Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)
- Volume 16 Issue 3
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- Pages.216-224
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- 1983
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- 0374-8111(pISSN)
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- 2287-8815(eISSN)
Proteolytic Activity of the Crude Enzyme Extracted from the Digestive Tract of Marine Gastropods
해산복족류의 소화관조직중에 분포하는 단백질분해효소의 활성
- CHO Deuk Moon (Department of Nutrition and Food Science, National Fisheries University of Pusan) ;
- PYEUN Jae Hyeung (Department of Nutrition and Food Science, National Fisheries University of Pusan) ;
- BYUN Dae Seok (Department of Nutrition and Food Science, National Fisheries University of Pusan) ;
- KIM Chang Yang (Department of Food Science and Technology, National Fisheries University of Pusan)
- Published : 1983.07.01
Abstract
This work was undertaken to obtain some characteristics of proteolytic enzyme of marine gastropods such as sea hare, Aplysia kurodai, top shell, Turbo cornutus, and abalone, Haliotis discus hannai. An influence of pH, temperature and some chemicals on proteolytic activity of the crude enzyme extracted from digestive tract of the samples was taken into account and the stability of the enzyme during the storage at low temperature was also discussed. In comparison of the activities of the crude enzyme from the samples to the optimum conditions, it was characterized that abalone has twice or the more times higher activities than the other two species of the gastropoda in the acid and weak acid region, while, in the alkaline region, sea hare has six or the more times higher acitivities than the other two species. The proteolytic activity was facilitated by
해산복족류의 소화관조직에 분포하는 단백질분해효소의 활성에 미치는 pH, 온도, 화학약제, 저온보존 등의 영향을 밝히므로서 그 생리 생태학적 관련 지식을 얻고, 나아가서 그 정제를 위한 몇가지 기본적인 성질을 구명코자 본 연구를 시도하였다. 실험 결과를 요약하면 다음과 같다. 1. 군소, 소라, 전복의 소화관조직에서 추출된 단백질분해효소의 활성최적조건에서의 활성을 보면, 산성역과 약산성역에서는 전복이 다른 두 복족류에 비하여 2배 혹은 그 이상 높았으며, 알카리성역에서는 군소가 다른 두 복족류이 비하여 6배 혹은 그 이상 높은 특징을 보였다. 2. 화학약제가 효소의 활성이 미치는 영향을 종합하여 보면, 대체로 금속이온중의
Keywords