• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.82-82
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• 2019

종자은행은 종자를 보존하는 가장 경제적이고 효율적인 보전방법이지만, 수종에 따라 저장특성의 차이가 커서 수종별 저장방법의 확립이 필요하다. 초저온저장 방법은 세포 분열, 대사 작용을 정지 상태로 유지하므로 현재까지 장기간 보존을 위한 비용이 효율적이며 안정적인 방법이다. 공시재료는 멸종위기 야생식물 등 22종을 대상으로 하였다. 액체질소에 7일간 종자를 침지한 후 $37^{\circ}C$ 항온수조에서 10분간 해동하였다. 휴면이 있는 수종은 파종 전 종자를 저온습윤 하였고, 저온습윤 처리 후에도 휴면타파 되지 않는 수종은 농황산, 지베렐린을 개별 또는 혼용으로 처리하였다. 발아 및 유묘 평가는 $25^{\circ}C/20^{\circ}C$(광12h/암12h)조건에서 진행하였으며, 매일 28일차까지 발아조사를 수행한 후 평가하였다. 진달래속의 꼬리진달래와 철쭉이 초저온 민감성을 보였으며, 오리나무속의 물오리나무, 사방오리는 부분 민감성을 보였다. 민산초나무는 지하부건물중이 유의하게 감소한 결과를 보였으며, 이외의 수종은 초저온 민감성을 보이지 않았으므로 장기보존을 위한 초저온 동결보존이 가능한 것으로 보였다.

• Journal of Bio-Environment Control
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• v.22 no.3
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• pp.241-247
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• 2013

For in vitro minimal-growth conservation of S. sarmentosum, the in vitro shoots with 10 mm length were cultured on Murashige and Skoog's media (MS) containing different levels of agar (0.8, 1.2, 1.6, 2%), Gelrite (0.4, 0.6, 0.8, 1%), ABA (0, 5, 10, $20mg{\cdot}L^{-1}$), and sucrose (2, 3, 6, and 9%) without subculture at $4^{\circ}C$ and $25^{\circ}C$. All media were supplemented with $0.2mg{\cdot}L^{-1}$ BA, agar and Gelrite media, with 5% sucrose, sucrose media, with 1.2% agar, and ABA media, with 5% sucrose and 1.2% agar, respectively. In vitro minimal-growth conservation in room-temperature ($25^{\circ}C$) was effective in the media containing with $10mg{\cdot}L^{-1}$ ABA or 1.6% agar, and the healthy plantlets could be preserved for 10 months without subculture. After 12 months at $4^{\circ}C$, survival rate was 100% in all media. The in vitro minimal-growth conservation in low temperature ($4^{\circ}C$) was effective in the media containing with $10mg{\cdot}L^{-1}$ ABA or 6% sucrose, and the healthy plantlets could be preserved over 18 months without subculture. Especially, long-term conservation using minimal growth of S. sarmentosum was much more efficient in the medium containing high level sucrose at $4^{\circ}C$ compared to others.

• Life and Agrochemicals
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• s.253
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• pp.42-43
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• 2009

지금 세계는 인구증가로 인한 시량부족이라는 커다란 위기단계에 직면해있다. 현재 70억, 2050년까지 90억 이상의 사람들에게 충분한 식량공급과 식품품질을 확보하는 것은 국제사회가 처한 매우 중대한 도전이다. 급격히 증가하는 식량 수요를 만족시키기 위한 대부분의 해결책들은 필수적으로 농산물의 생산량 증가에 기반하고 있지만, 이제는 지구 환경을 보호하는 지속농업을 유지하면서 세계 식량생산량의 25%에 해당되는 수확 후 손실을 최소화하는 방향으로 접근하는 것에 초점을 맞추어야 할 것이다. 저온저장은 수확 후 손실을 줄이는데 핵심적인 역할을 해오고 있다. 현재 방사선, 건조, 염장, 고압가공, 통조림 등 많은 수확후 보존기술들이 부패하기 쉬운 음식에 적용되고 있기는 하나, 이들 어떤 기술도 저온저장 기술처럼 소비자가 요구하는 생산물 저장수명 연장과동시에 초기의 물리적, 화학적, 영양학적, 감촉 특성을 유지시켜 주지는 않는다. 따라서 저온저장기술의 이용 증대만이 식품의 양과 질 측면에서 보다나은 전 세계인의 영양공급을 확보할 수 있게 할 것이다.

