• Journal of Life Science
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• v.33 no.2
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• pp.199-207
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• 2023

To prepare for the threat of a future epidemic in the post-COVID-19 era, research based on the one-health concept (i.e., the health of humans, animals, and the environment as "one") is essential. Cross-species infections are being identified as a result of the high infection rate and viral load of SARS-CoV-2 in humans. The possibility of transmission of SARS-CoV-2 from humans to mink has been determined. In addition, the transmission of SARS-CoV-2 from humans to cats through contact has been considered possible. The data so far show that livestock and poultry are less likely to be infected with SARS-CoV-2. However, if infections are established through a new mutation, the resulting diseases are expected to have enormous ripple effects on various fields, such as human food security, the economy, and trade. In addition, there are concerns about the endemic prospect of SARS-CoV-2 and the high accessibility of companion animals. This is because the evolution of the virus likely occurs in animal hosts. Once SARS-CoV-2 is established in other species, they might serve as intermediate hosts for the re-emergence of the virus in the human population. Thus, it is necessary to ensure a rapid response to future outbreaks by accumulating research data on the animal infection of SARS-CoV-2. These data can have implications for the development of animal models for vaccines and therapeutics against SARS-CoV-2. Therefore, in this study, epidemiological reviews were analyzed, and response strategies against SARS-CoV-2 infection in animals were presented using the One-health approach.

• Parasites, Hosts and Diseases
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• v.28 no.1
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• pp.39-44
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• 1990

The life history of Echinostoma cinetorchis was completed in the laboratory using Hippeutis cantors as the first and second intermediate host. The incubation for maturation of eggs and hatching of miracidia took 24 days at 28℃. On the 66th day after miracidial challenge, 16 snails were crushed and examined for the presence of E. cinetorchis larvae. The metacercariae were detected in all of the snails, and from three of them were found the rediae and cercariae. The morphological characteristics of the larvae and the experimentally obtained adults were compatible with that of E. cinetorchis.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.12 no.1
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• pp.88-92
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• 2009

Although transfusion of blood and plasma products are accepted as the principle means of transmission of HCV, other parenteral methods, such as acupuncture, tattooing needles, piercing, and surgery are possible methods of transmission of HCV. We managed a case of chronic hepatitis C acquired through ear piercing and acupuncture. A 10-year old girl presented with nausea, abdominal pain, and anorexia for 1 month. Her laboratory finding showed the following: AST/ALT, 865/1,290 IU/L; positive anti-HCV Ab; and HCV RNA. One year previously, she was treated with acupuncture for an ankle sprain and 2 years previously, she had her ears pierced. Laboratory findings of family members showed AST/ALT in the normal ranges, and negative anti-HCV Ab and HCV RNA. The pathologic findings of a liver biopsy revealed chronic hepatitis with mild lobular activity, moderate porto-periportal activity, and portal fibrosis. She was treated with pegylated interferon ${\alpha}$-2a and oral ribavirin for 6 months, after which the clinical symptoms and laboratory findings improved.

• Journal of Veterinary Clinics
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• v.23 no.1
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• pp.22-30
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• 2006

This study was carried out to assess the usefulness of thermography for diagnosis and healing process of musculoskeletal disorder in horses. Horses with experimentally induced infectious arthritis were treated with bee venom or antibiotics for 5 weeks from 24 hours after inoculation of Staphylococcus aureus, the effectiveness of the 2 therapeutic drugs treatment was similar to each other. Thermographic evaluation was similar to any other tests, because the thermal pattern tend to return to normal with declining similar to those of clinical signs, laboratory experiments and healing of the lesion. This ability to assess inflammatory change noninvasively make thermography an ideal imaging tool to aid in the diagnosis of certain lameness condition or musculoskeletal disease in horses. These results suggested that thermographic evaluation in horses with musculoskeletal disorders were useful for diagnosis and helpful for monitoring of healing process.

• Korean Chemical Engineering Research
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• v.44 no.1
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• pp.40-45
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• 2006

The baculovirus-insect Drosophila melanogaster S2 cell system combines advantages of conventional baculovirus system and non-lytic S2 cell system because baculoviruses can infect non-permissive cells such as mammalian and Drosophila S2 cells but cannot replicate themselves inside the cells. In the present work, we investigated effects of infection conditions on production of green fluorescent protein (GFP) as a target protein using this baculovirus-S2 cell system. Even though higher MOI and longer baculovirus contact time showed better GFP expression yield during the shorter period, overall protein yield could be lower during the longer period due to the relatively higher cell detachment and lysis (lower cell viability). In addition, maintaining high MOI will be not practical for large-scale cell culture. Therefore, instead of maintaining high MOI, we found that high initial cell number and concentrated (10X) baculovirus volume can confer comparable protein expression even under the moderate MOI condition. Also, we found that the post-infection time that is connected to state of cells after infection was an important factor for production yield.

