• Title/Summary/Keyword: 재생율

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Cryopreservation of Mulberry Winter Buds in Liquid Nitrogen (뽕나무 동아의 액체질소내 동결보존)

  • Bang, Hye-Seon;Lee, Wan-Ju;An, Yeong-Hui
    • Journal of Sericultural and Entomological Science
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    • v.37 no.2
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    • pp.186-187
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    • 1995
  • 동결보존에서 가장 재생율이 높은 품종은 조직배양에서 발근력이 뛰어난 것으로 알려져 있는 신광뽕으로 최고 92.0%의 재생율을 보였으나, 개량, 용천 및 검설뽕에서는 재생을 보이지 않았다. 또, 건조과정을 거치지 않은 것이나, 건조과정과 예비동결 과정을 거치지 않고, 동결방어제만을 사용한 것의 재생은 전혀 확인할 수 없었다. 금후, 동결보존한 동아의 재생에서 돌연변이 유무는 RAPD로 확인될 수 있을 것이다.

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Experimental Study on the Properties of Recycled Concrete using Recycled Coarse Aggregates and Steel Slag Fine Aggregates (재생 굵은골재와 제강슬래그 잔골재를 사용한 재생 콘크리트의 특성에 관한 실험적 연구)

  • Lee, Jaesung;Na, Okpin
    • Resources Recycling
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    • v.24 no.5
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    • pp.63-71
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    • 2015
  • The purpose of this study is to investigate the optimum replacement rate and material properties of eco-friendly recycled concrete using recycled coarse aggregates and rapid-chilled steel slag fine aggregates. The replacement rate of recycled coarse aggregates was increased from 30% to 50% of total volume of coarse aggregates and the rapid-chilled steel slag aggregates were substituted for 10% to 50% of total volume of fine aggregates. As a result, the increment of recycled coarse aggregates in concrete caused the reduction of the compressive strength. On the other hand, as increasing the replacement ratio of rapid chilled steel slag aggregates, the compressive strength was enhanced. Furthermore, the optimum use of rapid chilled steel slag aggregates was suggested up to 20~30% of fine aggregates and the use of it could be helpful to expand the replacement rate of recycled aggregates.

Freezing and Thawing Properties of Concrete Using Recycled Coarse Aggregate (재생굵은골재를 사용한 콘크리트의 동결융해 특성)

  • Sung, Chan-Yong;Youn, Joon-No;Kim, Young-Ik;Im, Sang-Hyuk;Jung, Duck-Hyun
    • Korean Journal of Agricultural Science
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    • v.30 no.2
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    • pp.154-163
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    • 2003
  • This study was performed to examine the freezing and thawing properties of the high strength concrete using recycled coarse aggregate. The recycled coarse aggregate was replaced by 0%, 25%, 50%, 75% and 100% of natural crushed aggregate. The compressive strength of the concrete used recycled coarse aggregate was shown in more than $400kgf/cm^2$ at the curing age 28 days. The weight loss ratio by freezing and thawing was shown in less than 1% at all mix type. The pulse velocity and relative dynamic modulus were decreased with increasing the freezing and thawing cycles. Also, durability factor for the freezing and thawing were decreased with increasing the content of recycled coarse aggregate. But, recycled concrete replaced with recycled coarse aggregate 100% was shown in more than 60 by durability factor in freezing and thawing of 300cycles Accordingly, these recycled coarse aggregate can be used for high strength concrete.

