Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Production of HanWoo(Koran Native Cattle) Twin Calves by Transfer of Bovine Blastocysts Produced In Vitro

체외생산 소 배반포의 이식에 의한 한우 쌍태 생산

  • 윤종택 (한경대학교 동물생명자원학과) ;
  • 이호준 (한경대학교 동물생명자원학과) ;
  • 노상호 (한경대학교 동물생명자원학과) ;
  • 정연길 (한경대학교 동물생명자원학과) ;
  • 손동수 (농촌진흥청 축산기술연구소) ;
  • 김일화 (농촌진흥청 축산기술연구소) ;
  • 류일선 (농촌진흥청 축산기술연구소) ;
  • 김창근 (중앙대학교 축산학과) ;
  • 정영채 (중앙대학교 축산학과)
  • Published : 1999.12.01
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Abstract

This study was carried out to investigate the effect of co-culture system(bovine oviduct epithelial cells; BOEC) and defined culture system (modified TALP ; mTALP) on the conception of embryos transferred, and pregnancy and twin birth rates after transfer of fresh or frozen-thawed bovine blastocysts produced in vitro were also evaluated. Oocytes from the slaughterhouse ovaries were matured and fertilized using general protocol. The results obtained were as the following. The pregnancy rate after transfer was higher in co-culture group than in mTALP group, but was not signficantly different, and there is no difference between fresh embryo group and frozen-thawed embryo group in conception rate. The conception rate was not different whether 3∼4 blastocysts or 2 blatocysts transferred into a recipient, but the production rate of twin calves was significantly higher (p<0.05) when 3∼4 embryos transferred. The average birth weight of twin calves(24.38kg) was numerically, but not significantly lighter than that of single calves(26.68kg).

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