• Restorative Dentistry and Endodontics
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• v.36 no.1
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• pp.26-36
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• 2011

Objectives: In the present study, three kinds of tissues cells (pulp, gingiva, and periodontal ligament) were investigated if those cells express MMP and TIMP when they were stimulated with neuropeptides (substance P, CGRP) or proinflammatory cytokine, TNF-$\alpha$. Materials and Methods: The cells cultured from human dental pulp (PF), gingiva (GF) and periodontal ligament were (PDLF) stimulated with Mock, SP, TNF-$\alpha$, and CGRP for 24 hrs and 48 hrs. for an RNase protection assay and Enzyme Linked Immunosorbent Assay. Cells (PF, GF and PDLF) seeded in 100 mm culture dish were stimulated with SP ($10^{-5}$, $10^{-8}\;M$) or only with medium (Mock stimulation) for 4hrs and for 24 hrs for RNase Protection Assay, and they were stimulated with CGRP ($10^{-5}\;M$) and TNF-$\alpha$(2 ng/mL) for 24 hrs and with various concentraion of TNF-$\alpha$(2, 10, and 100 ng/mL) for Rnase Protection Assay with a human MMP-1 probe set including MMP 1, 2, 8, 7, 8, 9, 12, and TIMP 2, 3. In addition, cells (PF, GF and PDLF) were stimulated with Mock and various concentraion of TNF-$\alpha$(2, 10, and 100 ng/mL) for 24 hrs and with TNF-$\alpha$(10 ng/mL) for 48 hrs, and the supernatents from the cells were collected for Enzyme Linked Immunosorbent Assay (ELISA) for MMP-1 and MMP-13. Results: The expression of MMPs in PF, GF, PDLF after stimulation with SP and CGRP were not changed compared with Mock stimulation for 4 hrs and 24 hrs. The expression of MMP-1, -12, -13 24 hrs after stimulation with TNF-$\alpha$ were upregulated, however the expression of TIMP-3 in PF, GF, PDLF after stimulation with TNF-$\alpha$ were downregulated. TNF-$\alpha$(2 ng/mL, 10 ng/mL, 100 ng/mL) increased MMP-1 and MMP-12 expression in PF dose dependently for 24 hrs. Conclusions: TNF-$\alpha$ in the area of inflammation may play an important role in regulating the remodeling of dentin, cementum, and alveolar bone.

• Resources Recycling
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• v.9 no.6
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• pp.36-44
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• 2000

Fly ash was composed of the unburned carbon and mineral particles. The former was able to attach on the bubbles, while the latter was not. Therefore, it was possible to separate the unburned carbon and the mineral from fly ash using the froth flotation process. This study was carried out to evaluate the separation efficiency as a function of the ny ash particle properties in the column flotation. Separation efficiency was analyzed for various size fraction of -38 fm,38~125 fm and 1125 W, and for various fly ash samples containing 7, 11, and 20 wt% unburned carbon. For the size fractions of -38 fm containing 7 wt% unburned carbon, separation efficiency was 86ft, whereas separation efficiency was found to be 74% for the size fraction of +125$\mu\textrm{m}$ containing 20 wt% unburned carbon. The results indicated that separation efficiency increased with the decrease in the particle size and the unburned carbon content of the fly ash.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.9
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• pp.1139-1145
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• 2006

In this study, antioxidant activities of enzymatic and methanolic extracts from E. cava stem and leave were evaluated by measuring the scavenging activities on 1,1 diphenyl 2 picrylhydrazyl (DPPH), hydroxyl radical, hydrogen peroxide and the inhibitory effects on DNA damage induced by oxidative stress of cells. Enzymatic extracts were prepared by enzymatic hydrolysis of both stem and leave using food grade five different carbohydrases (Viscozyme, Celluclast, AMG, Termamyl, Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme, Alcalase). The enzymatic extracts were lower than methanolic extracts in polyphenol contents, but higher in extraction yield by approximately 30%. The enzymatic extracts were superior to methanolic extracts in DPPH and H2O2 scavenging activities and DNA damage protective effect. There were no significant antioxidant activity difference between stem and leave, but the extracts of leave were relatively better than those of stem. In this study it is suggested that E. cava stem as well as its leave would be a good raw materials for antioxidants compound extraction and enzymatic hydrolysis would be a good strategy to prepare antioxidant extracts from seaweeds.

