• Proceedings of the Korean Society of Tobacco Science Conference
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• 2001.05a
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• pp.72-78
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• 2001

농업 유전공학 기술은 생산성 향상, 환경보전, 식품의 안정성 및 품질향상에 기여함은 물론 농업의 경쟁력을 높일 수 있는 유일한 대안으로 인식되고 있다. 전 세계적으로 유전자 재조합 작물의 경작지는 2000년 한해동안 지난해 같은 기간에 비해 11% 증가하였으며 이는 1996년 대비 25배 증가하였고, 선진국과 개발도상국은 각각 2%, 51% 1999년 대비 증가하였다. 1983년 유전자 재조합에 의한 식물의 형질전환이 성공한 뒤 종묘업계는 형질전환 종자개발과 보급에 열중하고 있으며 종자시장에 형질전화 품종이 차지하는 비율은 2000년 30억 달러에서 2010년이면 전체의 60%인 200억 달러에 이를 것으로 전망된다. 1995년 제초제 저항성 콩(라운드업레디콩)이 농가에 보급된 이후 2000년 형질전환품종 재배면적이 3990만 ha에 이르렀고 1997년 미국과 캐나다는 옥수수, 대두, 면화, 감자, 유채 등의 형질전환 품종 재배로 각각 3억1400만 달러, 5300만 달러를 벌어들였음. 형질전환 품종의 보급 증가속도는 소비자들의 GMO에 대한 거부반응으로 다소 주춤한 상태이다. 그러나 최근 종자회사들은 생태계 위해성 논란을 피해갈 수 있는 연구로 이러한 상황을 돌파하려 하고 있다. 우리나라에서도 유전자변형 생물체에 관한 법률이 제정되었으며 많은 대학과 연구소에서 형질전환 연구가 꾸준히 이루어지고 있고 최근 제초제 저항성 벼와 바이러스 저항성 감자가 개발돼 GMO 안정성 점검에 들어가 있고, 살충성 배추, 혈압강하 토마토, 지방산 강화 들깨, 병저항성 고추 등도 실험실과 포장에서 재배되고 있다. 이르면 4-5년 뒤 형질전환 작물들이 농가에 보급될 전망이다. 이처럼 체크 툴은 Firewall의 수비능력을 보강하는 위치에 있다고 생각할 수 있다.다. 4 장에서는 3장에서 제기한 각각의 문제점에 대해 RAD 의 관점에 비추어 e-business 시스템의 단기개발을 실현하기 위한 고려사항이나 조건 해결책을 제안한다. 본 논문이 지금부터 e-business 를 시작하려고 하는 분, e-business 시스템의 개발을 시작하려고 하는 분께 단기간의 e-business 실현을 위한 하나의 지침이 된다면 다행이겠다.formable template is used to optimize the matching. Then, clustering the similar shapes by the distance between each centroid, papaya can be completely detected from the background.uage ("Association of research for algorithm of calculating machine (1992)"). As a result, conventional NN and CNN were available for interpolation of sampling data. Moreover, when nonlinear intensity is not so large under the field condition of small slope, interpolation performance of CNN was a little not so better than NN. However, when nonlinear intensity is large under the field condition of

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.408-413
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• 2010

The paper reviewed research status and outlook of genetic transformation in fruit trees in Korea. Genetic transformation has been long considered as an alternative technique overcoming limitation of conventional breeding and conducted since early 1990's. An efficient genetic transformation was established with major cultivars of apple, even in Fuji and Gamhong, and some transgenic apple plants have been transferred into the greenhouse for further analysis of gene expression. A few successes of genetic transformation have been reported and application to a variety of cultivars tried in citrus and kiwifruit. Successful genetic transformation has not been reported in the other fruit trees including grapevine, yet and it is considered being at the level of research. Those factors including replacement of antibiotics as a selective agent, use of transgenic rootstocks and manipulation of gene expression at proper parts and developmental stages have been prerequisites for the rapid commercialization of transgenic fruit plants.

