• Journal of the Korea Convergence Society
• /
• v.8 no.5
• /
• pp.265-273
• /
• 2017

This study is convergence study to identify the relationships among attitude toward death(ATD), spiritual well-being(SW) and awareness about organ donation(AAOD). The survey was performed on 214 nursing students in one university. Data was collected using a structured questionnaires and analysed with PASW 20.0. Study findings revealed that AAOD has a significant correlation with ATD and SW. ATD(${\beta}=-.159$), SW(${\beta}=.132$), idea about brain death's organ donation(${\beta}=.314$), and major satisfaction(${\beta}=.134$) about AAOD were most significant predictive variables. These variables accounted for 20.5% of the variance in AAOD. The findings indicate the necessity of developing educational programs to enhance nursing students's ATD and SW for increasing positive AAOD and further rate of organ donation, and follow-up study.

• Korean Journal of Adult Nursing
• /
• v.28 no.4
• /
• pp.355-366
• /
• 2016

Purpose: The purpose of study was to describe operation room nurses' ethical values in relationship to the attitude and meaning of life toward organ transplantation in brain death. Methods: This study used a descriptive correlational survey design. Participants were 174 nurses who had attended to organ transplantation surgery in brain death more than once. Data were collected from September 1 to 11, 2015 and were analyzed using independent t-test, ANOVA, $Scheff{\acute{e}}$ test, Pearson's correlation coefficients, and hierarchical multiple regression with SPSS 22.0. Results: Educational level, attitude on organ transplantation in brain death, and meaning of life were significant variables predicting the level of nursing ethical value, accounting for 82.6% of the variability. Conclusion: Continued education and self-development programs should be encouraged for operating room nurses to establish professional nursing ethics as well as positive meaning of life and attitude toward the organ transplantation in brain death.

• The Journal of Korean Academic Society of Nursing Education
• /
• v.14 no.2
• /
• pp.216-223
• /
• 2008

Purpose: The purpose of this study was to discover attitudes toward organ donation in brain dead donors and nursing ethical values of staff nurses. Method: The subjects were 196 nurses with direct patient care in a university hospital. Data collection was carried out for one month in September, 2008 using structured questionnaire. The data were analyzed using frequency, percentage, mean and standards deviation, ANOVA and Pearson's Correlation with SPSS program. Result: The mean score of a positive attitude toward organ donation in brain dead donors was 3.59 points, and a negative attitude score was 3.02 points. The mean score of nursing ethical value was 3.05 points. The age and the length of career had a reverse correlation with a negative attitude toward organ donation. Nursing ethical values had a positive correlation with a negative attitude toward organ donation. Conclusion: It is necessary to increase understanding and recognition of brain death organs donation in the nurses who participate in direct patient care so as to help facilitate organ donation.

• Korean Journal of Culture and Social Issue
• /
• v.23 no.1
• /
• pp.1-24
• /
• 2017

This article aimed to test whether specific social values (i.e., achievement vs. benevolence) could cause changes in decision making on bio-ethical issues. Study 1 investigated value preferences among young Korean college students according to Schwartz's (1992) model of social values. According to the results, the preference for achievement value was correlated negatively with the preference for benevolence value. In Study 2, following a sentence completion task which was conducted to trigger specific values, the participants had to indicate their ethical decisions regarding animal experiment, euthanasia, organ transplantation, biotechnology, sex selection and human cloning, Irrespective of the value priming (achievement vs. benevolence), there were more utilitarian decisions about animal experiment, euthanasia and organ transplantation. In contrary, there were more deontological decisions about sex selection and human cloning. Study 3 introduced a word completion task to assess implicit value preferences. The results showed that the participants with implicit preferences for the benevolence value in the condition of benevolence value priming were more frequently against animal experiments and organ transplantation than those with implicit value preferences for the achievement value. Social values are discussed for understanding one's bioethical decision making.

• Journal of Industrial Convergence
• /
• v.19 no.4
• /
• pp.49-55
• /
• 2021

This study attempted to review the research on biomedical ethics of nursing students published in Korea. Keywords included 'nursing students', and 'biomedical ethics', and a total of 26 studies were collected via databases such as KISS, NDSL, RISS. The biomedical ethics awareness was the main concept of biomedical ethics, consisted of right to life of fetus, artificial insemination, organ transplantation, and so on. There were differences in biomedical ethics awareness by ethical education experience, grade, clinical practice experience, and ethical education willingness to attend. Also, major keywords analysed with biomedical ethics were withdrawal of life-sustaining treatment, critical thinking, sexual attitude, nursing professionalism, and death perception. Study results can be used to provide basic data for preparing nursing ethics education in the future.

