• KSBB Journal
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• v.14 no.4
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• pp.503-510
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• 1999

In order to analyze nutritional factors affecting in situ bioremediation of diesel degradation and cell viability were studied by varying nutritional conditions. In column experiments packed with diesel-contaminated soil, nitrogen was found to be the major limiting nutrient. When nitrogen was added to soil at four different levels of C : N (100 : 5, 100 : 10, 100 : 15, and 100 : 20 mg N/kg dry soil), the greatest simulation of microbial activity occurred at the lowest, rather than the highest nitrogen addition. However, no significant effects was observed when phosphorus and air were added. No matter how the incubation mode varied, less than 50% of the diesel was remained after 7 days of treatment, presumably because the residual hydrocarbons were adsorbed on soil particles, adsorption

• Korean Journal of Soil Science and Fertilizer
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• v.8 no.1
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• pp.37-43
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• 1975

Effect of inoculation trial on plant height and dry matter yield of seven alfalfa varieties (Medicago sativa L. var. Washoe, Ranger, Lahontan, Narragensett, Atlantic, Vernal, and Moapa 69) which are considered to be well adapted in these areas were conducted during 1973-1974 in a relatively poor soil at the field station in Kyung Hee University. Root nodule bacteria: Rhizobium meliloti (Noculator Northrup Kings Co. Ltd) was applied at a rate to provide 6 oz/30l of alfalfa seeds. Seven varieties were seeded in pots on May 31, 1973 and in second year, they were transplanted in field. 1. Dry matter yield and plant height of all inoculation plots were significantly greater than those of the no inoculation plots in all varieties. 2. In the 1973, dry matter yield of inoculation plot a in pot experiment was in the order of Moapa 69> Vernal> Lahontan> Washoe> Atlantic> Narragensett> Ranger. 3. There was no difference in dry matter yield between with and without-inoculation after transplanted in a field in the second year (1974). Total dry matter yield of varieties was in the order of Washoe> Lahontan> Moapa 69> Ranger> Narragensett> Vernal> Atlantic.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.1
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• pp.85-88
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• 1987

Quercus acutissima seedlings were grown for two years in fumigated or non-fumigated in nursery soil in a medium with and without vegetative inoculum of the ectomycorrhizal fungi, Pisolitlius tinctorius (Pt) and Rhizopogon rubescens (Rr). Mycorrhizal formation were 42% in fumigated Pt inoculation and 36% in fumigated Rr inoculation. $2.16{\ell}\;per\;m^2$ of Pt vegetative inoculum in fumigated soil stimulated the seedling height (98%), root collar diameter (132%) and weight (420%). And Rr inoculation in fumigated soil increased the seedling height (44%), root collar diameter (23%) and weight (157%) compared with non-treated plot. There was no effect of Pt and Rr inoculation on the growth in non-fumigated soil. Nitrogen and $P_2O_5$ contents in foliage were not different by the treatment but $K_2O$ and Ca in fumigated soil were higher than non-fumigated soil.

• Korean journal of applied entomology
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• v.40 no.1
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• pp.83-88
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• 2001

Residual effect of imidacloprid on brown planthopper (BPH) and residues on rice plant were investigated in pot and field tests. Residual effect (showing 100% mortality) of imidacloprid of granule formulation on the BPH lasted for 40 days at a recommended dose (0.3 kg a.i./ha) and for 30 and 20 days at a half and a quarter of the recommended dose, respectively. In case of liquid formulation of imidacloprid, the residual effect lasted for 40 days at both recommended dose (0.032 kg a.i.1ha) and a half of it, and lasted for 30 days at a quarter of it. The next generation formation of BPH was throughly depressed by the imidacloprid application at the former generation. This result supports the residual effect of imidacloprid on BPH. Seed dressing (3 ghg) showed insecticidal activity (96.7% mortality) for 55 days after treatment. When granule formulation of imidacloprid was applied to soil at a recommended dose , imidacloprid residue in leaves of rice plant increased up to 0.46 ppd for 6 days and then decreased to 0.09 ppm on 40 days after application. Residue of imidacloprid in leaves of rice plant increased up to about 0.23 ppm and about 0.2 ppm for 6 days when applied at a half and a quarter of the recommended dose, respectively and after that they decreased.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.2
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• pp.198-206
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• 1981

A field research was conducted to evaluate the effects of inoculation of Rhizobium meliloti and lime application on the early growth of alfalfa, var. Luna, in an acid soil. Alfalfa seeds were inoculated by lime-peat pelleting method with $A_3$ strain isolated from alfalfa roots. Both inoculated and noninoculated seeds were sown in acid soil (pH 5.5) with three levels of lime application (1, 3, 6 ton/ha) on mid-April. Inoculated seeds produced many effective nodules in early stage and increased nitrogen and chlorophyll contents of leaf, consequently stimulating alfalfa growth. Pseudo nodules were found in noninoculated, while they were seldom formed in inoculated. Lime application increased soil pH, plant height, root length, and shoot and root weight. Liming effect on nodulation was significant at first cut, but no longer after then. Both inoculation and liming increased the forage yield of alfalfa. Inoculation and liming of 6 ton/ha together produced the highest yield. As compared to control plot of nitrogen application, the effect of seed inoculation only could be equal to about 120 kg/ha nitrogen application. The total nitrogen contents of forage were little affected by number or weight of nodules and amounts of lime application.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2007.05a
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• pp.339-345
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• 2007

