• Title/Summary/Keyword: 전체 유전체

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PCR-based markers for discriminating Solanum demissum were developed by comparison of complete chloroplast genome sequences of Solanum species (가지속 식물의 엽록체 전장유전체 비교를 통한 PCR 기반의 Solanum demissum 특이적 분자마커 개발)

  • Park, Tae-Ho
    • Journal of Plant Biotechnology
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    • v.48 no.1
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    • pp.18-25
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    • 2021
  • Solanum demissum is one of the wild Solanum species originating from Mexico. It has wildly been used for potato breeding due to its resistance to Phytophthora infestans. S. demissum has an EBN value of four, which is same as that of S. tuberosum, so that it is directly crossable for breeding purposes with the cultivated tetraploid potato (S. tuberosum). In this study, the chloroplast genome sequence of S. demissum obtained by next-generation sequencing technology was described and compared with those of seven other Solanum species to develop S. demissum-specific markers. Thetotal sequence length of the chloroplast genome is 155,558 bp, and its structural organization is similar to those of other Solanum species. Phylogenetic analysis with ten other Solanaceae species revealed that S. demissum is most closely grouped with S. hougasii and S. stoloniferum followed by S. berthaultii and S. tuberosum. Additional comparison of the chloroplast genome sequence with those of seven other Solanum species revealed two InDels specific to S. demissum. Based on these InDels, two PCR-based markers for discriminating S. demissum from other Solanum species were developed. The results obtained in this study will provide an opportunity to investigate more detailed evolutionary and breeding aspects in Solanum species.

Current Status and Prospect of Wheat Functional Genomics using Next Generation Sequencing (차세대 염기서열분석을 통한 밀 기능유전체 연구의 현황과 전망)

  • Choi, Changhyun;Yoon, Young-Mi;Son, Jae-Han;Cho, Seong-Woo;Kang, Chon-Sik
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.364-377
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    • 2018
  • Hexaploid wheat (common wheat/bread wheat) is one of the most important cereal crops in the world and a model for research of an allopolyploid plant with a large, highly repetitive genome. In the heritability of agronomic traits, variation in gene presence/absence plays an important role. However, there have been relatively few studies on the variation in gene presence/absence in crop species, including common wheat. Recently, a reference genome sequence of common wheat has been fully annotated and published. In addition, advanced next-generation sequencing (NGS) technology provides high quality genome sequences with continually decreasing NGS prices, thereby dawning full-scale wheat functional genomic studies in other crops as well as common wheat, in spite of their large and complex genomes. In this review, we provide information about the available tools and methodologies for wheat functional genomics research supported by NGS technology. The use of the NGS and functional genomics technology is expected to be a powerful strategy to select elite lines for a number of germplasms.

The Brassica rapa Tissue-specific EST Database (배추의 조직 특이적 발현유전자 데이터베이스)

  • Yu, Hee-Ju;Park, Sin-Gi;Oh, Mi-Jin;Hwang, Hyun-Ju;Kim, Nam-Shin;Chung, Hee;Sohn, Seong-Han;Park, Beom-Seok;Mun, Jeong-Hwan
    • Horticultural Science & Technology
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    • v.29 no.6
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    • pp.633-640
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    • 2011
  • Brassica rapa is an A genome model species for Brassica crop genetics, genomics, and breeding. With the completion of sequencing the B. rapa genome, functional analysis of the genome is forthcoming issue. The expressed sequence tags are fundamental resources supporting annotation and functional analysis of the genome including identification of tissue-specific genes and promoters. As of July 2011, 147,217 ESTs from 39 cDNA libraries of B. rapa are reported in the public database. However, little information can be retrieved from the sequences due to lack of organized databases. To leverage the sequence information and to maximize the use of publicly-available EST collections, the Brassica rapa tissue-specific EST database (BrTED) is developed. BrTED includes sequence information of 23,962 unigenes assembled by StackPack program. The unigene set is used as a query unit for various analyses such as BLAST against TAIR gene model, functional annotation using MIPS and UniProt, gene ontology analysis, and prediction of tissue-specific unigene sets based on statistics test. The database is composed of two main units, EST sequence processing and information retrieving unit and tissue-specific expression profile analysis unit. Information and data in both units are tightly inter-connected to each other using a web based browsing system. RT-PCR evaluation of 29 selected unigene sets successfully amplified amplicons from the target tissues of B. rapa. BrTED provided here allows the user to identify and analyze the expression of genes of interest and aid efforts to interpret the B. rapa genome through functional genomics. In addition, it can be used as a public resource in providing reference information to study the genus Brassica and other closely related crop crucifer plants.