• Journal of Chest Surgery
• /
• v.31 no.9
• /
• pp.884-892
• /
• 1998

Background: The gastroepiploic artery is not only an alternative graft but also may be considered an important primary graft for coronary revascularization. However, the long-term patency of the gastroepiploic arterial graft is yet to be determined and the incidence of perioperative spasm and long-term patency of a coronary graft may be affected by the properties of the graft response to certain vasoactive substances. The reactivity of the gastroepiploic artery to vasoactive substances has not been studied extensively and the results of the studies are contradictory. Material and Method: This study was designed to test the reactivity of human gastroepiploic artery to four constrictors and four relaxants. The middle sections of the human gastroepiploic arteries were collected from the patients undergoing gastrectomy and the arterial rings with intact endothelium were suspended in organ baths for isometric tension recording. Result: Epinephrine, norepinephrine, and potassium chloride induced the maximum constriction to higher forces (7.0$\pm$1.1g, 6.6$\pm$0.9g, and 6.5$\pm$1.1g) than 5-hydroxytryptamine did (3.8$\pm$1.7g, p<0.05). Nitroprusside and histamine induced almost full relaxation in the gastroepiploic arteries preconstricted with norepinephrine. There was no significant difference between two relaxants regarding maximum relaxation force. Acetylcholine induced the maximum relaxation to weaker force when compared with nitroprusside and histamine (p<0.05), and isoproterenol was the weakest of the relaxants (p<0.05 compared with acetylcholine). Conclusion: The gastroepiploic artery has a strong capacity of endothelium-dependent relaxation which could have an important influence on long-term patency. The gastroepiploic artery exhibits a potent contractility to catecholamines and the enhanced contractility may facilitate vasospasm in the presence of high circulating levels of catecholamines. Nitroprusside, a potent relaxant in gastroepiploic artery, might be beneficial for the treatment of gastroepiploic arterial graft spasm. The gastroepiploic arterial graft with intact endothelium may respond weakly to beta-adrenoceptor agonist and 5-hydroxytryptamine.

• Journal of Chest Surgery
• /
• v.29 no.8
• /
• pp.816-821
• /
• 1996

Lung transplantation is the established treatment for the end stage lung disedse find preservation of the organ is a major obstacle In performing lung transplantation. For solving this problem, we evaluated the histopathologic changes for various preservation solutions. Male mongrel dogs of similar size and weight (15∼20 kg) were used. The dog lungs were flushed with 4fl normal saline(group 1 'n:5): Modified Euro-Collins solution(group 2 n:5) and University of Wisconsin solution (group 3 : n=6), 60m11kg through a catheter placed in the main pulmonary artery aft r flushing of PGE 1 (20ng1kg). The lungs were preserved for 60 hours and measured dry and wet weights. Histologic specimens were taken every 6 hours and %toed for light microscopic evaluation. The edema ratio of the lungs peaked in 12 hours although there was no difference between the groups. Histologically, alveolar septal changes developed in one case (20%) after 1 hour preservation with normal saline. In case of the University of Wisconsin solution, the alveolar septal distortions and swellings were seen in 1 cases (20%) after 6 hours preservation compared with 3 cases (60%) after 6 hours preservation with Modified Euro-Collins solution. Changes of the pneumocytes were observed after 24 hours preser- vation in group 1, after 48 hours preservation in group 2 and after 60 hours preservation in group 3. We conclude that University of Wisconsin solution might have a superior preservation effect compare to normal saline and Modified Euro-Collins solutions.

• Korean Journal of Food Science and Technology
• /
• v.50 no.4
• /
• pp.451-456
• /
• 2018

The current study was conducted to confirm the anti-cancer effect of Sarijang, which is a mixture of extracts from purple bamboo salt, Rhynchosia nulubilis, garlic, and Ulmi cortex. Nude mice were injected with a human-derived colorectal cancer cell (HCT116 cell line) and subsequently administered Sarijang for 4 weeks, following which the body weight, organ weight, and tumor size were measured. To evaluate the anti-cancer mechanism of Sarijang, the levels of p16 and extracellular signal-regulated kinase (ERK), cell cycle regulators in colorectal cancer, were measured. To evaluate the toxicity of Sarijang on liver and kidney, aspartate transaminase, alanine transaminase, blood urea nitrogen, and creatinine were analyzed. Sarijang not only reduced the tumor size by enhancing p16 and suppressing ERK, but also showed no side-effect in the liver and kidneys. Taken together, Sarijang has the potential to inhibit tumor growth without side effects, and may be used as a useful functional food.

