• 건강소식
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• v.30 no.7 s.332
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• pp.22-23
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• 2006

장염의 원인은 매우 다양한데, 식중독과 같은 각종 바이러스성 감염과 세균 감염 등으로 인한 감염성 장염이 흔하며, 크론씨병, 궤양성 대장염과 같은 만성 질환도 있다. 주로 나타나는 증상은 복통과 설사이며 열이 동반되는 경우도 있다. 복통은 정확히 위치를 알 수 없는 묵직한 통증이 가장 흔하며, 묵직한 통증으로 시작하여 뒤틀리는 듯이 심하게 아픈 통증으로 진행하는 경우가 흔하다. 설사는 적게는 하루에 2~3회에서, 많게는 20여 회가 넘는 경우까지 그 빈도가 다양하다. 대장이 감염된 경우 변에 코와 같은 점액이 많이 섞여 나오고, 소장이 감염된 경우에는 점액이 별로 섞여 나오지 않는다. 주로 6개월~24개월 사이의 아이에게 잘 발생하며, 춥고 건조한 늦가을부터 겨울철(10월~1월)에 가장 많이 발생하는데, 우리나라에서는 시기에 관계없이 발생하기도 한다.

• Microbiology and Biotechnology Letters
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• v.32 no.1
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• pp.47-51
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• 2004

Drug resistance is one of the most significant impediments to successful chemotherapy of cancer. Multidrug-resistance Is characterized by decreased cellular sensitivity to anticancer agents due to the overexpression of P-glycoprotein. By using adriamycin-resistance CL02 cancer cells, we undertook the screening fur agents which were effective to multidrug-resistant cancer cells from strains of the species Sorangium cellulosum isolated in our laboratory. Sorangium cellulose, cellulose-degrading myxobacteria have recently proved to be a rich source of novel anticancer agents. One of the significant examples is the promising anticancer agent epothilone. JW 1006 is the first strain of Sorangium cellulosum which was selected by us for the isolation of a metabolite by a biological screening because of a high cytotoxic activity against the CL02 cancer cells. Cytotoxicity-guided chromatographic fractionation of the culture broth led to the Isolation of two active principles, disorazole $A_1$ and $A_2$. They showed potent cytotoxicity against CL02 cancer cells with $IC_{50}$ values in the picomolar range, and were as active against drug-resistant cancer cells CL02 and CP70 as against the corresponding sensitive cells.

• Microbiology and Biotechnology Letters
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• v.41 no.1
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• pp.44-51
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• 2013

We prepared culture extracts of 174 Corallococcus and 207 Myxococcus strains isolated in Korea, and compared their antimicrobial activity against Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus. The percentage of strains showing antifungal activity was lower in Corallococcus (7.5% [13 of the 174 strains]) than in Myxococcus (51.7% [107 of the 207 strains]). However, the percentage of strains exhibiting antibacterial activity was higher in Corallococcus (12.1% [21 strains]) than in Myxococcus (1% [2 strains]). The culture extracts of 6 Corallococcus strains inhibited both P. aeruginosa and S. aureus and displayed similar high-performance liquid chromatography chromatograms, although the shapes of their fruiting bodies were dissimilar. The rate of production of antibacterial substances was the highest when the strains were cultured in CYS medium for more than 6 days.

• 건강소식
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• v.36 no.1
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• pp.24-25
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• 2012

여러 가지 실버 세대 질환 중 폐렴은 발병률이 높고 발병시 사망률도 높은 것으로 알려졌다. 실버 세대에서 폐렴의 위험이 높은 이유는 면역기능의 저하, 인후부반사기능의 저하, 점액섬모운동의 기능 저하 및 여러 가지 심폐기능부전 등에 관여하기 때문이다. 노인성폐렴에 대해 알아보자.

• Microbiology and Biotechnology Letters
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• v.39 no.1
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• pp.20-28
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• 2011

Phylogenetic analysis of two cellulase genes, xynB1 and bglA2, and the groEL1 gene from 34 Sorangium cellulosum strains isolated in Korea suggested that there are at least five subgroups in S. cellulosum, which is the most proficient producer of secondary metabolites among myxobacteria. This analysis also revealed diversity among the isolated S. cellulosum. It appeared that at least 30 out of 34 strains are different each other.

• Korean Journal Plant Pathology
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• v.14 no.4
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• pp.345-349
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• 1998

Fifty isolates of myxobacteria were isolated from soils from several areas in Korea during 1996-1997 and bioactivity against plant pathogenic fungi of these isolates was examined. A myxobacterial isolate KR025 showed good antifungal activities against Pyricularia oryzae, Cryphonectria parasitica, Colletotrichum lagenarium, and C. gloeosporioides but did not against Rhizoctonia solani, Fusarium oxysporum and Pythium ultimum. The bacterium was identified as Myxococcus fulvus based on morphological and physiological characteristics. Antifungal substances were extracted from culture broth and bacterial cell of Myxococcus fulvus KR025 by ethyl acetate. Antifungal substance of Myxothiazole (100 ${\mu}{\textrm}{m}$/ml) produced by Myxococcus fulvus KR 025 controlled 97.0% rice blast, tomato late blight, wheat leaf rust, and barley powdery mildew and showed 45.0 and 82.6% disease control of rice sheath blight and cucumber gray model, respectively.

