• Applied Biological Chemistry
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• v.38 no.5
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• pp.435-439
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• 1995

Some characteristics and pathogenicity of Hyphantria cunea nuclear polyhedrosis virus (HcNPV), a potential microbial pesticide was studied. H. cunea NPV replicated in the nucleus of S. frugiperda cells cultured in the TNMFH medium. In case of virus infected cell, prepolyhedra formation was observed at 24hrs post-infection. At 48 hrs post-infection, Most of the infected cell contained many mature polyhedra which were released into culture media 72 hrs post-infection, with the cells grown in suspension culture, pH of the culture medium increased during the virus replication: the pH of fresh medium was 6.35 and rose to 6.77 within 120 hrs. Polyhedra formed a band in linear density gradient of sucrose by centrifugation, which co-sedimented with $50{\sim}55%$ sucrose. The shape of the purified polyhedra was mostly tetragonal hexahedron and its size was about $2.5{\mu}m$. Electron microscopy and phase contrast microscopy showed that many bundled nucleocapsids were occluded in mature polyhedra at 48 hrs post infection. H. cunea larvae infected with NPV showed a higher motality in the second and third instar than in the fourth instar. Death rate of H. cunea larvae in the second and third instar fed with leaves coated with $1.5{\times}10^{9}{\sim}l.5{\times}10^{7}PIBs/ml$ reached more than 90%.

• Korean Journal of Agricultural and Forest Meteorology
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• v.6 no.3
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• pp.170-176
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• 2004

This study was carried out to establish a spatial decision support system for evaluating climatic aspects of a given geographic location in complex terrains with respect to the quality apple production. Monthly climate data from S6 synoptic stations across South Korea were collected for 1971-2000. A digital elevation model (DEM) with a 10-m cell spacing was used to spatially interpolate daily maximum and minimum temperatures based on relevant topoclimatological models applied to Jangsoo county in Korea. For daily minimum temperature, a spatial interpolation scheme accommodating the potential influences of cold air accumulation and the temperature inversion was used. For daily maximum temperature estimation, a spatial interpolation model loaded with the overheating index was used. Freezing risk in January was estimated under the recurrence intervals of 30 years. Frost risk at bud-burst and blossom was also estimated. Fruit quality was evaluated for soluble solids, anthocyanin content, Hunter L and A values, and LID ratio, which were expressed as empirical functions of temperature based on long-term field observations. AU themes were prepared as ArcGlS Grids with a 10-m cell spacing. Analysis showed that 11 percent of the whole land area of Jangsoo county might be suitable for quality 'Fuji' apple production. A computer program (MAPLE) was written to help utilize the results in decision-making for site-selection of new orchards in this region.

• Geophysics and Geophysical Exploration
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• v.12 no.4
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• pp.299-308
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• 2009

A magentotelluric (MT) survey at the Uiseong area has been performed for the site investigation of pilot scale $CO_2$ sequestration. The purpose of the MT survey is to delineate deeply extended fracture systems that can act as a leakage path of injected $CO_2$ Plume. Since the target area is extremely noisy in electromagentic sense, low frequency data below 1 Hz cannot be used for inversion. Two- and three-dimensional interpretation of the MT data showed a very clear conductive anomaly, which has the direction of $N55\sim65^{\circ}W$ and is extended roughly down to 1.6 km. It have the same direction with the strike-slip faults, the Gaeum and Geumcheon Faults. On the contrary, the eastern part of the survey area shows relatively homogeneous to the depth of 2 km though some small fractures at shallow depths can be found. Test drilling and high-definition borehole surveys should be followed at the eastern part of the survey area and hydraulic fracturing is required for injection of $CO_2$, because mean porosity of the sedimetary rock in the area is only 1.47%.