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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• v.33 no.7
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• pp.21-25
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• 2004

생물재료인 식품의 성분 중에서 물은 식품의 주요기능인 영양기능(1차기능), 기호기능(2차기능), 생체 조절기능(3차기능)의 관점에서 무시되는 경우가 많으나 식품의 수송, 보존, 가공 공정에서 직접적으로 제어해야 하는 대상이므로 가장 먼저 고려해야 할 존재이다. 일반적으로 식품의 보존 공정에서 수분활성은 여러 가지의 화학반응, 효소반응, 미생물의 생육속도 등에 영향을 미치게 된다. 특히, 미생물 생육을 위해서는 높은 수분 활성을 필요로 하므로, 수분활성을 제어함으로써 미생물의 제어가 가능하다. 따라서 저온의 이용도 기본적으로는 미생물 및 수분의 활성제어를 위한 공정이라고 말할 수 있다. 최근에는 수분의 활성을 억제하기 위하여 어류의 냉각공정에 사용되는 빙수를 해수나 수도수가 아닌 해양 심층수를 이용함으로써 미생물의 활성을 제어하는 연구가 진행 중이다. (중략)

• Microbiology and Biotechnology Letters
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• v.16 no.6
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• pp.517-521
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• 1988

This study focused on the psychrotrophic post-pasteurization contamination of fluid milk pro-ducts which were processed by HTST system. Pasteurized line samples and container samples of each fluid milk product (whole milk and skim milk) were taken in a large fluid milk plant. tine samples were collected through nine and five different sampling locations for whole milk and skim milk products, respectively. Each sample was subjected to preliminary incubation (PI) at 21$^{\circ}C$ for 16h followed by standard plate count (SPC) and crystal violet tetrazolium agar count (CVT). Flavor, SPC, and psychrotrophic bacteria count (PBC) were determined after 7 d at 7.2$^{\circ}C$. In addition, ten sequential container samples (packaged in 1000ml paperboard containers) were taken from a filler at the beginning of each product run. These samples were used for PI followed by SPC and CVT. In addition, flavor evaluations, SPC and PBC tests were conducted after 7,10, and 14 d at 7.2$^{\circ}C$. The mean PI-CVT values for the line samples showed differences depending on the location. There was major contamination between pasteurized storage tank and the filler. The PI-CVT counts for each container sample were negatively correlated with flayer scores at 10 and 140. There were good correlations among PI-CVT values of line samples and the percentage of total container samples with acceptable flavor after 10d.

• Korean Journal of Food Science and Technology
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• v.23 no.3
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• pp.379-387
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• 1991

The effects of organic acids (acetic, citric and lactic acids) treatment on microbial spoilage of chilled beef were studied during aerobic storage at $4^{\circ}C$ for 11 days. The organic acids had definite effects on the delay of the development of off-odor and slime of chilled beef. When chilled beef slices were treated with 1, 2, 3 and 4% of organic acids, off-odor was developed 1, 2, 3 and 5 days later than control, respectively, regardless of the kinds of organic acid. The slime was produced two days after the day of off-odor development in $1{\sim}3%$ organic acid-treated chilled beef, but no slime was produced on chilled beef treated with 4% organic acid. The off-odor was detected organoleptically when pH and number of microorganisms of chilled beef were, $5.6{\sim}5.8\;and\;0.8{\times}10^7{\sim}1.8{\times}10^7\;cell/cm^2$, respectively, and slime was observed when pH and number of microorganisms of chilled beef were $5.9{\sim}6.2\;and\;2.0{\times}10^7{\sim}6.0{\times}10^7\;cell/cm^2$, respectively, in control and treated groups.

• The Korean Journal of Mycology
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• v.26 no.1 s.84
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• pp.91-96
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• 1998

Optimal conditions for the preservation of mushroom cultures in sterile distilled water were investigated. Mycelial disks from 12 mushroom cultures were preserved at room temperature, $5^{\circ}C,\;-20^{\circ}C$ and $-75^{\circ}C$ using sterile water and 6 cryoprotectants for 12 months. The maximal viability of mycelial disks was observed at room temperature and $5^{\circ}C$ with the value of 91.7%. At $-75^{\circ}C$, the viability was highest in polyethylene glycol (PEG) and lowest in 10% dimethyl sulfoxide (DMSO). The average mycelial growth was highest for disks preserved at $-75^{\circ}C$ in PEG. PEG was the most suitable for the preservation at $-75^{\circ}C$, although this chemical has rarely been used as a cryoprotectant in fungal culture preservation. The laccase isozyme patterns of the preserved isolate of H. ferrugineum were not changed during preservation at $5^{\circ}C$ in sterile distilled water as compared to that of slant culture.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.59-59
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• 2020