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Cryopreservation of Chrysanthemum morifolium cv. 'White ND' Shoot Tips using Encapsulation-Dehydration-Vitrification Method (캡슐화-탈수화-유리화에 의한 국화 품종 '화이트 엔디' 신초의 초저온 동결보존)

  • Jeon, Su Min;Kim, Chang Kil
    • Current Research on Agriculture and Life Sciences
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    • v.32 no.2
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    • pp.99-103
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    • 2014
  • This study investigated the effects of cryopreserving Chrysanthemum morifolium cv. 'White ND' shoot tips for eliminating viroids. As a result, smaller shoot tips (2-3 LP, 1mm) showed a better survival and regrowth than larger shoot tips (4-5 LP, 1.5mm). The most effective vitrification solution for survival and regrowth was PVS3, which induced a high survival rate after 60 minutes of incubation. For a high efficiency, the best pre-treatment condition for vitrification was incubation in 88 mM sucrose for 24 h, 0.3M sucrose for 16 h, 0.5 M sucrose for 6 h, and 0.7 M sucrose for 3 h, in a descending order. The ploidy levels were the same in the mother plants and following cryopreservation, which confirmed the absence of any gene mutation.

Freezing-and-Thawing Resistance and Strain Characteristics of Recycled Concrete (재생콘크리트의 동결융해저항성과 변형특성)

  • 김광우;이봉학;도영수
    • Magazine of the Korea Concrete Institute
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    • v.4 no.4
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    • pp.115-122
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    • 1992
  • 폐쇄 폐콘크리트를 재활용한 재생콘크리트의 강도특성을 천연골재를 사용한 일반콘크리트와 비교하였다. 동결융해 처리수의 압축강도와 3점휨 재하시험하의 변형율을 측정하였다. 재생콘크리트는 동결융해 처리 후 압축강도 보존율이 더 높았다. 재생콘크리트는 또한 높은 변형율과 처짐에 민감함을 보였으나 파괴와 관련된 다른 성질들은 일반콘크리트와 유사하거나 더 좋은 것으로 나타났다. 그러므로 폐콘크리트를 구조용 콘크리트 제조에 재 사용이 가능할 것으로 보여진다. 그러나 실제 사용을 위하여는 콘크리트에 있어서 중요한 성질인 압축강도가 더 증진되어야 하며 최대 변형율도 보다 자세히 점검되어야 한다.

Study on the development of body marking method of Dark-banded rockfish Sebastes inermis (배지느러미, 아가미 뚜껑 절단에 의한 볼락 어체 표식방법 연구)

  • 명정구;노충환;조선형;박용주;이순길;홍경표;김창훈;안재만
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.140-140
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    • 2003
  • 볼락을 대상으로 125일간의 어체 표식실험 결과 50% 절단 실험구에서 88.8~100%(평균 95.1%)의 재생율을 나타내었으며, 100% 절단 실험구에서는 55.4~92.5%(평균 76.4%), 요대골을 뽑은 실험구에서는 0~68.2%(평균 17.1%)의 재생율을 나타내었다. 배지느러미 절단법은 단기간 실험에 사용 가능하며 장기적인 실험에는 아가미 뚜껑 절단법이 가장 효율적인 것으로 나타났다.

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A Study on the Properties of Recycled Concrete Using Recycled Fine Aggregates with different Removal formulas of Powder In Aggregate (미분 제거방식이 다른 2종의 재생 잔골재가 콘크리트외 특성에 미치는 영향)

  • Lee Mun-Hwan;Lee Sea-Hyun;Shim Jong-Woo
    • Journal of the Korea Concrete Institute
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    • v.17 no.1 s.85
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    • pp.95-104
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    • 2005
  • The research conducted to study the potential practicability of recycled aggregate concrete by analyzing the characteristics of concretes made of recycled quality aggregates produced by wet and dry process has found the following results. The air content of recycled aggregate concrete increased with increase of the substitut on rate due to mortar included while producing recycled aggregates. However, the concretes with aggregate produced by dry process had relatively low rate of increase in air content. The slump showed generally decreasing trend as the substitution rate of recycled aggregate increased regardless of the wet or dry process. It was assumed that the mortar particles remained in recycled aggregate absorbed the surplus hydration in concrete and decreased fluidity The compressive strength generally decreased as the substitution rate of recycled aggregate increased, however there was an increasing trend as well due to decreasing effect of water-cement ratio when the substitution rate of recycled aggregate reached 25, 50% after mix. This phenomena also appeared in early age, which meant that recycled aggregate concrete should not be retarded in setting when applied in the field. The tensile strength also reached the maximum when wet or dry recycled aggregate replaced with 25%. To conclude, recycled aggregates for concrete produced by wet or dry process are expected to demonstrate essential characteristics of concrete without significant decline in physical or dynamic quality when the substitution rate is below 25% although there are variations subject to water-cement ratio. However, slight differences are expected due to types of recycled aggregate and physical quality.

