• Journal of Food Hygiene and Safety
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• v.27 no.2
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• pp.146-151
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• 2012

In this study, effective gene extraction methods were compared to identify raw materials of processed meat products through molecular biological methods. Species specific primers were used to identify ingredients of processed foods and, as a sample, 13 kinds of processed meat products including beef, pork and chicken. According to the type of sample, 13 kinds of samples were classified into liquid type, source type and powder type. The samples were pre-treated (centrifugation) and (or) performed Whole Gene Amplification (WGA) kit for amplification of the extracted DNA. As a result, it was possible to identify the raw material of products through the centrifugation of sample 1 ml for liquid type of processed meat products. For source type of products after gene extraction, it was required to perform WGA for the identification of ingredients. For powder type products did not required any further pre-treatment and WGA. In this study, it was an opportunity to confirm the possibility of identification of raw material from the gene extraction of processed meat products and this method could be used to examine the authenticity of raw material of products.

• Analytical Science and Technology
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• v.29 no.2
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• pp.57-64
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• 2016

Many phthalic acid esters (PAEs), including DMP, DEP, DBP, BBP, and DEHP, as well as DEHA are widely used as plasticizers in plastics. An analytical method was developed and used to analyze these compounds at 41 industrial facilities. The coefficient of determination (R²) for each constructed curve was higher than 0.98. The method detection limit (MDL) values were 0.4–0.7 μg/L for PAEs and 0.6 μg/L for DEHA. In addition, the recovery rate was shown to be 77.0–92.3%, while the relative standard deviation was shown to be in the range of 5.8-10.5%. DMP (n = 3), DEP (n = 2), DBP (n = 2), BBP (n = 2), and DEHA (n = 3) were detected in the range of 2.2-11.1% in the influent. DEHP was a predominant compound and was detected at > MDL in both the influent (n = 16, 35.6%) and the effluent (n = 4, 10.0%) at a high removal efficiency (92–100%). The highest levels of residue in industrial wastewater influent were 137.4 μg/L of DEHP at plastic products manufacturing facility, 12.5 μg/L of DEHA at a chemical manufacturing facility, and 14.0 μg/L of DEP at an electronics facility. The highest concentration of effluent was 12.5 μg/L of DEHP at a chemical manufacturing facility, which indicated that the effluent was below the allowable concentration (800 μg/L). Therefore, the levels of PAEs and DEHA that are discharged into nearby streams could not influence the health of the ecosystem.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.11 no.4
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• pp.233-238
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• 1978

Rainbow trout were reared in a stainless steel aquarium from Nov. 11, 1977 to June 12, 1978, and the following results were obtained : 1. The volume of water was about $400\iota$ in a aquarium measuring $1m\;(Length)\times1m\;(Width)\times67cm(Height)$ and water depth 40 cm. Water was supplied for about 16 hours daily at a rate $3\iota/min$ and was drained through the conical settling part in the middle of the aquarium bottom. Filter tank was about $23cm(W)\times23cm(L)\times40cm(D)$ and contained pebbles 30 cm in depth. Water recirculation rate was at)out $1,030\iota/hr$, or 2.6 turn-over per hour. 2. During the first period (77 days), the trout grew from 88.3g to 229g in average, the total weight attaining 30.7kg. The food coefficient was 1.249, average daily increment 243.3g, average daily growth rate 1.245%, and the mortality was 2 smallest fish weighing 53 g, owing to unknown reason. During the second period (135 days), the trout grew from 239g to 555g in average, the total weight attaining 57.2 kg. The food coefficient was 1.447, average daily increment 279.8g, average daily growth rate $0.65\%$ and the mortality was 31 fish weighing 11,255 g, owing partly to miss-handling and partly to disease. 3. The feed consisting of fully domestic materials was prepared in this laboratory, and the feed conversion was not inferior to high protein commercial feed available in foreign countries. 4. The result of whole period for 212 days was 56.5 kg in gross increment, and based on this result, when $1\iota/min$ full day inflowing water available, the net production will become 28.25 kg. So, if a 5000kg production is planned, $180\iota/min$ or about $10.8m^3/hr$ be reauired, and the production in value frill become 15million won at local price at the expense of about 5.3 million won. From the result of this experiment, rainbow trout is feasible for commercial production in Korea with relatively small amount of well water and simplified water recirculation system.