수국 '베레나 그린'의 줄기하단의 엽을 제거하고 5엽 상태에서 수돗물과 0.1% 크리잘 RVB에 각각 담가 4℃에서 24시간 물올림을 하고 물올림을 하고, 줄기를 60~70cm 길이로 끝을 대각선으로 절단하여 물올림용액을 채운 물 대롱을 끼운 다음 꽃을 보호하기 위한 비닐 슬리브를 씌워 유통용 종이상자에 담아 5±1℃ 냉장차로 국립원예특작과학원으로 운반하였으며, 절화수명 및 관련 특성분석을 위해 평가실로 옮기기 전까지 유통용 종이상자에 담긴 상태로 저온저장고(5±1℃)에 보관하였다. 보존용액에 따른 절화특성 조사를 위해 절화를 평가실에 전시하기 전에 절화의 줄기길이를 40cm로 맞춰 절단한 뒤에 보존용액을 800 mL 채운 플라스틱 화병에 꽃아 절화 화관끼리 서로 닿지 않도록 배치하였으며 22±2℃ 실내에서 절화수명을 조사하였다. 보존용액으로 4% 차아염소산나트륨 용액, 1% sucrose + 250 mg/L 8-hydroxquinoline + 100 mg/L citric acid로 이루어진 용액(HQ) 그리고 시중에서 수국 절화 보존제로 판매되는 크리잘 프로 II, III와 크리잘 clear 0.5% 용액을 사용하였다. 수돗물에 물올림하였을 때 보존용액인 0.5% 크리잘 프로 II에서는 33.8일, 0.5% 크리잘 프로 III에서는 27.7~33.5일, 크리잘 클리어는 33.7일, 차아염소산나트륨과 수돗물은 각각 26.2, 28.8일이었고, HQ 용액은 49.0일로 크리잘보다 15일, 수돗물보다 20일 절화수명이 연장되었다. 수국 '베레나 그린'의 보존용액으로 1% sucrose + 250 mg/L 8-hydroxquinoline + 100 mg/L citric acid를 사용하는 것이 관상기간을 연장하는 데 효과적이었다.

• Journal of Conservation Science
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• v.30 no.3
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• pp.299-306
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• 2014

The possibility of a low-temperature argon plasma treatment as a mean of restoration technology for contaminated invaluable archive materials and artefacts, and evidencing documents was investigated along with an oxygen plasma treatment for comparison. For this purpose, the degree of color changes, ${\Delta}E^*ab$, and surface morphological changes due to plasma treatments as an evaluation of removal performance of artificial contaminants such as brilliant green dye and carbon deposit on cellulose acetate and plain paper as matrices, respectively, were measured and analyzed using a spectrophotometer and a field emission scanning electron microscope. Compared to the argon plasma treatment with sputtering characteristic, that of the oxygen plasma with characteristic of an oxidation reaction has shown superior results in removing the contaminants; the oxygen plasma has proven to damage the matrices significantly due to its oxidative characteristic, and post-plasma reactions has anticipated to be also detrimental to the surfaces, whereas, the problems caused by the counterpart has resulted in being negligible and rather has thought to be an appropriate mean for delicate restoration and/or removal operations of contaminants.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.16 no.3
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• pp.216-224
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• 1983

This work was undertaken to obtain some characteristics of proteolytic enzyme of marine gastropods such as sea hare, Aplysia kurodai, top shell, Turbo cornutus, and abalone, Haliotis discus hannai. An influence of pH, temperature and some chemicals on proteolytic activity of the crude enzyme extracted from digestive tract of the samples was taken into account and the stability of the enzyme during the storage at low temperature was also discussed. In comparison of the activities of the crude enzyme from the samples to the optimum conditions, it was characterized that abalone has twice or the more times higher activities than the other two species of the gastropoda in the acid and weak acid region, while, in the alkaline region, sea hare has six or the more times higher acitivities than the other two species. The proteolytic activity was facilitated by $Mn^{2+}$, some reducing agents, EDTA and DTT, and inhibited by $Hg^{2+}$ and SDS, but the other chemicals were not significantly affected to the activity. The low temperature storage of the enzymes of sea hare and top shell at $0^{\circ}C\;or\;-20^{\circ}C$ was not affected to the enzymic activity under the optimum pH condition except in the alkaline region. On the other hand, the low temperature storage was brought about no significant effect on the activity of the enzymes extracted from abalone under the optimum condition of the weak acid region, but apparently influenced to the activity under the optimum condition of the acid and alkaline region.