• Journal of Embryo Transfer
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• v.14 no.2
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• pp.107-111
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• 1999

The present study was performed to evaluate the best electric fusion condition in nuclear transfer, Korean Native Cattle fibroblasts were used as nucleic donors. Oocytes from slaughterhouse were matured in vitro for 22 h and enucleated. Each individual cells were transferred into enucleated ocytes and reconstructed embryo were placed into the fusion chamber. In experiment 1, pulse were performed by altering pulse duration at 1. 75kv/cm, 1 time. When pulse duration is 30$mutextrm{s}$, fusion and development rates is higher than other conditions. In experiment 2, the effect of different pulse number were studied at the pulse duration 30$mutextrm{s}$ and the same pulse intensity. When pulse number was one, fusion rates were higher than other conditions. The fused embryos were moved to culture medium and assessed their development to blastocyst. These results showed that best fusion condition was 30$mutextrm{s}$ and one time. And the fibroblasts derived from Han Woo can be reprogrammed by nuclear transplantation and develop subsequently in vitro.

• Journal of Embryo Transfer
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• v.14 no.3
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• pp.171-176
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• 1999

This study was carried out to investigate the effect of co-culture system(bovine oviduct epithelial cells; BOEC) and defined culture system (modified TALP ; mTALP) on the conception of embryos transferred, and pregnancy and twin birth rates after transfer of fresh or frozen-thawed bovine blastocysts produced in vitro were also evaluated. Oocytes from the slaughterhouse ovaries were matured and fertilized using general protocol. The results obtained were as the following. The pregnancy rate after transfer was higher in co-culture group than in mTALP group, but was not signficantly different, and there is no difference between fresh embryo group and frozen-thawed embryo group in conception rate. The conception rate was not different whether 3∼4 blastocysts or 2 blatocysts transferred into a recipient, but the production rate of twin calves was significantly higher (p<0.05) when 3∼4 embryos transferred. The average birth weight of twin calves(24.38kg) was numerically, but not significantly lighter than that of single calves(26.68kg).

• Journal of Embryo Transfer
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• v.10 no.1
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• pp.91-100
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• 1995

3.3kb 웅성특이 DNA(pEM39 plasmid DNA)가 성 특이 DNA 검색자로 활용되어질 수 있는가를 확인하기 위하여 구조적인 분석을 Southern blotting, DNA sequencing과 computer program 분석을 통하여 실시하였다. 전체 3.3kb에서 유래된 약 1kb 단위의 단편을 이용하여 표지된 짧은 DNA probe들은 Southern blot 분석에서 웅성특이성을 나타내었다. McGraw와 Jeon의 sequence에 대한 유사성 비교 자료로부터 여러 부분의 conserved region을 찾아내고 이것을 기초로 하여 5개의 primer set들을 선발하였다. Conserved region에 존재하면서 computer program에 의해서 선발되어진 PMS1과 2의 primer set가 최종적으로 PCR 분석을 위하여 선정되었다. 이 primer set를 사용한 PCR 분석에서, 1ng부터 10pg까지의 웅성 genomic DNA에서 PCR 산물을 얻을 수 있었으며, 자성의 경우는 어떠한 산물도 찾을 수 없었다. PCR에 이용할 수정란의 시료는 2 세포기의 수정란에서 얻었으며 순수 분리된 genomic DNA에서 확립된 조건에서 PCR을 수행하였다. 8개의 수정란을 분석한 결과 4개의 웅성과 4개의 자성 수정란을 확인하였다. 이러한 결과는 선정된 primer set가 돼지 수정란의 성을 조기 감별하는데 효율적인 DNA probe로 사용될 수 있다는 것을 암시한다.

• Journal of Embryo Transfer
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• v.11 no.3
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• pp.317-321
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• 1996

본 연구는 수정능획득유기물질로 알려진 카페인 헤파린을 병행처리하여 한우 정자의 첨체 반응율과 생존율을 알아보고 수정능획득과정 중에 단백질의 변화상을 전기영동방법으로 조사하였다. 동결융해후 정자의 생존율은 90%이상이었으나 전배양처리후 0.5시간에 70%로 감소하고 2시간 이후에는 35%로 감소하였다. 정자의 첨체반응율은 동결융해후 정상정자가 85.7%였으나 전배양시간에 따라 53.4%에서 14.3%로 감소하였다. 동결융해후 첨체가 소실된 생존정자는 9.4%였으나 전배양시간에 따라 17.2%에서 46.8%로 증가하였다. 원정액에서는 분자량 20,000정도에서 동결융해후 전자보다 특이적으로 많은 단백질량을 나타내었으나 동결융해 후 정자를 0.5에서 2.0시간으로 전배양하였을 때 전배양시간에 따른 단백질상의 변화는 대조구와 큰 차이가 없었다.

• Journal of Embryo Transfer
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• v.11 no.1
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• pp.81-83
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• 1996

This study was carried out to investigate the effect of sperm concentration of 5ml maxi-straw on farrowing rate and number of pigs born alive per litter in deep frozen boar semen. We did not find out the effect of sperm concentration on post-thaw sperm motility and NAR acrosome. However, farrowing rate and number of pigs born alive per litter of 7. 5 x 10˚ /5ml and 10.0 x 10˚ /5m1 sperm concentrations were higher than those of 5. 0 /10˚ /5ml sperm concentration.

