• Food Science and Preservation
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• v.13 no.1
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• pp.77-82
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• 2006

This study was carried out to investigate the Cheongbukjang fermentation characteristics and to select the strain showing a fibrinolytic activity. Fibrinolytic activity of 5 strains isolated from the commercial Chungkukjang was tested N2 strain showed the highest activity $(41.7\%)$ while N3 and N5 had similar activity $(27.8\%)$ compared to plasmin 1 unit/mL The selected N2 strain was determined as B. subtilis with $90.1\%$ homology by API kit analysis. Quality characteristics of Cheongbukjang fermented by 6 kinds of strains were tested Among 3 strains cultured, B. subtilis (KCTC 3014) showed the highest viscous substance, fibrinolytic activity and amino type nitrogen content. After isolated, B. subtilis N2 showed the highest viscous substance, fibrinolytic activity and amino type nitrogen content. Optimum steam-time for Cheongbukjang fermented by B. subtilis (KCTC 3014) and B. subtilis N2 was 45 min while optimum fermentation-time was 20 hr.

• Korean Journal of Food Science and Technology
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• v.37 no.2
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• pp.255-260
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• 2005

To make Chungkuk-jang with enhanced fibrinolytic and immunostimulating activities, 220 strains isolated from Chungkuk-jangs were tested, and 13 Bacillus strains with excellent proteolytic and polysaccharide-producing activities were selected and tested for their fibrinolytic and immunostimulating activities using fibrin plate method and RAW 264.7 cell line, respectively. To assess macrophage activation, contents of cytokines such as tumor necrosis factor ($TNF-{\alpha}$) and $interleukin-1{\alpha}$ and nitric oxide were measured. Three strains showing highest fibrinolytic and immunostimulating activities were identified as Bacillus licheniformis (CHKJ 1249, 1326) and Bacillus subtilis (CHKJ 1339).

• Horticultural Science & Technology
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• v.31 no.1
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• pp.110-116
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• 2013

This study was carried out to establish the simple mass-screening methods for resistant tomato to Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL). Root dip inoculation method has been used in many studies on the resistance of tomato to disease. On the other hand, in mass-screening for resistant tomato to Fusarium wilt, the inoculation method is time-consuming and laborious procedure. Disease development of two FOL isolates on two cultivars of tomato according to inoculation method including root dip, tip and scalpel methods were investigated. In compatible interaction, tomato seedlings of each cultivar inoculated by tip method showed the lower and more variable disease severities than by root dip method. Whereas the seedlings by scalpel method represented clear resistant and susceptible responses to Fusarium wilt as root dip method. The resistance degree of each cultivar inoculated with FOL isolates by scalpel method was hardly affected by the tested incubation temperature and inoculum concentration. On the basis of the results, we suggest scalpel inoculation method as an efficient mass-screening method for resistant of tomato cultivars to Fusarium wilt. Roots of tomato seedlings at two-leaf stage grown in plastic cell tray were injured with scalpel and then spore suspension (more than $1{\times}10^7\;conidia{\cdot}mL^{-1}$) of FOL was poured directly on the roots. The infected plants were cultivated in a growth room at $25-30^{\circ}C$ for 4 weeks with 12-hours light a day.

• Weed & Turfgrass Science
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• v.4 no.4
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• pp.348-359
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• 2015

Total 443 isolates of actinomycetes were isolated from turfgrass rhizosphere as potential biological control agents. The two isolates (S11 and S4) showed highest cellulase activity with compared to the other isolates that exhibited a clear zone of 1.2 mm around the colony on cellulose agar medium. S12 strain appeared the most active chitin degrading, which exhibited a 1.2 mm of clear zone. The highest proteolytic activity on skim milk agar was which exhibited a 7.5 mm of clear zone by S2 strain. S1 strain from the soli showed siderophore production ability, which exhibited a 0.6 mm of large clear zone on chrome azurol S agar. The antifungal activity of the volatile compound producing by 4 selected actinomycetes was investigated that inhibition rate against Rhizoctonia solani AG2-2 and Sclerotinia homoeocarpa. Growth inhibition effect of S8 isolate against S. homoeocarpa was appeared to 94.8%, S2 to 76.9%, S5 to 46.1% and S12 to 43.5%. The significant inhibition effects on mycelial growth of S. homoeocarpa were shown on media with four strains. The inhibition effect was the highest with S8 strain treatment at 94.8%.