• Journal of agriculture & life science
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• v.45 no.1
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• pp.59-66
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• 2011

This study was conducted to investigate the effect of vase material and floral preservative treatment over time on flower color, leaf color and flower size of cut flowers Rosa hybrida 'Aqua' and 'Corvernet', and Gerbera jamesonii 'Honeymoon' and 'Golden Time' stuck in a glass, porcelain, or onggi (pottery with a dark bronze glaze) vase containing either tap water or a floral preservative solution. The ${\Delta}E$ values in flower color of 'Aqua' rose at 8 days after treatment with a floral preservative in onggi and porcelain vases were low. The ${\Delta}E$ value of 'Covernet' rose treated with floral preservative in an onggi vase was the lowest and L value was the closest to that of petals of cut flowers at just before treatment (control). The ${\Delta}E$ value of 'Honeymoon' gerbera treated with a floral preservative in an onggi vase was the lowest and a value of 58.81 and b value of 34.29 were the closest to that of the control group as color of cut flowers in an onggi vase was similar to the color at the beginning of treatment. The ${\Delta}E$ value of 'Golden Time' gerbera treated in an onggi vase was significantly lower than that in a porcelain or glass vase and a value of -7.81 treated with a floral preservative solution in an onggi vase was the closest to the control and b value was high in an onggi vase as well. The L, a, and b values in leaf color of roses were similar to each value of the control and ${\Delta}E$ value of 3.25 measured in an onggi vase was lower than that in a porcelain or glass vase. Flower diameter of 'Covernet' and 'Golden Time' roses treated with a floral preservative in an onggi vase was greater than that in other treatments. From these results, the floral preservative applied to a holding solution is assumed to improve the quality and freshness of cut roses and gerberas by inhibiting microbes propagation and by promoting uptake of water and nutrients. The onggi vase with fine pores will promote the expression and maintenance of flower and leaf colors and may increase flower diameter by high air permeability.

• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.219-228
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• 2009

The construction procedures and artificial turf maintenance program on golf course definitely influence on the distortion of its environment. Soil microbial communities in soil profile were affected directly by those practises on turf areas. In Jeju island, the environmental impact assessment has been required to apply the first quality class granular activated carbon(GAC), which has a high absorbent character to agricultural chemicals, on the soil profiles of golf green system to reduce the pesticide leaching to ground water. This research was carried out to analyze the changes of microbial communities and chemical properties on soil profiles where GAC had been applied at the construction stage at two golf courses in Jeju. The changes of soil microbial population and chemical properties associated with construction methods of soil profile and agrochemical management program were analyzed by monthly at the surface and sub-soil profiles during April through October, 2007. The total numbers of bacteria and fungi, soil moisture content, soil physio-chemical properties were measured on greens and fairways of the both golf courses with different GAC treatment on the green and fairway soil profiles. The results showed that GAC had positive effects on the water holding capacity, pH and EC, however, it did not improved the holding capacity of available nutrients ${NO_3}^-,{NH_4}^+$, and phosphorus by its sorption phenomenon. In microbial count test, the total numbers of bacteria and fungi showed a great variation during sampling dates. That may directly relate to the agrochemical application, however, the ratio of total bacterial number versus total fungus number showed a constant value on a sub-soil of 15~30cm depth. Thus, the construction method of GAC in soil profile, and application of fertilizer and pesticide, both impacted on the changes of microbial population. It's means that the construction method of soil profile and turf management using agro-materials might greatly affect on the turfgrass culture and the environment of golf course.