• Korean Journal of Agricultural Science
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• v.25 no.1
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• pp.79-88
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• 1998

This study was performed in order to apply to effective breeding of Korean native cattle on the molecular genetic level obtained from PCR and nucleotide sequencing analysis of BoLA DRB3 exon2 that has important roles in host immune defence. Genomic DNA used in this study was prepared from the blood of Korean native cattle in Korean Native Cattle Improvement Center of National Livestock Cooperation. The results obtained from this study are summarized as follows: 1. Genomic DNA extracted from the blood of Korean native cattle was subjected to electrophoresis on 1.5% agarose gel. Major band was bigger than 12.2kb, indicating that genomic DNA was well prepared for PCR. Amplified products of 284bp fragments was obtained the amplification of BoLA DRB3 exon2 gene by PCR. 2. Cloning of BoLA DRB3 exon2 of Korean native cattle with pCR2.1 vector was conformed by 300bp fragment from recombinent plasmid that restricted with enzyme digestion of EcoRI. 3. Homology of BoLA DRB3 exon2 alleles of parent was 82.0% between sire's alleles and 90.1% between dam's alleles. 4. In pedigree analysis using BoLA DRB3 exon2 gene, sequencing result of BoLA DRB3 exon2 genes showed inheritance by Mendelian mode through the parents to their offspring. 5. Taking together those experimental results, pedigree was confirmed on the basis of sequencing for the alleles of parents and offspring. This knowledge by the molecular biological approach could be served for the improvement of Korean native cattle.

• Geophysics and Geophysical Exploration
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• v.9 no.1
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• pp.19-29
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• 2006

The first Japanese pilot-scale $CO_2$ sequestration project has been undertaken in an onshore saline aquifer, near Nagaoka in Niigata prefecture, and time-lapse well logs were carried out in observation wells to detect the arrival of injected $CO_2$ and to evaluate $CO_2$ saturation in the reservoir. $CO_2$ was injected into a thin permeable zone at the depth of 1110m at a rate of 20-40 tonnes per day. The total amount of injected $CO_2$ was 10400 tonnes, during the injection period from July 2003 to January 2005. The pilot-scale demonstration allowed an improved understanding of the $CO_2$ movement in a porous sandstone reservoir, by conducting time-lapse geophysical well logs at three observation wells. Comparison between neutron well logging before and after the insertion of fibreglass casing in observation well OB-2 showed good agreement within the target formation, and the higher concentration of shale volume in the reservoir results in a bigger difference between the two well logging results. $CO_2$ breakthrough was identified by induction, sonic, and neutron logs. By sonic logging, we confirmed P-wave velocity reduction that agreed fairly well with a laboratory measurement on drilled core samples from the Nagaoka site. We successfully matched the history changes of sonic P-wave velocity and estimated $CO_2$ saturation a(ter breakthrough in two observation wells out of three. The sonic-velocity history matching result suggested that the sweep efficiency was about 40%. Small effects of $CO_2$ saturation on resistivity resulted in small changes in induction logs when the reservoir was partially saturated. We also found that $CO_2$ saturation in the $CO_2$-bearing zone responded to suspension of $CO_2$ injection.

• Geophysics and Geophysical Exploration
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• v.9 no.1
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• pp.129-138
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• 2006

Helicopter-borne aeromagnetic surveys at two different times separated by three years were conducted to better understand the shallow subsurface structure of the Vulcano and Lipari volcanic complex, Aeolian Islands, southern Italy, and also to monitor the volcanic activity of the area. As there was no meaningful difference between the two magnetic datasets to imply an apparent change of the volcanic activity, the datasets were merged to produce an aeromagnetic map with wider coverage than was given by a single dataset. Apparent magnetisation intensity mapping was applied to terrain-corrected magnetic anomalies, and showed local magnetisation highs in and around Fossa Cone, suggesting heterogeneity of the cone. Magnetic modelling was conducted for three of those magnetisation highs. Each model implied the presence of concealed volcanic products overlain by pyroclastic rocks from the Fossa crater. The model for the Fossa crater area suggests a buried trachytic lava flow on the southern edge of the present crater. The magnetic model at Forgia Vecchia suggests that phreatic cones can be interpreted as resulting from a concealed eruptive centre, with thick latitic lavas that fill up Fossa Caldera. However, the distribution of lavas seems to be limited to a smaller area than was expected from drilling results. This can be explained partly by alteration of the lavas by intense hydrothermal activity, as seen at geothermal areas close to Porto Levante. The magnetic model at the north-eastern Fossa Cone implies that thick lavas accumulated as another eruption centre in the early stage of the activity of Fossa. Recent geoelectric surveys showed high-resistivity zones in the areas of the last two magnetic models.

• Tuberculosis and Respiratory Diseases
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• v.43 no.1
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• pp.30-37
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• 1996

Background: The extraction methods of DNA from clinical samples are the major obstacle to use the PCR(polymerase Chain Reaction) in routine labortary for early detection of M. tuberculosis. We tried to improve the extraction method of DNA from sputum for establishment of the PCR in routine labortary by reducing the possibility of cross contamination and performing it easily and safely. Methods: We used the $InstaGene^{TM}$ DNA extraction kit(BioRad Co.) using Chelex 100 ion exchange resin for preparation of DNA. We compared InstaGene method in 100 cases of sputum from proteinase K method which is known as the most commonly used method for DNA purification(Experiment 1). And we compared InstaGene method in 98 cases of sputum from Microwave method developed by a company in Korea(Experiment 2). In experiment 1,245bps of IS6110 were amplified and then 188bps were amplified by nested PCR. In experiment 2,536bps in primary PCR and 276bps in nested PCR were amplified and analysed by agarose gel electrophoresis and EtBr staining. Results: When we chose AFB smear, culture, or AFB smear and culture as a standard test, PCR had low specificity and positive predictive value in both experiments. The InstaGene method has higher value in sensitivity and negative predictive value significantly than proteinase K method. The InstaGene method and the Microwave methods were similar in sensitivity, specificity, positive predictive value and negative predictive value. Conclusion: Even though both methods had lower possibility of cross contamination, shorter time requirement, simplicity, and economic advantages than Proteinase K method, the InstaGene method was a little simpler than the Microwave method. Therefore, in terms of usefulness in clinical application, the Instagene method seems to be the most useful method in DNA extraction for detection of M. tuberculosis using PCR. The reliability of this method will be clarified by further studies with enough clinical samples.

