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Early Time Course of Immunopathologic Changes in Coronary Arteries of Heterotopically Transplanted Mouse Heart (이소 생쥐심장이식 모델에서 조기 시간경과에 따른 관상동맥의 면역병리학적인 변화)

  • 이정렬;임홍국;서정욱
    • Journal of Chest Surgery
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    • v.31 no.12
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    • pp.1119-1126
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    • 1998
  • Background: In this study, we investigated the early time course of expression of the major histocompatibility(MHC) antigens, intercellular adhesion molecule-1(ICAM-1), vascular cell adhesion molecule-1(VCAM-1), interleukin-6 and the histopathological changes in the coronary arteries of cardiac allografts exchanged between inbred mice strains that differ in one loci of class I major histocompatibility antigen (B10.BR to B10.A). Material and Method: No immunosuppressive therapy was used. Both allografts and the hearts of the recipients were harvested at 7(group 1, n=6), 15(group 2, n=6), 21(group 3, n=6), and 30(group 4, n=6) days after transplantation. They were examined by immunohistochemistry, microscopy and morphometry. All allografts had contractions at the time of harvest. Result: A strong MHC class I antigen expression was present on the endothelial and medial cells of the coronary arteries in group 1 and remained unchanged in the rest of the groups. However, MHC class II reactivity was none or very little at any time. Mild to moderate ICAM-1 expression was observed on the endothelial cells, but not on the medial cells at any time by 30 days. VCAM-1 expression was strong both on the endothelial and medial cells at any time. Moderate degree expression of interleukin-6 was observed from 7 to 30 day specimens. Histopathologically, percentage of affected vessels(vessels with intimal thickening) was less than 10 % in 7 day group and increased up to 50 % at 30 days. Mean percent narrowing of the lumen of the affected vessels revealed less than 20 % at 7 days and 40 % at 30 days. The area occupied by tropomyosin positive cells in the intimal lesion, graded from 0 to 3, showed gradual increase but remained between grade 0 to 1 by 30 days. Medial integrity was also well preserved at any time. Moderate perivascular mononuclear cell infiltration was observed at 7 days and it was progressively increased upto 30 days. Recipients' heart revealed no positive immunopathologic findings. Conclusion: In this study, the early time course of progression of the transplantation vasculopathy was demonstrated in the murine heterotopic heart transplant model.

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A Function and Weight Selection of Ecosystem Service Function for the Eco-friendly Protected Horticulture Complex in Agricultural Landscape (시설원예단지의 친환경적 조성을 위한 생태계서비스 기능 및 가중치 산정)

  • SON, Jinkwan;KONG, Minjae;SHIN, Yukung;YUN, Sungwook;KANG, Donghyeon;Park, Minjung;LEE, Siyoung
    • Journal of Wetlands Research
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    • v.19 no.4
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    • pp.533-541
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    • 2017
  • Agricultural landscape has many ecosystem service functions. However, the development of the horticulture complex has no consideration for environmental conservation. Therefore, we analyzed the priorities of ecosystem service functions required for the composition. The study was conducted in three stages. As a result of the first survey, 17 functions were selected to be improved. In the second survey, 12 functions were selected excluding 5 functions. Finally, 1. Measures for water purification, 2. Groundwater recharge plan, 3. Surface water storage space, 4. Flood control measures, 5. Vegetation diversity space, 6. Carbon emission reduction plan, 7. Aquatic insect habitat space, 8. Amphibian reptiles 9. Landscape and Waste Management, 10. Bird Species Space, 11. Heat Island Mitigation Plan, 12. Experience / Ecological Education Plan. We proposed the structure, capacity, flow rate, arrangement and form of the water treatment facility to improve water quality by improving the function. We proposed a reservoir space of 7-10% for groundwater recharge. The development of reservoir and storage facilities suitable for the Korean situation is suggested for the surface water storage and flood control measures. And proposed to secure a green space for the climate cycle. Proposed habitat and nutrient discharge management for biodiversity. We propose green area development and wetland development to improve the landscape, and put into the facilities for experiential education. The results of the research can be utilized for the development and improvement of the horticultural complex.