• The Journal of Korean Academy of Prosthodontics
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• v.59 no.4
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• pp.395-404
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• 2021

Purpose. The purpose of present study was to evaluate the effect of changing structural stability of wax disc on the fit of prosthesis when the milling proceeded in order. Materials and methods. Prepared maxillary left first molar was used to fabricate a Ni-Cr alloy reference model. This was scanned to design crown and then wax pattern was milled, invested and cast to fabricate prosthesis. The wax patterns located in a row centrally within a single wax disc were set into a total of five groups ranging from WM1 group that was first milled to WM5 group that was last milled and the number of each group was set as 10. Silicone replica technique was used to measure the marginal gap, axial internal gap, line angle internal gap, occlusal internal gap. Data was evaluated with one-way ANOVA with significance level set at α = .05 and then Tukey HSD test was conducted for post analysis. Results. Marginal gap measured in each group, it was 40.41 ± 2.15 ㎛ in WM1 group, 40.44 ± 2.23 ㎛ in WM2 group, 39.96 ± 2.25 ㎛ in WM3 group, 39.96 ± 2.48 ㎛ in WM4 group, and 40.57 ± 2.53 ㎛ in WM5 group. No significant difference was found between groups. The significant difference between the groups was also not found in the axial internal gap, line angle internal gap, and occlusal internal gap. Conclusion. Internal and marginal fit of single crown to the sequential order of milling processing in the single machinable wax disc did not seem to be affected by the sequence.

• The Journal of Korean Academy of Prosthodontics
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• v.59 no.3
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• pp.291-298
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• 2021

Purpose. The purpose of this study is to compare five interdental cleansing products' effectiveness on removing artificial dental plaque on the interdental space of zirconia crowns. Materials and methods. A model with abutments on the right mandibular second premolar and first molar were prepared. 10 zirconia crowns for each abutment were fabricated. After applying artificial dental plaque between the zirconia crowns, a single clinician attempted to remove the plaque with five products: interdental toothbrush, end-tuft toothbrush, dental floss, Easypick, Water pik. They were conducted 10 times per group. The aspect and area of removed surfaces were analyzed using images taken with a digital camera. One factor analysis of variance was performed as a statistical analysis, and a post-hoc test was performed using the Scheffé method (P < .05). Results. There were differences in the area and the pattern according to the characteristics of the products. The largest area, including the marginal portion, was removed in the dental floss group. Interdental toothbrush group was the most effective in removing the dental plaque at the marginal portion. Easypick was less effective than the interdental toothbrush. The end-tuft toothbrush showed better results than other products in cleansing mesiobuccal and distobuccal area, but could not cleanse the area directly below the contact point. In Water pik group, artificial dental plaque was scarcely removed. The removal rate of artificial dental plaque was in the order of floss (69.47%), end-tuft toothbrush (49.36%), interdental toothbrush (44.20%), Easy pick (13.04%), and Water pik (0.59%). Conclusion. Dental floss showed the highest removal rate in the interdental space restored with zirconia crowns, while interdental toothbrush was the most effective in removing the dental plaque at the marginal portion.

• Korean Journal of Breeding Science
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• v.49 no.3
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• pp.150-156
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• 2017

This study was carried out to compare the survival and mutation rates and mutation spectrum by gamma-irradiation on rooted and unrooted cuttings of three spray type ('Lovelydia', 'Yellowbabe', and 'Haetsal') and two standard type ('Vital' and 'Aqua') cultivars in roses. Two groups, rooted and unrooted cuttings were gamma-irradiated at 70Gy for 24 hours. The irradiated rooted and unrooted cuttings were planted in a greenhouse, and survival, mutation rates and mutation spectrum were investigated 30 weeks after planting, respectively. As a result, survival and mutation rates of gamma-ray irradiated plants were 16.4%~50.8% and 0~5.1% for unrooted cuttings, and 39.4%~55.1% and 0.7%~7.4% for rooted cuttings, respectively. In conclusion, both survival and mutation rates were a little higher on rooted cuttings than on unrooted cuttings. However, when only survived plants after gamma-ray irradiation were considered, mutation rates were 0~10% and 1.8%~14.1% for unrooted cuttings and rooted cuttings, respectively, showing no significant difference. In addition, diverse variations on color and number of petals or shape of flowers were detected both in plants from rooted and unrooted cuttings, which indicated that there was no significant difference in mutation spectrum between two groups.

