Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Artificial Insemination and Delivery Rate of Crossbred Goat using Frozen-Thawed Semen

동결정액을 활용한 교잡종 염소의 인공수정 효율 및 분만율 조사

  • Kim, Kwan-Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Eun-Do (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Jinwook (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Dong-Kyo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Sung-Soo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Sang-Hoon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
  • 김관우 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이은도 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이진욱 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김동교 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이성수 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이상훈 (농촌진흥청 국립축산과학원 가축유전자원센터)
  • Received : 2020.09.07
  • Accepted : 2020.10.05
  • Published : 2020.10.31
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Abstract

This study examined the artificial fertilization efficiency of crossbred goats from a farmhouse using frozen semen. Electrostimulation was used to ejaculate and collect semen to assess the artificial fertilization efficiency of crossbred goats. The sperm concentration, vitality, and vitality after melting were investigated. The sperm volume was within 2.5~3 ml, and the concentration was 21~25 × 108/ml for each male crossbred goat. The melted semen had high vitality (≥90%). An IDEXX Rapid Visual Pregnancy Test kit was used for an earlier diagnosis of the pregnancy and to determine the pregnancy rate of fertilization using frozen-thawed semen. The reproductive performance of the artificially fertilized crossbred goats had the highest delivery rate (68%) from Farm C and the lowest delivery rate (45%) from farm A. The delivery rate through artificial fertilization was equal to the fertilization rate according to early pregnancy diagnostic kits. The artificial insemination efficiency was 45~68%. These findings can be used as the basis for improvement and breeding goats in goat farms and livestock research institutes.

본 연구에서는 동결정액을 활용한 농가에서 사육중인 교잡종 염소의 인공수정 효율을 조사하고자 실시하였다. 교잡종 염소들의 인공수정을 위하여 전기자극을 주어 정액을 채취한 후 동결정액을 조제하고 정자농도, 정자활력, 융해 후 정자의 활력을 조사하였다. 그 결과 교잡종 염소 수컷의 개통별 정액채취량은 2.5~3 ml 내외였고 정액의 농도는 21~25 × 108/ml로 나타났다. 동결된 정액을 융해 하였을 때 정자의 활력도 90% 이상으로 높게 나타났다. 동결정액을 활용하여 교잡종 염소 사육 3 농가에서 인공수정을 실시한 후 임신진단 키트(IDEXX Rapid Visual Pregnancy Test kit)로 임신유무를 조기에 진단한 결과 각 농가별 인공수정 후 수태률은 A농장 45%, B농장 50%, C농장 68%로 조사되었다. 또한 인공수정 후 약 150일 후 출산한 교잡종 염소들의 번식성적을 조사한 결과 C농장이 68%로 분만율이 가장 높게 조사 되었고 A농장이 45%로 가장 낮게 조사되었다. 일반적으로 인공수정 후 수태율과 분만율은 약간의 차이가 나타나는 경향이 있는데 본 실험에서는 인공수정 후 임신을 조기에 진단한 결과와 분만된 개체의 분만율이 동일하게 조사되었다. 본 연구의 결과 동결정액을 활용한 교잡종 염소의 인공수정시 농가별 효율의 차이는 있으나 인공수정 효율이 45~68% 범위로 조사되었다. 본 연구결과는 일반 염소 사육 농가뿐만 아니라 축산연구기관에서 염소의 개량과 번식을 위한 기초 자료로 활용 될 수 있을 것이다.

Keywords

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