• Title/Summary/Keyword: 정밀공학

Search Result 7,099, Processing Time 0.031 seconds

Validation of Stem-loop RT-qPCR Method on the Pharmacokinetic Analysis of siRNA Therapeutics (Stem-loop RT-qPCR 분석법을 이용한 siRNA 치료제의 생체시료 분석법 검증 및 약물 동태학적 분석)

  • Kim, Hye Jeong;Kim, Taek Min;Kim, Hong Joong;Jung, Hun Soon;Lee, Seung Ho
    • Journal of Life Science
    • /
    • v.29 no.6
    • /
    • pp.653-661
    • /
    • 2019
  • The first small interfering RNA (siRNA) therapeutics have recently been approved by the Food and Drug Administration in the U.S., and the demand for a new RNA therapeutics bioanalysis method-which is essential for pharmacokinetics, including the absorption, distribution, metabolism, and excretion of siRNA therapeutics-is rapidly increasing. The stem-loop real-time qPCR (RT-qPCR) assay is a useful molecular technique for the identification and quantification of small RNA (e.g., micro RNA and siRNA) and can be applied for the bioanalysis of siRNA therapeutics. When the anti-HPV E6/E7 siRNA therapeutic was used in preclinical trials, the established stem-loop RT-qPCR assay was validated. The limit of detection was sensitive up to 10 fM and the lower limit of quantification up to 100 fM. In fact, the reliability of the established test method was further validated in three intra assays. Here, the correlation coefficient of $R^2$>0.99, the slope of -3.10 ~ -3.40, and the recovery rate within ${\pm}20%$ of the siRNA standard curve confirm its excellent robustness. Finally, the circulation profiles of siRNAs were demonstrated in rat serum, and the pharmacokinetic properties of the anti-HPV E6/E7 siRNA therapeutic were characterized using a stem-loop RT-qPCR assay. Therefore, the stemloop RT-qPCR assay enables accurate, precise, and sensitive siRNA duplex quantification and is suitable for the quantification of small RNA therapeutics using small volumes of biological samples.

Anomaly Detection for User Action with Generative Adversarial Networks (적대적 생성 모델을 활용한 사용자 행위 이상 탐지 방법)

  • Choi, Nam woong;Kim, Wooju
    • Journal of Intelligence and Information Systems
    • /
    • v.25 no.3
    • /
    • pp.43-62
    • /
    • 2019
  • At one time, the anomaly detection sector dominated the method of determining whether there was an abnormality based on the statistics derived from specific data. This methodology was possible because the dimension of the data was simple in the past, so the classical statistical method could work effectively. However, as the characteristics of data have changed complexly in the era of big data, it has become more difficult to accurately analyze and predict the data that occurs throughout the industry in the conventional way. Therefore, SVM and Decision Tree based supervised learning algorithms were used. However, there is peculiarity that supervised learning based model can only accurately predict the test data, when the number of classes is equal to the number of normal classes and most of the data generated in the industry has unbalanced data class. Therefore, the predicted results are not always valid when supervised learning model is applied. In order to overcome these drawbacks, many studies now use the unsupervised learning-based model that is not influenced by class distribution, such as autoencoder or generative adversarial networks. In this paper, we propose a method to detect anomalies using generative adversarial networks. AnoGAN, introduced in the study of Thomas et al (2017), is a classification model that performs abnormal detection of medical images. It was composed of a Convolution Neural Net and was used in the field of detection. On the other hand, sequencing data abnormality detection using generative adversarial network is a lack of research papers compared to image data. Of course, in Li et al (2018), a study by Li et al (LSTM), a type of recurrent neural network, has proposed a model to classify the abnormities of numerical sequence data, but it has not been used for categorical sequence data, as well as feature matching method applied by salans et al.(2016). So it suggests that there are a number of studies to be tried on in the ideal classification of sequence data through a generative adversarial Network. In order to learn the sequence data, the structure of the generative adversarial networks is composed of LSTM, and the 2 stacked-LSTM of the generator is composed of 32-dim hidden unit layers and 64-dim hidden unit layers. The LSTM of the discriminator consists of 64-dim hidden unit layer were used. In the process of deriving abnormal scores from existing paper of Anomaly Detection for Sequence data, entropy values of probability of actual data are used in the process of deriving abnormal scores. but in this paper, as mentioned earlier, abnormal scores have been derived by using feature matching techniques. In addition, the process of optimizing latent variables was designed with LSTM to improve model performance. The modified form of generative adversarial model was more accurate in all experiments than the autoencoder in terms of precision and was approximately 7% higher in accuracy. In terms of Robustness, Generative adversarial networks also performed better than autoencoder. Because generative adversarial networks can learn data distribution from real categorical sequence data, Unaffected by a single normal data. But autoencoder is not. Result of Robustness test showed that he accuracy of the autocoder was 92%, the accuracy of the hostile neural network was 96%, and in terms of sensitivity, the autocoder was 40% and the hostile neural network was 51%. In this paper, experiments have also been conducted to show how much performance changes due to differences in the optimization structure of potential variables. As a result, the level of 1% was improved in terms of sensitivity. These results suggest that it presented a new perspective on optimizing latent variable that were relatively insignificant.