• Journal of Embryo Transfer
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• v.9 no.1
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• pp.73-83
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• 1994

The objective of this study was to develop an effective in vitro production system capable of obtaining more porcine embryos from immature oocytes. These experiments were thus conducted to examine the effect of oocytes type and maturation time on the in vitro maturation(IVM) and fertilization(IVF) of oocytes and the in vitro development (IVD)of IVF embryos. 1. The degree of oocyte maturation based on cumulus expansion index(GEI) did not differ for A- and B-typed oocytes but the index of oocyte type C was lower(P<0.05) than that of other oocyte types. 2. When the oocytes of type A and B were matured for 36, 42 and 48hrs, the GEl was not different between the 36- and 42-h maturation but the GEl after 48hrs was greatly lower(P<0.05) than that of other maturation times. 3. The highest cleavage rate(48.6%) of IVF oocytes was obtained from A typed oocytes and 42-h maturation but the developmental potential based on cleavage index was the highest when B-typed oocytes were matured for 42hrs.

• Journal of Embryo Transfer
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• v.9 no.1
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• pp.111-116
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• 1994

This study was carried out to investigate the aggregation rate of isolated mouse 2-, 4- and 8-cell stage blastomeres in phytohemagglutinin(PHA) solution. The morphologically normal embryos were collected from the oviduct of superovulated female mouse by flushing with M2 and the zona pellucida of embryos were removed with 0.5% pronase. The blastomeres were isolated by pipetting after plunging into Ca++-Mg++free PBS for 20 min. The result showed that aggregation rate in 0.5% (84.9~93.1%) was higher than that in 1.0% PHA(76.0~82.1%). Optimal aggregation time was 60min (83.9~100.0%) when compared with 30min (78.8~87.5%). Developmental to blastocyst in recombinated blastomeres was higher under conditions of 0.5% PHA solution and 60-min aggregation than that under other conditions.

• Proceedings of the Korean Information Science Society Conference
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• 1999.10a
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• pp.629-631
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• 1999

최근의 분산처리 기술은 이질적인 분산환경에서의 상호 운용성을 보장하는 시스템통합 기술을 중심으로 발전해 나가고 있다. 이러한 환경을 제공하는 대표적 분산 미들웨어인 OMG CORBA는 현재 많은 산업체와 연구기관에서 구현하여 상용화하거나 연구용으로 제공하고 있다. 그러나 하나의 제품에서 개발된 CORBA 응용 프로그램을 다른 제품에서 이식에 필요한 코드 수정 없이 바로 사용할 수 없는 문제점을 보이고 있어 OMG에서 CORBA를 통해 성취하려고 하는 중요한 목표인 이식성과 상호운용성을 보장하지 못하고 있다. 본 논문은 Java 언어 매핑을 지원하는 ORB를 대상으로 위 문제의 해결에 필요한 CORBA 규격 준수 시험을 수행하는 ORB 기능 시험에 대하여 기술한다.

• The Journal of Korean Academy of Prosthodontics
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• v.48 no.4
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• pp.259-265
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• 2010

Purpose: This study was aimed to evaluate the effect of different sizes of $\beta$-TCP/ HA particles on vertical bone augmentation using titanium mesh in the cranium of rabbits. Materials and methods: Six white rabbits weighing 5kg were used. Four circular grooves of 6mm diameter were made by trephine, and five small holes were drilled in the inner surface of each circular gooves. Different sizes of grafts (small 0.3 - 0.5 mm, medium 0.5 - 1.0, large 1.0 - 2.0 mm) were placed respectively in the experimental groups. Titanium mesh (height 3 mm, width 6 mm) was placed. After 8weeks healing period, the rabbits were euthanized, and the specimens were prepared for histological findings. New bone formation and remaining graft area were measured to calculate the ratio of areas occupying the inner space of titanium mesh. Mann-Whitney U-test and Wilcoxon signed rank-test were used for statistical analysis ($\alpha$ = .05). Results: The experimental groups with $\beta$-TCP/HA graft showed a significantly higher new bone formation (P = .003). Comparing different sizes of $\beta$-TCP/HA, there was no statistical difference in terms of new bone formation. The vertical bone formation (i.e. new bone and graft area) was significantly greater in $\beta$-TCP/HA groups (P = .001). In comparison between different sizes of $\beta$-TCP/HA, medium size group had significantly greater area than large particle size group (P = .039). Conclusion: The use of $\beta$-TCP/HA with titanium mesh showed a higher vertical bone formation, particularly the medium sized $\beta$-TCP/HA particles (0.5 - 1.0 mm) produced better results in vertical bone augmentation.

• Korean Journal of Animal Reproduction
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• v.20 no.4
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• pp.467-472
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• 1997

This study was conducted to determine the optimal DC voltage for NT of in vivo donor embryo nuclei and investigate the effect of donor embryo guality on fusion and in vitro development of NT embryos . Recpient oocytes were enucleated 25~27h after IVM and further cuitured for 18~20h prior to fusion for oocyte aging. Donor embryos of molura stage were recovered from superovulated heifers and classified l into good and low quality group. Their nuclei were transferred in to the emucleated oocytes 42~44h post-IVM and fused 43-45h post-IVM with a single 0.75kV /cm or 1.0kV /cm DC voltage for 70${\mu}\textrm{A}$sec. The fusion rate of oocytes was not different between two DC voltages. However, the cleavage and M + B developmnent was more high at 1.0kV /cm DC voltage and the proportion of M+B was 19.0% at 0.75kV /cm DC and 29.4% at 1.0kV /cm DC voltage. Donor embryo qualtiy did not greatly affect the fusion and cleavage of NT oocytes, but none of NT embryos derived from low embryo quatity reached the morula stage. The results indicate that the most suitable DC v voltage for electrofusion of in vivo donor muclei was a single 1.0kV /cm DC voltage and donor embryo quality was an important factor affecting the development in vitro of NT embryos.