• The Korean Journal of Nuclear Medicine
• /
• v.35 no.3
• /
• pp.168-184
• /
• 2001

Purpose: KR-30035 (KR), a new MDR reversing agent, has been found to produce a similar degree of increased Tc-99m MIBI uptake in cultured tumor cells over-expressing mdr1 mRNA compared to verapamil (VP), with less cardiovascular effects. We assessed the MDR-reversing ability of KR in vivo, and effects of various doses of KR on MIBI uptake un nude mice hearing P-glycoprotein (P-gp) positive (+) and P-gp negative (-) human tumor xenografts. Methods: P-gp (+) HCT15/CL02 colorectal and P-gp (-) A549 non-small cell cancer cells were inoculated in each flank of 120 nude mice (20 mice ${\times}$ 6 groups). Group 1 (Gr1) mice received 10mg/kg KR i.p. 3 times $({\times}3)$; Gr2, 10mg/kg VP i.p. ${\times}3$; Gr3, 10mg/kg KR i.p. ${\times}2$ + 25mg/kg KR i.p. ${\times}1$; Gr4, 10mg/kg KR i.p. ${\times}2$ + 50mg/kg i.p. ${\times}1$; Gr5, 10mg/kg KR i.p. ${\times}2$ + 25mg/kg KR i.v. ${\times}1$, GrC, controls. The mice were then injected with Tc-99m MIBI and sacrificed after 10 min, 30 min, 90 min and 240 min. Tumor uptake of MIBI (TU) in each group was compared. Results: TU in P-gp (+) and (-) tumors were both higher in Gr1 than Gr2. Washout rate between the 10 min and 4 hours was lower in Gr5 of P-gp (+) cell(0.93) than the control. Percentage increases in TU were higher in P-gp (+) than P-gp (-) tumors with all KR doses. Pgp (+) TU were highest at 10 mon (173% of GrC) and persisted up to 240 min (144%) in Gr3. Larger doses of KR resulted in a lesser degree of increase in P-gp (+) TU at 10 min (130% in Gr4 and 117% un Gr5) and 30 min (178%, 129%), but TU increased by time up to 240 min (177%, 196%). Heart and lung uptakes were markedly increased in Gr4 and Gr5 at 10 and 30 min, likely due to cardiovascular effects. No mice died. Conclusion: These data further suggest that KR that has significantly lower cardiovascular toxicity than verapamil can be used as an active inhibitor of MDR. Even a relatively low dose of KR significantly increased Tc-99m MIBI uptake in P-gp (+) tumors in vivo.

• The Korean Journal of Nuclear Medicine
• /
• v.39 no.1
• /
• pp.34-43
• /
• 2005

Purpose: $^{99m}Tc$-sestamibi(MIBI) and $^{99m}Tc$-tetrofosmin have been used as substrates for P-glycoprotein (Pgp) and multidrug resistance associated protein (MRP), which are closely associated with multidrug resistance of the tumors. To understand different handling of radiotracers in cancer cell lines expressing Pgp and MRP, we compared cellular uptakes of $^{99m}Tc$-MIBI and $^{99m}Tc$-tetrofosmin. The effects of cyclosporin A (CsA), well-known multidrug resistant reversing agent, on the uptake of both tracers were also compared. Materials and Methods: HCT15/CL02 human colorectal cancer cells for Pgp expressing cells, and human non-small cell lung cancer A549 cells for MRP expressing cells, were used for in vitro and in vivo studies. RT-PCR, western blot analysis and immunohistochemistry were used for detection of Pgp and MRP. MDR-reversal effect with CsA was evaluated at different drug concentrations after incubation with MIBI or tetrofosmin. Radioactivities of supernatant and pellet were measured with gamma well counter. Tumoral uptake of the tracers were measured from tumor bearing nude mice treated with or without CsA. Results: RT-PCR, western blot analysis of the cells and irnrnunochemical staining revealed selective expression of Pgp and MRP for HCY15/CL02 and A549 cells, respectively. There were no significant difference in cellular uptakes of both tracers in HCT15/CL02 cells, but MIBI uptake was slightly higher than that of tetrofosmin in A549 cells. Co-incubation with CsA resulted in a increase in cellular uptakes of MIBI and tetrofosmin. Uptake of MIBI or tetrofosmin in HCT15/CL02 cells was increased by 10- and 2.4-fold, and by 7.5 and 6.3-fold in A549 cells, respectively. Percentage increase of MIBI was higher than that of tetrofosmin with CsA for both cells (p<0.05). In vivo biodistribution study showed that MIBI (114% at 10 min, 257% at 60 min, 396% at 240 min) and tetrofosmin uptake (110% at 10 min, 205% at 60 min, 410% at 240 min) were progressively increased by the time, up to 240 min with CsA. But increases in tumoral uptake were not significantly different between MIBI and tetrofosmin for both tumors. Conclusion: MIBI seems to be a better tracer than tetrofosmin for evaluating MDR reversal effect of the modulators in vitro, but these differences were not evident in vivo tumoral uptake. Both MIBI and tetrofosmin seem to be suitable tracers for imaging Pgp- and MRP-mediated drug resistance in tumors.