Effective Analysis Of SNP Related Chronic Hepatitis Using SNP (SVM을 이용한 만성간염 환자 예측진단을 위한 SNP 정보분석)

  • Kim Dong-Hoi;Ham Ki-Baek;Kim Jin
    • Proceedings of the Korean Information Science Society Conference
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    • 2006.06a
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    • pp.19-21
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    • 2006
  • Single Nucleotide Polymorphism(SNP)는 인간 유전자 서열의 0.1%에 해당하는 부분으로 이는 각 개인의 체질 및 각종 유전질환과 밀접한 관련이 있다고 알려져 있다. 최근 이 SNP정보의 패턴을 이용 질병의 진단 및 치료에 연관지으려는 노력이 시도되고 있다. 그러나 아직 SNP를 이용한 효율적인 분석방법에 대한 전산학적 연구는 많지 않다. 본 논문에서는 대표적인 패턴인식기 중 하나인 Support Vector Machine(SVM)을 이용 한국인의 대표적인 유전질환으로 알려진 만성간염에 대해서 관련된 SNP에 대한 패턴 인식율 측정을 실험하였다. 실험 데이터는 간 및 소화기 질환 유전체 센터에서 얻어진 만성간염 환자와 관련 SNP정보를 사용하였으며, 실험 결과 전체 SNP 정보를 모두 가지는 환자그룹에 대한 학습인식율이 66.46%로 나타났으며, 부분그룹에서는 72.91%로 높은 인식율을 보였다. 이 결과는 SNP 정보를 이용한 만성간염의 초기진단예측에 SVM을 효율적으로 사용할 수 있음을 보인다.

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Genetic Diversity and Genetic Structure of Phellodendron amurense Populations in South Korea (황벽나무 자연집단의 유전다양성 및 유전구조 분석)

  • Lee, Jei-Wan;Hong, Kyung-Nak;Kang, Jin-Taek
    • Journal of Korean Society of Forest Science
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    • v.103 no.1
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    • pp.51-58
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    • 2014
  • Genetic diversity and genetic structures were estimated in seven natural populations of Phellodendron amurense Rupr in South Korea using ISSR markers. The average of polymorphic loci per primer and the proportion of polymorphic loci per population were 4.5 and 78.8% respectively with total 27 polymorphic loci from 6 ISSR primers. The Shannon's diversity index(I) was 0.421 and the expected heterozygosity($H_e$) was 0.285, which was similar to the heterozygosity (hs =0.287) inferred by Bayesian method. In AMOVA, 7.6% of total genetic variation in the populations was resulted from the genetic difference among populations and the other 92.4% was resulted from the difference among individuals within populations. Genetic differentiation(${\theta}^{II}$) and inbreeding coefficient(f) for total population were estimated to be 0.066 and 0.479 by Bayesian method respectively. In Bayesian clustering analysis, seven populations were assigned into three groups. This result was similar to the results of genetic relationships by UPGMA and PCA. The first group included Hwachoen, Gapyeong, Bongpyeong and Yongpyeong population, and the second included two populations in Sancheong region. Muju population was discretely assigned into the third group in spite of the geographically short distance from the Sancheong region. There was no significant correlation between genetic relationship and geographic distribution among populations in Mantel's test. For conservation of the phellodendron trees, it would be effective to consider the findings resulted from this study with ecological traits and life histories of this species.

Application of Transposable Elements as Molecular-marker for Cancer Diagnosis (암 진단 분자 마커로서 이동성 유전인자의 응용)

  • Kim, Hyemin;Gim, Jeong-An;Woo, Hyojeong;Hong, Jeonghyeon;Kim, Jinyeop;Kim, Heui-Soo
    • Journal of Life Science
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    • v.27 no.10
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    • pp.1215-1224
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    • 2017
  • Until now, various oncogenic pathways were idenfied. The accumulation of DNA mutation induces genomic instability in the cell, and it makes cancer. The development of bioinformatics and genomics, to find the precise and reliable biomarker is available. This biomarker could be applied the early-dignosis, prediction and convalescence of cancer. Recently, Transposable elements (TEs) have been attracted as the regulator of genes, because they occupy a half of human genome, and the cause of various diseases. TEs induce DNA mutation, as well as the regulation of gene expression, that makes to cancer development. So, we confirmed the relationship between TEs and colon cancer, and provided the clue for colon cancer biomarker. First, we confirmed long interspersed nuclear element-1 (LINE-1), Alu, and long terminal repeats (LTRs) and their relationship to colon cancer. Because these elements have large composition and enormous effect to the human genome. Interestingly, colon cancer specific patterns were detected, such as the hypomethylation of LINE-1, LINE-1 insertion in the APC gene, hypo- or hypermethylation of Alu, and isoform derived from LTR insertion. Moreover, hypomethylation of LINE-1 in proto-oncogene is used as the biomarker of colon cancer metastasis, and MLH1 mutation induced by Alu is detected in familial or hereditary colon cancer. The genes, effected by TEs, were analyzed their expression patterns by in silico analysis. Then, we provided tissue- and gender-specific expression patterns. This information can provide reliable cancer biomarker, and apply to prediction and diagnosis of colon cancer.