Development and Performance Evaluation of Multi-sensor Module for Use in Disaster Sites of Mobile Robot (조사로봇의 재난현장 활용을 위한 다중센서모듈 개발 및 성능평가에 관한 연구)

  • Jung, Yonghan;Hong, Junwooh;Han, Soohee;Shin, Dongyoon;Lim, Eontaek;Kim, Seongsam
    • Korean Journal of Remote Sensing
    • /
    • v.38 no.6_3
    • /
    • pp.1827-1836
    • /
    • 2022
  • Disasters that occur unexpectedly are difficult to predict. In addition, the scale and damage are increasing compared to the past. Sometimes one disaster can develop into another disaster. Among the four stages of disaster management, search and rescue are carried out in the response stage when an emergency occurs. Therefore, personnel such as firefighters who are put into the scene are put in at a lot of risk. In this respect, in the initial response process at the disaster site, robots are a technology with high potential to reduce damage to human life and property. In addition, Light Detection And Ranging (LiDAR) can acquire a relatively wide range of 3D information using a laser. Due to its high accuracy and precision, it is a very useful sensor when considering the characteristics of a disaster site. Therefore, in this study, development and experiments were conducted so that the robot could perform real-time monitoring at the disaster site. Multi-sensor module was developed by combining LiDAR, Inertial Measurement Unit (IMU) sensor, and computing board. Then, this module was mounted on the robot, and a customized Simultaneous Localization and Mapping (SLAM) algorithm was developed. A method for stably mounting a multi-sensor module to a robot to maintain optimal accuracy at disaster sites was studied. And to check the performance of the module, SLAM was tested inside the disaster building, and various SLAM algorithms and distance comparisons were performed. As a result, PackSLAM developed in this study showed lower error compared to other algorithms, showing the possibility of application in disaster sites. In the future, in order to further enhance usability at disaster sites, various experiments will be conducted by establishing a rough terrain environment with many obstacles.

Observation of Ice Gradient in Cheonji, Baekdu Mountain Using Modified U-Net from Landsat -5/-7/-8 Images (Landsat 위성 영상으로부터 Modified U-Net을 이용한 백두산 천지 얼음변화도 관측)

  • Lee, Eu-Ru;Lee, Ha-Seong;Park, Sun-Cheon;Jung, Hyung-Sup
    • Korean Journal of Remote Sensing
    • /
    • v.38 no.6_2
    • /
    • pp.1691-1707
    • /
    • 2022
  • Cheonji Lake, the caldera of Baekdu Mountain, located on the border of the Korean Peninsula and China, alternates between melting and freezing seasonally. There is a magma chamber beneath Cheonji, and variations in the magma chamber cause volcanic antecedents such as changes in the temperature and water pressure of hot spring water. Consequently, there is an abnormal region in Cheonji where ice melts quicker than in other areas, freezes late even during the freezing period, and has a high-temperature water surface. The abnormal area is a discharge region for hot spring water, and its ice gradient may be used to monitor volcanic activity. However, due to geographical, political and spatial issues, periodic observation of abnormal regions of Cheonji is limited. In this study, the degree of ice change in the optimal region was quantified using a Landsat -5/-7/-8 optical satellite image and a Modified U-Net regression model. From January 22, 1985 to December 8, 2020, the Visible and Near Infrared (VNIR) band of 83 Landsat images including anomalous regions was utilized. Using the relative spectral reflectance of water and ice in the VNIR band, unique data were generated for quantitative ice variability monitoring. To preserve as much information as possible from the visible and near-infrared bands, ice gradient was noticed by applying it to U-Net with two encoders, achieving good prediction accuracy with a Root Mean Square Error (RMSE) of 140 and a correlation value of 0.9968. Since the ice change value can be seen with high precision from Landsat images using Modified U-Net in the future may be utilized as one of the methods to monitor Baekdu Mountain's volcanic activity, and a more specific volcano monitoring system can be built.