Impact of the Rice-Duck Farming System on Regional Agricultural Environment at Hongsung Area (오리농법에 의한 벼 재배가 지역 환경에 미치는 영향 평가)

  • Roh, Kee-An;Kim, Min-Kyeong;Ko, Byong-Gu;Kim, Gun-Yeob;Shim, Kyo-Moon;Jeong, Hyun-Cheol;Lee, Deog-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.spc
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    • pp.57-61
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    • 2009
  • To clarify the impact of the rice-duck farming system on the regional environment and the surrounding, a case study was carried out at Hongdong Reservoir valley of Hongdong-myeon and Janggok-myeon, Hongseong-gun, Chungcheongnam-do where the density of livestock grazing is the highest and rice cultivation with the rice-duck farming system is extensively practiced. The soil characteristics and water qualities at paddy fields were compared between two rice cultivation methods of rice-duck farming system and conventional farming system. The organic matters and available phosphate contents in soil of paddy fields where the rice-duck farming system was practiced were higher than those of paddy fields where conventional farming system was practiced. However, the available phosphate content was lower than the optimum for rice cultivation and the mean concentration of paddy soil in Korea. The surface water quality of the paddy field with the rice-duck farming system was practiced had higher EC (137 %), $COD_{Cr}$ (220 %), T-N (172 %), and T-P (226 %) contents than that with the conventional farming system was practiced. Especially, $COD_{Cr}$ and T-P were more than 2 times higher, which tells that the possibility of water pollution by drainage water of paddy field is higher in the paddy fields with the rice-duck farming system practiced than in those with the conventional farming practiced. The higher contents of T-P and $COD_{Cr}$ in surface water at the paddy field of rice-duck farming system practiced were directly caused by soil particles in the muddy water. Consequently, it is necessary to thoroughly manage the irrigation and drainage system of rice-duck farming system practiced to prevent outflow of surface water from paddy and pollution of surrounding water system.

Warm Water Circulation and its Origin by Sea Level Fluctuation and Bottom Topography (해수면변화와 해저지형에 의한 난류수의 순환과 그 기원)

  • PARK Ig-Chan;OH Im Sang
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.5
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    • pp.677-697
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    • 1995
  • The analysis of long- period sea level variations with tidal record data around Korea, Japan, and Russia shows that about half of the variations are due to atmospheric influences. The sea level variation by water movements is the largest in the coasts along the Tsushima Current, and becomes smaller in the distant areas. It suggests that the sea level varications are related with the Tsushima Current. The effect of sea level variations to ocean circulation has been studied with a numerical model allowing barotropic sea level fluctuations, like the result with GCM (Semtner) model by Pang et al.(1993), the present model also shows that waters basically flow along isobaths over the last China Sea after geostyophic adjustment around Taiwan. However, barotropic sea level fluctuation makes the basic circulation in the Yellow Sea, which waters flow into the central Yellow Sea and out along the west coast of the Korean Peninsula. Besides this, barotropic sea level fluctuation makes long period waves over the shelf area as the Kuroshio varies. By the waves, the basic circulation in the Yellow Sea is disturbed, so that the flow pattern of oppositely flowing into the Yellow Sea along the west roast of the Korean Peninsula appears. In the Yellow Sea circulation, it seems that northwest winds strengthen the basic circulat ion In winter, and southeast winds strengthen the disturbed circulation in summer. Another point appeared by the long period wave is that the Tsushima Current possibly originates in different areas. There have been two opposing argues on the area in which the Tsushima Current originates the southwest sea of Kyushu Island and the adjacent sea of Taiwan. Through this study, we found that both of them seem to be important areas for the origin of the Tsushima Current, and one of them is possibly strengthened by long period waves. The long period waves given by the variation of the Kuroshio Current in the adjacent sea of Taiwan propagate to the Korea Strait as forced waves. The wave continuously propagates to the last Sea through the eastern channel, but reflects in the western channel due to bottom topography. The reflected waves propagate southwestward along the last China Sea as free waves and determine the sea level variations with forced waves.

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Eco-friendly remediation and reuse for coastal dredged materials using a bioaugmentation technology (생물증강법을 이용한 오염해양준설토의 환경친화적 정화 및 재활용)

  • Kim, In-Soo;Ha, Shin-Young;Koh, Sung-Cheol
    • Korean Journal of Microbiology
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    • v.51 no.4
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    • pp.374-381
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    • 2015
  • Occurrences of coastal dredged materials are ever increasing due to port construction, navigational course maintenance and dredging of polluted coastal sediments. Ocean dumping of the coastal dredged materials has become virtually prohibited as London Treaty will be enacted as of the year 2012. It will be necessary to treat and recycle the dredged materials that may carry organic pollutants and heavy metals in a reasonable and effective process: collection of the dredged materials, liquid and solid separation, and treatment of organic compounds and heavy metals. In this study we have developed a continuous bioreactor system that can treat a mixture of silt and particulate organic matter using a microbial consortium (BM-S-1). The steady-state operation conditions were: pH (7.4-7.5), temperature ($16^{\circ}C$), DO (7.5-7.9), and salt concentration (3.4-3.7%). The treatment efficiencies of SCOD, T-N and T-P of the mixture were 95-96%, 92-99%, and 79-97%. The system was also effective in removal of heavy metals such as Zn, Ni, and Cr. Levels of MLSS during three months operation period were 11,000-19,000 mg/L. Interestingly, there was little sludge generated during this period of operation. The augmented microbial consortium seemed to be quite active in the removal of the organic component (30%) present in the dredged material in association with indigenous bacteria. The dominant phyla in the treatment processes were Proteobacteria and Bacteroidetes while dominant genii were Marinobacterium, Flaviramulus, Formosa, Alteromonadaceae_uc, Flavobacteriaceae_uc. These results will contribute to a development of a successful bioremediation technology for various coastal and river sediments with a high content of organic matter, inorganic nutrients and heavy metals, leading to a successful reuse of the polluted dredged sediments.