• Journal of the Korean Association of Geographic Information Studies
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• v.7 no.2
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• pp.57-68
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• 2004

Recently in order to more effectively and scientifically process forest official tasks, which have been focused on documents and inventories, they should be applied with the up-to-date spatial information technologies. Especially, the forest resource information management based on GIS(geographic information system) and aerial orthophoto is expected not only to play an important role as DSS(decision support system) for domestic forest conservation policy and forestry development industry but also to service forest resource information toward people such as the owners of a mountain rapidly. In this study, the important forest information such as digital topography map, digital forest type map, digital forest cadastral map, digital aerial photographs and attribute data were first reprocessed and constructed in DBMS(data base management system). In addition, forest officials could analyze and retrieve forest information by using detail sub-application systems such as forest cadastral retrieval, forest land development information management, reserved forest information management and forest resource information retrieval. For this, the user interface is developed by using Visual Basic 6.0 and MapObjects 2.1 of ESRI based on CBD(component based development) technology. The result of developing this system will not only perform constructing economical forest and better environment but also be the foundation of domestic spatial technology for forest resource management.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.1
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• pp.60-66
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• 2014

The aim of this study was to evaluate the antioxidant activity and protective effect of extracts from the stems and leaves of Chrysanthemum boreale (CBSL) on t-BHP induced oxidative stress in human liver cells (Chang cells). Antioxidant activities in the extracts were determined for various radical scavenging activities including ferric reducing antioxidant power, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, and oxygen radical absorbance capacity (ORAC). CBSL showed a very good scavenging effect of DPPH radical ($IC_{50}$ $0.009{\pm}0.002$ mg/mL), alkyl radical ($IC_{50}$ $0.004{\pm}0.001$ mg/mL), and hydroxyl radical ($IC_{50}$ $6.742{\pm}0.152$ mg/mL). CBSL also showed a strong antioxidant effect in the ORAC assay. In the MTT assay on human liver cells (Chang cells), the extracts showed protective effects by increasing cell viability, decreasing ROS, and restoring mitochondria membrane potential upon t-BHP induced oxidative stress. Our findings suggest that CBSL extracts are a potential therapeutic with protective antioxidant effects upon oxidative stress.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.5
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• pp.622-629
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• 2017

This study was carried out to investigate the effects of cold maceration and heat treatment prior to alcohol fermentation on the quality characteristics of wine made from Okrang grape cultivar. The results of this study show that alcohol contents of wines increased as fermentation period increased, whereas $^{\circ}Brix$ values decreased. The pH levels of wines ranged from 3.17 to 3.42 while the total acidities of wines ranged from 0.49~0.99%. The free sugar contents of wines tended to decrease as fermentation period increased. In particular, free sugar content of wine pre-treated at low temperature prior to alcohol fermentation slowly. Total polyphenol content of wine heat-treated prior to alcohol fermentation was higher than those of other wines and reached a maximum value after fermentation for 2 days. Campbell Early wine showed the strongest antioxidant effect, measured based on DPPH free radical scavenging activity, followed by Okrang wine pressing after fermentation. These results indicate that pre-treatment before wine fermentation greatly affects wine quality and biofunctional activities.

• The Transactions of the Korea Information Processing Society
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• v.4 no.3
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• pp.754-766
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• 1997

In this paper,we implement the Audit Trail Service Sydtem for the EDI Security.It has solved a law dispute between enterprises by informations that have generated by the EDI serice systrm.The audit trail service sys-tem implemented for EDI security satisfied the requirements of audit and the protocol of the security serive of X.435 and X.400.The EDI Security Audit System consists of the event discrimiator,the audit recirder,the audit archiver,and the provider of audit services .The event discriminator classified the reansmitted data from the EDI network ot audit sercices.The audit recorder constructs an index that has combined time information wiht audit unformations which are classified by the event discriminator.ZThe audit archiver performas the vacumming of added audit imformations by passing time by passing time.The audit provider is a module that carries out the audit trail servies by using stored audit informations. The audit provider suports audit servies,which are non-requdiation,proof and probe,controller of security,and accesing infrimation.The audit trail service system for EDI security constructs audit information by using index that is combining time imfromation,so it supports especially fast accesing audit information.

