregnancy rate of fertilization using frozen-thawed semen. The reproductive performance of the artificially fertilized crossbred goats had the highest delivery rate (68%) from Farm C and the lowest delivery rate (45%) from farm A. The delivery rate through artificial fertilization was equal to the fertilization rate according to early pregnancy diagnostic kits. The artificial insemination efficiency was 45~68%. These findings can be used as the basis for improvement and breeding goats in goat farms and livestock research institutes.

• Korean Journal of Animal Reproduction
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• v.24 no.3
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• pp.231-239
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• 2000

In this study, we observed the effects of dioxin on weights of body, testes and other organs, the number and motility of sperm in the a various dose after two days' of administration in mice. Animals were treated with oral dose of dioxin 10, 20, 30, 40 mg/kg body weight, respectively. 1, After administration dioxin at doses of 10, 20, 30 and 40 $\mu\textrm{g}$/kg to the mice, the changes in body weights were 30.6 $\pm$ 2.g~40.7 $\pm$ 3.9g and 30.8 $\pm$4.1g~39.5g $\pm$3.1 for 10 and 20 $\mu\textrm{g}$/kg dosed group, 31.0 $\pm$ 3.5g ~ 39.0 $\pm$ 3.5g, 30.6 $\pm$ 3.4g~38.3 $\pm$ 4.0g for 30 and 40 $\mu\textrm{g}$/kg dosed group. The body weight of dioxin-administered group showed lower value when compared to 30.6 $\pm$ 2.8g ~ 44.5 $\pm$ 3.1g of which is control group's. 2. After administration of dioxin at doses of 10, 20, 30 and 40 $\mu\textrm{g}$/kg to the mice, the increase in the number of WBC was prominent, but the increase in the number of RBC wasn't significant, though the values of Hb, PCV, and PLT were higher than those of control group's. 3. After administration of dioxin at doses of 10. 20, 30 and 40 $\mu\textrm{g}$/kg to the mice, the changes in sperm number were 112.5 $\pm$ 3.7~119.4 $\pm$4.2 $\times$ 10$^{6}$ $m\ell$, 103.9 $\pm$3.8 ~ 110.2 $\pm$ 3.6 $\times$ 10$^{6}$ $m\ell$, 97.5 $\pm$ 3.4 ~105.7 $\pm$ 4.4 $\times$ 10$^{6}$ $m\ell$, 87.2 $\pm$ 3.7~98.5 $\pm$ 3.8 $\times$ 10$^{6}$ $m\ell$, respectively. The sperm number of dioxin-administered group showed lower value than that of control group's, which was 119.0 $\pm$ 4.3 ~ 120.7 $\pm$ 4.8 $\times$ 10$^{6}$ $m\ell$. After administration of dioxin at doses of 10~40 $\mu\textrm{g}$/kg to the mice, the sperm motility were 69.4$\pm$ 3.0 ~ 86.6 $\pm$4.7%. The sperm motility of dioxin-administered group showed lower value than that of control group's. 4. After administration of dioxin at doses of 10, 20, 30 and 40$\mu\textrm{g}$/kg to the mice, the organ weight of each dioxin-administered group's was decreased a little compared to that of control group's. 5. After administration of dioxin at doses of 10, 20, 30 and 40 $\mu\textrm{g}$/kg to the mice, the weights of spleen, kidneys, and liver showed increase a little.

• Korean Journal of Poultry Science
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• v.42 no.2
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• pp.109-116
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• 2015