GIS-based Disaster Management System for a Private Insurance Company in Case of Typhoons(I) (지리정보기반의 재해 관리시스템 구축(I) -민간 보험사의 사례, 태풍의 경우-)

  • Chang Eun-Mi
    • Journal of the Korean Geographical Society
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    • v.41 no.1 s.112
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    • pp.106-120
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    • 2006
  • Natural or man-made disaster has been expected to be one of the potential themes that can integrate human geography and physical geography. Typhoons like Rusa and Maemi caused great loss to insurance companies as well as public sectors. We have implemented a natural disaster management system for a private insurance company to produce better estimation of hazards from high wind as well as calculate vulnerability of damage. Climatic gauge sites and addresses of contract's objects were geo-coded and the pressure values along all the typhoon tracks were vectorized into line objects. National GIS topog raphic maps with scale of 1: 5,000 were updated into base maps and digital elevation model with 30 meter space and land cover maps were used for reflecting roughness of land to wind velocity. All the data are converted to grid coverage with $1km{\times}1km$. Vulnerability curve of Munich Re was ad opted, and preprocessor and postprocessor of wind velocity model was implemented. Overlapping the location of contracts on the grid value coverage can show the relative risk, with given scenario. The wind velocities calculated by the model were compared with observed value (average $R^2=0.68$). The calibration of wind speed models was done by dropping two climatic gauge data, which enhanced $R^2$ values. The comparison of calculated loss with actual historical loss of the insurance company showed both underestimation and overestimation. This system enables the company to have quantitative data for optimizing the re-insurance ratio, to have a plan to allocate enterprise resources and to upgrade the international creditability of the company. A flood model, storm surge model and flash flood model are being added, at last, combined disaster vulnerability will be calculated for a total disaster management system.

The Fluctuation of Marine Aerosol Number Concentrations Related with Vertical Winds (연직풍에 따른 해양성 에어러솔 수 농도 변동에 관한 연구)

  • Park, Sung-Hwa;Jang, Sang-Min;Jung, Woon-Seon;Jeong, Jong-Hoon;Lee, Dong-In
    • Journal of the Korean earth science society
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    • v.33 no.3
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    • pp.259-268
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    • 2012
  • To investigate the fluctuation of marine aerosol number concentration at each different size with vertical winds in ocean area, aerosol particles and vertical wind components were measured in the Ieodo Ocean Research Station, which is located to 419 km southwest of Marado, the southernmost island of Korea, from 8 to 22 June 2009. The Laser Particle Counter (LPC) and ultrasonic anemometer were used to measure the number of aerosol particles and vertical wind speed. Surface weather chart, NCEP/NCAR reanalysis data and sounding data were used to analyze the synoptic condition. The distribution of aerosol number concentration had a large fluctuation of bigger particles more than 1.0 ${\mu}m$ in diameter by vertical wind speed during precipitation. The aerosol particles larger than 1.0 ${\mu}m$ in diameter increased as the wind changed from downward to upward during precipitation. The aerosol number concentration of bigger size than 1.0 ${\mu}m$ in diameter increased about 5 times when vertical velocity was about 0.4 $ms^{-1}$. In addition, the accumulation and coarse mode aerosol number concentration decreased about 45% and 92%, respectively compared to concentrations during precipitation period. It is considered that vertical wind plays an important role for the increasing of coarse mode aerosol number concentration compared to the large aerosol particles sufficiently removed by the scavenging effect of horizontal winds. Therefore, the upward vertical winds highly contribute to the formation and increase in aerosol number concentration below oceanic boundary layer.

Laparoscopy-assisted Total Gastrectomy for Advanced Upper Gastric Cancer - Comparison with Open Total Gastrectomy - (진행위암의 복강경 보조 위 전절제술 - 개복 위 전절제술과의 후향적 비교 -)