• Journal of Digital Convergence
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• v.20 no.2
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• pp.91-105
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• 2022

Most of the preceding studies related to Internet Primary banks are studies on laws, regulations, and expected effects of introduction, and studies on financial consumers' intention to switch to financial services are insufficient. Apply to the PPM(Push-Pull-Mooring)theory to find out the factors that influence financial consumers' intention to switch services from commercial banks to Internet Primary banks. A survey was conducted service users, 1st-order and 2nd-order factor analysis were performed using Smart PLS 3.0. As a result, it was confirmed that the Pull, Push and Mooring had a positive (+) effect on the Intention to Switch, and the Mooring, which is a moderating variable, did not have a moderating effect on the Intention to Switch of the Push and the Pull. The scope of application of the PPM theory, which was used in the service conversion research, was extended to Fintech services, and it can provide various practical useful implications, such as the strategy and spread of Internet Primary banks, and it will be used in various studies to study consumer attitudes.

• Applied Biological Chemistry
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• v.51 no.3
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• pp.177-182
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• 2008

This study was carried out to investigate the reaction of lipase-catalyst transesterification using animal fat recovered from fleshing scrap generated during leather making process. Transesterification reaction between fat and primary or secondary alcohol was carried out under the condition of immobilized enzyme catalyst. The conversion rate was the highest when 1.5 mole of methanol was injected by 4 times. As for lipase, Candida antarctica showed the highest conversion rate of 82.2% among the 4 different lipases. It was found that water contained in the fat causes lower conversion rate. The condition of 1.2wt. % of water in the fat decreased the conversion rate by 40%. It was considered that the resulted reactant, fatty acid ester could be used as raw material for biodiesel with the characteristics of not generating SOx and diminishing smoke.

• Proceedings of the KIPE Conference
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• 2016.07a
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• pp.113-114
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• 2016

영구자석형 동기전동기를 구동하는 인버터에서는 기본적으로 정현파 운전 방식을 사용하지만 최근에는 구형파 운전 방식도 종종 사용되고 있다. 정현파 운전 방식은 구형파 운전 방식에 비해 토크 리플이 작고 기동 성능이 우수하며, 약자속 제어를 이용하여 정격 속도 이상의 고속 운전이 가능하다. 이에 비해 구형파 운전 방식은 제어 알고리즘이 간단하고 스위칭 손실이 적은 장점이 있지만, 토크 리플이 크고 정격 속도 이하의 영역에서만 운전이 가능하다는 단점이 있다. 최근에 인버터의 운전 중에 각각의 장점이 있는 이들 2가지 운전 방식을 서로 전환하는 방법으로 가전제품의 압축기와 같은 연속 부하에 적용하기 위한 연구가 진행되었다. 저속 구간에서는 스위칭 손실을 줄이기 위하여 구형파 운전 방식을 주로 사용하고, 고속 구간에서는 안정적인 제어 성능을 위하여 정현파 운전 방식을 사용한다. 또한, 이 2가지 운전 방식의 전환 구간에서의 발생되는 과도 상태를 짧게 줄이기 위하여 속도 및 전류 제어기의 제어 변수에 대한 초기화 방법이 제시되었다. 본 논문에서는 구형파 운전 구간의 속도 제어기에 비례 적분 전류 제어기를 추가하여 전류 리플을 감소시키고, 전환 구간에서의 속도 및 전류 제어기의 제어 변수에 대한 초기화 방법을 제시하였다.

• Journal of the Korea Society for Simulation
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• v.22 no.2
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• pp.85-91
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• 2013

Smart phones become essential goods for our life. As the technology are developing, and the market becomes saturated, it is important to understand the determinants affecting purchase of smart phones. In this paper, to understand these determinants factor analysis and binary logistics regression is used. Toward this, we first define the determinants into 3 categories: customer satisfaction, switching barrier, and switching attractiveness based on literature review. Furthermore, we develop 9 hypotheses to test with survey data using factor analysis and binary logistics regression. The results show that the six factors (data quality, price satisfaction, corporate image, the burden of new contract, attraction of devices and attraction of other competitors) are significant when customers buy smart phones.