• Proceedings of KOSOMES biannual meeting
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• 2007.05a
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• pp.101-108
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• 2007

Tugboat has carried out the important role and service in the maritime industry construction, such as port and sea bridge construction, fairway dredging and sea reclamation etc. Furthermore, tugboat takes the largest portion in number of vessel at the domestic registry and barges as big as the general merchant vessel, which are getting specialized and larger, are in operation. In spite of the increase of marine accident under this situation, there has been no proper measure for the safe navigation of tugboat in the aspect of a nation. This paper aims to propose the measure for the safe navigation of tugboat according to the frequent marine accident of tugboat with sea bridge. Therefore, we show a example of the sailing schedule and operation checklist based on the analysis of statistics and precedent of marine accident and the investigation of the actual operation state of tugboat in the aspect of a contract of carriage and a personnel setup, which should be checked by the operator of tugboat, pass through sea bridge safely and propose the safe traffic supporting system based on electronic chart system to improve the safe navigation of tugboat.

• Food Science and Preservation
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• v.24 no.8
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• pp.1113-1121
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• 2017

The purpose of this study was to determine antioxidant and physiological activities of water and 70% ethanol extracts from Ligularia fischeri by extraction methods. The yield of water and ethanol extracts from Ligularia fischeri was 15.23% and 17.45%, respectively. The polyphenol and flavonoid contents of ethanol extracts of Ligularia fischeri (LEE) were $17.17{\pm}4.38mg/g$, $35.06{\pm}6.69mg/g$, respectively. The electron donating ability and SOD like activity, and ABTS radical ability of all Ligularia fischeri extracts were increased in a dose dependent manner, and those was the highest in LEE. Nitrite scavenging ability was higher in pH 1.2 than that in pH 3.0, and ethanol extract showed higher ability in pH 1.2 and 3.0. The xanthine oxidase and inhibition effect of all Ligularia fischeri extracts on tyrosinase were dose-dependently increased, and those was the highest in ethanol extracts of Ligularia fischeri. Reducing power was 1.2 at extract concentration $1,000{\mu}g/mL$ in water and ethanol extracts of Ligularia fischeri and the highest in water extract of Ligularia fischeri at concentration of $62.5-500{\mu}g/mL$. These results may contribute to development of processed food and health functional food with Ligularia fischeri.

• Korean Journal of Social Welfare
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• v.63 no.1
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• pp.5-28
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• 2011

This study, based on the research on the history of life, aimed to recompose and analyze into what life progressive structure the life experience by the mentally ill, after the onslaught of the disease, developed in a bid to understand the risk progress in the mentally ill's life, and to determine what contributed to the current stabilized recovery and adjustment. Five mentally ill persons participated in the study, and Sch$\ddot{u}$tze's narrative interview was used to gather data. The gathered data were analyzed according to Sch$\ddot{u}$tze's process structure of life. The interviewees' life experiences were chronologically organized as understood, and significant stories were recomposed that not only brought about changes but also helped overcome their disabilities in the process of treatment and rehabilitation after the onslaught of the disease. As a result, their experiences were recomposed into the stage of onslaught of the mental illness and confusion, and into the stage of intensive treatment and rehabilitation. The former was categorized into suppression by the disease, repetition and endurance of the painful life, and separation from their family and frustration. The latter was categorized into the rediscovery of self through social role change, others who helped realize the life potential, the expansion of mental health services in the community, obstacle to the integration of communities, re-integration of family relationships, re-analysis of experience of the disease through the examination of the life prior to onslaught of the disease, and expectation for the future. Also, these themes were comparatively examined so as to examine the crisis progress in the mentally ill's life after the onslaught of the disease, as well as the life transfer process through positive rehabilitation. Lastly, on the basis of these results, important areas of mental health services for the mentally ill were discussed.