  • Lee, Jun Hyun;Nam, Yoo Hee;Hur, Hoon;Jeon, Hae Myung;Kim, Wook
    • Journal of Gastric Cancer
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    • v.8 no.3
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    • pp.141-147
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    • 2008
  • Purpose: The aim of this study was to compare the short-term operative outcomes of laparoscopy-assisted total gastrectomy (LATG) with those of open total gastrectomy (OTG) for patients suffering with advanced upper gastric cancer. Materials and Methods: Of the 47 patients who underwent LATG with $D1+{\beta}$ or D2 lymphadenectomy from July 2004 to March 2008, 29 patients with pathologically proven advanced gastric cancer were compared with 35 patients who underwent conventional OTG during the same time period. The comparison was based on the clinicopathological characteristics, the surgical outcome, the follow-up survival and tumor recurrence. Results: The patients' age, gender and body mass index were similar between the two groups. However, there were statistically differences in tumor size ($9.2{\pm}3.9$ vs $6.1{\pm}3.6cm$, P=0.002) and the proximal resected margin ($2.1{\pm}2.0$ vs $3.6{\pm}2.1cm$ P=0.004). There was no significant difference in most of the peri- and post-operative courses such as the time to first flatus, the time to starting a solid diet and the length of the hospital stay, except for a longer operating time (289.0 vs. 361.3 minutes, P<0.001) in the LATG group. The complication rate was higher in the LATG group (13.8%) than that in the OTG group (5.7%). The mean overall survival and disease free survival times were 32 and 31 months, and 24 and 28 months, respectively, with an average 18.8 months follow-up duration. The main recurrent sites were peritoneum and lymph node in both groups. Conclusion: The early results of the current study suggest that LATG for AGC is technically feasible and it does not show any inferiorities of the postoperative outcomes as compared to those of conventional open total gastrectomy.

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Antioxidant Activity of Extracts and Fractions from Aster scaber (참취 추출물과 용매분획물의 항산화 활성)

  • Jeon, Sang-Min;Lee, Jin-Young;Kim, Heon-Woong;Lee, Young-Min;Jang, Hwan-Hee;Hwang, Kyung-A;Kim, Haeng-Ran;Park, Dong-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.9
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    • pp.1197-1204
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    • 2012
  • As an effort to develop functional food ingredients and to discover the biological activity, the total phenolic content, total flavonoid content, DPPH and ABTS radical scavenging activity, SOD-like activity, ferric reducing antioxidant power (FRAP), and $Fe^{2+}$ chelating of Aster scaber were measured using a 70% ethanol extract and various solvent fractions. As a result, the total phenolic concent was highest in an ethyl acetate fraction of 141.9 mg GAE eq/g and the total flavonoid content was 105.6 mg QUE eq/g. The DPPH radical scavenging activity was highest in an ethyl acetate fraction of 97.1% at a concentration of 1,000 ${\mu}g/mL$ (p<0.05). The ABTS radical scavenging activity showed a 86.9% ethyl acetate fraction and a 57.9% butanol fraction at a concentration of 125 ${\mu}g/mL$, and higher than that of positive control (${\alpha}$-tocopherol and BHT) (p<0.05). The SOD-like activity showed 42.8% in an ethyl acetate at a concentration of 1,000 ${\mu}g/mL$. The ethyl acetate fraction showed the highest value of FRAP at 1051.9 ${\mu}M$ and a concentration of 1,000 ${\mu}g/mL$ (p<0.05). The $Fe^{2+}$chelating was highest in the 70.1% chloroform fraction at a concentration of 500 ${\mu}g/mL$ (p<0.05). There is the highest correlation between DPPH radical scavenging activity and FRAP (r=0.981) as compared to other antioxidant assays (p<0.01). With these results, we confirmed that the ethyl acetate fraction of Aster scaber has great antioxidant potential. So it can be expected to be developed into a specific functional food ingredient.

A Basic Study to Establish a Framework Act on Landscape Architecture (조경 기본법 제정을 위한 기초 연구)

  • Shin, Ick-Soon;Koo, Bon-Hak;Byeon, Jae-Sang
    • Journal of the Korean Institute of Landscape Architecture
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    • v.39 no.6
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    • pp.86-97
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    • 2011
  • This study analyzed the necessity of the tentatively-named "Framework Act on Landscape Architecture" as an umbrella law in the field of landscape architecture regarding legal, industrial, and academic aspects and is intended to provide basic data to suggest the optimal legal system and legislative proceedings to control it. This study can be summarized as follows: First, a form of framework act on landscape architecture is appropriate for a compromise between ideal type and political framework. Some content is suitable for proclamatory character including present and future issue related to the landscape architecture fields. Second, legislative proceedings are more reasonable as motions coming from assembly proposals rather than government. Motions by assembly proposal recommend the following procedure: submission of a legislative bill by an assemblyperson, passing of a permanent commission, review by government, leading approval by emphasis on correspondence with the principle to carry out government affairs, proclamation. Third, a frame based on content and form can be made up of a total of 7 chapters 34 articles. The function must include the following: the suggestion of direction for government policy, the systematization of the institution and what it includes, the control of government administration, a public service system regarding public relations for landscape architecture and so on. The results of this study will form a social consensus about the necessity of a framework act regarding landscape architecture fields and contribute to informing the importance of landscape architecture as related to other industrial fields.