• Microbiology and Biotechnology Letters
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• v.34 no.4
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• pp.335-337
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• 2006

Glycerol-spore formation of wild myxobacteria isolated in Korea has been tested. One third of the wild myxobacterial isolates belonged to the genus Myxococcus and Corallococcus produced a large number of glycerol-spores in the presence of glycerol or dimethyl sulfoxide. However, two third of the other wild myxobacterial isolates belonged to the genus Myxococcus and Corallococcus produced a negligible number of spores under the same condition. In addition, the myxobacteral isolates belonged to the other genus, such as Stigmatella, Archangium, and Cystobacter, failed to produce a noticeable number of glycerol-spores.

• Journal of Conservation Science
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• v.21
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• pp.59-72
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• 2007

The relationship between soluble salts and microbial growth on stone surface was investigated. Samples from deteriorated surface layers of several stone monuments were collected, and the soluble salts in samples were chemically analysed. According to the results of chemical analyses, $Na_2SO_4$, $CaSO_4$ $2H_2O$, and NaCl and so on were added to isolation media. As a result of preliminary experiments, 15 bacteria were selected. They are all Gram positives and fourteen of them were rods. Every bacteria could grow not only in media without salts, but also in media with $CaCO_3$, $CaSO_4$ $2H_2O$, and $Ca(OH)_2$. Three bacteria, Bl6, B56, and B6l, secreted viscous substances in these $Ca^{2+}$ added media. $CaCl_2$ $2H_2O$ was the most hygroscopic among 11 soluble salts, but it seemed to interfere with bacterial growth. Ammonium ion was the inhibitor, too.

• Journal of fish pathology
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• v.35 no.1
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• pp.57-63
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• 2022

Vibrio parahaemolyticus and V. vulnificus are known to be infected to human via fisheries products. Therefore, food safety of fisheries products is important for public health and fish industry. This paper was conducted to know how well these human isolates can survive in olive flounder (Paralichthys olivaceus). The growth of V. parahaemolyticus and V. vulnificus showed about 50~60% reduced rates at 25℃ than at 37℃ and did not show any differences according to NaCl concentration of media except the increasing in the growth of V. vulnificus in medium containing 3% NaCl. Artificial infection of 1×10⁶ CFU/fish was carried out to confirm the sensitivity of olive flounder against V. parahaemolyticus and V. vulnificus. After 1 week from injection, no fish was dead. To evaluate nonspecific defense of olive flounder against V. parahaemolyticus and V. vulnificus, the antibacterial potency of serum and epidermal mucus were tested. The number of the vibrios exposed to serum obtained from olive flounder significantly decreased after 3 hours, and epidermal mucus showed decrease of the bacteria over than 90% until 12 hours from exposure. Phagocytosis of head kidney leucocytes of healthy olive flounder against V. parahaemolyticus and V. vulnificus showed in over 70% of leucocytes at the 2 hours. Therefore, cultured olive flounder only as vehicle for human pathogen in environmental water is well developed its antibacterial potency against human pathogens, so the viability of V. parahaemolyticus and V. vulnificus in cultured olive flounder was considered very low.

• Applied Biological Chemistry
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• v.40 no.3
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• pp.173-177
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• 1997

Bacillus licheniformis 9945a releases a natural ${\gamma}-poly(glutamic\;acid)({\gamma}-PGA)$ into fermentation broth and shows a mucoid phenotype on the solid agar medium. Transformation of mucoid cells of Bacillus species has not been simple and straightforward. The transpositional activity of Tn10 in B. licheniformis also has not been own either. Thus, a spontaneous non-mucoid derivative of the B. licheniformis was obtained first. Shuttle vector pHV1248 containing mini-Tn10 was introduced into the non-mucoid derivative by the method of protoplast transformation. The resulting transformant was reverted to the wild mucoid phenotype, and then mutated randomly with the mini-transposon by heat induction. Auxotrophs requiring arginine, lysine, or tryptophan were isolated by replica plating method. Southern blotting and DNA-DNA hybridzation analysis showed that these auxotrophs were generated by mini-Tn10 insertion into the chromosomal DNA. This method of transformation and mutation using pHV1248 would be useful for the generation of diverse mutants of B. licheniformis 9945a.(Received January 24,1997; accepted March 10, 1997)