• Korean journal of applied entomology
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• v.53 no.2
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• pp.149-156
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• 2014

The resistance levels of the green peach aphid, Myzus persicae (Sulzer), against 10 insecticides was checked and selected the applicable insecticide resistance markers. We conducted our study in 5 cabbage cultivation regions (Pyeongchang, Hongcheon, Bongwha, Muju, and Jeju) of Korea, over 3 successive years (2009-2011). We selected a multi-resistant (MR) strain from among the 5 field-collected populations. We analyzed esterase over-expression and mutation(s) in the target sites, by using native isoelectric focusing (IEF) and quantitative sequencing (QS). We detected esterase over-expression and StoF mutation in the acetylcholinesterase 1 gene (ace1) in all of the field-collected populations, including the MR strain. We did not detect the LtoF mutation, which is a well-known knockdown resistance (kdr) mutation in the para-type sodium channel gene (para), in the MR strain; however, the value of the MR strain for bifenthrin was 3,461-fold higher than that of the susceptible strain. Our results indicate that insecticide resistance is more effectively evaluated using molecular markers than by conducting a bioassay. The molecular markers StoF in ace1 and MtoL in para can easily be applied in diagnostic methods such as QS or PCR amplification of specific alleles (PASA). These methods may be extended to management of M. persicae resistance in the field.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.2
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• pp.220-228
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• 2017

Effects of extraction methods on reducing concentrations of pro-oxidants (total iron and protein) of salmon was determined. For development of the extraction process, the effectiveness of several extraction methods was determined and compared, including heat treatment (60, 80, and $100^{\circ}C$), ion exchange and carboxymethyl (CM)-cellulose column chromatography, and ultrafiltration (UF). Protein, total iron, and anserine contents of salmon extracts were 23.64 mg/mL, $16.20{\mu}g/mL$, and 5.47 mg/mL in non-heated extracts, 7.40 mg/mL, $2.32{\mu}g/mL$, and 5.20 mg/mL in heated extracts at $60^{\circ}C$, 7.64 mg/mL, $1.20{\mu}g/mL$, and 5.21 mg/mL at $80^{\circ}C$, and 7.04 mg/mL, $0.68{\mu}g/mL$, and 4.04 mg/mL at $100^{\circ}C$, respectively. Heating and UF decreased contents of protein and total iron, whereas only UF slightly decreased anserine content. Application of the primary ion exchange method increased the content of anserine up to 16%. Protein and total iron contents by the primary ion exchange method decreased by 70 and 98%, respectively. Secondary ion exchange (CM-cellulose) treatment after primary ion exchange and UF resulted in lower anserine content than the primary ion exchange method. However, the content of impurities (protein, total iron) was lower than in all other salmon extracts. Therefore, primary ion exchange, UF, and secondary ion exchange method were the best extraction processes in this study.

• Korean Journal of Poultry Science
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• v.34 no.3
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• pp.207-215
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• 2007

This work was carried out to assess the effect of microbial phytase (MP) on the performance, retention and excretion of phosphorus, and nutrients availability in broiler chicks. Two hundred and sixteen one day old Ross strains broiler chicks were used to investigate on performance for 5 weeks (earlier $(0{\sim}2wk)$, finisher $(3{\sim}5wk)$) and were divided 3 treatments, three cages per treatment and 12 birds per cage. Treatments are P0 (basal diet), P1 (microbial phytase 300 FTU/kg), and P2 (microbial phytase 600 FTU/kg). After 5 weeks, three diets containing 0, 300, 600 FTU/kg of microbial phytase were fed to 5-wk-old broilers to assess the digestibility. Twenty seven broilers were divided into 3 treatments (9 birds per treatment) to investigate on the absorption and retention of phosphorus and nutrient digestibility. Body weight gain and feed efficiency were highly significant at P1 and P2 treatments compared to those of P0 (P<0.05), however, feed intake was no significant difference among all treatments (P>0.05). The excretion of phosphorus decreased in P1 and P2 compared to P0 (P<0.05). The digestibility of nutrients and amino acid was high in P1 and P2 treatments compared to P0 (P<0.05). Ileal digestibility was lower about $1{\sim}2%$ than feces digestibility (P>0.05). Finally, microbial phytase influenced on the performance, the retention and excretion of phosphorus and ileal and feces digestibility in broilers.