• Journal of the Korean Data and Information Science Society
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• v.28 no.4
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• pp.843-851
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• 2017

The rise of the Robo-advisor represents one of the most profound shifts in FinTech. It also raises concerns about their financial management. As the most Robo-Advisors utilize ETFs, we seek to determine the appropriate risk management model in estimating 95% Value-at-Risk (VaR) and 99% VaR in this paper. The GARCH and the Markov regime wwitching GARCH are evaluated in terms of the accuracy of probability, the independence of extreme events occurrence and both. The result shows that the Markov regime switching GARCH can be a good ETF risk management tool since it can reflect financial market structural changes into the volatility.

• Proceedings of the Korea Information Processing Society Conference
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• 2020.11a
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• pp.1090-1093
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• 2020

운전자가 아닌 자율주행 시스템이 운전을 주도하기 위한 기술의 상용화를 위해 많은 기업이 노력 중이다. 특히 운전자의 안전을 보장하기 위한 운전자와 자율주행 시스템 간의 제어권전환이 중요하다. 운전자의 주행과 관련 없는 행동은 제어권전환 상황에서 운전자를 위험에 빠뜨릴 수 있으므로 제어권전환을 돕기 위한 운전자 모니터링 기술에 관한 많은 연구가 진행되고 있다. 운전자 모니터링 기술은 주로 생체 정보, 차량 정보, 영상을 사용하여 운전자의 상태와 부주의 행동 등을 감지하는 기술이다. 최근 머신 러닝, 딥 러닝을 사용한 영상처리 및 인식 기술 등의 발전으로 영상을 사용한 운전자 모니터링 기술이 활발하게 연구되고 있다. 따라서 본 논문에서는 영상기반 운전자 모니터링 기술 동향에 대해 상세히 기술하였다. 특히 운전자의 부주의 행동 중 졸음은 운전자가 주행 상황을 전혀 인지하지 못하게 할 수 있어 더욱 위험한 행동이다. 따라서 영상기반 운전자 모니터링 기술을 졸음 인식과 그 외의 행동 인식으로 분류하여 동향을 정리하였다.

• Korean Journal of Environmental Biology
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• v.18 no.2
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• pp.205-213
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• 2000

Laboratory studies have revealed that naturally transformable bacteria develop competence under in situ conditions. Thus, the occurrence of competent bacteria in the environment can be considered as a certainty The persistence of free DNA in natural habitats is influenced by nucleolytic degradation and protection from degradation by adsorption to minerals. Although DNA seeded into natural environment was hydrolysed at substantial rates, but was still detectable at low levels after even several weeks. Compared to the number of laboratory based studies, only a few data have been published dealing with transformation of bacteria in the field. Recently, the potential transfer of recombinant DNA (rDNA) from deliberately or accidentally released bacteria to indigenous microbes has raised biosafety issues, since the persistence of rDNA becomes independent of the survival of its original host and leads to unpredictable, long-term ecological effects. The aim of the present review is to summarise recent literature on horizontal gene transfer (HGT) by transformation among bacteria in both soil and aquatic habitat and special emphasis is placed on recent reports which have addressed HGT among bacteria in the field. [Transformation, Horizontal gene transfer (HGT), recombinant DNA (rDNA), Genetically modified microorganisms (GMMs), Biosafety]

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.37-45
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• 2007

The genetic transformation of watermelon by Agrobacterium has been known very difficult and a few successful cases have been reported by obtaining the direct shoot formation. However, since this direct shoot formation is not guaranteed the stable transformation, the stable transformation with reproducibility is required by a different approach such as a callus induced manner. The best conditions for inducing the callus from cotyledon and root explants of watermelon were 2 mg/L zeatin + 0.1 mg/L IAA and 2 mg/L BA + 0.1 mg/L 2,4-D, respectively. The GFP expression in the callus was identified and monitored through fluorescent microscopy after transformation with pmGFP5-ER vector. Paromomycin rather than kanamycin was used for selecting the nptll gene expression because it was more effective to select the watermelon explants. Four different callus types were observed and the solid green callus showed stronger GFP expression. The highest frequency of GFP expression in the callus developed from cotyledon was 9.0% (WM8 inbred line), while the highest frequency from root was 8.3% (WM6 inbred line). The WMV-CP was transformed using the method of GFP transformation and the genetic transformation of WMV-CP was confirmed by PCR and Southern blot analysis. Here we present a system for callus induction of watermelon explant and the callus induced method would facilitate the establishment of stable watermelon transformation.