• Pediatric Infection and Vaccine
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• v.9 no.2
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• pp.208-214
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• 2002

Purpose : The number of newborns vaccinated with BCG of Tokyo 172 strain, which has been claimed to cause lesser degree of local adverse events including scar, has recently been increasing. However, tuberculin response to this vaccine has inadequately been studied, especially with newborns cared at primary care clinics. We, therefore, performed a study in newborns vaccinated with BCG at private pediatric offices and evaluated the response to PPD 2TU or PPD 5TU following vaccination with percutaneous or intradermal BCG. Methods : Two hundred infants who had been cared at three private pediatric offices were retrospectively enrolled in the study. One hundred fifty one infants had received percutaneous BCG(Tokyo strain); 129 infants had had tuberuclin test with PPD 2TU and the rest of 22 infants with PPD 5TU. Forty nine infants had received intradermal BCG(28 infants Copenhagen strain, I infant French strain, 20 infants unknown); 35 infants had had tuberculin test with PPD 2TU, 14 infants(11%) with PPD 5TU. Results : In infants vaccinated with percutaneous BCG, the mean induration diameter in tuberculin test was significantly greater with PPD 5TU($12.4{\pm}3.5mm$) compared to PPD 2TU ($9.2{\pm}4.4mm$). In infants vaccinated with intradermal BCG, the mean induration diameters in tuberculin test were $5.7{\pm}5.1mm$ to PPD 2TU and $6.6{\pm}4.8mm$ to PPD 5TU, which were not significantly different. The tuberculin response to PPD 2TU was significantly greater in infants vaccinated with percutaneous BCG compared to those with intradermal BCG. The tuberculin response to PPD 5TU was also significantly greater in infants vaccinated with percutaneous BCG compared to those with intradermal BCG. Conclusion : Percutaneous BCG(Tokyo strain) seems to cause greater response to tuberculin compared to intradermal BCG and PPD 2TU induces weaker response compared to PPD 5TU. Acknowledging some discrepancies from the previously reported data, which might have been due to the different source of the study subjects, more studies are needed to establish the range of tuberculin response following BCG vaccination in order to differentiate from tuberculosis.

• Korean Journal of Food Science and Technology
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• v.35 no.1
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• pp.111-114
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• 2003

Conjugated linoleic acid (CLA), known to possess various beneficial effects such as anticarcinogenic, antioxidative, and cholesterol-depressing, has been used as a health supplementary food in Japan and the USA. Optimum condition for CLA production without causing changes in quality of kimchi was determined using Bifidobacterium sp., a CLA-producing microorganism, as a starter in culture broth, freeze-dried culture, and encapsulated culture. Results revealed encapsulation was most ideal for maintaining the ability of bacterium to produce CLA during kimchi fermentation. Exogenous linoleic acid (LA) which is a substrate for conversion to CLA was not added to kimchi since LA was already exists in red pepper. Changes in sensory properties of kimchi and production of CLA were measured after inoculation of the encapsulated starter. The optimum inoculation concentration of the encapsulated starter was 0.1% (w/w) for production of CLA without causing changes in kimchi taste.