Evaluation of Road and Traffic Information Use Efficiency on Changes in LDM-based Electronic Horizon through Microscopic Simulation Model (미시적 교통 시뮬레이션을 활용한 LDM 기반 도로·교통정보 활성화 구간 변화에 따른 정보 이용 효율성 평가)

  • Kim, Hoe Kyoung;Chung, Younshik;Park, Jaehyung
    • KSCE Journal of Civil and Environmental Engineering Research
    • /
    • v.43 no.2
    • /
    • pp.231-238
    • /
    • 2023
  • Since there is a limit to the physically visible horizon that sensors for autonomous driving can perceive, complementary utilization of digital map data such as a Local Dynamic Map (LDM) along the probable route of an Autonomous Vehicle (AV) is proposed for safe and efficient driving. Although the amount of digital map data may be insignificant compared to the amount of information collected from the sensors of an AV, efficient management of map data is inevitable for the efficient information processing of AVs. The objective of this study is to analyze the efficiency of information use and information processing time of AV according to the expansion of the active section of LDM-based static road and traffic information. To carry out this objective, a microscopic simulator model, VISSIM and VISSIM COM, was employed, and an area of about 9 km × 13 km was selected in the Busan Metropolitan Area, which includes heterogeneous traffic flows (i.e., uninterrupted and interrupted flows) as well as various road geometries. In addition, the LDM information used in AVs refers to the real high-definition map (HDM) built on the basis of ISO 22726-1. As a result of the analysis, as the electronic horizon area increases, while short links are intensively recognized on interrupted urban roads and the sum of link lengths increases as well, the number of recognized links is relatively small on uninterrupted traffic road but the sum of link lengths is large due to a small number of long links. Therefore, this study showed that an efficient range of electronic horizon for HDM data collection, processing, and management are set as 600 m on interrupted urban roads considering the 12 links corresponding to three downstream intersections and 700 m on uninterrupted traffic road associated with the 10 km sum of link lengths, respectively.

Assessing forest net primary productivity based on a process-based model: Focusing on pine and oak forest stands in South and North Korea (과정기반 모형을 활용한 산림의 순일차생산성 평가: 남북한 소나무 및 참나무 임분을 중심으로)

  • Cholho Song;Hyun-Ah Choi;Jiwon Son;Youngjin Ko;Stephan A. Pietsch;Woo-Kyun Lee
    • Korean Journal of Environmental Biology
    • /
    • v.41 no.4
    • /
    • pp.400-412
    • /
    • 2023
  • In this study, the biogeochemistry management (BGC-MAN) model was applied to North and South Korea pine and oak forest stands to evaluate the Net Primary Productivity (NPP), an indicator of forest ecosystem productivity. For meteorological information, historical records and East Asian climate scenario data of Shared Socioeconomic Pathways (SSPs) were used. For vegetation information, pine (Pinus densiflora) and oak(Quercus spp.) forest stands were selected at the Gwangneung and Seolmacheon in South Korea and Sariwon, Sohung, Haeju, Jongju, and Wonsan, which are known to have tree nurseries in North Korea. Among the biophysical information, we used the elevation model for topographic data such as longitude, altitude, and slope direction, and the global soil database for soil data. For management factors, we considered the destruction of forests in North and South Korea due to the Korean War in 1950 and the subsequent reforestation process. The overall mean value of simulated NPP from 1991 to 2100 was 5.17 Mg C ha-1, with a range of 3.30-8.19 Mg C ha-1. In addition, increased variability in climate scenarios resulted in variations in forest productivity, with a notable decline in the growth of pine forests. The applicability of the BGC-MAN model to the Korean Peninsula was examined at a time when the ecosystem process-based models were becoming increasingly important due to climate change. In this study, the data on the effects of climate change disturbances on forest ecosystems that was analyzed was limited; therefore, future modeling methods should be improved to simulate more precise ecosystem changes across the Korean Peninsula through process-based models.