• Korean Journal of Clinical Laboratory Science
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• v.55 no.3
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• pp.143-150
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• 2023

The global outbreak of COVID-19 has underscored the pressing need for robust infection control practices in pulmonary function laboratories (PFL). However, the existing guidelines and regulatory frameworks provided by relevant authorities in the country have revealed certain deficiencies in effectively addressing this significant public health crisis. This study surveyed the infection control regulations, disposable item usage, ventilation facilities, spatial separation, and the configuration of entrance doors in 51 domestic hospital facilities from Oct 1, 2021, to Nov 2, 2021. The survey findings revealed that while there was a relatively satisfactory adherence to airborne, droplet, and contact precautions with adequate awareness and utilization of personal protective equipment, the environmental disinfection practices exhibited a suboptimal performance rate of 39.22% per patient. Depending on the specific survey domains, substantial variations were observed in the utilization of disposable items (81.05%), ventilation systems (45.75%), dedicated testing spaces (80.39%), separation of administrative areas (15.69%), and the installation of automated doors (19.61%). This study not only highlights the paramount importance of infection control in PFLs within domestic medical institutions but also provides foundational data for developing and enhancing standardized guidelines that align with international benchmarks for infection control in these settings.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2020.07a
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• pp.630-633
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• 2020

무의식적인 손-얼굴의 접촉으로 인한 감염의 문제점을 해결하기 위해, 얼굴 만지기 행동을 인식할 필요가 있다. 본 연구는 최근 각광을 받는 딥러닝 기술을 이용하여 비디오 영상에서 얼굴 만지기 행동 인식에 대한 연구이다. 우선, 비디오 영상에서 얼굴 만지기와 관련된 11 가지 행동에 대한 시, 공간적 특징을 컨볼루션 신경망을 통해 추출한다. 추출된 정보는 각 행동 레이블로 인코딩되어 비디오 영상에서 얼굴 만지기 행동을 분류한다. 또한, 3D, 2D 컨볼루션 신경망의 대표 네트워크인 I3D, MobileNet v3에 대해 비교 실험을 진행한다. 제안하는 시스템을 적용하여 인간의 행동을 분류하는 실험을 진행했을 때, 얼굴을 만지는 행동을 99%의 확률로 구분했다. 이 시스템을 이용하여 일반인이 무의식적인 얼굴 만지기 행동에 대해서 정량적으로 또는 적시적으로 인식을 하여, 안전한 위생 습관을 확립하여 감염의 확산방지에 도움을 줄수 있기를 바란다.

• Journal of Life Science
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• v.21 no.2
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• pp.227-234
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• 2011

The hrp gene cluster in the plant pathogen Pseudomonas syringae is a key determinant of pathogenicity. Recent studies have demonstrated that specific host cell induction of the Ralstonia solanacearum hrp gene cluster is controlled by the PrhA (plant regulator of hrp) receptor. To characterize the role that P. syringae PrhA plays in the virulence of plant cells, a prhA homolog was isolated from P. syringae pv. tabaci and a $\Delta$prhA mutant was constructed by allelic exchange. The $\Delta$prhA mutant had reduced virulence in the host plant, and co-culture of P. syringae pv. tabaci and plant cell suspensions induced a much higher level of hrpA gene transcription than culture in hrp-inducing minimal medium. These results indicate that PrhA of P. syringae is a putative pathogen-plant cell contact sensor, therefore, we used a hrpA-gfp reporter fusion to monitor the in situ expression of PrhA. The results of this study demonstrated that PrhA induces hrp gene expression in P. syringae pv. tabaci in the presence of plant cells.

• Parasites, Hosts and Diseases
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• v.36 no.2
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• pp.91-98
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• 1998

The present study was performed to observe characteristics of the lifE history of Echinoparvphium recunpatum under both natural and laboratory conditions in Korea. A batch of Rcnix aunculnria corecna was collected from Sunamchon, one of the stream of West Naktonggang (River), in Kangso-gu, Pusan during August and September 1992. Out of 106 snails examined by crushing,52 (49.0%) were infected with larval E. recurvaum i.e. rediae, cercariae and metacercariae. Cercariae naturally shed from snails encysted in the snails of same species and loaches, but not in mud-snails. Adult worms were detected from chicks and ducks experimentally infected with metacercariae, but not from rats and mice. The average recovery rate of adults from chicks was 13.1%. Rediae were sac-like, $2.437{\;}{\times}{\;}0.317{\;}mm$ in average size, with a muscular pharynx and a brownish cecum which reached the anterior half of the body. Cercariae consisted of a spindle-shaped body ($0.262{\;}{\times}{\;}0.129{\;}mm$ in average) and a rod-like tail ($0.528{\;}{\times}{\;}0.056{\;}mm$ in average). In the cercarial body, 45 collar spines were observed on the head crown, and double rows of excretory ducts with fine granules were laterally arranged between the pharynnx and the ventral sucker. Metacercariae were spherical, $0.144{\;}{\times}{\;}0.142{\;}mm in average size, with thick hyaline outer and thin elastic inner walls, and many excretory granules. Adults were slender and more attenuated in the anterior end, $0.2760{\;}{\times}{\;}0.550{\;}mm$ in average size, and had 45 collar spines including four end group spines on both ventral corners. From the above results, it was confirmed that R. auriculnna corennc plays a pivotal role in the life cycle of 5. recuwatum as the first and/or second intermediate hosts in Korea.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.1
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• pp.264-273
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• 2014

The aim of the study is to investigate the awareness and practice of Standard Precautions towards Hospital Infection Control in Emergency medical technology students. Data were collected from 289 questionnaires from March 20, 2012 to April 25, 2012. The data were analyzed using SPSS 18.0 for window(SPSS Inc, USA) with the level of significance as 0.05. According to the results, significant differences were shown in gender, infection control education and infection control educational institutions. The factors influencing the practice of standard precautions were found to be the times of the contact to blood, body fluid, mucus, or the skin with lesions and the cognition of standard precautions. Cognition level of the EMT students was high but the practice level was low. Based on the results of this study, it is proposed to develop an effective educational program to improve the cognition and practice, and to improve the cognition and practice of the standard precaution through the systematic and repetitive education and training.