• Korean Journal Plant Pathology
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• v.1 no.3
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• pp.178-183
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• 1985

The present researches were carried out to investigate the peroxidase activity in association with the reactions of the 4 cultivars of rice plant, Nagdong, Jinheung, Nongbaek and Taebaek to Pyricularia oryzae race KJ-I0l and KJ-301. Although the peroxidase activity was increased during the growth of the rice seedlings, the significant difference in the activity was not found among 4 cultivars. After inoculation of the fungus, the peroxidase activity was enhanced in diseased leaves, being considerably higher in the compatible than in the incompatible cultivars. The isozyme bands of peroxidases observed in mycelium of rice blast fungus were not found in the diseased leaves on the gel electrophoresis. The peroxidase activity was not affected by the increased application of nitrogenous fertilizer.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.311-313
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• 2004

모짜렐라 치즈 제조 후 부산물로 생산된 유청에 가당을 하고 케휘어 그레인, 포도주 효모를 접종하여 새로운 발효유주 제조공정을 개발하고자 하였다. 그 결과 가당원료로서 물엿을 첨가한 유청 용액의 적정산도가 가장 높게 나타났으며, 첨가한 당의 종류별당 이용율은 배양초기에는 비교적 차이가 큰 편이었지만 배양시간이 경과할수록 차이가 감소하였다. 가당 유청 용액중에서 복합균주의 생육특성은 환원탈지유와는 달리 젖산구균의 생육이 우세하였다. 치즈 유청을 살균 후 당을 첨가하고 케휘어 배양액과 포도주 효모배양액을 접종하여 $20^{\circ}C$의 저온에서 알코올 발효를 실시하였을 때, 배양 1일부터 발포현상이 나타났으며 4일간 배양 후 1.2% 알코올을 함유하는 발효배양액을 얻을 수 있었다. 발효액을 2회에 걸쳐 증류농축기에서 $75^{\circ}C$에서 25분간 감압 하에서 알코올을 추출하여 20% 알코올 원액을 얻을 수 있었다.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.159.1-159
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• 2003

표준화된 청국장 제조법으로 대두를 정선, 수세 후 3배의 물을 가하여 24시간 침지하고 물빼기를 한 후 121$^{\circ}C$에서 1시간 동안 증자한 후 4$0^{\circ}C$까지 냉각한 후 재래청국장으로부터 분리한 B. lichenifomis 균주를 2% 접종, 4$0^{\circ}C$에서 54시간 발효시켜 청국장을 제조하였으며 발효 방법에 따라서는 종균을 접종한 경우가 가장 우수한 발효양상을 보였다. 감초추출물을 첨가하여 제조한 청국장의 품질특성을 알아본 결과 첨가농도가 증가할수록 pH가 증가하였으며 환원당 함량도 증가하였다. 색도는 첨가농도가 증가함에 따라 다소 어두워 졌으나 점질물의 생성량은 증가하는 경향이었다. 관능검사결과를 종합해 보면 5%의 첨가구에서 가장 높은 기호도를 보여 증자 대두에 감초추출물을 혼합하여 발효했을 경우 점질물의 생성의 증대는 물론 기호도의 향상에도 크게 기여하였다.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.2
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• pp.152-159
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• 1990

Four active strains, producing siderophore and antagonizing to soil plant disease fungi, were isolated and identified from the wheat rhizosphere to reduce the injurious effects of continuous cropping and study on the biological control. The obtained results were summerized as follow. 1. Four strains of fluorescent Pseudomonas were isolated from the wheat rhizosphere and identified as Pseudomonas fluorescens Biotype A(Ps-1,2,5) and Biotype B(Ps-3) That strains inhibitied growth of R. solani, F.oxysperum and F. solani in vitro 2. Optical density of pigment was maximum at 410nm. 3. Siderophore production by identified strains was decreased with addition of $Fe^{+3}$, although not decreased with addition of $Fe^{+2}$ 4. Pigment of Ps-1, 2 and 3 strain inhibitied growth of R. slani, F. oxysperum and F. solani but pigment of Ps-5 strain did not inhibitie growth of R. solani 5. Effect of inoculation was in order of Ps-2, 1, 5 and 3 strain through the dark culture method, and effect of Ps-1 and Ps-2 was greater than that of treatment of captan 50ppm.