• Title/Summary/Keyword: 재용해

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Controlled Lysis of Lipase-Producing Recombinant E. coli by Phage Induction (Lipase를 생산하는 재조합 대장균의 phage에 의한 조절적 용균)

  • 문윤희;구윤모
    • KSBB Journal
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    • v.10 no.5
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    • pp.575-581
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    • 1995
  • A plasmid pTTY2, containing the lipase-producing gene, was used to transform an E. coli phage lysogen, P90c/$\phi$434, into the lipase-producing lysogen, P90c/$\phi$434/pTTY2. After the overproduction of lipase by the isopropylthio-${\beta}$-D-galactoside induction, the prophage $\phi$434 in the chromosome of the host cell was induced by the milomycin C addition or ultraviolet irradiation to lyse the host cell. The optimum operating conditions, such as the isopropylthio-${\beta}$-D-galactoside induction period and the phage induction timing, were sought for the efficient cell lysis in the same fermenter. Effective cell lysis occurred at the earlier exponential growth phase with the isopropylthio-${\beta}$-D-galactoside induction period of 1 hour. The amount of the lipase production was qualitatively measured by the halo size in Luria-Bertani agar medium containing tributyrin and Rhodamine B plate.

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Particle Dissolution Effects on Soluble Geo-Mixtures (용해성 지반혼합재의 입자 용해 영향)

  • Tran, M. Khoa;Cho, Se-Hyun;Byun, Yong-Hoon;Shin, Ho-Sung;Lee, Jong-Sub
    • Journal of the Korean GEO-environmental Society
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    • v.12 no.12
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    • pp.5-12
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    • 2011
  • Macro- and micro-behaviors of soluble granular media during dissolution process is investigated by numerical analysis. Soluble media are represented by assemblies of non-soluble particles and soluble particles with the different soluble particles contents. Dissolutions of particles are implemented by reducing sizes of soluble particles. The numerical simulations results exhibit that the vertical displacements increase to certain times and become constant while the porosities still increase until no soluble particles are present. However, the porosities and vertical displacements increase with the increase of soluble particles content. The microscopic views show that the fabrics of media change during dissolution process until the certain times, the higher soluble particles contents, and the larger change in fabric.

Characteristic Evaluation of FA-Based Geopolymer with PLA Fiber (PLA 섬유를 가진 다공성 플라이애시 기반 지오폴리머의 특성 평가)

  • Kwon, Seung-Jun;Hwang, Sang-Hyeon;Cho, Young-Keun;Kim, Tae-Sang;Moon, Eun-Jin
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.7 no.3
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    • pp.187-193
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    • 2019
  • Regarding physical absorption mechanism for fine particles(Dust), internal pore-bridging is a major parameter in porous media. In this paper, internal bridging pore system is invented through FA-based geopolymer and incorporated PLA (Polylactic Acid) fiber with biodegradability. With various mix proportions, compressive strength over 20MPa is obtained but PLA is little dissolved in the condition of NaOH 5mole and $30^{\circ}C$ of temperature, which was found that temperature rising accelerates PLA solubility. Within 24hours, beads type PLA is completely dissolved under $90{\sim}130^{\circ}C$ and NaOH 5~12mole of alkali. In room condition, geo-polymerization is limitedly occurs so that the internal pore after PLA dissolution is thought to be effective to absorption and storage of fine particles.

Dissolution Monitoring of Geo-Soluble Mixtures (지반 소실 혼합재의 용해과정 모니터링)

  • Truong, Q. Hung;Byun, Yong-Hoon;Eom, Yong-Hun;Sim, Young-Jong;Lee, Jong-Sub
    • Journal of the Korean Geotechnical Society
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    • v.25 no.10
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    • pp.111-122
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    • 2009
  • Dissolution of some of geo-materials may yield the loss of the soil strength and the settlement of earth structures. The goal of this study is to monitor the several physical behaviors of soluble mixtures during dissolution. Sand-salt mixtures are used to monitor the meso to macro response including the settlements and shear waves. The mixtures of photoelastic and ice disks are used to monitor micro to meso behavior of soluble mixture including the void ratio, force chain, coordination number and horizontal force changes. In the sand-salt mixtures, shear waves are measured by using bender elements in conventional oedometer cells. In the photoelastic disk - ice disk mixtures, micro to meso response are measured by digital images and load cells. The shear wave velocity decreases at the initial stage of the dissolution, and then increases and approaches to asymptotic value. The larger dissoluble particle and the more random packing produces the severe horizontal fore change. After dissolution, the void increases and the coordination number decreases. This study demonstrates that the particle level behavior such as the changes of the force chain, void ratio, and coordination number affects the global behavior such as the change of the shear wave velocity and horizontal force of the system.