• Food Engineering Progress
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• v.15 no.4
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• pp.346-354
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• 2011

This study was carried out to fortify the antimicrobial activity of yoghurt by adding liquorice extract to it. The liquorice extracts (1 mg/mL) showed relatively high antibacterial activity against H. pylori KCCM 40449 (p < 0.05). The solvent liquorice extracts of minimal inhibitory concentrations (MIC) against H. pylori KCCM 40449 were 25- 100 ${\mu}g$/mL. Lactobacillus amylovorus DU-21 with high EPS production ability were inoulated to milk after the addition of different amounts of liquorice extracts (0.0%, 0.05%, 0.1% and 0.2%). The physico-chemical characteristics of yoghurts added with liquorice extracts were examined. The initial pH, titratable acidity, viscosity and viable cell counts of the yoghurt added liquorice extracts were 3.41-3.51, 1.021-1.091%, 1,686-1,930 cp and 9.41-9.38 Log CFU/mL, respectively. The viscosity and syneresis of yoghurt were better than that of the control. Antimicrobial activity against H. pylori KCCM 40449 increased with increasing addition of liquorice extract. However, the sensory score of yoghurt added with different amounts of liquorice extracts was lower than that of the control (p < 0.05). As a result of the sensory evaluations, the flavor, taste, texture, color and overall acceptability of the yoghurt with 0.05% liquorice extract were found to be much better than those of the other groups (p < 0.05). Overall, the optimal amount of liquorice extract added in the manufacture of yoghurt was 0.05% of the total weight. Further studies on increment of antimicrobial activity and palatability of liquorice extract added yoghurt are necessary.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.4
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• pp.93-99
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• 2019

The purpose of this study was to investigate the bond strength between PEKK(Polyetherketoneketone) and Sinfony(3M ESPE, Seefeld, Germany) the dental composite resin by proposing the three representative surface treatment methods and evaluate to see if they affect the bond strength between two materials. A total of 30 PEKK($Pekkton^{(R)}$ Ivory, $Cendres+M{\acute{e}}taux$, Bienne, Switzerland) specimens were prepared, embedded in acrylic resin, polished(P 1200 grid) to surface, and each group was divided into 10 specimens. After then, by the surface treatment method, it classified into three groups(n=10) such as Air abrasion group(PN), applying Single Bond Universal(3M ESPE) after Air abrasion(PB), applying OPAQUE(3M ESPE) after Air abrasion(PO). Then, veneering was performed by using Sinfony(3M ESPE, Seefeld, Germany). All completed specimens were allowed to rest in a $37^{\circ}C$ water bath for 24 hours. Shear bond strength of each group was measured and fracture patterns were classified. Statistic analysis was performed with One-way ANOVA followed by post hoc Scheffe tast (p<.05). Statistical analysis was performed using the SPSSWIN 21.0 program. As a result of one-way ANOVA, the average value of PB group was $27.67{\pm}4,18MPa$ and it was shown as the highest bond strength, PN and PO were $20.43{\pm}1.70$ and $19.8{\pm}4.77MPa$ each, and these were relatively low(F=18.4, P<.001), and as the post-test the Scheffe test was conducted and verified (p<.05). After examining the scheffe test, it was showed significant differences as PB>PO, PB>PN(p<.001). Through this study, in order to enhance the bonding force between PEKK and the composite resin, perform the Air abrasion and surface treatment by using Single Bond Universal(3M ESPE) is recommended, and as coMPared with other studies. And it is assumed that the increase of the application time of the Air abrasion affects the increase of the shear bond strength. Thus, further research is required.