Development of Solanum hougasii-specific markers using the complete chloroplast genome sequences of Solanum species (엽록체 전장유전체 정보를 이용한 Solanum hougasii 특이적 분자마커 개발)

  • Kim, Soojung;Park, Tae-Ho
    • Journal of Plant Biotechnology
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    • v.47 no.2
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    • pp.141-149
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    • 2020
  • Solanum hougasii, one of the wild Solanum species, has been widely used in potato breeding since it exhibits excellent resistance to diverse important pathogens. S. hougasii can be directly crossed with the cultivated tetraploid potato (S. tuberosum) owing to its EBN (Endosperm Balanced Number) value of 4, which is same as that of S. tuberosum although it is an allohexaploid. In this study, the complete chloroplast genome sequence of S. hougasii was obtained by next-generation sequencing technology, and compared with that of the chloroplast genome of seven other Solanum species to identify S. hougasii-specific PCR markers. The length of the complete chloroplast genome of S. hougasii was 155,549 bp. The structural organization of the chloroplast genome in S. hougasii was found to be similar to that of seven other Solanum species studied. Phylogenetic analysis of S. hougasii with ten other Solanaceae family members revealed that S. hougasii was most closely related to S. stoloniferum, followed by S. berthaultii, and S. tuberosum. Additional comparison of the chloroplast genome sequence with that of five other Solanum species revealed five InDels and 43 SNPs specific to S. hougasii. Based on these SNPs, four PCR-based markers were developed for the differentiation of S. hougasii from other Solanum species. The results obtained in this study will aid in exploring the evolutionary and breeding aspects of Solanum species.

An inspection of stability for annealing SiOCH thin flim (SiOCH 박막의 열처리에 대한 안정성 검토)

  • Park, Yong-Heon;Kim, Min-Seok;Hwang, Chang-Su;Kim, Hong-Bae
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.06a
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    • pp.41-42
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    • 2008
  • p-type(100) Si 위에 BTMSM과 산소를 혼합한 전구체를 가지고 PECVD 방법을 사용 하여 저유전상수를 갖는 SiOCH막을 형성하였다. 알루미늄 전극을 구현한 MIS (Al/SiOCH/p-si(100)) 구조의 커패시터를 가지고 C-V 특성을 측정하여 유전상수를 계산하였다. 상온에서 증착된 SiOCH 박막의 유전상수는 $450^{\circ}C$에서 30분 동안 열처리 후 뚜렷하게 감소하는 경향을 나타냈으며, 상온 및 대기압에서 공기 중에 노출시켜 자연 산화과정을 겪은 후에 각각의 유전상수는 전체적으로 증가하였지만, 열처리한 박막이 상대적으로 경시효과(aging effects)에 대하여 안정화된 것을 확인하였다.

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Simultaneous Switching Noise Reduction Technique in Multi-Layer Boards using Conductive Dielectric Substrate (전도성 유전기판을 이용한 다층기판에서의 Simultaneous Switching Noise 감소 기법)

  • 김성진;전철규;이해영
    • Journal of the Microelectronics and Packaging Society
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    • v.6 no.4
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    • pp.9-14
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    • 1999
  • In this paper, we proposed a simultaneous switching noise (SSN) reduction technique in multi-layer boards (MLB) for high-speed digital applications and analyzed it using the Finite Difference Time Domain (FDTD) method. The new structure using conductive dielectric substrates is effective for the reduction of SSN couplings and resonances. The uniform insertion of the conducive layer reduced the SSN coupling and resonance by 85% and the partial insertion only around the edges reduced by 55% respectively.

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Efficient Sequence Association Rule Mining for Discovering Protein Relations (단백질 서열 연관 규칙 마이닝을 위한 효율적인 알고리즘 설계)

  • Kim, Hyun-Min;Kim, Ji-Hye;Ramakrishna, R.S.
    • Proceedings of the Korea Information Processing Society Conference
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    • 2002.04b
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    • pp.1183-1186
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    • 2002
  • DNA 의 염기서열 탐색을 위한 유전체학의 다음 세대인 구조유전체학은 유전체 사업으로 인한 인간 게놈지도의 완성과 축적된 생물정보를 이용한 생물정보학의 발달과 함께 급속한 성장을 계속하고 있다. 포스트 게놈 시대를 맞이하여 생명현상에 대한 궁극적인 이해를 위한 노력으로 단백질의 구조와 기능에 대한 연구가 주목을 받게 되었다. 다양한 구조 규명을 위한 도구들과 단백질 정보를 관리하기 위한 데이터베이스 구축에 따른 관련 기술의 발전은, 앞으로 다가올 생물정보의 방대함을 감안할 때, 가치 있는 지식정보를 얻기 위한 데이터 마이닝 기법들을 통해서만 가능하다. 본 논문은 데이터 마이닝의 근간 기술인 연관규칙 마이닝을 응용한 효율적인 서열 연관 규칙 알고리즘을 제안하며, 단백질 구조의 예측을 위한 단백질 서열 및 DNA 서열간의 패턴 비교 및 연관성을 목적으로 한다. 또한, 공간적 시간적 복잡성을 CMS-tree 라는 자료구조를 통해 알고리즘의 확장성 및 병렬화의 기본 알고리즘으로 사용하도록 개발하였다.

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