Elimination of Discontinuity Phenomenon for Repeated Play of Finite DTV Stream (유한 DTV 스트림의 반복 재생시 불연속 현상 제거)

  • Han, Chan-Ho;Sohng, Kyu-Ik
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.27 no.10A
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    • pp.951-961
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    • 2002
  • In general, there is discontinuity phenomenon like a black screen and an irregular sound for repeated play of a finite digital stream. In this paper, for repeated play we analyze the relation between source and stream time causes this phenomenon. We obtain the time relation between video frame rate, audio frame rate, and TS packet transmission rate to eliminate this phenomenon. Using this time relation, we propose a new generation method of elementary stream (ES) and transport stream (TS) to eliminate discontinuity phenomenon. The test results of the generate ES and TS using the proposed method show that the discontinuity phenomenon can be eliminated for repeated play of a finite proposed stream.

The Spheroplast Formation and Regeneration of Pseudomonas spp. (Pseudomonas spp.의 원형질체 생성과 재생)

  • 이주실;이영록
    • Korean Journal of Microbiology
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    • v.24 no.1
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    • pp.24-31
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    • 1986
  • The optimal conditions for the formation and the regeneration of Pseudomonas spheroplast were measured. Pseudomonas spp. cells were transformed to spheroplasts from 99.0% to 99.9% by treatment of $100{\mu}g/ml$ lysozyme and 10mM EDTA at room temperature. The optimal pH for the spheroplast formation was pH 8.0. Magnecium chloride, calcium chloride and streptomycin were effective on the stabilization of Pseudomonas spheroplast, while $Mg^+\;and\;Na^+$ ions were effective on the formation of Pseudonomas spheroplast. Rich Regeneration Medium was used for the regeneration of Pseudonomas spheroplast. To improve regeneration frequency, Bovin Serum Albumine and cationic ions were added to the spheroplast dilution beffer and regeneration environment respectively. Treatment of 20mM calcium chloride in ehr Rich Regeneration Medium could improve the yield of regenerants as much as 28-fold. Treatment of 1% Bovin Serum Albumine in the spgeroplast formation and dilution buffer increased the yield of regenerants to 10-fold. Also, the regeneration frequency was improved to 14-fold shen Rich Regeneration Meidum containing 0.5% Gelatin was used for regeneration as well as 1% Bovin Serum Albumine.

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Evaluation of Application of High Quality Recycled Fine Aggregate Manufacturing System by the Drying Specific Gravity Separation Method (건식비중분리법에 의한 고품질 재생잔골재 제조생산 시스템의 적용성 평가)

  • Kim Moo-Han;Kim Jae-Hwan;Kim Yong-Ro;Na Chul-Sung
    • Journal of the Korea Concrete Institute
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    • v.17 no.1 s.85
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    • pp.69-76
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    • 2005
  • In this study, the high qualify recycled fine aggregate manufacturing system by the drying specific gravity separation method was evaluated. For the evaluation of the performance of the recycled aggregate, the engineering properties and durability of recycled aggregate has been tested. From the test results, the quality of recycled fing aggregates was improved by high quality recycled fine aggregate manufacturing system and satisfied with the quality standards of KS and JASS 5. Also, compressive and tensile strengths of recycled concrete show no critical difference caused by recycled fine aggregate replacement ratio. However, durability such as carbonation depth chloride ion penetration depth and drying shrinkage shows more deterioration than the concrete without recycled fine aggregate