Effect of Distribution and Shape of Cr-Carbide Precipitates on the Caustic stress Corrosion cracking for Ni-Based Alloy 600 (크롬계 탄화물의 분포와 형태가 니켈계 합금 600의 염기응력부식에 미치는 영향)

  • Kim, Seon-Jae;Choe, Jong-Ho;Seong, Jin-Gyeong;Kim, U-Gon;Park, Sun-Dong;Lee, Chang-Gyu;Jeong, Yong-Hwan;Guk, Il-Hyeon
    • Korean Journal of Materials Research
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    • v.8 no.8
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    • pp.766-774
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    • 1998
  • 32$0^{\circ}C$, 40%NaOH 용액의 autoclave에서 약 300wppm의 탄소를 함유하고 있는 15Cr-9Fe-balanced Ni 합금 판상시편에 대해 응력부식 저항성을 조사하였다. 부식시편은 $700^{\circ}C$, 100시간 동안의 열처리로 합금내부에 석출될 수 있는 가능한 한 많은 양의 크롬계 탄화물을 석출시킨 후, 다시 재용해에 의해 크롬계 탄화물의 형태를 조절하는 $800^{\circ}C$-$950^{\circ}C$범위의 최종열처리를 시행하고 급냉시킨 다음 U-자형으로 응력을 가하여 준비되었다. 최종열처리 온도가 올라감에 따라 시편들의 입계응력부식균열(IGSCC ) 전파속도는 $900^{\circ}C$까지는 거의 직선적으로 증가하다가 $950^{\circ}C$에서는 $700^{\circ}C$에서 얻은 값보다도 더 낮게 감소하였다. 즉, 크롬계 탄화물이 재용해되어 그 밀도가 감소함에 따라 IGSCC저항성이 감소하다가 완전히 재용해된 $950^{\circ}C$ 열처리 조건에서 오히겨 가장 큰 IGSCC 저항성을 나타내었다. 이와같은 최조열처리 온도에 따른 니켈계 합금 600의 부식거동은 입계에 존재하는 크롬계탄화물의 형태변화 때문이 아니라 입계에서 탄소-크롬계 탄화물-크롬간의 상평형에 의해 이루어지는 탄소의 입계편석량이 크롬계탄화물이 존재할 때에는 열처리 온도에 따라 증가하다가 그것이 완전히 재용해 되었을 때 가장 낮아지기 때문인 것으로 생각된다.

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The annihilation of the flow pattern defects in CZ-silicon crystal by high temperature heat treatment (고온 열처리에 의한 결정결함의 재용해)

  • 서지욱;김영관
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.11 no.3
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    • pp.89-95
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    • 2001
  • The CZ-silicon crystal was annealed at $1350^{\circ}C$ to dissolve the vacancy type grown-in defects. A this temperature, the equilibrium concentration of the oxygen in the silicon crystal is around $1.7{\times}10^{18}$ which induces the oxygen undersaturation in the silicon crystal. This situation results in the faster dissolution of the grown-in defects in the bulk of the silicon wafer than near the surface. This indicates the possibility that the presence of the higher concentration of silicon interstitial hinders the dissolution of the grown-in defects, which were known to compose of the vacancy clusters with surrounding silicon oxide film. This expectation was confirmed by the observation that the slower dissolution of the grown-in defects near the surface of the silicon wafer in the oxygen atmosphere than in the argon atmosphere. This result is quite opposite to the previous argument hat presence of the excess silicon interstitial leads to faster dissolution of the vacancy type defects.

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Rapid and Low-Energy Melting of Cast Iron using Small Scrap Steel as a Charge Material - Part I. Application of Small Scrap Steel in Medium-Frequency Induction Melting Furnace and Usage Characteristics (소형 고철 장입재를 활용한 신속 저에너지 주철 용해 - Part I. 중주파 용해로 적용 및 사용 측면 특징)

  • Lee, Sang-Hwan
    • Journal of Korea Foundry Society
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    • v.41 no.1
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    • pp.11-15
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    • 2021
  • Scrap steel used as a charge material in melting process of cast iron foundry is mostly press scrap. The press scrap usually causes several problems including energy loss, workability, safety, and so on. By charging the shredded scrap instead of the press scrap, it is expected that the above-mentioned problems can be solved. In this study, the power basic units to produce cast iron by using the press and shredded scrap are compared in 3t/h medium-frequency induction melting furnace. Charging the shredded scrap instead of the press scrap was confirmed that the power basic unit is improved by about 15%. The characteristics and restrictions of the usage according to the shape and size of scrap steel were considered.