The Study on the Confidence Building for Evaluation Methods of a Fracture System and Its Hydraulic Conductivity (단열체계 및 수리전도도의 해석신뢰도 향상을 위한 평가방법 연구)

  • Cho Sung-Il;Kim Chun-Soo;Bae Dae-Seok;Kim Kyung-Su;Song Moo-Young
    • The Journal of Engineering Geology
    • /
    • v.15 no.2 s.42
    • /
    • pp.213-227
    • /
    • 2005
  • This study aims to assess the problems with investigation method and to suggest the complementary solutions by comparing the predicted data from surface investigation with the outcome data from underground cavern. In the study area, one(NE-1) of 6 fracture zones predicted during the surface investigation was only confirmed in underground caverns. Therefore, it is necessary to improve the confidence level for prediction. In this study, the fracture classification criteria was quantitatively suggested on the basis of the BHTV images of NE-1 fracture zone. The major orientation of background fractures in rock mass was changed at the depth of the storage cavern, the length and intensity were decreased. These characteristics result in the deviation of predieted predicted fracture properties and generate the investigation bias depending on the bore hole directions and investigated scales. The evaluation of hydraulic connectivity in the surface investigation stage needs to be analyze by the groundwater pressures and hydrochemical properties from the monitoring bore hole(s) equipped with a double completion or multi-packer system during the test bore hole is pumping or injecting. The hydraulic conductivities in geometric mean measured in the underground caverns are 2-3 times lower than those from the surface and furthermore the horizontal hydraulic conductivity in geometric mean is six times lower than the vertical one. To improve confidence level of the hydraulic conductivity, the orientation of test hole should be considered during the analysis of the hydraulic conductivity and the methodology of hydro-testing and interpretation should be based on the characteristics of rock mass and investigation purposes.

Application of Molecular Biological Technique for Development of Stability Indicator in Uncontrolled Landfill (불량매립지 안정화 지표 개발을 위한 분자생물학적 기술의 적용)

  • Park, Hyun-A;Han, Ji-Sun;Kim, Chang-Gyun;Lee, Jin-Young
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.28 no.2
    • /
    • pp.128-136
    • /
    • 2006
  • This study was conducted for developing the stability parameter in uncontrolled landfill by using a biomolecular investigation on the microbial community growing through leachate plume. Landfill J(which is in Cheonan) and landfill T(which is in Wonju) were chosen for this study among a total of 244 closed uncontrolled landfills. It addressed the genetic diversity of the microbial community in the leachate by 165 rDNA gene cloning using PCR and compared quantitative analysis of denitrifiers and methanotrophs with the conventional water quality parameters. From the BLAST search, genes of 47.6% in landfill J, and 32.5% in landfill T, respectively, showed more than 97% of the similarity where Proteobacteria phylum was most significantly observed. It showed that the numbers of denitrification genes, i.e. nirS gene and cnorB gene in the J site are 7 and 4 times higher than those in T site, which is well reflecting from a difference of site closure showing 7 and 13 years after being closed, respectively. In addition, the quantitative analysis on methane formation gene showed that J1 spot immediately bordering with the sources has the greatest number of methane formation bacteria, and it was decreased rapidly according to distribute toward the outer boundary of landfill. The comparative investigation between the number of genes, i.e. nirS gene, cnorB gene and MCR gene, md the conventional monitoring parameters, i.e. TOC, $NH_3-N,\;NO_3-N,\;NO_2-N,\;Cl^-$, alkalinity, addressed that more than 99% of the correlation was observed except for the $NO_3-N$. It was concluded that biomolecular investigation was well consistent with the conventional monitoring parameters to interpret their influences and stability made by leachate plume formed in downgradient around the uncontrolled sites.