Ogye rooster sperm chromatin status can be detected using well established sperm assays. In this paper, a simple and fast method to monitor rooster sperm chromatin status could be employed in field for assessment of chicken sperm quality. Using standard bright field microscope, Diff-Quik stains can be reproducibly, easily and routinely monitored with simple staining. The presence of abnormal chromatin staining of rooster sperm was determined by darker stain in head. In the fresh semen, the viabilities of three tested Ogye spermatozoa were 93.53%, 82.42% and 90.63% and normal chromatin rates were 87.96%, 74.25% and 85.10% respectively. However, after freezing, the rates of viability of thawed semen were reduced to 69.58%, 61.98% and 72.20% and normal chromatin rate also reduced to 58.91%, 48.49% and 63.34%. A significant correlation between live sperm and normal sperm nuclei was 0.875 in fresh semen and 0.513 in frozen semen. After incubation of sperm at $37^{\circ}C$ for 5min, the rates of viability, chromatin normality and sperm head activity were shown as $90.63{\pm}1.28%$, $82.44{\pm}8.09%$ and $66.68{\pm}10.29%$ in fresh semen. However, the rates of thawed semen were reduced to $67.92{\pm}7.55%$, $56.92{\pm}12.15%$ and 47.32{\pm}5.02%, respectively. The relationship between chromatin normality and sperm head movements in fresh and thawed semen were 0.564 and 0.540, respectively. With these results, the chicken sperm normality could be assessed by the Diff-Quik staining that could be used for chromatin status of sperm head and activated morphology of live spermatozoa, as a simple and rapid staining method.

• Journal of Embryo Transfer
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• v.18 no.1
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• pp.43-50
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• 2003

The objective of this study was to investigate the effect of cryopreservation by slow and rapid freezing on the sperm motility index, viability and morphology of post-thaw human spermatozoa. After rapid freezing and thawing, sperm motility index was significantly higher (MOT:47.40$\pm$20.06%, VCL : 38.12$\pm$15.58 $\mu$m/s, VSL : 28.19$\pm$14.10 $\mu$m/s, VAP:33.64$\pm$15.15 $\mu$m/s, and HYP 2.77$\pm$2.71%) than slow freezing and thawing(MOT : 43.39$\pm$ 18.79%, VCL .33.91 $\pm$ 13.50 Um/s, VSL . 19.98$\pm$0.88 $\mu$m/s, VAP : 24.60$\pm$11.72 $\mu$m/s, and HYP . 1.33$\pm$1.57% ; P<0.05). But sperm Linearity(LIN) was significantly lower(28.83 $\pm$ 10.35) comparing to the slow freezing method(34.64 $\pm$ 11.36 ; P<0.05). On the other hand, significant difference were not observed MAD, WOB, DNC and DNM by slow and rapid frozen-thawed methods. After rapid freezing and thawing, sperm viability was lower(60 $\pm$ 2.2%) than slow freezing method(62 $\pm$2.1%) and sperm morphology was higher(46$\pm$7.7%) than that(44: 8.3). But there was no significantly These results indicate that rapid freezing method was positive effect of sperm cryopreservation in human.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.77-77
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• 2001

본 연구는 돼지 동결정액의 번식성적을 개선코자 기존의 maxi-straw와 cryogenic-vial을 이용하여 포장방법에 따른 동결방법과 융해방법별 정액성상 및 번식성적을 비교하였다. 동결방법은 두 가지 포장방법 모두 정액의 양(5$m\ell$)과 농도(5.0$\times$$10^{9}$/dose)가 동일한 조건으로 처리하였으며, LYE(Lactose egg york extender) 보존액으로 희석하여 액체질소 상단 15cm에서 20분간 동결하였다. 융해방법은 maxi-straw는 52$^{\circ}C$에서 45초간 cryogenic-vial은 52$^{\circ}C$에서 190초간 융해하여 $25^{\circ}C$로 가온 된 80$m\ell$ BTS (Beltsville thawing solution) 보존액과 혼합하였다. 정액성상검사는 정자자동분석기(SAIS : Sperm Analysis Image System, Korea)를 이용하였다. 총활력(TM : Total motility)과 정자의 빠르기(VCL : Curve linear velocity)는 maxi-straw가 54.3%와 46.6%로 cryogenic-vial의 35.6%와 36.6%보다 우수하였다(P<0.05). 정자의 직진성(STR : Straightness)과 NAR은 maxi-straw가 53.2%와 32.6%로 cryogenic-vial의 47.3%와 29.8%와 비슷한 경향을 나타내었다. 수태율과 분만율 및 총산자수는 maxi-straw가 77.3%, 68.2% 및 8.0두로 조사되어 cryogenic-vial포장방법의 66.7%, 61.9% 및 7.4두보다 다소 우수하였으나 통계적인 유의차는 인정되지 않았다. 이상의 결과로 볼 때 cryogenic-vial방법이 새로운 돼지 동결정액 포장방법의 가능성을 나타낸다고 사료된다.