이번 연구에서는 pH 조작이 어려운 소규모 하수 처리장에서의 BNR 슬러지의 접종으로 인한 SBR start-up시 영양 염류 제거 특성 및 변화를 관찰 하였다. 1) 글루코즈를 탄소원으로 사용한 유기물 제거에 있어서 모두 85%이상의 높은 제거 효율을 보였으나 BNR 슬러지 접종 비율이나 유무에 따른 SBR의 유기물 제거에 미치는 영향은 미비한 것으로 나타났다. 2) 유입비율에 따라 암모니아 제거 효율은 BNR 슬러지 첨가 유무에 따라 바뀌었으며, 비율에 상관없이 첨가된 반응조는 7일 이내 $82{\sim}98%$에 달하는 제거율을 보였다. 3) 인 제거효율에 있어서는 유입비율에 따라 인 제거 효율이 증가하였으며, 첨가와 미첨가의 차이는 25%이상의 인 방출량 차이를 나타내었다. 4) 첨가한 BNR 슬러지의 비율이 전체의 40%이상일 때는 50%일 때와 큰 차이를 보이지 못했다. 5) FISH를 통하여 미생물 군집을 확인하였고 각 미생물들 간의 상대적인 비율과 heterotroph의 급격한 증식을 확인할 수 있었다. 6) pH, DO, ORP graph를 통한 Reactor의 실시간 조정 가능성을 볼 수 있었다. 7) BNR 슬러지를 접종하지 않을 경우, PAOs와 질산화 미생물의 성장이 더뎌 영양염류 제거가 어려웠다.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.235-238
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• 2000

The production of polysaccharide from Paecilomyces japonica was studied in the shake flask culture. For the cell growth and the polysaccharide production, the optimum synthetic medium was glucose peptone(YMP) medium. The flask culture conditions for the polysaccharide production were $27^{\circ}C$ and 200rpm with the initial pH 9 for 8days cultivation.

• Korean Journal of Veterinary Research
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• v.36 no.4
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• pp.895-902
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• 1996

산란계에 불활화 개 파보바이러스 백신을 근육내로 1주 간격으로 4회 접종하여 면역화시키고 최종 접종 2주후에 채란하여 $4^{\circ}C$에 보관하며 사용하였다. 난황항체는 5% HPMCP를 이용하여 분리하였고 0.5% HPMCP 용액은 lipid 침전에 매우 효과적이었으며 희석배수 10배에서 투명한 상층액을 나타내었다. 1차분리한 상층액의 단백질 농도는 2.5mg/ml이었고 최종 단백질 용액의 경우는 26.53mg/ml이었다. SDS-PAGE 전기영동상에서 분자량 60~70 KD 및 30~40 KD의 2 band가 나타났으며 non-reducing 전기영동에서는 닭 혈청 IgG와 같은 120~160 KD의 분자량을 보인 band가 각각의 분리용액에서 나타났다. 난황 항체의 개 파보바이러스에 대한 혈구응집억제반응 항체역가는 혈청의 역가에 비해 1주의 차이를 주며 증가했으며 난황 항체는 1:640에서 1:2560, 혈청은 1:640에서 1:5120을 나타내었다.

• The Korean Journal of Mycology
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• v.30 no.1
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• pp.11-17
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• 2002

This experiment was carried out to study formation of fruitbody with Cordyceps scarabaeicola (EFCC C-252) isolate. This isolate was the one of best fruitbody formation on brown rice 60 g plus 30 g silkworm pupa media among EFCC C-251, EFCC C-252, EFCC C-1092 from EFCC (Entomopathogenic Fungal Culture Collection) of Kangwon National University. Fruiting body was formed only isolate EFCC C-252 among tested isolates on the medium of brown rice (60 g) and silkworm pupae (30 g). The optimal temperature and light for fruiting body formation were $25^{\circ}C$ and fluorescent light (300 lux). The maximal fruiting body formation was observed at 70 g of brown rice and 80 g of silkworm pupa medium which was treated separately. Fruiting body was formed maximally by 2 days interval of irrigation.

• Microbiology and Biotechnology Letters
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• v.30 no.3
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• pp.253-257
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• 2002

The time-dependent changes of red pigments production in solid-state plant scale fermentor using barley cultured with Monascus sp., instead of rice which was traditionally used, were investigated in this study. A steady increase in the yield of red pigments in barley occurred between the 3rd and 6th days. The optimized conditions (inoculation volume = 6∼8%, initial pH = 6, air supply = 0.6∼0.8 m) promoted the production of red pigments. Short-time steaming of barley (< 20 min) decreased fungal growth and pigments production due to the insufficient gelatiniza-tion. The optical density of the red pigments under the optimized conditions was 120 at 500 ]nm per gram of barley. In addition, the metabolites from the fermented barley with Monucus sp. showed antibacterial effects against Escherichia coli and Salmonella typhimurium. Barley was shown to be one of the best grain sources for solid-state fermentation with Monascu sp., fur obtaining natural pigments and also functional food materials.