Tensile Performance of Machine-Cut Dovetail Joint with Larch Glulam (낙엽송집성재를 이용한 기계프리커트 주먹장접합부의 인장성능)

  • Park, Joo-Saeng;Hwang, Kweon-Hwan;Park, Moon-Jae;Shim, Kug-Bo
    • Journal of the Korean Wood Science and Technology
    • /
    • v.38 no.3
    • /
    • pp.199-204
    • /
    • 2010
  • Members used for the Korean traditional joints have been processed by handicraft, especially with domestic red pine species. Dovetail joint is most commonly used in woodworking joinery and traditional horizontal and vertical connections. It is able to be processed much easier to cut by handicraft and machines. However, although it is processed straight forwards, it requires a high degree of accuracy to ensure a snug fit. Also, tenons and mortises must fit together with no gap between them so that the joint interlocks tightly. A few scientific studies on the dovetail joints have been conducted so far. For the effective applications of traditional joints and domestic plantation wood species, dovetail joints were assembled by larch glulam members processed by machine pre-cut. To identify the tensile properties of through dovetail joints, larch glulam with 150 150mm in cross section were prepared. Furthermore, various geometric parameters of dovetai joints such as width, length, and tenon angle, were surveyed. The ends in the mortise was cracked mainly at a low strength level in the control specimens without reinforcements. The maximum tensile strengths of reinforced specimens considering real connections such as capital joint and headpiece on a column, increasedby handicraft, especially with domestic red pine species. Dovetail joint is most commonly used in woodworking joinery and traditional horizontal and vertical connections. It is able to be processed much easier to cut by handicraft and machines. However, although it is processed straight forwards, it requires a high degree of accuracy to ensure a snug fit. Also, tenons and mortises must fit together with no gap between them so that the joint interlocks tightly. A few scientific studies on the dovetail joints have been conducted so far. For the effective applications of traditional joints and domestic plantation wood species, dovetail joints were assembled by larch glulam members processed by machine pre-cut. To identify the tensile properties of through dovetail joints, larch glulam with 150 150mm in cross section were prepared. Furthermore, various geometric parameters of dovetai joints such as width, length, and tenon angle, were surveyed. The ends in the mortise was cracked mainly at a low strength level in the control specimens without reinforcements. The maximum tensile strengths of reinforced specimens considering real connections such as capital joint and headpiece on a column, increased by two times with shear failures on the tenon than the control specimens. The maximum tensile strength was obtained in the specimen of 25 degrees, and no difference was observed in the changes of neck widths.

Live Load Distribution in Prestressed Concrete I-Girder Bridges (I형 프리스트레스트 콘크리트 거더교의 활하중 분배)

  • Lee, Hwan-Woo;Kim, Kwang-Yang
    • Journal of the Computational Structural Engineering Institute of Korea
    • /
    • v.21 no.4
    • /
    • pp.325-334
    • /
    • 2008
  • The standard prestressed concrete I-girder bridge (PSC I-girder bridge) is one of the most prevalent types for small and medium bridges in Korea. When determining the member forces in a section to assess the safety of girder in this type of bridge, the general practice is to use the simplified practical equations or the live load distribution factors proposed in design standards rather than the precise analysis through the finite element method or so. Meanwhile, the live load distribution factors currently used in Korean design practice are just a reflection of overseas research results or design standards without alterations. Therefore, it is necessary to develop an equation of the live load distribution factors fit for the design conditions of Korea, considering the standardized section of standard PSC I-girder bridges and the design strength of concrete. In this study, to develop an equation of the live load distribution factors, a parametric analysis and sensitivity analysis were carried out on the parameters such as width of bridge, span length, girder spacing, width of traffic lane, etc. As a result, the major variables to determine the size of distribution factors were girder spacing, overhang length and span length in case of external girders. For internal adjacent girders, the determinant factors were girder spacing, overhang length, span length and width of bridge. For internal girders, the factors were girder spacing, width of bridge and span length. Then, an equation of live load distribution factors was developed through the multiple linear regression analysis on the results of parametric analysis. When the actual practice engineers design a bridge with the equation of live load distribution factors developed here, they will determine the design of member forces ensuring the appropriate safety rate more easily. Moreover, in the preliminary design, this model is expected to save much time for the repetitive design to improve the structural efficiency of PSC I-girder bridges.